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Gastrointestinal tuberculosis:An autopsy-based study

Infectious Medicine 2023年第2期2卷 122-127页

作者： Julio Cesar Mantilla juan Joséchaves Ferney Africano-Lopez Néstor Blanco-Barrera Marta Juliana Mantilla Department of Pathology Universidad Industrial de SantanderBucaramanga 680002Colombia Department of Pathology Hospital Universitario de SantanderBucaramanga 680002Colombia Department of Pathology Fundación Universitaria de Ciencias de la SaludBogotá111411Colombia Department of Gastroenterology Gastrocenter and Medical SpecialitiesIpiales 524061Colombia Department of Gastroenterology Universidad Militar Nueva GranadaBogotá250240Colombia Department of Rheumatology Universidad de la SabanaChía 250001Colombia

Background:Tuberculosis is a disease of great relevance since it is one of the leading causes of morbidity and mortality *** tuberculosis is an unusual *** is defined as the involvement of any segment of the digestive tract,associated viscera,and *** study’s main objective is to collect information from autopsies of patients diagnosed with gastrointestinal tuberculosis in a Pathology reference center in ***:This is a retrospective and descriptive study of autopsy reports.A total of 4,500 autopsies were performed between January 2004 and December *** inclusion criteria were authorization of a family member following local law regulations and a final autopsy diagnosis of gastrointestinal tuberculosis using microscopic ***:Forty-eight patients with gastrointestinal tuberculosis autopsies were included in our *** of the patients were male(n=35,72.9%)with a median age of 40.5 years *** immunodeficiency virus infection history was reported in 28 cases(58.33%).The most affected gastrointestinal tract site was the terminal *** and thickened epithelium were common autopsies macroscopic *** multiorgan compromise was a relevant finding in patients with gastrointestinal ***:Gastrointestinal tuberculosis is a disease of great importance,being its diagnosis a clinical *** can be reported in a high percentage of cases,so autopsy diagnosis can help reveal more accurate data about this condition.

关键词： Gastrointestinal tuberculosis Autopsy Forensic pathology Infections Colombia

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Quality evaluation of Nothofagus pumilio seeds linked to forest management and climatic events

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Ecological Processes 2024年第1期13卷 98-107页

作者： Julian Rodríguez-Souilla Jimena E.chaves María Vanessa Lencinas juan Manuel Cellini Fidel A.Roig Pablo L.Peri Guillermo Martinez Pastur Laboratorio de Recursos Agroforestales Centro Austral de Investigaciones Científicas (CADIC) Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET ) Laboratorio de Investigaciones en Maderas (LIMAD) Universidad Nacional de la Plata (UNLP) Instituto Argentino de Nivología Glaciología y Ciencias Ambientales (IAN-IGLA) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Instituto Nacional de Tecnología Agropecuaria (INTA) Universidad Nacional de la Patagonia Austral (UNPA) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET )

Background Forest ecosystems undergo significant transformations due to harvesting and climate fluctuations, emphasizing the critical role of seeding in natural regeneration and long-term structural preservation. Climate change further amplifies these dynamics, affecting phenology across species and regions. In Tierra del Fuego(Argentina), Nothofagus pumilio(lenga) forests represent the most important timber resource, and it is managed through different silvicultural strategies. This species demonstrates notable post-disturbance regeneration, yet seed fall exhibits signifi-cant variability, leading to variations in seed quality(e.g., viability). This study aims to assess fluctuations in N. pumilio seed quality, determine how it varies concerning forest management strategies, annual productivity, and the co-occurrence of climatic phenomena including El Ni?o-Southern Oscillation(ENSO) and the Southern Annular Mode(SAM).Results Viable seeds represented 18.4% of the total, notably higher in unharvested than in managed areas. Con-versely, empty seeds were more prevalent in harvested areas(> 75%). Seed quality exhibited significant differences across silvicultural treatments, except for insect-predated seeds, which had similar proportions across all areas, though dispersed retention showed higher predation. When considering years with varying production levels, high-production years favoured full and viable seeds, particularly in unharvested forests and aggregated retention, while low-production years saw reduced viability across all treatments. Quadratic models revealed that viability increased with seed production, where unharvested forests achieved the highest values. Climate variability influenced seed proportions, where ENSO+/SAM+ promoting more full and viable seeds, while ENSO–/SAM+ favoured non-predated seeds, especially in unharvested *** Seed quality varies among treatments and years with different levels of seeding. Variations in seed qual-ity, l

关键词： Forest management Seeding Climate change Extreme event Patagonia

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Effect of Using Two Different Types of Carbon Nanotubes for Blackberry (Rubus adenotrichos) in Vitro Plant Rooting, Growth and Histology

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American Journal of Plant Sciences 2014年第24期5卷 3510-3518页

作者： Dora Flores Randall Chacón Luis Alvarado Alexander Schmidt Carlos Alvarado juan chaves Centro de Investigación en Biotecnología Instituto Tecnológico de Costa Rica Cartago Costa Rica Laboratorio de Nanotecnología Instituto Tecnológico de Costa Rica Cartago Costa Rica

Nanoparticles are able to interact with biomolecules, creating functional nanosystems for transportation within in vivo cells, and leading to the study of their potential applications in the field of plant biotechnology. Therefore, the aim of this research was to determine the growth and rooting effect of functionalized (SWCNTs-COOH) and non-functionalized nanoparticles with iron residue inner particles (SWCNTs-Fe) in blackberry (Rubus adenotrichos) in vitro plants. Two types of SWCNTs were used, both of them characterized in a solid sample through Raman spectroscopy (λ = 532 nm) showing differences in the G band between SWCNT + Fe and SWCNT + COOH. The in vitro plants (approximately 15 mm length) were inoculated in a rooting medium. Six treatments were established: 4, 8, 12 μg/ml for each type of SWCNTs and a control without nanotubes. The assessed variables consisted of the average number of days for root emergence, average number of roots per plant, average root length per plant and the average stem length. This study determined that, in general, the SWCNTs-COOH promoted the growth of the in vitro plants under this assay, when compared to the SWCNTs-Fe trials. The lowest SWCNTs-COOH dose evidenced the best results for the assessed variables. Additionally, the histological analysis also evidenced that the plants treated with SWCNTs-COOH nanotubes (4 μg/ml) increased their cellular metabolism when compared to the control group.

关键词： Single Walled Carbon Nanotubes Growth Rooting Blackberry TEM

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Soil organic carbon stocks in native forest of Argentina:a useful surrogate for mitigation and conservation planning under climate variability

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Ecological Processes 2024年第1期13卷 3-24页

作者： Pablo L.Peri juan Gaitán Matías Mastrangelo Marcelo Nosetto Pablo E.Villagra Ezequiel Balducci Martín Pinazo Roxana P.Eclesia Alejandra Von Wallis Sebastián Villarino Francisco Alaggia Marina González Polo Silvina Manrique Pablo A.Meglioli Julián Rodríguez-Souilla Martín Mónaco Jimena E.chaves Ariel Medina Ignacio Gasparri Eugenio Alvarez Arnesi María Paula Barral Axel von Müller Norberto M.Pahr Josefina Uribe Echevarria Pedro Fernández Marina Morsucci Dardo López juan Manuel Cellini Leandro Alvarez Ignacio Barberis Hernán Colomb Ludmila La Manna Sebastián Barbaro Cecilia Blundo Ximena Sirimarco Laura Cavallero Gualberto Zalazar Guillermo Martínez Pastur Instituto Nacional de Tecnología Agropecuaria (INTA) Universidad Nacional de la Patagonia Austral (UNPA) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Universidad Nacional de Luján Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Grupo de Estudio de Agroecosistemas y Paisajes Rurales (GEAP) Universidad Nacional de Mar del Plata Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET ) CCT San Luis Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Instituto Argentino de Nivología Glaciología y Ciencias Ambientales (IANIGLA) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Universidad Nacional de Cuyo Instituto de Investigaciones en Biodiversidad y Medioambiente (INIBIOMA) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Instituto de Investigaciones en Energía No Convencional CCT Salta-JujuyConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Laboratorio de Recursos Agroforestales Centro Austral de Investigaciones Científicas (CADIC) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Dirección Nacional de Bosques Ministerio de Ambiente y Desarrollo Sostenible de la Nación Instituto de Ecología Regional Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET )Universidad Nacional de Tucumán Universidad Nacional de Rosario Instituto de Investigaciones en Ciencias Agrarias de Rosario Centro Científico Tecnológico CONICET Grupo de Estudio de Agroecosistemas y Paisajes Rurales (GEAP) Universidad Nacional de Mar del Plata Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Laboratorio de Investigaciones en Maderas (LIMAD) Universidad Nacional de La Plata (UNLP) Administración de Parques Nacionales (APN) Parque Nacional Los Alerces Centro de Estudios Ambientales Integrados (CEAI) Universidad Nacional de la Patagonia San Juan Bosco (UNPASJB) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET )

Background The nationally determined contribution（NDC） presented by Argentina within the framework of the Paris Agreement is aligned with the decisions made in the context of the United Nations Framework Conven-tion on Climate Change（UNFCCC） on the reduction of emissions derived from deforestation and forest degradation, as well as forest carbon conservation（REDD+）. In addition, climate change constitutes one of the greatest threats to forest biodiversity and ecosystem services. However, the soil organic carbon（SOC） stocks of native forests have not been incorporated into the Forest Reference Emission Levels calculations and for conservation planning under cli-mate variability due to a lack of information. The objectives of this study were:（i） to model SOC stocks to 30 cm of native forests at a national scale using climatic, topographic and vegetation as predictor variables, and（ii） to relate SOC stocks with spatial–temporal remotely sensed indices to determine biodiversity conservation concerns due to threats from high inter-annual climate *** We used 1040 forest soil samples（0–30 cm） to generate spatially explicit estimates of SOC native forests in Argentina at a spatial resolution of approximately 200 m. We selected 52 potential predictive environmental covari-ates, which represent key factors for the spatial distribution of SOC. All covariate maps were uploaded to the Google Earth Engine cloud-based computing platform for subsequent modelling. To determine the biodiversity threats from high inter-annual climate variability, we employed the spatial–temporal satellite-derived indices based on Enhanced Vegetation Index（EVI） and land surface temperature（LST） images from Landsat *** SOC model（0–30 cm depth） prediction accounted for 69% of the variation of this soil property across the whole native forest coverage in Argentina. Total mean SOC stock reached 2.81 Pg C（2.71–2.84 Pg C with a probability of 90%） for a total area of 460,790 km2,

关键词： Soil organic carbon Biodiversity Native forest Climate change mitigation Modelling Resilience

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Carbon pool dynamics after variable retention harvesting in Nothofagus pumilio forests of Tierra del Fuego

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Ecological Processes 2023年第1期12卷 64-82页

作者： Jimena E.chaves Marie-Claire Aravena Acuña Julián Rodríguez-Souilla juan M.Cellini Nolan J.Rappa María V.Lencinas Pablo L.Peri Guillermo J.Martínez Pastur Centro Austral de Investigaciones Científicas(CADIC) Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET)Houssay 200(9410)UshuaiaTierra del FuegoArgentina Laboratorio de Investigaciones en Maderas(LIMAD) Universidad Nacional de la Plata(UNLP)Diagonal 113469(1900)La PlataBuenos AiresArgentina Nature Conservation&Landscape Ecology University of FreiburgTennenbacherstrasse 479106 FreiburgGermany Instituto Nacional de Tecnología Agropecuaria(INTA) Universidad Nacional de la Patagonia Austral(UNPA)Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET)Cc 332(9400)Río GallegosSanta CruzArgentina

Background It is necessary to determine the implications for managing forest stands using variable retention harvesting for maintaining carbon and for calculating the effects of different harvesting practices on above-and below-ground carbon balance in forest *** this context,forest carbon management has gained more attention among managers and policy-makers during recent *** aim of this study was to determine carbon pool dynamics in different forest ecosystem components after variable retention harvesting(VRH)to characterize the ecological stability and quantify the recovery rate through the years-after-harvesting(YAH).Methods Carbon pool compartmentalization of 14 different components was determined in 60 harvested and primary unmanaged forests during the first 18 YAH in Tierra del Fuego(Argentina).We compared them using uni-and multi-variate methods,relativizing the outputs with primary unmanaged *** We determined the effectiveness to retain carbon components in post-harvested stands under different retention strategies(aggregated ***).The balance among carbon pool components changed between managed and unmanaged stands across the YAH,and was directly related to the impact *** retention improved the ecological stability of the harvested areas,where the below-ground components were more stable than the above-ground *** recovery rate was directly related to the post-harvesting natural dynamics of the *** studied period was not enough to fully recover the C levels of primary unmanaged for-ests,but VRH showed advantages to increase the C pools in the managed *** Promoting VRH can improve sustainable forestry at the landscape level and in the long term,generating positive synergies with biodiversity and the provision of ecosystem *** study provides important new insights into forest carbon management,in particular to setting standards in carbon projects and sets the ground

关键词： Carbon reservoir Temperate forest Ecological stability Recovery rate Forest carbon management

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