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A Shortest-Path Tree Approach for Routing in Space Networks

China Communications 2020年第7期17卷 52-66页

作者： Olivier De Jonckère juan a.fraire Technische Universitat Dresden 01187Germany CONICET-Univerisidad Nacional de Córdoba 5000Argentina Saarland University Saarland Informatics CampusSaarbrückenGermany

A new space era is bringing deployments of thousands of networked assets in near-Earth and ***-Tolerant Networking(DTN)protocols and algorithms such as Contact Graph Routing(CGR)are state-of-the-art technologies certainly capable of achieving this ambitious ***,in this paper,we highlight and analyze the roots of current scalability limits of CGR,to then propose more efficient *** particular,we leverage a different graph modeling approach that enables optimizations such as priority queues and spanning-tree searches in the core route determination *** such abstract data types are difficult to adapt to CGR,we incorporate them into a novel Shortest-Path Tree Approach for Routing in Space Networks(SPSN),which is presented as a compute-efficient *** paper thoroughly describes the SPSN approach including route object modeling,capacity-oriented search,and potential route management *** evaluations analyzed in this paper present significant evidence that SPSN outperforms legacy CGR in more than an order of magnitude in contact plans comprising thousands of contacts.

关键词： bringing thoroughly enable

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Application of formal languages in polynomial transformations of instances between NP-complete problems

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Journal of Zhejiang University-Science C(Computers and Electronics) 2013年第8期14卷 623-633页

作者： Jorge A. RUIZ-VANOYE Joaquín PREZ-ORTEGA Rodolfo A. PAZOS RANGEL Ocotlán DíAZ-PARRA Héctor J. fraire-HUACUJA juan FRAUSTO-SOLíS Gerardo REYES-SALGADO Laura CRUZ-REYES DACI Universidad Autónoma del Carmen Computer Science Centro Nacional de Investigación y Desarrollo Tecnológico Systems and Computer Science Instituto Tecnológico de Ciudad Madero Informatics Universidad Politécnica del Estado de Morelos Computer Science Instituto Tecnológico de Cuautla

We propose the usage of formal languages for expressing instances of NP-complete problems for their application in polynomial transformations. The proposed approach, which consists of using formal language theory for polynomial transformations, is more robust, more practical, and faster to apply to real problems than the theory of polynomial transformations. In this paper we propose a methodology for transforming instances between NP-complete problems, which differs from Garey and Johnson's. Unlike most transformations which are used for proving that a problem is NP-complete based on the NP-completeness of another problem, the proposed approach is intended for extrapolating some known characteristics, phenomena, or behaviors from a problem A to another problem B. This extrapolation could be useful for predicting the performance of an algorithm for solving B based on its known performance for problem A, or for taking an algorithm that solves A and adapting it to solve B.

关键词： Formal languages Polynomial transformations NP-completeness

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Intracellular Delivery of mRNA in Adherent and Suspension Cells by Vapor Nanobubble Photoporation

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Nano-Micro Letters 2020年第12期12卷 404-420页

作者： Laurens Raes Stephan Stremersch juan Cfraire Toon Brans Glenn Goetgeluk Stijn De Munter Lien Van Hoecke Rein Verbeke Jelter Van Hoeck Ranhua Xiong Xavier Saelens Bart Vandekerckhove Stefaan De Smedt Koen Raemdonck Kevin Braeckmans Laboratory of General Biochemistry&Physical Pharmacy Ghent University9000 GhentBelgium Cancer Research Institute Ghent(CRIG) 9000 GhentBelgium Department of Diagnostic Sciences Ghent University9000 GhentBelgium VIB-UGent Center for Medical Biotechnology 9052 GhentBelgium Department of Biomedical Molecular Biology Ghent University9000 GhentBelgium Department of Biochemistry and Microbiology Ghent University9000 GhentBelgium

Efficient and safe cell engineering by transfection of nucleic acids remains one of the long-standing hurdles for fundamental biomedical research and many new therapeutic applications,such as CAR T cell-based *** has recently gained increasing attention as a more safe and versatile alternative tool over viral-or DNA transposon-based approaches for the generation of adoptive T ***,limitations associated with existing nonviral mRNA delivery approaches hamper progress on genetic engineering of these hard-to-transfect immune *** this study,we demonstrate that gold nanoparticle-mediated vapor nanobubble(VNB)photoporation is a promising upcoming physical transfection method capable of delivering mRNA in both adherent and suspension *** transfection experiments on HeLa cells showed the importance of transfection buffer and cargo concentration,while the technology was furthermore shown to be effective for mRNA delivery in Jurkat T cells with transfection efficiencies up to 45%.Importantly,compared to electroporation,which is the reference technology for nonviral transfection of T cells,a fivefold increase in the number of transfected viable Jurkat T cells was ***,our results point toward the use of VNB photoporation as a more gentle and efficient technology for intracellular mRNA delivery in adherent and suspension cells,with promising potential for the future engineering of cells in therapeutic and fundamental research applications.

关键词： Transfection mRNA Photoporation Optoporation Gold nanoparticles Vapor nanobubbles

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Repeated photoporation with graphene quantum dots enables homogeneous labeling of live cells with extrinsic markers for fluorescence microscopy

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Light(Science & Applications) 2018年第1期7卷 583-592页

作者： Jing Liu Ranhua Xiong Toon Brans Saskia Lippens Eef Parthoens Francesca Cella Zanacchi Raffaella Magrassi Santosh K.Singh Sreekumar Kurungot Sabine Szunerits Hannelore Bové Marcel Ameloot juan C.fraire Eline Teirlinck Sangram Keshari Samal Riet De Rycke Gaëlle Houthaeve Stefaan C.De Smedt Rabah Boukherroub Kevin Braeckmans Laboratory of General Biochemistry and Physical Pharmacy Faculty of PharmacyGhent UniversityGhent B-9000Belgium Centre for Nano-and Biophotonics Ghent UniversityGhent B-9000Belgium VIB-UGent Centre for Inflammation Research VIBGhent B-9000Belgium VIB Bioimaging Core VIBGhent B-9000Belgium Department of Biomedical Molecular Biology Ghent UniversityGhent B-9000Belgium Nanophysics(NAPH) Istituto Italiano di TecnologiaGenova 16163Italy Biophysics Institute(IBF) National Research Council(CNR)Via De Marini6-16149–GE GenovaItaly Physical and Materials Chemistry Division CSIR-National Chemical LaboratoryDr.Homi Bhabha RoadPune 411008India Academy of Scientific and Innovative Research Anusandhan Bhawan2 RafiMargNew Delhi 110001India Univ.Lille CNRSCentrale LilleISENUniv.ValenciennesUMR 8520-IEMNLille F-59000France Biomedical Research Institute Hasselt UniversityAgoralaan Building CDiepenbeek 3590Belgium Centre for Surface Chemistry and Catalysis KU LeuvenCelestijnenlaan 200FLeuven 3001Belgium Inflammation Research Center Image Core FacilityVIB9052 GhentBelgium Department of Biomedical Molecular Biology Ghent University9052 GhentBelgium Department of Plant Biotechnology and Bioinformatics Ghent University9052 GentBelgium Univ Lille 1 Univ Lille Nord FranceLab Phys Lasers Atomes&MolVilleneuve Dascq UMR 852359655France College of Chemical Engineering Jiangsu Key Lab of Biomass-based Green Fuels and ChemicalsNanjing Forestry University(NFU)Nanjing 210037China UMR 8523 Laboratoire de Physique des LasersAtomes et MoléculesUniversitéde LilleVilleneuve d’AscqFrance IEMN UMR 8520Universitéde LilleVilleneuve d’AscqFrance

In the replacement of genetic probes,there is increasing interest in labeling living cells with high-quality extrinsic labels,which avoid over-expression artifacts and are available in a wide spectral *** calls for a broadly applicable technology that can deliver such labels unambiguously to the cytosol of living ***,we demonstrate that nanoparticle-sensitized photoporation can be used to this end as an emerging intracellular delivery *** replace the traditionally used gold nanoparticles with graphene nanoparticles as photothermal sensitizers to permeabilize the cell membrane upon laser *** demonstrate that the enhanced thermal stability of graphene quantum dots allows the formation of multiple vapor nanobubbles upon irradiation with short laser pulses,allowing the delivery of a variety of extrinsic cell labels efficiently and homogeneously into live *** demonstrate high-quality time-lapse imaging with confocal,total internal reflection fluorescence(TIRF),and Airyscan superresolution *** the entire procedure is readily compatible with fluorescence(super resolution)microscopy,photoporation with graphene quantum dots has the potential to become the long-awaited generic platform for controlled intracellular delivery of fluorescent labels for live-cell imaging.

关键词： quantum enable replace

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