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New approach to assess sperm DNA fragmentation dynamics： Fine-tuning mathematical models

Journal of Animal Science and Biotechnology 2017年第3期8卷 592-600页

作者： Isabel Ortiz Jesus Dorado Jane morrell Jaime Gosalvez Francisco Crespo juan m.jimenez manuel Hidalgo Veterinary Reproduction Group Department of Animal Medicine and Surgery Faculty of Veterinary Medicine University of Cordoba 14071 Cordoba Spain Department of Clinical Sciences Division of Reproduction Swedish University of Agricultural Sciences Box 7054 SE-75007 Uppsala Sweden Department of Biology Universidad Aut6noma de Madrid 28049 Madrid Spain Department of Reproduction Centro Militar de Cria Caballar (FESCCR-Ministry of Defense) 05005 Avila Spain Department of Chemistry and Physics of materials University of Salzburg Hellbrunner StraBe 34/111 A-5020 Salzburg Austria

Background： Sperm DNA fragmentation（sDF） has been proved to be an important parameter in order to predict in vitro the potential fertility of a semen sample. Colloid centrifugation could be a suitable technique to select those donkey sperm more resistant to DNA fragmentation after thawing. Previous studies have shown that to elucidate the latent damage of the DNA molecule, sDF should be assessed dynamically, where the rate of fragmentation between treatments indicates how resistant the DNA is to iatrogenic damage. The rate of fragmentation is calculated using the slope of a linear regression equation. However, it has not been studied if s DF dynamics fit this model. The objectives of this study were to evaluate the effect of different after-thawing centrifugation protocols on sperm DNA fragmentation and elucidate the most accurate mathematical model（linear regression, exponential or polynomial） for DNA fragmentation over time in frozen-thawed donkey ***： After submitting post-thaw semen samples to no centrifugation（UDC）, sperm washing（SW） or single layer centrifugation（SLC） protocols, sD F values after 6 h of incubation were significantly lower in SLC samples than in SW or *** of determination（R-2） values were significantly higher for a second order polynomial model than for linear or exponential. The highest values for acceleration of fragmentation（aSDF） were obtained for SW, fol owed by SLC and ***： SLC after thawing seems to preserve longer DNA longevity in comparison to UDC and SW. moreover,the fine-tuning of models has shown that sDF dynamics in frozen-thawed donkey semen fit a second order polynomial model, which implies that fragmentation rate is not constant and fragmentation acceleration must be taken into account to elucidate hidden damage in the DNA molecule.

关键词： Colloid centrifugation Dynamics Fine-tuning mathematical models Sperm DNA fragmentation

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Safety of intravitreal quinupristin/dalfopristin in an animal model

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International Journal of Ophthalmology(English edition) 2016年第3期9卷 373-378页

作者： Veronica E.Giordano Sergio E.Hernandez-Da mota Tania N.A dabache-Guel Armando Castillejos-Cheve Sonia Corredor-Casas Samantha m.Salinas-Longoria Rafael Romero-Vera juan m.jimenez-Sierra Jose L.Guerrero-Naranjo Virgilio morales-Canton Department of Ophthalmology Retina and Vitreous Service Asociacion para Evitar la Ceguera en Mexico Vicente Garcia Torres 46 Coyoacan Barrio San Lucas Mexico City 04030 Mexico Ophthalmology Service Clinica David Boulevard Garcia de Leon 598 Nueva Chapultepec Morelia Michoacan 58280Mexico Department of Ophthalmology Glaucoma Service Asociacion para Evitar la Ceguera en Mexico Vicente Garcia Torres 46 Coyoacan Barrio San Lucas Mexico City 04030 Mexico Department of Ophthalmology Pathology Service Asociacion para Evitar la Ceguera en Mexico Vicente Garcia Tortes 46 Coyoacan Barrio San Lucas Mexico City 04030 Mexico

AIm： To determine whether different intravitreal doses of quinupristin/dalfopristin lead to electroretinographic or histological changes in the rabbit retina over one month period after injection. mETHODS： Eighteen New Zealand white rabbits were divided into three treatment groups （groups 1 to 3） and different intravitreal doses of quinupristin/dalfopristin were tested in each group. The right eye was injected with the drug and the left eye received intravitreal injection of 5% dextrose water and served as control eye. The doses delivered to each group were 0.1 mg/0.1 mL, 1 mg/0.1 mL and 10 mg/0.1 mL. Simultaneous, bilateral, dark-adapted electroretinography and clinical images of both eyes were obtained in all groups before injection （baseline） and after 7, 14, 21 and 28d, followed by enucleation for histological examination. RESULTS： Subjects in the group 1 showed no signs of toxicity in the electroretinogram when compared with groups 2 and 3 （Kruskall-Wallis test, P=0.000）. By day 7, no electrical response to light stimuli was recorded in the treated eyes in groups 2 and 3, consistent with severe damage due to retinal toxicity. Light microscopy revealedno significant histopathological changes in the group 1, while rabbits in groups 2 and 3 had signs of granulomatous inflammation in most cases. CONCLUSION： Intravitreal 0.1 mg/0.1 mL doses of quinupristin/dalfopristin do not lead to electroretinographic or histological signs of retinal toxicity compared with 1 mg/0.1 mL and 10 mg/O.1 mL in this rabbit model.

关键词： endophthalmitis quinupristin/dalfopristin retinal toxicity

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Salsolinol as an RNA m~6A methylation inducer mediates dopaminergic neuronal death by regulating YAP1 and autophagy

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Neural Regeneration Research 2025年第3期20卷 887-899页

作者： Jianan Wang Yuanyuan Ran Zihan Li Tianyuan Zhao Fangfang Zhang juan Wang Zongjian Liu Xuechai Chen Beijing International Science and Technology Cooperation Base for Antiviral Drugs College of Chemistry and LifeBeijing University of TechnologyBeijingChina Department of Rehabilitation Beijing Rehabilitation HospitalCapital Medical UniversityBeijingChina

Salsolinol(1-methyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline,Sal)is a catechol isoquinoline that causes neurotoxicity and shares structural similarity with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine,an environmental toxin that causes Parkinson's ***,the mechanism by which Sal mediates dopaminergic neuronal death remains *** this study,we found that Sal significantly enhanced the global level of N~6-methyladenosine(m~6A)RNA methylation in PC12 cells,mainly by inducing the downregulation of the expression of m~6A demethylases fat mass and obesity-associated protein(FTO)and alk B homolog 5(ALKBH5).RNA sequencing analysis showed that Sal downregulated the Hippo signaling *** m~6A reader YTH domain-containing family protein 2(YTHDF2)promoted the degradation of m~6A-containing Yes-associated protein 1(YAP1)mRNA,which is a downstream key effector in the Hippo signaling ***,downregulation of YAP1 promoted autophagy,indicating that the mutual regulation between YAP1 and autophagy can lead to *** findings reveal the role of Sal on m~6A RNA methylation and suggest that Sal may act as an RNA methylation inducer mediating dopaminergic neuronal death through YAP1 and *** results provide greater insights into the neurotoxic effects of catechol isoquinolines compared with other studies and may be a reference for assessing the involvement of RNA methylation in the pathogenesis of Parkinson's disease.

关键词： ALKBH5 autophagy FTO Hippo pathway m~6A Parkinson's disease RNA methylation salsolinol YAP1 YTHDF2

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A surface engineering strategy for the stabilization of zinc metal anodes with montmorillonite layers toward long-life rechargeable aqueous zinc ion batteries

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Journal of Energy Chemistry 2025年第1期100卷 94-105页

作者： Wenbo Wang Ruifeng Xu Xu Zhang Peiyu Wang Bao Yang Bingjun Yang juan Yang Kailimai Su Pengjun ma Yanan Deng Xianfeng Fan Wanjun Chen Key Laboratory for Electronic materials College of Electrical Engineering Northwest Minzu University Resource Chemistry and Energy materials Research Center State Key Laboratory of Solid Lubrication Lanzhou Institute of Chemical Physics Chinese Academy of Sciences University of Chinese Academy of Sciences Institute for materials and Processes School of Engineering University of Edinburgh

Rechargeable aqueous zinc-ion batteries （AZIBs） exhibit appreciable potential in the domain of electrochemical energy ***,there are serious challenges for AZIBs,for instance zinc dendrite growth,hydrogen evolution reaction （HER）,and corrosion side ***,we propose a surface engineering modification strategy for coating the montmorillonite （mmT） layer onto the surface of the Zn anode to tackle these issues,thereby achieving high cycling stability for rechargeable *** results reveal that the mmT layer on the surface of the Zn anode is able to provide ordered zincophilic channels for zinc ions migration,facilitating the reaction kinetics of zinc *** functional theory（DFT） calculations and water contact angle （CA） tests prove that mmT@Zn anode exhibits superior adsorption capacity for Zn2+and better hydrophobicity than the bare Zn anode,thereby achieving excellent cycling ***,the mmT@Zn||mmT@Zn symmetric cell holds the stable cycling over 5600 h at 0.5 mA cm-2and 0.125 m A h cm-2,even exceeding 1800 h long cycling under harsh conditions of 5 m A cm-2and 1.25 m A h *** mmT@Zn||V2O5full cell reaches over 3000 cycles at 2 A g-1with excellent rate ***,this surface engineering modification strategy for enhancing the electrochemical performance of AZIBs represents a promising application.

关键词： Surface engineering strategy montmorillonite layer Protection mechanism of Zn anode Cycling stability Aqueous zinc ion batteries

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Enhancing output performance of triboelectric nanogenerator by increasing charge storage capacity of electrodes

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Nano Research 2025年第1期18卷 395-403页

作者： Congcong Hao Zekun Wang mingzhe Cai Shuaining Cheng Zhongxin Wang Cong Zhai juan Cui Yongqiu Zheng Chenyang Xue Key Laboratory of Instrumentation Science and Dynamic measurement ministry of Education North University of ChinaTaiyuan 030051China

ABSTRACT:The practical application of rotating triboelectric nanogenerators is often limited by the wear of high-friction surface materials and low surface charge *** addition to the charge pump replenishment strategy,suppressing charge decay is also crucial for increasing surface charge ***,we present a high performance rotary triboelectric nanogenerator(HPR-TENG)based on a coplanar charge pumping strategy and polyvinyl chloride(PVC)*** has been demonstrated that applying PVC film to the surface of the storage electrode of the main TENG(m-TENG)significantly enhances the m-TENG’s output ***,the HPR-TENG with three layers of PVC film pasted achieved the best output performance,with a peak-topeak output voltage of 2828 V,a peak-to-peak output current of 327μA and a charge transfer of 0.81μC at 500 *** addition,the output improvement effects of different materials are *** TENG with 3 layers of PVC film pasted on it has a maximum output power of 748 mW at a load resistance of 4×10^(6)Ω.HPR-TENG’s output performance remains consistent after 100,000 cycles,which shows excellent *** excellent electrical performance of the HPR-TENG can be used as the energy supply for the tip high-voltage breakdown sensor system,which can achieve 14 breakdowns in 10 *** to its extraordinary electrical performance,HPRTENG can not only serve as an energy supply for cutting-edge high-voltage breakdown sensor systems,but also has the potential to serve as an energy supply for high-pressure sterilization,high-pressure vacuum and water electrolysis.

关键词： triboelectric nanogenerator coplanar charge pump enhancing charge density suppressing charge decay polyvinyl chloride(PVC)film

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Laser direct writing of flexible multifunctional airflow sensors on the Kevlar fabric

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Nano Research 2025年第1期18卷 443-454页

作者： Wei Wang Zi-Qing Chen Yong-Qi Li Yu-Long Wang mei-Chen Liu Yun-Bo Ruan Yang Zhang Shu-juan Liu Qiang Zhao College of Electronic and Optical Engineering and College of Flexible Electronics(Future Technology) Nanjing University of Posts and TelecommunicationsNanjing 210023China State Key Laboratory of Organic Electronics and Information Displays and Jiangsu Key Laboratory for Biosensors Institute of Advanced Materials(IAM)Nanjing University of Posts and TelecommunicationsNanjing 210023China Zhe Jiang Topsun Logistic Control CO. LTD.Yuhuan 317600China Department of Rehabilitation medicine School of Acupuncture-Moxibustion and Tuina and School of Health Preservation and RehabilitationNanjing University of Chinese MedicineNanjing 210023China

The growing interest in flexible devices has emerged as a global trend due to their advantages in flexibility,lightweight structure,and wearability,addressing the limitations of traditional *** wearable airflow sensors have been previously reported,the development of flexible fabric-based airflow sensors capable of functioning in environments with open flames—critical for fire rescue operations—has yet to be explored,largely due to the poor fire resistance of conventional *** this work,we first present a flexible,wearable,and multifunctional airflow sensor with excellent fire-resistant properties,fabricated through a simple direct laser writing *** sensor maintains airflow detection capabilities even in the presence of open ***,the fabrication of fabric-based sensors involves complex procedures such as carbon materials doping or vapor-phase deposition,leading to lengthy preparation cycles and high ***,fabric-based devices are inherently prone to *** address these challenges,we introduce twice-vertical laser-induced graphene(TVLIG)as a sensitive and reliable component for fire-resistant airflow *** resulting TVLIG/Kevlar fabric can be integrated into various garments,particularly protective suits,to form sensitive and fire-resistant airflow sensors capable of detecting airflow velocity and direction in both two-dimensional(2D)and three-dimensional(3D)spaces during fire ***,the TVLIG patterns can be expanded to multifunctional platforms,such as glucose detection for injured individuals,offering further applications in rescue *** functional expansion reduces the burden on rescue personnel and streamlines device *** its outstanding sensing capabilities,fire resistance,and expandability,the developed flexible airflow sensor shows great potential for various real-world rescue scenarios,promising advancements in wearable sensing technology for rescue enginee

关键词： flexible airflow sensor direct laser writing twice-vertical laser-induced graphene fire accident rescue Kevlar fabric

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A cloud-based platform to predict wind pressure coefficients on buildings

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Building Simulation 2022年第8期15卷 1507-1525页

作者： Facundo Bre juan m.Gimenez Centro de Investigacion de metodos Computacionales(CImEC) UNLCONICETPredio"Dr.Alberto Cassano"Colectora Ruta Nacional 168 s/n3000Santa FeArgentina

Natural ventilation(NV)is a key passive strategy to design energy-efficient buildings and improve indoor air ***,accurate modeling of the NV effects is a basic requirement to include this technique during the building design ***,there is an important lack of wind pressure coefficients(CP)data,essential input parameters for NV *** this,there are no simple but still reliable tools to predict CP data on buildings with arbitrary shapes and surrounding conditions,which means a significant limitation to NV modeling in real *** this reason,the present contribution proposes a novel cloud-based platform to predict wind pressure coefficients on *** platform comprises a set of tools for performing fully unattended computational fluid dynamics(CFD)simulations of the atmospheric boundary layer and getting reliable CP data for actual ***-expert decisions throughout the entire workflow are implemented to automatize the generation of the computational domain,the meshing procedure the solution stage,and the post-processing of the *** evaluate the performance of the platform,an exhaustive validation against wind tunnel experimental data is carried out for a wide range of case *** include buildings with openings,balconies,irregular floor-plans,and surrounding urban *** C_(P) results are in close agreement with experimental data,reducing 60%-77% the prediction error on the openings regarding the EnergyPlus *** platform introduced shows being a reliable and practical C_(P) data source for NV modeling in real building design scenarios.

关键词： natural ventilation building simulation airflow network model EnergyPlus wind pressure coefficient computational fluid dynamics

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Rehabilitation of degraded areas in northeastern Patagonia, Argentina: Effects of environmental conditions and plant functional traits on performance of native woody species

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Journal of Arid Land 2020年第4期12卷 653-665页

作者： juan m ZEBERIO Carolina A PÉREZ National University of Río Negro Atlantic HeadquartersCenter for Environmental Studies from Norpatagonia(CEANPa)Viedma 8500Argentina National Council of Scientific and Technical Research(CONICET) Viedma 8500Argentina Ecological and Environmental Systems Research Laboratory(LISEA) National University of La PlataLa Plata 1900Argentina

Degradation processes affect a vast area of arid and semi-arid lands around the world and damage the environment and people′s health. Degradation processes are driven by human productive activities that cause direct and indirect effects on natural resources, such as species extinction at regional scale, reduction and elimination of vegetation cover, soil erosion, etc. In this context, ecological rehabilitation is an important tool to recover key aspects of the degraded ecosystem. Rehabilitation trials rely on the use of native plant species with characteristics that allow them to obtain high survival and growth rates. The aim of this work was to assess the survival and growth of native woody species in degraded areas of northeastern Patagonia and relate them to plant functional traits and environmental variables. We observed high early and late survival rates, and growth rates in Prosopis flexuosa DC. var. depressa F.A. Roig and Schinus johnstonii F.A. Barkley, and low values in Condalia microphylla Cav. and Geoffroea decorticans(Gillies ex Hook. & Arn.) Burkart. Early survival rates were positively associated with specific leaf area(SLA) and precipitation, but negatively associated with wood density, the maximum mean temperature of the warmest month and the minimum mean temperature of the coldest month. Late survival rates were positively associated with SLA and soil organic matter, but negatively associated with plant height and precipitation. The temperature had a positive effect on late survival rates once the plants overcame the critical period of the first summer after they were transplanted to the field. Prosopis flexuosa and S. johnstonii were the most successful species in our study. This could be due to their functional traits that allow these species to acclimatize to the local environment. Further research should focus on C. microphylla and G. decorticans to determine how they relate to productive conditions, acclimation to environmental stress, auto-eco

关键词： arid lands Condalia microphylla Geoffroea decorticans Prosopis flexuosa Schinus johnstonii survival rates height growth basal diameter growth

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Sequence-activated chemiluminescence probes: programmable illumination for high-contrast in vivo sensing and imaging

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Science China Chemistry 2025年第1期 26-34页

作者： Yutao Zhang juan Li Wei-Hong Zhu Zhiqian Guo Shanghai Key Laboratory of Functional materials Chemistry Joint International Research Laboratory of Precision Chemistry and Molecular Engineering Institute of Fine Chemicals School of Chemistry and Molecular Engineering East China University of Science and Technology State Key Laboratory of Bioreactor Engineering Shanghai Frontier Science Research Base of Optogenetic Techniques for Cell MetabolismEast China University of Science & Technology

Sequence-activated chemiluminescence(CL) technique offers a unique approach to detecting specific biomolecules or cellular events with high sensitivity and spatial resolution. The fundamental principle behind sequence-activated CL probes involves tailoring highly-controllable CL signals in response to specific sequences or events of interest, thus enabling high-contrast signals under complex physiological conditions. This sequential activation could be triggered by enzymes, reactive oxygen species(ROS), biothiols or changes in the microenvironment. These unique sequence-activated CL probes bestow several advantages, including minimal background, deep-tissue penetration, and the ability of non-invasive or minimally invasive detection. The sequence-activated CL techniques represent a significant advancement in molecular imaging and diagnostics. In this mini-review, we focus on the latest developments in sequential CL probes, addressing the technical challenges and discussing the future trajectory by harnessing CL signaling and molecular recognition.

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Real-time hybrid simulation for structural control performance assessment

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Earthquake Engineering and Engineering Vibration 2009年第4期8卷 481-492页

作者： juan E Carrion BF Spencer Jr Brian m Phillips Skidmore Owings & Merrill LLP Civil and Environmental Engineering Department University of Illinois

Real-time hybrid simulation is an attractive method to evaluate the response of structures under earthquake loads. The method is a variation of the pseudodynamic testing technique in which the experiment is executed in real time, thus allowing investigation of structural systems with rate-dependent components. Real-time hybrid simulation is challenging because it requires performance of all calculations, application of displacements, and acquisition of measured forces, within a very small increment of time. Furthermore, unless appropriate compensation for actuator dynamics is implemented, stability problems are likely to occur during the experiment. This paper presents an approach for real-time hybrid simulation in which compensation for actuator dynamics is implemented using a model-based feedforward compensator. The method is used to evaluate the response of a semi-active control of a structure employing an mR damper. Experimental results show good agreement with the predicted responses, demonstrating the effectiveness of the method for structural control performance assessment.

关键词： real-time hybrid simulation mR damper semi-active control actuator dynamics

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