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2018—2020年多中心耐碳青霉烯类肠杆菌目细菌的流行病学特征及耐药性分析

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中国抗生素杂志 2021年第11期46卷 1038-1043页

作者：员静单斌孟雪斐张鸿娟马志刚喻华耿荣华曲芬刘平娟刘家云谢小芳蓝锴冀旭峰贾伟李刚鲍春梅季萍乌鲁木齐市友谊医院乌鲁木齐830049 昆明医科大学第一附属医院云南省医学检验临床医学研究中心昆明650032 四川省人民医院成都610072 航空总医院北京100012 中山大学附属第一医院广州510080 空军军医大学第一附属医院西安710032 苏州大学附属第二医院苏州215004 广东省中医院广州510120 吉林大学第一医院长春130000 宁夏医科大学总医院银川750004 解放军总医院第五医学中心北京100039 新疆医科大学第一附属医院乌鲁木齐830054

目的了解多中心耐碳青霉烯类肠杆菌目细菌的流行病学特征,为临床抗感染治疗提供依据。方法收集2018年1月—2020年12月全国不同地区11家医院6123株耐碳青霉烯类肠杆菌目细菌(carbapenem-resistant Enterobacterales,CRE)临床分离株,按统... 详细信息

目的了解多中心耐碳青霉烯类肠杆菌目细菌的流行病学特征,为临床抗感染治疗提供依据。方法收集2018年1月—2020年12月全国不同地区11家医院6123株耐碳青霉烯类肠杆菌目细菌(carbapenem-resistant Enterobacterales,CRE)临床分离株,按统一方案和标准进行菌株鉴定和体外药物敏感性试验,药敏结果参照2020版CLSI标准判读,并用WHONET 5.6软件统计分析。结果3年间CRE检出率分别是6.4%(1897/29525)、6.8%(2186/32239)、7.3%(2171/29816),其中耐碳青霉烯类肺炎克雷伯菌(CR-KPN)、大肠埃希菌(CR-ECO)的3年总检出率分别是13.2%和2.0%。CRE菌株主要分离自痰液标本43.8%(2681/6123),其次是尿液标本16.0%(977/6123)、血液标本8.1%(496/6123)和分泌物标本4.5%(278/6123)。不同种属耐碳青霉烯类肠杆菌目细菌中不同标本类型的占比不同,CR-KPN以痰液标本为主,CR-ECO以尿液标本为主。不同科室的CRE检出率有所不同,以ICU检出最高。体外药敏结果显示,CRE对临床常用抗菌药物高度耐药,不同菌属CRE对临床常用抗菌药物的耐药率略有不同。CR-KPN和CP-ECO对大多数抗菌药物的耐药率高于耐碳青霉烯类阴沟肠杆菌(CR-ECL),对替加环素、多黏菌素B的体外抗菌活性较高。结论CRE检出呈上升趋势,各地区需加强本地区CRE监测,加强院内感染防控措施,有效遏制CRE的暴发流行。

关键词：耐碳青霉烯类肠杆菌目细菌细菌耐药性监测耐碳青霉烯类肺炎克雷伯菌

Isolation, Identification, and Herbicidal Activity of Metabolites Produced by Pseudomonas aeruginosa CB-4

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Journal of Integrative Agriculture 2014年第8期13卷 1719-1726页

作者： YANG juan CAO hong-zhe WANG Wei ZHANG Li-hui DONG Jin-gao Mycotoxin and Molecular Plant Pathology Laboratory Agricultural University of Hebei

CB-4, a bacterial strain with highly effective herbicidal activity, was isolated from infected corn leaves. Through morphology, physiological and biochemical tests, and 16 S ribosomal DNA gene sequencing methods, CB-4 was identified as Pseudomonas aeruginosa. We conducted activity-evaluation experiments in the laboratory to assess the herbicidal potential of metabolites produced by strain CB-4. Crude extracts of strain CB-4 have high inhibition activity on Digitaria sanguinalis. In general, the root and shoot growth parameters of D. sanguinalis were significantly reduced by metabolites of strain CB-4. The IC50 of the culture filtrate extracts for the radicula and coleoptile of D. sanguinalis were 0.299 and 0.210 mg mL-1, respectively. Component 2 of the herbicidal activity of the crude toxin from strain CB-4 was successfully purified for the first time by using high-speed counter current chromatography with a two-phase solvent system composed of petroleum ether-ethyl acetate-methanol-water（4：5：4：5, v/v） and high-performance liquid chromatography. We concluded that the metabolites of strain CB-4 have the potential to be developed as a microbe-based herbicide.

关键词： Pseudomonas aeruginosa bioherbicide biocontrol HSCCC HPLC

Major physiological adjustments in freezing-tolerant grey tiger longicorn beetle(Xylotrechus rusticus) during overwintering period

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Journal of Forestry Research 2014年第3期25卷 653-659页

作者： Jue-wen Li juan Shi Yu Xue hong-bo Mao You-qing Luo Beijing Key Laboratory of Forest Pest Control Beijing Forestry University College of Forestry Northeast Forestry University Forestry Administration of Acheng District

1Xylotrechus rusticus （Linnaeus） is one of the most destruc-tive woodborers in northeast China;it damages poplar and is listed as a domestic forestry quarantine pest. Overwintering larvae were collected during October 2012 and March 2013 in Harbin, China, to quantify indi-cators related to the insect’s overwintering strategy and the major cryo-protectants. The supercooling points （SCPs）, which ranged from-14.7＆#176;C to -2.9＆#176;C, were higher than the lethal temperatures of LT50 （-33.64＆#176;C） and LT99 （-40.17＆#176;C） after 24 h exposure. , also the minimum mean daily temperature （-24.5＆#176;C） and mean monthly temperature （-18.0＆#176;C） at the sampling site in January during 2008-2012. Thus, X. rusticus is a typical freezing-tolerant insect. Glycerol serves as a major cryoprotectant for overwintering larvae , because it was the only polyol accumulated during the winter and it also had a significant negative correlation with the SCP （p=0.033, R=0.907）. The glycogen and lipid are major sources of ener-gy and their levels decreased substantially in the middle of overwintering, when glycogen had a significant correlation with the SCP （p= 0.006, R=0.971） whereas the lipid contents did not. Moreover, inter-conversions between glycerol and glycogen, as well as mannose and glycogen, were suggested by their negative correlations. The water content did not change obviously during the winter and was not correlated with the SCP. The free amino acids in the hemolymph and the total protein contents of the bodies of larvae changed significantly during winter, although both had no correlations with the SCP.

关键词： cold hardiness cryoprotectant freezing tolerant physiological variation Xylotrechus rusticus.

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木质素改性制备驱油剂的研究进展

现代化工 2008年第S2期28卷 252-254,256页

作者：崔凯周玉杰张建安程可可刘宏娟戴玲妹刘德华王永刚中国矿业大学(北京)化学与环境工程学院北京100083 清华大学核能与新能源技术研究院北京100084 清华大学化学工程系北京100084

在石油资源日趋减少的情况下,使用驱油剂已成为一种重要的三次采油方法。木质素来源丰富,经过改性可用作驱油剂,从而减少由于不合理使用木质素造成的浪费,同时降低驱油剂的生产成本。本文综述了磺化、氧化、烷基化、胺化、接枝共聚、缩... 详细信息

在石油资源日趋减少的情况下,使用驱油剂已成为一种重要的三次采油方法。木质素来源丰富,经过改性可用作驱油剂,从而减少由于不合理使用木质素造成的浪费,同时降低驱油剂的生产成本。本文综述了磺化、氧化、烷基化、胺化、接枝共聚、缩合等化学改性方法合成木质素基驱油剂的研究现状及其应用进展。

关键词：木质素化学改性驱油剂

Sub-Lethal Effects of Fenvalerate on the Development, Fecundity, and Juvenile Hormone Esterase Activity of Diamondback Moth, Plutella xylostella (L.)

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Agricultural Sciences in China 2010年第11期9卷 1612-1622页

作者： WEI Hui WANG juan LI hong-shan DAI Hua-guo GU Xiao-jun institute of Plant Protection Fujian Academy of Agricultural Sciences Fuzhou 350013 P.R.China College of Plant Protection Nanjing Agricultural University Nanjing 210095 P.R. China Yancheng Institute of Technology Yancheng 224003 P.R.China Institute of Applied Ecology Fujian Agriculture and Forestry University Fuzhou 350002 P.R.China

The toxicities of fenvalerate （20% EC） to the 3rd instar larvae of diamondback moth （DBM）, Plutella xylostella （L.）, reared on three host plants viz., radish, oilseed rape, and cabbage were tested. The LC50 values of fenvalerate to the 3rd instar larvae of DBM varied with host plants, however, there wasn＇t any significant difference among them （P〉 0.05）. Similarly, DBM fed on three host plants had same pupal weight, pupal period, pupation rate, adult emergence rate, female ratio, and fecundity. The activity of juvenile hormone esterase （JHE, EC 3.***.1.1） in the 3rd instar larvae of DMB did not significantly vary with host plants, either. These suggested that DBM had similar fitness on the three host plant species. When fed on the host plants pretreated with fenvalerate at the concentrations equivalent to LC20, LC50 and LC50, the pupation rate, pupal weight, adult emergence rate, female ratio, fecundity, and JHE activity of the tested insects were declined as compared with insects in control treatments fed on the same host plant species. Furthermore, the pupal period of the tested insects was extended after fenvalerate treatment. The decrease in JHE activity after fenvalerate treatment in the tested insects could partly explain the changes in the mentioned growth parameters. Whether the role of fenvalerate in the inhibition of JHE activity could serve as a new way to control DBM needs further investigation.

关键词： crucifer plants fenvalerate sublethal effect juvenile hormone esterase (JHE) diamondback moth (DBM),Plutella xylostella (L.)

Preparation and property of a novel soluble electron transport POSS-based hybrid material

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Chinese Chemical Letters 2008年第6期19卷 634-638页

作者： Xin Wang juan Shen Jin Cui Wu Min Fang hong Yao Xu School of Chemistry and Chemical Engineering Anhui University Hefei 230039 China College of Material Science and Engineering ＆ State Key Laboratory of Chemical Fibers and Polymeric Materials Donghua University Shanghai 201620 China School of Chemistry Chizhou University Chizhou 247000 China

A novel POSS-based organic/inorganic hybrid covalently attached at molecular level, 2-（4-（allyloxy）phenyl）-5-（4-（octyloxy）phenyl）-1,3,4-oxadiazole-POSS （6）（abbreviated as oxadiazole-POSS） was synthesized by Pt（dcp） catalyst. The hybrid was soluble in common organic solvents such as CHCl3, toluene, C2H4Cl2, and THF. Its structures and properties were characterized and evaluated with FTIR, 1^H NMR, 13^C NMR,29^Si NMR, EA, TGA, DSC, GPC, and CV, respectively. The results show that the novel hybrid possesses high thermal stability and good electron injection ability.

关键词： CV Electron transport Oxadiazole POSS Thermal stability

大豆苷元靶向MMP12发挥抗口腔鳞状细胞癌的作用

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局解手术学杂志 2023年第6期32卷 474-480页

作者：吕红娟张璇李立恒孟媛媛赵亭亭河北北方学院附属第一医院口腔颌面外科河北张家口075000

目的分析大豆苷元靶向MMP12发挥抗口腔鳞状细胞癌的作用。方法数据库分析大豆苷元靶点及其与口腔鳞状细胞癌病理特征的关系。将CAL-27和Tca8113细胞分为对照组、大豆苷元组、大豆苷元+MMP12组。MTT实验检测细胞存活率,CCK-8实验检测细... 详细信息

目的分析大豆苷元靶向MMP12发挥抗口腔鳞状细胞癌的作用。方法数据库分析大豆苷元靶点及其与口腔鳞状细胞癌病理特征的关系。将CAL-27和Tca8113细胞分为对照组、大豆苷元组、大豆苷元+MMP12组。MTT实验检测细胞存活率,CCK-8实验检测细胞增殖活性,克隆形成实验检测细胞生长情况,Transwell实验检测细胞迁移和侵袭情况,Western blot检测MMP12蛋白表达。裸鼠皮下注射人口腔鳞状细胞癌CAL-27细胞株构建皮下移植瘤模型,daidzein组每隔1 d注射大豆苷元1次,control组注射等量生理盐水。计算肿瘤体积和质量,免疫组化法检测移植瘤组织中Ki67蛋白表达。结果GEO2R分析显示,MMP12在口腔鳞状细胞癌中表达(P<0.05),且MMP12表达与淋巴结转移相关(P<0.05)。GSEA分析表明,与细胞增殖、迁移和侵袭相关的基因集均富集在MMP12高表达组。大豆苷元浓度越高,作用时间越长,口腔鳞状细胞癌细胞活性、克隆细胞数、细胞侵袭数和迁移数明显越低/少(P<0.05)。daidzein组移植瘤体积、质量、Ki67蛋白表达水平均明显小/低于control组(P<0.05)。随着大豆苷元作用时间延长或浓度升高,MMP12表达水平明显下降(P<0.05)。与对照组相比,大豆苷元组口腔鳞状细胞癌细胞MMP12表达水平、细胞活性、克隆细胞数、细胞迁移和侵袭数明显降低/减少(P<0.05);与大豆苷元组比较,大豆苷元+MMP12组口腔鳞状细胞癌细胞活性、克隆细胞数、细胞迁移和侵袭数明显升高/增加(P<0.05)。结论大豆苷元可通过靶向MMP12下调MMP12表达,进一步抑制口腔鳞状细胞癌细胞增殖、迁移和侵袭,从而发挥抗肿瘤作用。

关键词：大豆苷元口腔鳞状细胞癌细胞增殖细胞迁移侵袭 MMP12

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应用型人才培养导向下专业课程教学模式改革研究——以石家庄学院人文地理与城乡规划专业为例

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石家庄学院学报 2022年第3期24卷 134-139页

作者：郑红娟商哲张淑媛李晨静陆璐石家庄学院资源与环境科学学院河北石家庄050035

合理科学的专业课程教学模式是应用型人才培养的关键.为完善人文地理与城乡规划专业课程教学模式,通过问卷调查和与用人单位访谈的方式摸清现行教学模式存在的问题.结果显示存在以下问题:专业课程体系与行业发展脱离;专业课程教学课程... 详细信息

合理科学的专业课程教学模式是应用型人才培养的关键.为完善人文地理与城乡规划专业课程教学模式,通过问卷调查和与用人单位访谈的方式摸清现行教学模式存在的问题.结果显示存在以下问题:专业课程体系与行业发展脱离;专业课程教学课程内容理论性较强,理论学时比例偏高;专业课程教学方式传统化;考核方式精准度不高.针对这些问题,提出了改革措施:构建实用性强的专业课课程体系;加强实践教学;采用多元化的教学方式;采取多样化的考核方式.

关键词：应用型人才专业课程教学模式人文地理与城乡规划

Antifungal Activity of Chitosan on Fusarium sulphureum in Relation to Dry Rot of Potato Tuber

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Agricultural Sciences in China 2009年第5期8卷 597-604页

作者： LI Yong-cai SUN Xiao-juan BI Yang GE Yong-hong WANG Yi College of Food Science and Engineering Gansu Agricultural University Lanzhou 730070 P.R.China

The antifungal activity of chitosan on Fusarium sulphureum in relation to the inhibitory effect on dry rot of potato tuber was investigated. The results showed spore germination and mycelial growth of F. sulphureum were inhibited by chitosan treatment and the inhibitory effect was highly correlated with chitosan concentration used in this study. Morphological changes such as intertwisting hyphal, distortion, and swelling with excessive branching were observed by scanning electron microscopy （SEM） observation. Transmission electron microscopy （TEM） observation further indicated the ultrastructural alterations of hyphae. These changes included abnormal distribution of cytoplasma, non-membraneous inclusion bodies assembling in cytoplasm, considerable thickening of the hyphal cellular walls, and very frequent septation with malformed septa. Application of chitosan at higher concentration caused serious damage to fungal hyphae, including cellular membrane disorganisation, cell wall disruption, and breaking of inner cytoplast. New hyphae （daughter hyphae） inside the collapsed hyphal cells was often detected in the cytoplasm of chitosan-treated hyphae. In vivo tests showed that chitosan treatment at 0.5 or 1% effectively controlled the dry rot of potato tuber inoculated with a spore suspension ofF. sulphureum. However, the chitosan treatment at 1% caused phytotoxicity to potato tuber. This study suggests that the use of chitosan could be a promising handling as a natural fungicide to partially substitute for the synthetic fungicides in potato tuber.

关键词： chitosan Fusarium sulphureum morphology and ultrastructure potato dry rot