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Characterization of the Bombyx mori Cecropin A 1 promoter regulated by IMD pathway

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Insect Science 2016年第2期23卷 297-304页

作者： Xiao-Ting Hua Xiao-ju.n ma Ren-ju.Xue Ting-Cai Cheng Fei Wang Qing-You Xia State Key Laboratory of Silkworm Genome Biology Southwest University Chongqing 400716 China

Cecropin A1 （CeeA1） promoter from Bombyx mori was cloned and character- ized to provide insight into the transcriptional control of this antimicrobial peptide gene upon immune challenges. Reporter gene assays demonstrated that both Escherichia coli and lipopolysaccharide could induce expression in BmE cells but B. bombyseptieus or peptidoglycan failed, and the induction pattern of the reporter gene was coincident with the endogenous CecAl. Analysis of deletion and mutation constructs revealed that the regulatory region was the κB motif located between -176 and -166, and no other pre- dicted elements on CecAl promoter affected its inducibility. Insertion of additional κB motifs increased the activity of CecAl promoter. Furthermore, binding of Relish to lob motif was confirmed by electrophoretic mobility shift assay. These findings indicate the regulatory mechanism of CecAl expression in IMD pathway and suggest an approach of engineering antimicrobial peptide promoter with enhanced activities that may lead to broad applications.

关键词： Bombyx mori Cecropin A1 promoter immune challenges κB motif Relish

Dislocation configuration evolution during extension twinning and its influence on precipitation behavior in AZ80 wrought magnesium alloy

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Journal of magnesium and Alloys 2023年第7期11卷 2442-2457页

作者： Guo-Liang Shi Kui Zhang Xing-Gang Li Yong-ju. Li Ming-Long ma Jia-Wei Yuan Hong-ju.Zhang State Key Laboratory of Nonferrous Metals and Process Grinm Group Corporation Limited(GRINM)Beijing 100088China GRImaT Engineering Institute Co. Ltd.Beijing 101407China Guobiao(Beijing)Testing and Certification.Co. Ltd.Beijing 101407China

Thermomechanical treatment T10(extension twinning+aging treatment)can largely enhance the precipitation strengthening effect of magnesium *** this study,dislocation structure evolution and precipitation behavior during T10 treatment of an AZ80 extruded bar were analyzed mainly by two-beam diffraction in *** a compressive strain of 1%in the extrusion direction(ED),a typical dislocation configuration,including basal I1 stacking faults(SFs)anddislocations,has been established in extension *** the strain reaches 7%,the volume fraction of extension twins increases to more than 90%at which point high dense I1 SFs anddislocations *** aging for 2 h at 150℃for the 7%strained sample,masses of basal I1 SFs anddislocations remain in the extension twins and can act as effective nucleation sites and solute fast-diffusion channels for continuous ***,the precipitates in extension twins become highly dense.

关键词： magnesium alloy Extension twin Dislocation Stacking fault Precipitation

Fine mapping and genetic analysis of resistance genes,Rsc18,against soybean mosaic virus

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Journal of Integrative Agriculture 2022年第3期21卷 644-653页

作者： LIU Sang-lin CHENG Yan-bo ma Qi-bin LI Mu JIANG Ze XIA Qiu-ju.NIAN Hai The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources South China Agricultural UniversityGuangzhou 510642P.R.China The Key Laboratory of Plant Molecular Breeding of Guangdong Province College of AgricultureSouth China Agricultural UniversityGuangzhou 510642P.R.China maize Research Institute Jilin Academy of Agricultural SciencesGongzhuling 130033P.R.China Beijing Genomics Institute(BGI)-Shenzhen Shenzhen 518086P.R.China

Soybean mosaic virus(SMV) affects seed quality and production of soybean(Glycine max(L.) Merr.) ***18 is one of the dominant SMV strains in South China,and accession Zhonghuang 24 displayed resistance to *** F_(1),F_(2) and 168 F_(11) recombinant inbred lines(RILs) population derived from a hybridization between Zhonghuang 24(resistant,R) and Huaxia 3(susceptible,S) were used in this *** to the segregation ratios of the F_(2) generation(3 R:1 S) and the recombinant inbred lines(RILs) population(1 R:1 S),one dominant locus may regulate the resistance to SC18 in Zhonghuang *** using composite interval mapping(CIM),Rsc18 was mapped to a 415.357-kb region on chromosome *** candidate genes,including one NBS-LRR type gene and two serine/threonine protein type genes,were identified according to the genetic annotations,which may be related to the resistance to *** q RT-PCR demonstrated that these genes were up-regulated in the R genotype compared to the *** conclusion,the findings of this research enhanced the understanding about the R genes at the Rsc18 ***,our results will provide insights for designing molecular markers to improve marker-assisted selection and developing new varieties with resistance to SC18.

关键词： soybean mosaic virus(SMV) fine mapping recombinant inbred lines(RILs) resistance gene

Effect of Glucose-lowering of Heteropolytungstogermanate β-K_6H[GeW_9V_3O_(40)]·9H_2O

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Chemical Research in Chinese Universities 2002年第3期18卷 246-248页

作者： LIU Jing fu LIU ju.tao ma Jian fang CHEN Ya guang LI Cai Department of Chemistry Northeast Normal UniversityChangchun 130024P.R.China Institute of Basic Applied Medicine Jilin UniversityChangchun 130021P.R.China

Preliminary tests in rats have shown that the title complex is an efficient insulin mimic, which may be the first example of polyoxomatalates possessing insulin mimetic activity.

关键词： Heteropolytungstogermanate Insulin mimetic activity Effect of glucose lowering

Dynamical stability of dipolar condensate in a parametrically modulated one-dimensional optical lattice

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Chinese Physics B 2021年第6期30卷 143-148页

作者： Ji-Li ma Xiao-Xun Li Rui-Jin Cheng Ai-Xia Zhang ju.Kui Xue College of Physics and Electronics Engineering Northwest Normal UniversityLanzhou 730070China

We study the stabilization properties of dipolar Bose–Einstein condensate in a deep one-dimensional optical lattice with an additional external parametrically modulated harmonic trap potential. Through both analytical and numerical methods, we solve a dimensionless nonlocal nonlinear discrete Gross–Pitaevskii equation with both the short-range contact interaction and the long-range dipole–dipole interaction. It is shown that, the stability of dipolar condensate in modulated deep optical lattice can be controled by coupled effects of the contact interaction, the dipolar interaction and the external modulation. The system can be stabilized when the dipolar interaction, the contact interaction, the average strength of potential and the ratio of amplitude to frequency of the modulation satisfy a critical condition. In addition, the breather state, the diffused state and the attractive-interaction-induced-trapped state are predicted. The dipolar interaction and the external modulation of the lattice play important roles in stabilizing the condensate.

关键词： Bose–Einstein condensate optical lattice dipole–dipole interaction periodic modulation

Haze insights and mitigation in China: An overview

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Journal of Environmental Sciences 2014年第1期26卷 2-12页

作者： Xuliang Zhuang Yuesi Wang Hong He Jianguo Liu Xinming Wang Tingyu Zhu maofa Ge ju.Zhou Guiqian Tang Jinzhu ma Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Institute of Atmospheric Physics Chinese Academy of Sciences Anhui Institute of Optics and Fine Mechanics Hefei Institutes of Physical Science Chinese Academy of Sciences Guangzhou Institute of Geochemistry Chinese Academy of Sciences Institute of Process Engineering Chinese Academy of Sciences Institute of Chemistry Chinese Academy of Sciences Bureau of Science and Technology for Development Chinese Academy of Sciences

The present article provides an overview of the chemical and physical features of haze in China, focusing on the relationship between haze and atmospheric fine particles, and the formation mechanism of haze. It also summarizes several of control technologies and strategies to mitigate the occurrence of haze. The development of instruments and the analysis of measurements of ambient particles and precursor concentrations have provided important information about haze formation. Indeed, the use of new instruments has greatly facilitated current haze research in China. Examples of insightful results include the relationship between fine particles and haze, the chemical compositions and sources of particles, the impacts of the aging process on haze formation, and the application of technologies that control the formation of haze. Based on these results, two relevant issues need to be addressed： understanding the relationship between haze and fine particles and understanding how to control PM2.5.

关键词： haze secondary aerosols size distribution particles chemical composition

Changes in starch quality of mid-season indica rice varieties in the lower reaches of the Yangtze River in last 80 years

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Journal of Integrative Agriculture 2020年第12期19卷 2983-2996页

作者： ZHANG Hao JING Wen-jiang XU Jing-ju.ma Bing-ju.WANG Wei-lu ZHANG Wei-yang GU ju.-fei LIU Li-ju. WANG Zhi-qin YANG Jian-chang Jiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Key Laboratory of Crop Cultivation and Physiology/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops Yangzhou UniversityYangzhou 225009P.R.China

Rice (Oryza sativa L.) quality depends mainly on the characteristics of starch stored in kernels. Understanding the changes in starch characteristics in kernels during variety improvement would have great significance to improve rice quality. This study was designed to investigate the starch characteristics in the kernels and associated physiological traits of indica rice varieties in the lower reaches of the Yangtze River in China in last 80 years. Eight representative mid-season indica rice varieties were grown in the field. The results showed that the grain yield was significantly increased with the improvement of varieties and such an increase was mainly attributed to the increase in total number of spikelets. The tall varieties applied in the 1940s–1950s had higher protein content, relative crystallinity and infrared (IR) ratio of 1 045/1 022 cm^–1. The semi-dwarf varieties applied in the 1980s–1990s had higher gel consistency, amylopectin content, IR ratio of 1 022/995 cm^–1, and breakdown value. With the improvement of varieties, the amylose content, large-sized starch granule number and volume distribution, onset and peak of gelatinization temperature, gelatinization and retrogradation enthalpy, setback value, pasting temperature, viscosity of peak, hot and final, and 1-aminocycopropane-1-carboxylic acid (ACC) concentrations in panicles and root bleeding were gradually decreased, whereas the medium-sized starch granule number and volume distribution, activities of key enzymes in grains, and zeatin (Z)+zeatin riboside (ZR) contents in panicles and root bleeding at grain filling stage were gradually increased. Correlation analysis showed that starch thermodynamic characteristics were closely related to starch structure and components, key enzymes and hormones. The results suggest that starch quality was enhanced through the optimization of starch components, structure, thermodynamics, and the regulation of key enzymes in grains and hormones in panicles and roo

关键词： rice starch thermodynamics hormone variety improvement

High cell density and high expression of recombinant human ApoA-I_(Milano) in Escherichia coli by twice temperature-shifted induction

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Frontiers in Biology 2006年第4期1卷 345-348页

作者： ZHUANG Yingping ma Wenfeng GUO Meijin DING mansheng CHU ju.ZHANG Siliang State Key Laboratory of Bioreactor Engineering National Research Center for BiotechnologyEast China University of Science and TechnologyShanghai 200237China State Key Laboratory of Bioreactor Engineering and National Research Center for Biotechnology East China University of Science and TechnologyShanghai 200237China

The effect of temperature on the formation of recombinant protein,apolipoprotein A-IMilano was investigated in the present *** temperature of the initial growth phase was set at 30ºC,while temperature variation in induction phase was arranged in three *** cell-density culture of Escherichia coli and high expression of recombinant human by twice temperature-shifted induction were carried *** results showed that ApoA-IMilano reached 4.8 g/L with the final cell density of OD600,*** was found that twice temperature-shifted induction could successfully avoid the effect of acetic acid on cell density and the expression of the *** present study provides a basic procedure for the production of recombinant ApoA-IMilano.

关键词： recombinant Escherichia coli ApoA-IMilano high density high expression twice temperature-shifted induction

Accumulation and bioavailability of heavy metals in a soil-wheat/maize system with long-term sewage sludge amendments

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Journal of Integrative Agriculture 2018年第8期17卷 1861-1870页

作者： YANG Guo-hang ZHU Guang-yun LI He-lian HAN Xue-mei LI ju.mei ma Yi-bing School of Resources and Environment University of Jinan Institute of Agricultural Resources and Regional Planning Chinese Academy of Agricultural Sciences

A long-term field experiment was carried out with a wheat-maize rotation system to investigate the accumulation and bioavailability of heavy metals in a calcareous soil at different rates of sewage sludge amendment. There are significant linear correlations between the contents of Hg, Zn, Cu, Pb, and Cd in soil and sewage sludge amendment rates. By increasing 1 ton of applied sludge per hectare per year in soil, the contents of Hg, Zn, Cu, Pb, and Cd in soil increased by 6.20, 619, 92.9, 49.2, and 0.500 μg kg–1, respectively. For Hg, sewage sludge could be safely applied to the soil for 18 years at an application rate of 7.5 t ha–1 before content exceeded the soil environmental quality standards in China(1 mg kg–1). The safe application period for Zn is 51 years and is even longer for other heavy metals(112 years for Cu, 224 years for Cd, and 902 years for Pb) at an application rate of 7.5 t ha–1 sewage sludge. The contents of Zn and Ni in wheat grains and Zn, Cu, and Cr in maize grains increased linearly with increasing sewage sludge amendment rates. The contents of Zn, Cr, and Ni in wheat straws and Zn, Cu, and As in maize straws were positively correlated with sewage sludge amendment rates, while the content of Cu in wheat straws and Cr in maize straws showed the opposite trend. The bioconcentration factors of the heavy metals in wheat and maize grains were found to be in the order of Zn>Cu>Cd>Hg>Cr=Ni>Pb>As. Furthermore, the bioconcentration factors of heavy metals in wheat were greater than those in maize, indicating that wheat is more sensitive than maize as an indicator plant. These results will be helpful in developing the critical loads for sewage sludge amendment in calcareous soils.

关键词： sewage sludge agricultural use heavy metals calcareous soil bioconcentration factors