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Enhanced Resistance of Snowdrop Lectin （Galanthus nivalis L. agglutinin） Expressing Maize to Asian Corn Borer （Ostriniafurnacalis Guenée）

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Journal of Integrative Plant Biology 2005年第7期47卷 873-880页

作者： Zhao-YuWANG Xiao-FenSUN FeiWANG Ke-XuanTANG ju-renzhang SchoolofLifeSciences ShandongUniversityJinan250100China StateKeyLaboratoryofGeneticEngineering InstituteofGeneticsSchoolofLifeSciencesFudanUniversityShanghai200433China SchoolofAgricultureandBiology ShanghaiJiaoTongUniversityShanghai200030China

In order to enhance the resistance to pests, transgenic maize (Zea mays L.) plants from elite inbred lines containing the gene encoding snowdrop lectin (Galanthus nivalis L. agglutinin; GNA) under control of a phloem-specific promoter were generated through the Agrobacterium tumefaciens-mediated also studied. Thirty-six independently derived plants were subjected to molecular analyses. The level of GNA expression at 0.13%-0.28% of total soluble protein was observed in different transgenic plants. The progeny of three GNA-expressing independent transformants that were derived separately from the elite inbred lines DH4866, DH9942, and 8902, were selected for examination of resistance to ACB. These plants synthesized GNA at levels above 0.24% total soluble protein and enhanced resistance to ACB was demonstrated by exposing the plants to insects under greenhouse conditions. Semi-artificial diet bioassays also showed the toxic effect of GNA on ACB. Field evaluation of the transgenic plants supported the results from the artificial trial. In the present study, we have obtained new insect-resistant maize material for further breeding work and have found that GNA-expressing plants not only gained significant resistance to homopterans, but also showed toxicity to ACB, which is a type of Lepidoptera.

关键词： com borer Galanthus nivalis agglutinin gene(gna) maize resistance to pests transgene.

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