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Heat Stress Upregulates the Expression of TLR4 and Its Alternative Splicing Variant in Bama Miniature Pigs

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Journal of Integrative Agriculture 2014年第11期13卷 2479-2487页

作者： ju Xiang-hong XU Han-jin YONG Yan-hong AN Li-long XU Ying-mei JIAO Pei-rong LIAO Ming Department of Veterinary Medicine Guangdong Ocean University Key Laboratory of Animal Disease Control and Prevention Ministry of Agriculture/College of Veterinary MedicineSouth China Agricultural University Department of Animal Science Guangdong Ocean University

Alternative splicing is a cellular mechanism in eukaryotes that results in considerable diversity ofgene products. It plays an important role in several diseases and cellular signal regulation. Heat stress is a major factor that induces immunosuppression in pigs. Little is known about the correlation between alternative splicing and heat stress in pigs. Therefore, this study aimed to clone, sequence and quantify the alternative splicing variant of toll-like receptor 4 （TLR4） in Bama miniature pigs （Sus scrofa domestica） following exposure to heat stress. The results showed that the second exon of TLR4 was spliced and 167 bp shorter in the alternative splicing variant, and the protein was putatively identified as a type of truncated membrane protein consisting of extramembrane, transmembrane and intramembrane regions lacking a signal peptide. Further, it was not a non- classical secretory protein. Five potential reference genes were screened for their potential as reliable standards to quantify the expression of TLR4 alternative spliced variants by real-time quantitative reverse-transcriptase polymerase chain reaction （qRT-PCR）. The stability of these reference genes was ranked using the geNorm and NormFinder programs, and ribosomal protein L4 （RPL4） and TATA box-binding protein （TBP） were found to be the two genes showing the most stable expression in the in vitro cultured peripheral blood mononuclear ceils （PBMCs） during heat shock. The mRNA level of the TLR4 gene （both classical and spliced） in stressed pigs increased significantly （P〈0.05）. Further, the expression levels of the alternative spliced variant of TLR4 （TLR4-ASV） showed a 2-3 folds increase in heat-stressed PBMCs as compared to control pigs. The results of the present study suggested that heat shock might modulate the host immune response by regulating the expressions of TLR4 and its alternative splicing variant.

关键词： altemative splicing variant Bama miniature pig toll-like receptor 4 heat stress

Establishment and characterization of immortalized bovine male germline stem cell line

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Journal of Integrative Agriculture 2017年第11期16卷 2547-2557页

作者： LEI Qi-jing PAN Qin MA ju-hong ZHOU Zhe LI Guang-peng CHEN Shu-lin HUA Jin-lian College of Veterinary Medicine Shaanxi Centre of Stem Cells Engineering and TechnologyNorthwest A&F University Key Laboratory for Mammalian Reproductive Biology and Biotechnology Ministry of Education/Inner Mongolia University

Male germline stem cells （mGSCs） are unique adult germ cells with self-renewal potential and spermatogenesis function in the testis. However, further studies are needed to establish a long-term cultural system of mGSCs in vitro, especially for large animals such as bovine mGSCs. In this study, we first established a stable immortalized bovine male germline stem cell line by transducing Simian virus 40 （SV40） large T antigen. The proliferation of these cells was improved significantly. These cells could express spermatogonial stem cell （SSC）-specific markers, such as PLZF, PGP9.5, VASA, LIN28A, and CD49F, both in the mRNA and protein levels. Additionally, these cells could be differentiated into three germ layer cells to enter meiosis, form colonies, and proliferate in the seminiferous tubules of busulfan-induced infertile mice. The immortalized bovine mGSCs maintain the criteria of mGSCs.

关键词： male germline stem cells immortalization bovine proliferation cell transplantation testis

Ultrafast shape change and joining of small-volume materials using nanoscale electrical discharge

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Nano Research 2015年第7期8卷 2143-2151页

作者： Cheng-Cai Wang Qing-Jie Li Liang Chen Yong-Hong Cheng jun Sun ZhioWei Shan ju Li Evan Ma Center for Advancing Materials Performance from the Nanoscale (CAMP-Nano) & Hysitron Applied Research Center in China (HARCC) State Key Laboratory for Mechanical Behavior of Materials Xi'an Jiaotong University Xi'an 710049 China State Key Laboratory of Electrical Insulation and Power Equipment Xi'an Jiaotong University Xi'an 710049 China Department of Nuclear Science and Engineering and Department of Materials Science and Engineering Massachusetts Institute of Technology 77 Massachusetts Avenue Cambridge Massachusetts 02139 USA Department of Materials Science and Engineering Johns Hopkins University Baltimore Maryland 21218 USA SJS Limited 101 Xihuan Road Jingzhou Hubei 434024 China

Using nanoscale electrical-discharge-induced rapid Joule heating, we developed a method for ultrafast shape change and joining of small-volume materials. Shape change is dominated by surface-tension-driven convection in the transient liquid melt, giving an extremely high strain rate of N106 s-1. In addition, the heat can be dissipated in small volumes within a few microseconds through thermal conduction, quenching the melt back to the solid state with cooling rates up to 108 K.s-1. We demonstrate that this approach can be utilized for the ultrafast welding of small-volume crystalline Mo （a refractory metal） and amorphous Cu49Zr51 without introducing obvious microstructural changes, distinguishing the process from bulk welding.

关键词： electrical discharge, welding, nanoscale, metallic glass, metal

Identification of Contaminations Hiding Beneath the α- and β-Subunits of Partially Purified Nitrogenase MoFe Protein on the Sodium Dodecyl Sulfate Gel

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Journal of Integrative Plant Biology 2005年第11期47卷 1364-1371页

作者： Hui-Na ZHOU Ying ZHAO Shao-Min BIAN Jian-Feng ZHAO Fei REN Huang-Ping WANG ju-Fu HUANG Key Laboratory of Photosynthesis and Environmental Molecular Physiology Institute of Botany the Chinese Academy of Sciences Beijing 100093 China Graduate School of the Chinese Academy of Sciences Beijing 100039 China China Agricultural University Beijing 100094 China Fujian Normal University Fuzhou 350007 China

To identify the unknown proteins that would contaminate the α- and β-subunits of nitrogenase MoFe protein on sodium dodecyl sulfate-polyacrylamide gel electrophoresis （SDS-PAGE）, the partially purified MoFe protein （Avl） preparation was obtained from Azotobacter vinelandii Lipmann OP by chroma- tography on DEAE-cellulose （DE52） and Sephacryl S-200 columns and analyzed by PAGE and matrix- assisted laser desorption/ionization time-of-flight （MALDI-TOF） mass spectrometry. The Av 1 preparation was shown to have two main bands at the position of the α- and β-subunits of crystalline Avl on the SDS gel. However, on the anoxic native PAGE, in addition to the Avl band, the preparation was shown to have three other main bands that migrated slower than Av 1. Of these three main bands, the protein with the fastest migration was identified as bacterioferritin elsewhere. The proteins on the other two bands, termed Upper and Middle, were suggested to be two different homopolymers with the same apparent subunit electrophoretic mobilities as the α- and β-subunits of Avl, respectively. By analysis of MALDI-TOF mass spectrometry, the Upper was identified as GroEL, which belongs to the heat shock protein 60 family, and the Middle was identified as glucose-6-phosphate isomerase （PGI）. In our preparation, anoxic native electrophoresis indicated that GroEL was composed of 14 identical subunits and that PGI was composed of 10 identical subunits. This is the first report of PGI, with so many subunits. The contaminating proteins in the Av 1 preparation, mainly GroEL and PGI, could be totally or partially removed from Av 1 if the shoulders and center of the elution peak were collected separately from the Sephacryl S-200 column and the center fraction was purified further by Q-Sepharose developed with an NaC1 concentration gradient. Thus, Avl with more than 90% purity was obtained. Obviously, this modified method is useful for the purification of mutant MoFe proteins with a high purity.

关键词： Azotobacter vinelandii chaperonin GroEL glucose-6-phosphate isomerase nitrogenase Av 1.

Effects of long-term green manure application on the content and structure of dissolved organic matter in red paddy soil

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Journal of Integrative Agriculture 2018年第8期17卷 1852-1860页

作者： GAO Song-juan GAO ju-sheng CAO Wei-dong ZOU Chun-qin HUANG Jing BAI Jinshun DOU Fu-gen College of Resources and Environmental Sciences Center for ResourcesEnvironment and Food Security/Key Laboratory of Plant-Soil InteractionsMinistry of EducationChina Agricultural UniversityBeijing100193P.R.China Key Laboratory of Plant Nutrition and Fertilizer Ministry of Agriculture/Institute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural SciencesBeijing 100081P.R.China Red Soil Experimental Station in Hengyang Chinese Academy of Agricultural SciencesQiyang 426182P.R.China Soil and Fertilizer Institute Qinghai Academy of Agriculture and Forestry SciencesQinghai UniversityXining 810016P.R.China Texas A&M Agri Life Research Center at Beaumont Texas77713USA

Dissolved organic matter(DOM) plays important roles in soil biogeochemistry activity and nutrients transportation in soils, but studies regarding the long-term effects of green manures on the content and structure of DOM in red paddy soil have not been reported yet. A long-term green manure experiment established in 1982 was utilized to test the DOM contents in different treatments, and the spectral characteristics of DOM were investigated by using ultraviolet-visible(UV-Vis) spectrometry and Fourier transform infrared(FTIR) spectrometry. The experiment included four cropping systems: ricerice-milk vetch(RRV), rice-rice-rape(RRP), rice-rice-ryegrass(RRG) and rice-rice-winter fallow(RRF), among them, milk vetch, rape, and ryegrass are popular winter green manure species in southern China. The results showed that the content of dissolved organic carbon(DOC), which is widely used to estimate the concentration of DOM, was significantly promoted after the incorporation of green manures compared with the other sampling stages. The contents of aromatic groups and the degree of humification of DOM increased in RRV and RRP, suggesting more complex compositions of the soil DOM after long-term application of milk vetch and rape. The contents of phenol, alcohol and carboxylic acid group at the mature stage of early rice were significantly higher than those at the stage of after green manures turned over, especially for the RRV treatment. The absorption ratio of FTIR indicated that winter plantation of rape increased the aromatic-C/aliphatic-C ratio, and ryegrass increased the aromatic-C/carboxyl-C ratio. In conclusion, long-term planting of milk vetch and rape as green manures increased the degree of aromaticity, humification and average molecular weight of DOM, and made the DOM more stable in red paddy soil.

关键词： green manure red paddy soil dissolved organic matter ultraviolet-visible spectra Fourier transform infrared spectra

The Relationship between Magnetic Gradient and Magnetic Shear in Five Super Active Regions Producing Great Flares

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Chinese Journal of Astronomy and Astrophysics 2006年第4期6卷 477-488页

作者： Hai-Min Wang Hui Song ju Jing Vasyl Yurchyshyn Yuan-Yong Deng Hong-Qi Zhang David Falconer Jing Li National Astronomical Observatories Chinese Academy of Sciences Beijing 100012 Big Bear Solar Observatory New Jersey Institute of Technology Big Bear City CA 92314 USA Marshall Space Flight Center SD50 Huntsville AL 35812 USA Institute for Astronomy University of Hawaii 2680 Woodlawn Drive Honolulu HI 96822 USA

We study the magnetic structure of five well-known active regions that produced great flares （X5 or larger）. The six flares under investigation are the X12 flare on 1991 june 9 in AR 6659, the X5.7 flare on 2000 july 14 in AR 9077, the X5.6 flare on 2001 April 6 in AR 9415, the X5.3 flare on 2001 August 25 in AR 9591, the X17 flare on 2003 October 28 and the X10 flare on 2003 October 29, both in AR 10486. The last five events had corresponding LASCO observations and were all associated with Halo CMEs. We analyzed vector magnetograms from Big Bear Solar Observatory, Huairou Solar Observing Station, Marshall Space Flight Center and Mees Solar Observatory. In particular, we studied the magnetic gradient derived from line-of-sight magnetograms and magnetic shear derived from vector magnetograms, and found an apparent correlation between these two parameters at a level of about 90%. We found that the magnetic gradient could be a better proxy than the shear for predicting where a major flare might occur： all six flares occurred in neutral lines with maximum gradient. The mean gradient of the flaring neutral lines ranges from 0.14 to 0.50 G km^-1, 2.3 to 8 times the average value for all the neutral lines in the active regions. If we use magnetic shear as the proxy, the flaring neutral line in at least one, possibly two, of the six events would be mis-identified.

关键词： Sun: activity -- Sun: flares -- Sun: magnetic fields -- Sun: coronal mass ejections (CMEs)

Synthesis and Crystal Structure of[(2 , 4-Me_2C_5H_5)_2NdC =CC_6H_5]_2

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Chemical Research in Chinese Universities 1994年第4期10卷 308-312页

作者： Zhang, Suo-bo Liu, ju-zheng Wei, Ge-cheng Jia, Heng-qing Chen, Wen-qi

he reaction of (2, 4-C_7H_11)_3Nd with HC=CC_6H_5 in toluene leads to [(2, 4-C_7H_11)_2NdC = CC_6H_5]_2 , which crystallizes in the space group P_cab with unit cell pa-rameters a=1 . 0489 (11), b=1.8033 (12), c=2. 1064 (15)nm and Z=4 , least-squares refinement based on 2323 observed reflections converged to a final R valueof 0. 08. The molecular structure shows that the complex is a dimer, in which two(2,4-C_7H_11)_2Nd units are connected by asymmetrical alkynide bridges with indepen-dent Nd-C(alkynide) bond lengths of 0. 2552(25)and 0. 2560(24)nm, Nd-C=C angles of 124. 1(17)° and 134. 2(17)°.

关键词： Synthesis Structure Tris (2 4-dimethylpentadienyl ) neodymium.Phenylacetylene

Effects of Cropping System Change for Paddy Field with Double Harvest Rice on the Crops Growth and Soil Nutrient

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Agricultural Sciences in China 2007年第9期6卷 1115-1123页

作者： ZENG Xi-bai SUN Nan GAO ju-sheng WANG Bo-ren LI Lian-fang Institute of Agricultural Environment and Sustainable Development Chinese Academy of Agricultural Sciences/Key Laboratory of Agro Environment and Climate Change Ministry of Agriculture Beijing 100081 P.R.China Institute of Agricultural Resources and Regional Planning Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China

The effects of the cropping system change for paddy field with double harvest rice on crops growth and soil nutrient in red soil were studied. The results indicated that the economic benefit and the ratio of the output to input were all increased in terms of the market price for the crops under various treatments. The greatest economic benefit was obtained in the treatment of paddy-upland rotation, and the corresponding economic benefit was increased by 34.7, 21.4, and 2.2% in comparison with that of control （rice-rice-astragali）, pasture, and upland cropping treatments. The economic benefits in pasture and upland cultivation treatments were increased by I 1.0 and 31.8%, respectively, when compared with that of the control treatment （CK）. The ratio of output to input in pasture, paddy-upland rotation, and upland cropping treatments was enhanced by 0.9, 0.6, and 0.3, respectively, in comparison with that of control. To grow pasture is beneficial for improving soil fertility since the contents of soil organic matter, total nitrogen, total phosphorus, and available phosphorus are all enhanced significantly. However, the concentrations of the soil available nitrogen, the total potassium, the available potassium were somewhat reduced in all the treatments, suggesting that increasing the input of nitrogen, particularly potassium, was necessary under the present fertilization level. Based on the conditions of fertility, climate, cultivation, and management of paddy field with double harvest rice in red soil regions, it is feasible to alter the cultivation system of paddy field with bad irrigation condition. In particular, cultivation systems such as pasture and paddy-upland rotation can be selected to extend because better economic benefit and improvement of soil fertility in the purpose region were obtained.

关键词： cropping system crop growth economic benefit soil nutrient

Chitosan hydrogel encapsulated with LL-37 peptide promotes deep tissue injury healing in a mouse model

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Military Medical Research 2020年第4期7卷 409-417页

作者： Xu Yang Jing-Lin Guo Jing Han Rui-juan Si Pan-Pan Liu Zi-Rui Zhang Ai-Min Wang ju Zhang School of Nursing Qingdao UniversityQingdao 266021China

Background:LL-37 peptide is a member of the human cathelicidin family,and has been shown to promote the healing of pressure ***,the low stability of this peptide within the wound environment limits its clinical ***(CS)hydrogel is commonly used as a base material for wound dressing ***:CS hydrogel(2.5%w/v)was encapsulated with *** of the product was examined in cultured NIH3 T3 *** on immune response was examined by measuring tumor necrosis factor-α(TNF-α)release from RAW 264.7 macrophages upon exposure to *** activity was assessed using Staphylococcus *** effect on pressure ulcers was examined using a mouse ***,adult male C57 BL/6 mice were subjected to skin pressure using magnets under a 12/12 h schedule for 21 *** were randomized to receive naked LL-37(20μg),chitosan gel containing 20μg LL-37(LL-37/CS hydrogel)or hydrogel alone under the ulcer bed(n=6).A group of mice receiving no intervention was also included as a ***:LL-37/CS hydrogel did not affect NIH3 T3 cell *** a concentration of 1–5μg/ml,LL-37/CS inhibited TNF-αrelease from *** 5μg/ml,LL-37/CS inhibited the growth of Staphylococcus *** area of the pressure ulcers was significantly lower in mice receiving LL-37/CS hydrogel in comparison to all other 3 groups on days 11(84.24%±0.25%),13(56.22%±3.91%)and 15(48.12%±0.28%).Histological examination on days 15 and 21 showed increased epithelial thickness and density of newly-formed capillary with naked LL-37 and more so with LL-37/*** expression of key macromolecules in the process of angiogenesis(i.e.,hypoxia inducible factor-1α(HIF-1α)and vascular endothelial growth factor-A(VEGF-A))in wound tissue was increased at both the mRNA and protein ***:Chitosan hydrogel encapsulated with LL-37 is biocompatible and could promote the healing of pressure ulcers.

关键词： LL-37 Chitosan hydrogel Deep tissue injury Pressure ulcer Cathelicidin