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Chemoselective labeling-based spermatozoa glycan imaging reveals abnormal glycosylation in oligoasthenotspermia

Chinese Chemical Letters 2024年第4期35卷 475-480页

作者： Lijia Xu Tong Zhong Wei zhao Bing Yao Lin Ding Huangxian ju State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210023China Department of Reproductive Medical Center Jinling HospitalSchool of MedicineNanjing UniversityNanjing 210002China Chemistry and Biomedicine Innovation Center(ChemBIC) Nanjing UniversityNanjing 210023China Center of Clinical Laboratory Science The Affiliated Cancer Hospital of Nanjing Medical University&Jiangsu Cancer Hospital&Jiangsu Institute of Cancer ResearchNanjing 210009China

Spermatogenesis, maturation, capacitation and fertilization are precisely regulated by glycosylation. However, the relationship between altered glycosylation patterns and the onset and development of reproductive disorders is unclear, mainly limited by the lack of in situ imaging techniques for spermatozoa glycosylation. We developed an efficient and highly specific spermatozoa glycan imaging technique based on the robust chemoselective labeling of sialic acid(Sia) and N-acetyl-D-galactosamine(Gal/GalNAc). We further proposed a “tandem glycan chemoselective labeling” strategy to achieve simultaneous imaging of two types of glycans on spermatozoa. We applied the developed method to the spermatozoa from oligozoospermic patients and diabetic mice and found that these spermatozoa showed higher levels of Sia and Gal/Gal NAc expression than the normal groups. Moreover, spermatozoa from diabetic mice showed a severe decrease in number, viability, and forward motility, suggesting that in vivo glucose metabolism disorders may lead to an elevated level of spermatozoa glycosylation and have a correlation with the development of oligoasthenotspermia. Our work provides a research tool to reveal the relationship between glycosylation modification and spermatozoa quality, and a promising clue for the development of glycan-based reproductive markers.

关键词： Spermatozoa Glycan Chemoselective labeling Oligoasthenotspermia Fluorescence imaging

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Studies on the Pathogenicity Differentiation of Verticillium dahliae on Cotton Varieties

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Agricultural Sciences in China 2001年第1期 57-63页

作者： ZHOU zhao-hua ZHANG Tian-zhen PAN Jia-ju CHEN zhao-xia JIANG Wei-min Department of Agronomy Nanjing Agricultural University Nanjing 210095 Department of Agronomy Nanjing Agricultural University Nanjing 210095 Department of Agronomy Nanjing Agricultural University Nanjing 210095 Department of Agronomy Nanjing Agricultural University Nanjing 210095 Nanjing Agricultural Training School

Verticillium dahliae may be classified into the two basic groups, defoliate and non-defoliate, in terms of the pathogenicity reaction of 19 isolates of Verticillium dahliae on 16 cotton varieties. According to the comprehensive resistant or susceptible expression of 8 defoliate isolates on 8 upland cotton varieties R02,R04, R05, R06, R08, R09, R11 and R14, the defoliate isolates were for the first time divided into three categories: strong pathogenic (XS4 as the representative), mediun with inclination to strong pathogenic (T9 as the representative) and medium pathogenic (VD8 as the representative). Through the resistance identification of varieties such as R05, R06, R08, R09, R11 and R14, the defoliate isolate VD8 originated from Nantong of Jiangsu Province may be efficiently distinguished from the defoliate isolate 19 originated from USA. In nondefoliate category, utilizing 4 upland varieties R15, R14, R13 and R10, it might be possible to identify the different pathogenic genes in 7 isolates XJ4, XJ1, AY, VD404, VD326, LY, BP2 and may be nominated as No. 1 to No. 7 biological races respectively. The upland variety R01 is capable of resisting to all the isolates used in the experiments. While upland variety R12, may be used to identify strongly pathogenic isolate, which displayed a specific function in identifying strong pathogenic isolate strains.

关键词： Cotton Verticillium wilt Verticillium dahliae Pathogenicity

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产时手术矫治危重先天性心脏病1例

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中国医药 2024年第5期19卷 755-757页

作者：李刚赵举王晟贺彦李燕娜车辑袁慧王强首都医科大学附属北京安贞医院小儿心脏中心北京100029 首都医科大学附属北京安贞医院重症医学中心北京100029 首都医科大学附属北京安贞医院麻醉中心北京100029 首都医科大学附属北京安贞医院妇产科北京100029 首都医科大学附属北京安贞医院输血科北京100029 首都医科大学附属北京安贞医院检验科北京100029

危重先天性心脏病(先心病)患儿生后即面临生命危险,一旦病情恶化,预后极差。针对这种术后危重状态可能导致的严重不良后果,在1例入住首都医科大学附属北京安贞医院的胎儿心脏结构异常孕妇的诊疗中我们首次选择新生儿娩出后即启动手术的... 详细信息

危重先天性心脏病(先心病)患儿生后即面临生命危险,一旦病情恶化,预后极差。针对这种术后危重状态可能导致的严重不良后果,在1例入住首都医科大学附属北京安贞医院的胎儿心脏结构异常孕妇的诊疗中我们首次选择新生儿娩出后即启动手术的多学科救治模式,来规避术前的高风险。母孕期胎儿诊断为完全型肺静脉异位引流,于本院经剖宫产娩出胎儿后,立即转入心脏手术室,明确诊断,完成必要术前检查后开始麻醉,完成手术矫治,手术过程、术后恢复顺利。该救治模式通过新生儿娩出后即刻的早期手术有望改善危重先心病患儿的预后,降低死亡率,提高远期健康水平。

关键词：先天性心脏病完全型肺静脉异位引流新生儿

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One-pot synthesis and characterization of a new, branched polycarbosilane bearing allyl groups

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Chinese Chemical Letters 2007年第6期18卷 754-757页

作者： Tian Hua Huang zhao ju Yu Xu Min He Mu He Huang Li Fu Chen Hai Ping Xia Li Tong Zhang Advanced Materials Laboratory Department of Materials Science & Engineering College of Chemistry & Chemical Engineering Xiamen University Xiamen 361005 China

A highly branched polycarbosilane bearing allyl groups has been prepared by a one-pot synthesis with chloromethyltrichlorosilane, chloromethylmethyldichlorosilane and allyl chloride as the starting materials. The resultant polymer, with the approximate formula [SiH1.2(CH3)0.71(CH2CHCH2)0.09CH2]n, has been characterized by 1H, 13C, and 29Si NMR, GPC, TG and elemental analysis. It could be cross-linked thermally at 170 °C in the absence of oxygen. Pyrolysis of the polymer gave a ceramic with a yield of about 70%.

关键词： Polycarbosilane One-pot synthesis Silicon carbide precursor Ceramic precursor

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Single-center report of 5-year follow-up on 94 patients underwent transmyocardial laser revascularization

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Chinese Medical Journal 2007年第22期120卷 1982-1985页

作者： QU Zheng ZHENG ju-bing ZHANG zhao-guang Department of Cardiac Surgery Beijing Anzhen Hospital Capital Medical University Beijing 100029 China

Background Transmyocardial laser revascularization （TMLR） has been used in the treatment of patients with end-stage coronary artery disease （CAD） since 1990. The aim of this study was to evaluate the long-term effectiveness of TMLR in patients with diffuse CAD.Methods Ninety-four consecutive patients underwent TMLR in one center from july 1997 to December 2000. The follow-up data of these patients were obtained through face-to-face, mail questionnaires, or telephone interviews in july 2004 and December 2004. Four cases failed to respond. Mean follow-up time was （5.5±1.0） years. Results Mean Canadian Cardiovascular Society （CCS） angina scores of TMLR patients were 3.1±0.8 at baseline, 1.7±0.9 at 1 year （P〈0.05）, 1.7±0.9 at 3 years （P〈0.05）, and 1.9±0.9 at 5 years （P〈0.05）. At an average of 5 years, 69% of the patients had 〉 1 angina class reduction, mean NYHA class level （1.9±0.9） ameliorated compared to the baseline （2.5±0.7, P〈0.001）, the rate of re-hospitalization was 2.7 times/person. Kaplan-Meier survival rate was 87% at 1 year, 69% at 3 years and 64% at 5 years. The causes of death were attributed more to heart failure （58.9%） and myocardial infraction （14.7%） after TMLR. The patients with no angina relief, or who died after TMLR, had a higher percentage of preoperative unstable anginas or prior myocardial infraction compared to the survivors. The assorted shapes of myocardial laser channels were detected in some patients by the color Doppler velocity technique. Conclusions TMLR provided a long-term improvement in the quality of life, including CCS angina class or NYHA heart functional class for about 70% of Chinese patients with severely disabling angina pectoris. The various myocardial laser channels would always be visible after TMLR. 5-years after TMLR as a sole therapy, the survival rate of the patients was 64%.

关键词： transmyocardial laser revascularization angina coronary artery disease

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Analysis and improving ways for water condition in winter wheat field of dryland in subhumid areas of the Loess Plateau

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Journal of Environmental Sciences 1998年第3期10卷 85-94页

作者： Zhong zhaozhan, zhao jubao  Institute of Agrometeorology, Chinese Academy of Agricultural Sciences, Beijing 100081,China 

Based on the soil moisture data from the locating experiments from 1986—1990, and using the water balance method, the water supply and demand state in the field of winter wheat, and the ways for improving water condition in dryland of dry subhumid area of the Loess Plateau were studied. The results suggested that a low precipitation satisfying ratio of 42.9%—58.8% appears in the growing period of winter wheat, and the yield, to a great extent depended on the water that was stored in deep soil layer in the previous rainy season. The filed trial results showed that tillage in the summer fallow period,straw cover, soil moisture regulation with adequate fertilization, crop rotation and proper cropping system could be the efficient ways for improving the water condition,and for the exploitation and utilization of natural water resources(both precipitation and soil water) in the winter wheat field of dryland. 

关键词： soil moisture water balance precipitation meet ratio dry subhumid area dryland rainfed agriculture.

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Flexible incidence angle scanning surface plasmon resonance microscopy for morphology detection with enhanced contrast

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Photonics Research 2024年第6期12卷 1213-1221页

作者： LINGKE WANG JINGYU MI SHUQI WANG WENRUI LI ju TANG JIAWEI ZHANG JIWEI ZHANG JIANLIN zhao Key Laboratory of Light Field Manipulation and Information Acquisition Ministry of Industry and Information Technologyand Shaanxi Key Laboratory of Optical Information TechnologySchool of Physical Science and TechnologyNorthwestern Polytechnical UniversityXi’an 710129China

Surface plasmon resonance microscopy(SPRM)has been massively applied for near-field optical measurement,sensing,and imaging because of its high detection sensitivity,nondestructive,noninvasive,wide-field,and label-free imaging ***,the transverse propagation characteristic of the surface plasmon wave generated during surface plasmon resonance(SPR)leads to notable“tail”patterns in the SPR image,which severely deteriorates the image ***,we propose an incidence angle scanning method in SPRM to obtain a resonance angle image with exceptional contrast that significantly mitigates the adverse effects of“tail”*** resonance angle image provides the complete morphology of the analyzed samples and enables two-dimensional quantification,which is incapable in conventional *** effectiveness of the method was experimentally verified using photoresist square samples with different sizes and two-dimensional materials with various geometric *** edges of samples were fully reconstructed and a maximum fivefold increase in the image contrast has been *** method offers a convenient way to enhance the SPRM imaging capabilities with low cost and stable performance,which greatly expands the applications of SPRM in label-free detection,imaging,and quantification.

关键词： angle resonance surface

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Status and circulation characteristics of soil water in dryland field of southeast Shanxi Province

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Journal of Environmental Sciences 1998年第4期10卷 27-34页

作者： Zhong zhao zhan, zhao ju bao, Mei Xu rong Institute of Agrometeorology, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Based on the observed soil water data from experimental site located in southeast of Shanxi Province, the physical characteristics of soil water, crop effect on soil moisture, and the field water circulation pattern were studied by using the water balance method. The results suggested that soil water deficit often exists in fields of these areas. From May to june, the amount of water deficit in bare land rises to the maximum (232 8 mm) and falls to the minimum (66 6 mm) from August to September. By comparison, because of crop transpiration, both soil water deficit and dry soil layer in cultivated land are 15 1—40 4 mm more and 20—70 mm deeper respectively than those of bare land. Crops mainly planted in these areas have a relatively weak utilization ability to soil water. Winter wheat has the highest utilization ability to soil water among the crops planted in these areas. The soil water utilization ability of winter wheat is 26 2%—30 6% and winter wheat can use soil water that lies in soil layer below a depth of over 200 cm. Spring corn and millet can only consume soil water with the maximum ability of 13 4% and the deepest layer of 0—50 cm or 0—100cm, which shows that the soil water utilization ability of winter wheat is higher than that of spring crops. After crop is ripe, more than 41% of available soil water remains unused in field. So, increasing soil water storage and improving crop utilization ability to soil water by adopting efficient agrotechnique measures are the main ways for improving agricultural productivity in dry farming areas of Northern China.

关键词： soil water field water deficit crop utilization ability to soil water dry land southeast of Shanxi Province.

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An overview of autophagy in the differentiation of dental stem cells

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BIOCELL 2024年第1期48卷 47-64页

作者： XITONG zhao TIANjuAN ju XINWEI LI CHANGFENG LIU LULU WANG LI-AN WU State Key Laboratory of Oral&Maxillofacial Reconstruction and Regeneration National Clinical Research Center for Oral DiseasesShaanxi Clinical Research Center for Oral DiseasesDepartment of Pediatric DentistrySchool of StomatologyThe Fourth Military Medical UniversityXi’an710032China

Dental stem cells(DSCs)have attracted significant interest as autologous stem cells since they are easily accessible and give a minimal immune *** properties and their ability to both maintain self-renewal and undergo multi-lineage differentiation establish them as key players in regenerative *** many regulatory factors determine the differentiation trajectory of DSCs,prior research has predominantly been based on genetic,epigenetic,and molecular *** evidence suggests that DSC differentiation can also be influenced by autophagy,a highly conserved cellular process responsible for maintaining cellular and tissue homeostasis under various stress *** comprehensive review endeavors to elucidate the intricate regulatory mechanism and relationship between autophagy and DSC *** achieve this goal,we dissect the intricacies of autophagy and its ***,we elucidate its pivotal roles in impacting DSC differentiation,including osteo/odontogenic,neurogenic,and angiogenic ***,we reveal the regulatory factors that govern autophagy in DSC lineage commitment,including scaffold materials,pharmaceutical cues,and the extrinsic *** implications of this review are far-reaching,underpinning the potential to wield autophagy as a regulatory tool to expedite DSC-directed differentiation and thereby promote the application of DSCs within the realm of regenerative medicine.

关键词： Dental stem cells Cell differentiation Autophagy Mitophagy Autophagy regulation

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Simulation and Experimental Method for Microwave Oven

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Journal of Electronic Science and Technology of China 2009年第2期7卷 188-191页

作者： Han-Ji ju Qing zhao School of Physical Electronics University of Electronic Science and Technology of China Chengdu 610054 China.

The simulation software, HFSS （high frequency structure simulator）, is introduced in microwave oven design. In the cold test, a network analyzer is used to measure the reflection coefficient （S11） of the cavity under empty and loaded states over the frequency range from 2.448 GHz to 2.468 GHz. In the hot test, a piece of wet thermal paper and an infrared thermal imaging camera are used to measure the electric field distributions on the mica and turntable. In the cold test, the simulation agrees well with the experiment no matter in empty state or loaded state. In the hot test, the simulation agrees well with the experiment in general in empty state and approximately in loaded state. The little difference in both cold and hot test may be due to that the model in simulation is not absolutely identical with that in experiment or the inadequate precision of infrared thermal imaging camera.

关键词： Index Terms-Far-infrared thermal imaging camera,microwave oven simulation network analyzer wetthermal paper.

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