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Red Onion (Allium Cepa L.) Methanolic Extract Increases Extracellular Nucleotide Hydrolysis in Rat Serum

Open Journal of Applied Sciences 2020年第12期10卷 864-876页

作者： Janayne Luihan Silva Bruno Quintanilha Faria Vinícius Marques Arruda Fernanda Cardoso da Silva joyce ferreira da costa guerra Cristina Ribas Fürstenau Instituto de Ciências Biológicas Universidade Federal de Minas Gerais Belo Horizonte Brazil Curso de Biotecnologia Universidade Federal de Uberlandia Patos de Minas Brazil Programa de Pós-graduaç o em Biotecnologia Instituto de Biotecnologia Universidade Federal de Uberlandia Patos de Minas Brazil Centro de Ciências Naturais e Humanas Universidade Federal do ABC Santo André Brazil

Ectonucleotidases are enzymes involved in nucleotides metabolism. The amount of circulating nucleotides may modulate distinct pathophysiological processes in the vasculature, including platelet aggregation and vascular tone. Onion (Allium cepa L.) phenolic compounds modulate enzymatic activity. The present study evaluated the total phenolic content of red onion methanolic extract, its antioxidant capacity, and its ability to interfere in nucleotides hydrolyses in rat serum. Total phenolic content was determined with the Folin-Ciocalteau reagent using gallic acid as a standard, while total flavonoid content was obtained through the aluminum chloride colorimetric method with quercetin as a standard. Antioxidant capacity was evaluated from the ability of the extract to scavenge ABTS·+ and DPPH· radicals. ATP, ADP, AMP, and p-Nph-5'-TMP hydrolyses were colorimetrically determined in response to different onion extract concentrations (0, 125, 250, 500, or 1000 μg/mL). Phenolic content of the extract was 54.35 mg GAE 100 g-1 sample, while flavonoid content was 7.22 mg quercetin g-1 sample. The IC50 value for ABTS·+ was 374.13 ± 7.52 μg/mL, while it was 440.29 ± 15.17 μg/mL for DPPH·. Red onion extract increased ADP and p-Nph-5'-TMP hydrolysis. The results confirmed that red onion contains high content of antioxidant, mainly flavonoids, and high antioxidant capacity. Additionally, biochemical studies suggest that the increased ADP breakdown may be important to regulate vascular processes. As it occurs for other enzymes, the antioxidant capacity of onion extract may neutralize reactive oxygen species (ROS) formation and favor ectonucleotidase activity and the hydrolysis of ADP, a major platelet agonist.

关键词： Red Onion Flavonoids Reactive Oxygen Species Ectonucleotidases Vascular Homeostasis

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Food and Nutritional Security in the Refugee Women’s Life Narratives

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Food and Nutrition Sciences 2023年第11期14卷 1031-1042页

作者： Juliana Vidal Vieira guerra Valdecyr Herdy Alves Brenda Caroline Martins da Silva Tatiana do Socorro dos Santos Calandrini Giovanna Rosario Soanno Marchiori Ediane de Andrade ferreira Maria Bertilla Lutterbach Riker Branco Diego Pereira Rodrigues Departamento de Nutriç &#227o Aplicada Instituto de Nutri&#231&#227o Universidade do Estado do Rio de Janeiro Rio de Janeiro Brasil Programa Acadêmico em Ciências do Cuidado em Saúde Escola de Enfermagem Aurora de Afonso Costa Universidade Federal Fluminense Niterói Brasil Instituto de Ciências da Saúde Universidade Federal do Pará Belém Brasil Centro de Ciências da Saúde Universidade Federal de Roraima Boa Vista Brasil

Objective: To understand, based on the life narratives of refugee women, food and nutritional security. Method: Qualitative, descriptive, exploratory study, Narratives of life method, by daniel Bertaux. Conducted with 11 participants, refugee women residing in the state of Rio de Janeiro, Brazil. Results: The meanings and feelings evidenced in the life narratives of refugee women expressed the insecurities and weaknesses with food in the resettlement. Final Considerations: Given the meanings and feelings regarding food in the refuge, it is expected that the present study will contribute to the elaboration of new action plans and intersectoral public policies of agile action to meet the demands of the refugee population, in the attempt to mitigate starvation, poverty, social differences and the compromised health status of the refugee population.

关键词： Food Security Feeding Behavior Refugees Human Migration Human Right to Adequate Food

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Stimulation of the P6 Acupuncture Point for Prophylaxis of Nausea and Vomiting in Pregnant Women Submitted to Cesarean Section: A Blinded Clinical Trial

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Open Journal of Anesthesiology 2013年第3期3卷 161-164页

作者： Luiza Helena Castelo Branco Luciana Cavalcante Lima Maria Célia ferreira costa Marilia Santos Lira Raquel Queiroz guerra de Andrade Coelho Tania Cursino de Menezes Couceiro Anne danielle Soares Eusa Maria Belarmino Alcoforado Maria Emilia Carvalho Instituto de Medicina Integral Prof. Fernando Figueira (IMIP) Recife Brazil.

In pregnant women subjected to spinal anesthesia for a Cesarean section, episodes of nausea and vomiting are common both during and following surgery. Acupuncture for the prophylaxis and treatment of these complications has been gaining in popularity due to its low cost, simplicity, absence of side effects and confirmed efficacy. This study investigated the efficacy of stimulating the P6 acupoint in conjunction with the use of dexamethasone as prophylaxis for nausea and vomiting in pregnant women submitted to spinal anesthesia for a Cesarean section. The patients (n = 100) were randomly distributed into two groups. In the first group (n = 50), a site located one centimeter laterally from P6 was stimulated. This is not a true acupuncture point (sham acupuncture). In the second group (n = 50), P6 was stimulated. In both groups, 4 mg of dexamethasone were administered intravenously. A questionnaire was used to obtain information on the occurrence of nausea and vomiting during surgery and in the first 12 hours postpartum. The chi-square test and Fisher’s exact test were used to assess differences between the groups. Age and physical status were similar in both groups. The incidence of nausea during surgery was 32% (n = 16) in the control group and 22% (n = 11) in the P6 group (p > 0.05). In the first 12 hours following surgery, nausea occurred in 16% of the women in the control group (n = 6) and in 4% in the P6 group (n = 4) (p = 0.045). The incidence of vomiting in the control group was 12% (n = 6) during surgery and 10% (n = 5) in the postoperative period compared to 8% (n = 4) and 4% (n = 2), respectively, in the P6 group (p > 0.05). Although these differences were not statistically significant with the exception of the incidence of nausea in the first 12 hours postpartum, a reduction occurred in the incidence of all the outcomes evaluated in the P6 group.

关键词： Obstetric Surgery Cesarean Section Complications Nausea Vomiting Prophylaxis Acupuncture

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