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The Effects of Treatment on Serum Hepcidin and Iron Homeostasis in HIV-1-Infected In-dividuals

World Journal of AIDS 2015年第3期5卷 151-160页

作者： Joel da Cunha Luciana Morganti ferreira Maselli jovino dos santos ferreira Celso Spada Sergio Paulo Bydlowski Laboratory of Genetics and Molecular Hematology(LIM31) University of Sao Paulo School of Medicine(FMUSP)Sao PauloBrazil Clinical Analysis Department Health Sciences CenterFederal University of Santa Catarina(UFSC)FlorianopolisBrazil Research Division Pro-Sangue Hemocentro de Sao Paulo FoundationSao PauloBrazil Hemotherapy Service of University Hospital Federal University of Santa Catarina(UFSC)FlorianopolisBrazil

Background: Hepcidin is the principal regulator of iron absorption and its tissue distribution. Its correlation with iron homeostasis in individuals infected with human immunodeficiency virus type-1 (HIV-1) treated with different regimens of highly active antiretroviral therapy (HAART) was investigated. Methods: Serum hepcidin levels were determined in 448 volunteers. Of these, 372 were HIV-1-infected individuals, and 93 did not receive HAART (ART-naïve) while 279 received HAART consisting of a non-nucleoside reverse transcriptase inhibitor (NNRTI-based) and protease inhibitors (PI-based);both were used in association with a nucleoside reverse transcriptase inhibitor (NRTI). Seventy-six additional HIV-1 seronegative individuals were enrolled in the study. The following parameters were quantified: hematological parameters, iron biomarkers and markers of infection (CD4+ and CD8+ T-cells), and HIV-1 RNA (viral load). Results: Serum hepcidin, iron and ferritin levels, as well as the marker of infection, CD4+ T-cells, were significantly lower in the ART-naïve group compared with other groups. Additionally, transferrin saturation, iron binding capacity, hemoglobin level and erythrocyte level were not significantly different, and anemia was not observed in the different groups. Conclusions: HIV-1 infection affected serum hepcidin, iron and ferritin levels in the ART-naïve group, and the different HAART regimens restored the levels of hepcidin and iron homeostasis in HIV-1-infected individuals who have undetectable HIV-1 RNA levels.

关键词： Hepcidin Iron Homeostasis HIV-1 HAART ART-Naive CD4+ T-Cells

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Temporal and spatial differences in human activities performed in Urban Green Spaces of Vilnius(Lithuania)

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Geography and Sustainability 2024年第2期5卷 302-317页

作者： Luís Valença Pinto Carla Sofia santos ferreira Paulo Pereira Research Centre for Natural Resources Environment and Society(CERNAS) Polytechnic Institute of CoimbraBencanta3045-601 CoimbraPortugal Environmental Management Laboratory Mykolas Romeris UniversityVilniusLithuania Polytechnic Institute of Coimbra Applied Research InstituteRua da MisericórdiaLagar dos Cortiços-S.Martinho do Bispo3045-093 CoimbraPortugal

Urban green spaces(UGS)are relevant to city well-being,as recognized by the United Nations’Sustainable Development Goals(SDGs).However,few studies have studied the temporal use of *** work assessed the seasonal,weekly,and daily use of three urban green spaces(Vingis Park,Bernardino Garden,and Jomantas Park)in Vilnius(Lithuania).The study is based on an on-site observation-based survey,which recorded users’characteristics,activities,and weather conditions during summer and *** results showed that UGS’s seasonal,weekly,and daily use differed according to park and users’*** with a higher diversity of facilities had a high seasonal difference in the number of observed *** numbers were higher in the summer for activities with children,social activities,sports,and water activities than in the *** Park had the lowest variability in user *** variables were linked to changes in users’*** precipitation and lower temperature were associated with reducing the number of users and the diversity of registered *** of the stationary activities were observed during *** diversity of the observed activities was associated with the available facilities rather than the park *** distribution of stationary activities was spatially correlated with facility/equipment(benches,playgrounds,sports,and fitness equipment)and proximity to water *** results of this study are relevant for UGS design,planning,and management.

关键词： Urban parks Seasonal differences Temporal use Time of day Spatial distribution of UGS users

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Changes in calcium accumulation and utilization efficiency and their impact on recycling,immobilization,and export across the oil palm cycle

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Oil Crop Science 2024年第3期9卷 143-150页

作者： Ismael de Jesus Matos Viégas Luma Castro de Souza Eric Victor de Oliveira ferreira Milton Garcia Costa Glauco André dos santos Nogueira Vitor Resende do Nascimento Candido ferreira de Oliveira Neto Capanema Campus Federal Rural University of the Amazon(UFRA)Av.Barao de CapanemaCaixa D'agua5514-6434ParaBrazil Center of Agricultural and Environmental Sciences Federal University of Maranhao(UFMA)ChapadinhaBR-222km 04SNBoa Vista65500000MaranhaoBrazilc Capitao Poço Campus Federal Rural University of the Amazon(UFRA)Tv.Pau AmareloS/n-Vila Nova68650-000Capitao PoçoParaBrazil Department of Soil Science Sao Paulo State University(Unesp)School of Agricultural and Veterinarian SciencesJaboticabalVia de Acesso Prof.Paulo Donato CastelaneCastellane-S/N-Vila Industrial14884-900Sao PauloBrazil Institute of Agricultural Sciences(ICA) Federal Rural University of the Amazon(UFRA)Estr.Principal da Ufra2150Curio-Utinga66077-580BelemParaBrazil

Effective calcium(Ca)management is crucial for optimizing oil palm cultivation and enhancing crop *** study aimed to gain insights into the dynamics of Ca concentration,accumulation,exportation,immobilization,and recycling in various oil palm organs relative to plant *** experiment was conducted at the Agropalma enterprise site in the northeastern region of Para State,Brazil,evaluating seven plant age treatments:2,3,4,5,6,7,and 8 years *** a completely randomized design with four *** results demonstrated an age-related increase in Ca concentration in petioles,rachis,arrows,male inflorescences,peduncles,and ***,Ca accumulation exhibited an upward trend in all organs with progressing plant ***,the study revealed an enhanced Ca use efficiency across all plant organs in correlation with the age of oil palm *** findings underscore the dynamic nutritional demands of oil palm,influencing Ca immobilization,cycling,and export throughout its developmental stages.

关键词： Elaeis guineensis Jacq Ca cycling Ca export Ca immobilization Ca use efficiency Plant nutrition Amazon

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A facile strategy for tuning the density of surface-grafted biomolecules for melt extrusion-based additive manufacturing applications

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Bio-Design and Manufacturing 2024年第3期7卷 277-291页

作者： I.A.O.Beeren G.dos santos P.J.Dijkstra C.Mota J.Bauer H.ferreira Rui L.Reis N.Neves S.Camarero-Espinosa M.B.Baker L.Moroni Department of Complex Tissue Regeneration MERLN Institute for Technology-Inspired Regenerative MedicineMaastricht University6229 ER MaastrichtThe Netherlands 3B’s Research Group I3Bs–Research Institute on BiomaterialsBiodegradables and BiomimeticsHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineUniversity of MinhoAveParkZona Industrial da Gandra4805-017 BarcoGuimarãesPortugal ICVS/3B’s-PT Government Associate Laboratory 4806-909 Braga/GuimarãesPortugal POLYMAT University of the Basque Country UPV/EHU20018 Donostia/San SebastiánSpain IKERBASQUE Basque Foundation for Science48009 BilbaoSpain

Melt extrusion-based additive manufacturing(ME-AM)is a promising technique to fabricate porous scaffolds for tissue engi-neering ***,most synthetic semicrystalline polymers do not possess the intrinsic biological activity required to control cell *** of biomolecules on polymeric surfaces of AM scaffolds enhances the bioactivity of a construct;however,there are limited strategies available to control the surface ***,we report a strategy to tune the surface density of bioactive groups by blending a low molecular weight poly(ε-caprolactone)5k(PCL5k)containing orthogonally reactive azide groups with an unfunctionalized high molecular weight PCL75k at different *** porous three-dimensional(3D)scaf-folds were then fabricated using a high weight percentage(75 wt.%)of the low molecular weight PCL *** a proof-of-concept test,we prepared films of three different mass ratios of low and high molecular weight polymers with a thermopress and reacted with an alkynated fluorescent model compound on the surface,yielding a density of 201-561 pmol/cm^(2).Subsequently,a bone morphogenetic protein 2(BMP-2)-derived peptide was grafted onto the films comprising different blend compositions,and the effect of peptide surface density on the osteogenic differentiation of human mesenchymal stromal cells(hMSCs)was *** two weeks of culturing in a basic medium,cells expressed higher levels of BMP receptor II(BMPRII)on films with the conjugated *** addition,we found that alkaline phosphatase activity was only significantly enhanced on films contain-ing the highest peptide density(i.e.,561 pmol/cm^(2)),indicating the importance of the surface *** together,these results emphasize that the density of surface peptides on cell differentiation must be considered at the cell-material ***,we have presented a viable strategy for ME-AM community that desires to tune the bulk and surface functionality via blending of(m

关键词： Additive manufacturing Blending Surface functionalization Surface density Click chemistry Human

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Extrahepatic cholestasis associated with paracoccidioidomycosis:Challenges in the differential diagnosis of biliopancreatic neoplasia

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World Journal of Gastrointestinal Oncology 2024年第6期16卷 2531-2540页

作者： Jose Sebastiao dos santos Vitor de Moura Arrais William Jose Rosseto ferreira Ricardo Ribeiro Correa Filho Mariangela Ottoboni Brunaldi Rafael Kemp Ajith Kumar Sankanrakutty Jorge Elias Junior Fernando Bellissimo-Rodrigues Roberto Martinez Edson Zangiacomi Martinez Jose Celso Ardengh Faculdade de Medicina de Ribeirao Preto da Universidade de Sao Paulo(FMRP-USP) Departamento de Cirurgia e AnatomiaRibeirao Preto 14049-900Sao PauloBrazil Universidade de Sao Paulo Faculdade de Medicina de Ribeirao Preto da Universidade de Sao Paulo(FMRP-USP)Departamento de PatologiaRibeirao Preto 14048900Sao PauloBrazil Universidade de Sao Paulo Faculdade de Medicina de Ribeirao Preto da Universidade de Sao Paulo(FMRP-USP)Departamento de Imagens MédicasHematologia e Oncologia ClínicaRibeirao Preto 14048900Sao PauloBrazil Universidade de Sao Paulo Faculdade de Medicina de Ribeirao Preto da Universidade de Sao Paulo(FMRP-USP)Departamento de Medicina SocialRibeirao Preto 14015-010Sao PauloBrazil Universidade de Sao Paulo Faculdade de Medicina de Ribeirao Preto da Universidade de Sao Paulo(FMRP-USP)Departamento de Clínica MédicaRibeirao Preto 14015-010Sao PauloBrazil Universidade de Sao Paulo Faculdade de Medicina de Ribeirao Preto da Universidade de Sao Paulo(FMRP-USP)Departamento de Cirurgia e AnatomiaRibeirao Preto 14049-900Sao PauloBrazil and Hospital MoriahServico de Endoscopia DigestivaSao Paulo 04084-002Sao PauloBrazil

BACKGROUND Paracoccidioidomycosis(PCM)may involve the hepatic pedicle and peripan creatic lymph nodes,cause damage to the bile duct and manifest,exceptionally,in combination with extrahepatic cholestasis(EHC),making investigation and treatment *** To investigate the management of patients with visceral PCM admitted with *** All patients diagnosed with PCM treated in a public,tertiary teaching hospital between 1982 and 2020 were retrospectively *** also identified with EHC were allocated to two groups according to the treatment approach for the purpose of comparing clinical,laboratory,and imaging findings,resources used for etiological diagnosis,treatment results,and *** analyses were performed using the linear mixed-effects model(random and fixed effects),which was adjusted using the PROC MIXED procedure of the SAS®9.0 software,and Fisher’s exact *** Of 1645 patients diagnosed with PCM,40(2.4%)had *** these,20(50.0%)lived in the rural area and 29(72.5%)were men,with a mean age of 27.1 years(3-65 years).Jaundice as first symptom and weight loss of at least 10 kg were observed in 16 patients(40.0%),and a mass in the head of the pancreas was observed in 8(20.0%).The etiological diagnosis was made by tissue collection during surgery in 4 cases(10.0%)and by endoscopic methods in 3 cases(7.5%).Twenty-seven patients(67.5%)received drug treatment alone(Group 1),whereas 13(32.5%)underwent endoscopic and/or surgical procedures in combination with drug treatment(Group 2).EHC was significantly reduced in both groups(40.7% in Group 1,with a mean time of 3 months;and 38.4% in Group 2,with a mean time of 7.5 months),with no statistically significant difference between *** recurrence rates,associated mainly with treatment nonadherence,were similar in both groups:37% in Group 1 and 15.4% in Group *** mortality rate was 18.5% in Group 1 and 23% in Group 2,with survival estimates of 71.3% and 72.5%,respectively,with

关键词： Cholestasis Jaundice Obstructive Blastomycosis Paracoccidioides Diagnosis Treatment

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Personalized medicine:Clinical oncology on molecular view of treatment

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World Journal of Clinical Oncology 2024年第8期15卷 992-1001页

作者： Rafick Costa dos santos Da Silva Nathalia de Andrade Simon AndréAlves dos santos Gabriel De Melo Olegário Jayne ferreira Da Silva Naide Oliveira Sousa Manuel Alvarez Troncoso Corbacho Fabrício Freire de Melo Instituto Multidisciplinar em Saúde Universidade Federal da BahiaVitória Da Conquista 45029-094BahiaBrazil

Cancer,the second leading global cause of death,impacts both physically and *** treatments such as surgeries,chemotherapy,and radiotherapy have adverse effects,driving the need for more precise *** medicine enables more targeted *** mapping,alongside other molecular biology approaches,identifies specific genes,contributing to accurate *** review addresses,in clinical use,a molecular perspective on *** like alpha-fetoprotein,beta-human chorionic gonadotropin,5-hydroxyindoleacetic acid,programmed death-1,and cytotoxic T lymphocyte-associated protein 4 are explored,providing valuable ***,with an emphasis on artificial intelligence,revolutionizes the analysis of biological data,offering more accurate *** like liquid biopsy are emphasized for early *** medicine guides therapeutic strategies based on the molecular characteristics of the tumor,as evidenced in the molecular subtypes of breast *** allow personalized treatments,highlighting the role of trastuzumab and endocrine *** the benefits,challenges persist,including high costs,tumor heterogeneity,and ethical *** obstacles requires collaboration,ensuring that advances in molecular biology translate into accessible benefits for all.

关键词： Oncology Neoplasia Molecular biology Personalized medicine Molecular Markers Cancer

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Antimicrobial and synergistic effects of lemongrass and geranium essential oils against Streptococcus mutans,Staphylococcus aureus,and Candida spp.

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World Journal of Critical Care Medicine 2024年第3期13卷 63-74页

作者： Patrícia Michelle Nagai de Lima Thaís Cristine Pereira Lara Steffany de Carvalho Letícia ferreira dos santos Carlos Eduardo Rocha Oliveira Lucas de Paula Ramos Maria Cristina Marcucci Amjad Abu Hasna Luciane Dias de Oliveira Department of Biosciences and Oral Diagnosis Institute of Science and TechnologySão Paulo State UniversitySão Josédos Campos 12245000SPBrazil Anhembi Morumbi University School of MedicineSão Josédos Campos 12230-002SPBrazil Department of Restorative Dentistry Endodontics DivisionInstitute of Science and TechnologySão Paulo State UniversitySão Josédos Campos 12245000São PauloBrazil

BACKGROUND The oral cavity harbors more than 700 species of bacteria,which play crucial roles in the development of various oral diseases including caries,endodontic infection,periodontal infection,and diverse oral *** To investigate the antimicrobial action of Cymbopogon Schoenanthus and Pelargonium graveolens essential oils against Streptococcus mutans,Staphylococcus aureus,Candida albicans,***,and *** Minimum microbicidal concentration was determined following Clinical and Laboratory Standards Institute *** synergistic antimicrobial activity was evaluated using the Broth microdilution checkerboard method,and the antibiofilm activity was evaluated with the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide *** were analyzed by one-way analysis of variance followed by the Tukey post-hoc test(P≤0.05).RESULTS *** and *** essential oils were as effective as 0.12%chlorhexidine against *** and *** monotypic biofilms after 24 *** 24 h *** essential oil at 0.25%was more effective than the nystatin group,and *** essential oil at 0.25%was as effective as the nystatin *** *** and *** essential oils are effective against ***,***,***,***,and *** at different concentrations after 5 min and 24 h.

关键词： Antifungal effect Lemongrass Geranium Candida albicans Candida dubliniensis Candida krusei Staphylococcus aureus Streptococcus mutans

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Immunohistochemistry panel segregates molecular types of hepatocellular carcinoma in Brazilian autopsy cases

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World Journal of Gastroenterology 2016年第27期22卷 6246-6256页

作者： Aloísio Felipe-Silva Alda Wakamatsu Cinthya dos santos Cirqueira Venancio Avancini ferreira Alves LIM 14-Patologia Hepatica Faculdade de Medicina da USP01246-903 Sao Paulo SPBrazil Department of Pathology Medical Research Laboratory LIM14University of Sao Paulo School Of Medicine01246-903 Sao Paulo SPBrazil

AIM: To assess the distribution of proteins coded by genes reported as relevant for the molecular classification of hepatocellular carcinoma (HCC).METHODS: In this retrospective cross-sectional study, the following clinicopathological data were analyzed in 80 autopsied HCC patients: sex, age, ethnicity, alcohol intake, infection with hepatitis B and/or C virus, infection with human immunodeficiency virus, prior treatment, basic and immediate causes of death, liver weight, presence of cirrhosis, number and size of nodules, gross pattern, histological grade and variants, architectural pattern, invasion of large veins, and presence and location of extrahepatic metastases. The protein products of genes known to be involved in molecular pathogenesis of HCC, including epidermal growth factor receptor (EGFR), MET, keratin 19 (K19), vimentin, beta-catenin, mechanistic target of rapamycin (mTOR), extracellular signaling-related kinase (ERK)1, ERK2, Ki67, cyclin D1, caspase 3 and p53, were detected by immunohistochemistry on tissue microarrays. The expression levels were scored and statistically assessed for correlation with HCC ***: Infection with hepatitis C virus was identified in 49% of the 80 autopsy patients, cirrhosis in 90%, advanced tumors in 95%, and extrahepatic metastases in 38%. Expression of K19, p53 and ERK1 correlated to high-grade lesions. Expression of ERK1, nuclear beta-catenin, cyclin D1 and ERK2 correlated to higher rates of cell proliferation as determined by Ki67. Expression of MET, EGFR (> 0) and caspase 3 correlated with lower histological grades. Expression of EGFR correlated to that of caspase 3, and overexpression of EGFR (≥ 200/300) was observed in low-grade tumors more frequently (grades 1 and 2: 67% vs grade 3: 27% and grade 4: 30%). Expression of ERK1 was associated with that of K19 and vimentin, whereas expression of ERK2 was associated with that of cyclin D1, MET and membrane beta-catenin. Expression of vimentin

关键词： Hepatocellular carcinoma Epidermal growth factor receptor Autopsy Immunohistochemistry Liver Classification

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High-pressure metamorphic rocks in the Borborema Province, Northeast Brazil: Reworking of Archean oceanic crust during proterozoic orogenies

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Geoscience Frontiers 2020年第6期11卷 2221-2242页

作者： Alanielson da Câmara Dantas ferreira Elton Luiz Dantas Ticiano JoséSaraiva dos santos Reinhardt A.Fuck Mahyra Tedeschi Instituto de Geociências Universidade de Brasília(UnB)70910-900Brasília-DFBrazil Instituto de Geociências Universidade Estadual de Campinas(UNICAMP)Departamento de Geologia e Recursos Naturais13083-970Campinas-SPBrazil Instituto de Geoci^encias Universidade Federal de Minas Gerais(UFMG)31270-901Belo HorizonteMGBrazil

We present the first evidence of Archean oceanic crust submitted to Proterozoic high-pressure(HP)metamorphism in the South American ***-Nd and Lu-Hf isotopic data combined with U-Pb geochronological data from the Campo Grande area,Rio Grande do Norte domain,in the Northern Borborema Province,reflect a complex Archean(2.9 Ga and 2.6 Ga)and Paleoproterozoic(2.0 Ga)evolution,culminating in the Neoproterozoic Brasiliano/Pan-African orogeny(ca.600 Ma).The preserved mafic rocks contain massive poikiloblastic garnet and granoblastic amphibole with variable proportions of plagioclase+diopside in symplectitic texture,typical of high-pressure *** clinopyroxene-garnet amphibolites and the more common garnet amphibolites from the Campo Grande area are exposed as rare lenses within an Archean migmatite *** amphibolite lenses represent 2.65 Ga juvenile tholeiitic magmatism derived from depleted mantle sources(positive values of+3.81 to+30.66)later enriched by mantle metasomatism(negative εNd(t)values of-7.97).Chondrite and Primitive Mantle-normalized REE of analyzed samples and discriminant diagrams define two different oceanic affinities,with E-MORB and OIB *** Eu anomalies(Eu/Eu*=0.75-0.95)indicate depletion of plagioclase in the *** zircon cores of 3.0-2.9 Ga in analyzed samples indicate that the Neoarchean tholeiitic magmatism was emplaced into 2923±14 Ma old Mesoarchean crust(εNd(t)--2.58 and Nd TDM=3.2 Ga)of the Rio Grande do Norte *** age of retro-eclogite facies metamorphism is not yet completely *** suggest that two high-grade metamorphic events are recognized in the mafic rocks:the first at 2.0 Ga,recorded in some samples,and the second,at ca.600 Ma,stronger and more pervasive and recorded in several of the mafic rock *** Neoproterozoic zircon grains are found in symplectite texture as inclusions in the garnet grains and represent the age of HP conditions in the *** zircon grains show a

关键词： Archean oceanic crust Proterozoic orogens Borborema province

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Spatiotemporal Variability of Methane over the Amazon from Satellite Observations

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Advances in Atmospheric Sciences 2016年第7期33卷 852-864页

作者： Igor Oliveira RIBEIRO] Rodrigo Augusto ferreira de SOUZA Rita Valeria ANDREOLI Mary Toshie KAYANO Patrfcia dos santos COSTA Post-Graduate Program in Climate and Environment CLIAMBINPA/UEAAv.Andre Araujo2936Campus 11Aleixo69060-001ManausAMBrasil Amazonas State University Superior School of TechnologyAv.Darcy Vargas1200Parque 10 de Novembro69065-020ManausAMBrasil National Institute for Space Research Center For Weather Forecasting and Climate ResearchAv.dos Astronautas175812227-010Sao Josd dos CamposSPBrasil

The spatiotemporal variability of the greenhouse gas methane （CH4） in the atmosphere over the Amazon is studied using data from the space-borne measurements of the Atmospheric Infrared Sounder on board NASA＇s AQUA satellite for the period 2003-12. The results show a pronounced variability of this gas over the Amazon Basin lowlands region, where wetland areas occur. CH4 has a well-defined seasonal behavior, with a progressive increase of its concentration during the dry season, followed by a decrease during the wet season. Concerning this variability, the present study indicates the important role of ENSO in modulating the variability of CH4 emissions over the northern Amazon, where this association seems to be mostly linked to changes in flooded areas in response to ENSO-related precipitation changes. In this region, a CH4 decrease （increase） is due to the E1 Nifio-related （La Nifia-related） dryness （wetness）. On the other hand, an increase （decrease） in the biomass burning over the southeastern Amazon during very dry （wet） years explains the increase （decrease） in CH4 emissions in this region. The present analysis identifies the two main areas of the Amazon, its northern and southeastern sectors, with remarkable interannual variations of CH4. This result might be useful for future monitoring of the variations in the concentration of CH4, the second-most important greenhouse gas, in this area.

关键词： climate variability methane satellite data Amazon region

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