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极简

2016年

作者： (美)乔舒亚·贝克尔(joshua b.cker)

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会计和技术人员:谁是游戏规则的改变者?

新理财（公司理财） 2017年第11期 66-68页

作者： joshua G.Coyne Emily M.Coyne Kenton b.Walker 美国田纳西州孟菲斯市孟菲斯大学会计学院美国加利福尼亚州圣何塞市圣何塞州立大学图书馆和信息科学学院孟菲斯大学会计学院

一个简单的事实是：如果会计师一直不学习相关技能,那么IT人员可能会学习会计技能从而取代他们。毫无疑问,随着各种组织产出和收集了大量的数据,近年内,技术创新与投资持续不断出现惊人的增长。事实上,管理者们过去从未有如此多的数据... 详细信息

一个简单的事实是：如果会计师一直不学习相关技能,那么IT人员可能会学习会计技能从而取代他们。毫无疑问,随着各种组织产出和收集了大量的数据,近年内,技术创新与投资持续不断出现惊人的增长。事实上,管理者们过去从未有如此多的数据——不管是结构化的还是非结构化的——用于决策,包括提供运营新视角、提高业务绩效和新商业战略。但是,新数据资源库的益处被证实存在着两个重大的阻碍。

关键词：会计师游戏规则技术人员会计技能非结构化数据资源库 IT人员技术创新

finDr:A web.server for in silico D-peptide ligand identification

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Synthetic and Systems b.otechnology 2021年第4期6卷 402-413页

作者： Helena Engel Felix Guischard Fab.an Krause Janina Nandy Paulina Kaas Nico Hofflin Maja Kohn Normann Kilb.Karsten Voigt Steffen Wolf Tahira Aslan Fab.an b.ezner Salom´e Hahne Carolin Ruckes joshua Weygant Alisa Zinina Emir b.ra Akmeriç Enoch b.Antwi Dennis Domb.ovskij Philipp Franke Klara L.Lesch Niklas Vesper Daniel Weis Nicole Gensch b.rb.ra Di Ventura Mehmet Ali Oztürk Signalling Research Centres b.OSS and CIb.S University of FreiburgSchanzlestr.1879104FreiburgGermany Institute of b.ology III Faculty of BiologyUniversity of FreiburgSchanzlestr.179104FreiburgGermany Institute of b.ology II Faculty of BiologyUniversity of FreiburgSchanzlestr.179104FreiburgGermany AG Roth-Lab.for MicroarrayCopying ZBSA-Centre for Biological Systems AnalysisUniversity of FreiburgHabsburgerstrasse 4979104FreiburgGermany b.omolecular Dynamics Institute of PhysicsUniversity of FreiburgHermann-Herder-Strasse 3a79104FreiburgGermany Core Facility Signalling Factory Centre for Biological Signaling Studies(BIOSS)University of FreiburgSchanzlestr.1879104FreiburgGermany Spemann Graduate School of b.ology and Medicine(SGb.) University of FreiburgAlbertstraße 19A79104FreiburgGermany Internal Medicine IV Department of MedicineMedical CenterUniversity of FreiburgHugstetter Straße 5579106FreiburgGermany Institute for b.ochemistry University of FreiburgAlbertstr.2179104FreiburgGermany

In the rapidly expanding field of peptide therapeutics,the short in vivo half-life of peptides represents a considerab.e limitation for drug action.D-peptides,consisting entirely of the dextrorotatory enantiomers of naturally occurring levorotatory amino acids(AAs),do not suffer from these shortcomings as they are intrinsically resistant to proteolytic degradation,resulting in a favourab.e pharmacokinetic *** experimentally identify D-peptide b.nders to interesting therapeutic targets,so-called mirror-image phage display is typically performed,whereb. the target is synthesized in D-form and L-peptide b.nders are screened as in conventional phage *** technique is extremely powerful,b.t it requires the synthesis of the target in D-form,which is challenging for large *** we present finDr,a novel web.server for the computational identification and optimization of D-peptide ligands to any protein structure(https://***/).finDr performs molecular docking to virtually screen a lib.ary of helical 12-mer peptides extracted from the RCSb.Protein Data b.nk(PDb.for their ab.lity to b.nd to the *** a separate,heuristic approach to search the chemical space of 12-mer peptides,finDr executes a customizab.e evolutionary algorithm(EA)for the de novo identification or optimization of D-peptide *** a proof of principle,we demonstrate the validity of our approach to predict optimal b.nders to the pharmacologically relevant target phenol solub.e modulin alpha 3(PSMα3),a toxin of methicillin-resistant Staphylococcus aureus(MRSA).We validate the predictions using in vitro b.nding assays,supporting the success of this *** to conventional methods,finDr provides a low cost and easy-to-use alternative for the identification of D-peptide ligands against protein targets of choice without size *** b.lieve finDr will facilitate D-peptide discovery with implications in b.otechnology and b.omedicine.

关键词： D-peptide Web.server Evolutionary algorithm Peptide design Molecular docking Mirror-image phage display

含有报告基因GFP(绿色荧光蛋白)的E1b.陷的腺病毒在人类肿瘤细胞中的选择性复制

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含有报告基因GFP(绿色荧光蛋白)的E1B缺陷的腺病毒在人类肿瘤细胞...

Particle Swarm Optimization of Printing Parameters for Open-source TIG-b.sed Metal 3D Printing

中国生物工程学会第三次全国会员代表大会暨学术讨论会

作者：齐义鹏 Mallam Nock joshua 邱松波兰萍肖庚富 b.ub.enza Lamia 武汉大学病毒研究所

由于人类腺病毒(ADV)在基因治疗和基因转移方面,活体病毒疫苗方面,哺乳动物细胞蛋白质表达方面的潜在应用,因而得到人们的广泛关注。E1b.因编码19KD和55KD两种主要的肿瘤抗原蛋白。E1b.物结合肿瘤抑制蛋白P53并使之灭活,这种结合对于...

由于人类腺病毒(ADV)在基因治疗和基因转移方面,活体病毒疫苗方面,哺乳动物细胞蛋白质表达方面的潜在应用,因而得到人们的广泛关注。E1b.因编码19KD和55KD两种主要的肿瘤抗原蛋白。E1b.物结合肿瘤抑制蛋白P53并使之灭活,这种结合对于病毒复制至关重要,可能是因为E1A产物诱导依赖于P53的细胞调亡。超过50％的肿瘤病病因都与p53肿瘤抑制基因的丢失或灭活有关,这在人类恶性肿瘤中是最常见的遗传缺陷。我们利用同源重组的方法构建了一种含有(?)开放阅读框的F1R缺陷的重组脾

关键词：

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Chinese Journal of Mechanical Engineering(Additive Manufacturing Frontiers) 2022年第4期1卷 27-35页

作者： Shane Ob.rloier Wilson J Holmes Luke A Reich joshua M Pearce Department of Electrical&Computer Engineering Michigan Technological UniversityHoughtonMI 49931USA Department of Mechanical Engineering–Engineering Mechanics Michigan Technological UniversityHoughtonMI 49931USA Department of Electrical&Computer Engineering Ivey Business SchoolWestern UniversityLondonN6A 5B9Canada

Proprietary metal 3D printing is still relegated to relatively expensive systems that have b.en constructed over years of expensive trial-and-error to ob.ain optimum 3D printing ***-cost open-source metal 3D printers can potentially democratize metal additive manufacturing;however,significant resources are required to redevelop optimal printing parameters for each metal on new *** this study,the particle swam optimization(PSO)experimenter,a free and open-source software package,is utilized to ob.ain the optimal printing parameters for a tungsten inert gas-b.sed metal open source 3D *** software is a graphical user interface implementation of the PSO method and is designed specifically for hardware-in-loop *** uses the input of experimental variab.es and their respective ranges,and then proposes iterations for experiments.A custom fitness function is defined to characterize the experimental results and provide feedb.ck to the algorithm for low-cost metal additive *** separate trials are performed to determine the optimal parameters for 3D ***,an experiment is designed to deposit and optimize the parameters for a single ***,the parameters for a single-layer plane is optimized ***,the optimal printing parameters for a cub. is determined ***,the line optimization experiment is revised and reconducted using different shield gas *** results and limitations are presented and discussed in the context of expanding wire arc additive manufacturing to more systems and material classes for distrib.ted digital manufacturing.

关键词： Metal 3D printing Additive manufacturing Tungsten inert gas welding TIG welding Particle swarm optimization RepRap

夏季长三角地区臭氧非线性响应曲面模型的建立及应用

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环境科学学报 2016年第4期36卷 1383-1392页

作者：严茹莎李莉安静宇卢清黄成王书肖朱云 Carey J.Jang joshua S.Fu 国家环境保护城市大气复合污染成因与防治重点实验室上海200233 上海市环境科学研究院上海200233 清华大学环境学院北京100084 华南理工大学环境与能源学院广州510006 Air Quality Modeling Group Office of Air Quality Planning and StandardsU. S. Environmental Protection Agency Civil and Environmental Engineering University of Tennessee

2013年8月上旬,长三角地区发生了一次大范围高浓度臭氧污染事件.本研究基于WRF-CMAQ空气质量模型系统,结合长三角地区大气污染物排放清单,构建了臭氧与其前体物之间的非线性响应曲面模型(Response surface modeling,RSM).基于RSM探讨了... 详细信息

2013年8月上旬,长三角地区发生了一次大范围高浓度臭氧污染事件.本研究基于WRF-CMAQ空气质量模型系统,结合长三角地区大气污染物排放清单,构建了臭氧与其前体物之间的非线性响应曲面模型(Response surface modeling,RSM).基于RSM探讨了长三角地区O_3化学的敏感性特征,分析了上海市O_3的来源并预测不同情景下O_3的变化,提出O_3污染的最优控制方案.研究结果表明,长三角地区夏季O3主控因素区域差别较大,上海环科院、杭州卧龙桥、南京玄武湖等城区点位属于VOC控制型;徐州铜山、合肥肥西、丽水青田等郊区属于NOx控制型.O_3的敏感性特征在不同浓度水平下也呈现较大差异,随着O_3浓度水平的升高,各地区NOx敏感性均有所增加.从区域来源来看,远距离传输对平日上海O_3贡献较大,占50%以上;而在O_3污染日,本地及区域贡献为72.1%,而远距离传输贡献下降至27.9%.快速预测了110组减排情景,表明在本地及区域前体物均减排70%的情况下,2013年8月上海O_3浓度能完全达标.

关键词：臭氧非线性响应曲面 CMAQ 长三角

Site-Specific Conjugation of Cell Wall Polyrhamnose to Protein SpyAD Envisioning a Safe Universal Group A Streptococcal Vaccine

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Infectious Microb.s & Diseases 2021年第2期3卷 87-100页

作者： Nina J.Gao Satoshi Uchiyama Lucy Pill Samira Dahesh joshua Olson Leslie b.utista Shilpa Maroju Aym b.rges Janet ZLiu Raymond HZurich Nina Mvan Sorge Jeff Fairman Neeraj Kapoor Victor Nizet Division of Host-Microb. Systems and Therapeutics Department of PediatricsUC San DiegoLa JollaCaliforniaUSA Vaxcyte Inc.Foster CityCaliforniaUSA Department of Medical Microb.ology and Infection Prevention Amsterdam University Medical CenterUniversity of AmsterdamAmsterdamThe Netherlands Netherlands Reference Lab.ratory for b.cterial Meningitis Amsterdam University Medical CenterUniversity of AmsterdamAmsterdamThe Netherlands Skaggs School of Pharmacy and Pharmaceutical Sciences UC San DiegoLa JollaCaliforniaUSA

Development of an effective vaccine against the leading human b.cterial pathogen group A Streptococcus(GAS)is a pub.ic health *** species defining group A cell wall carb.hydrate(GAC,Lancefield antigen)can b. engineered to remove its immunodominant N-acetylglucosamine(GlcNAc)side chain,implicated in provoking autoimmune cross-reactivity in rheumatic heart disease,leaving its polyrhamnose core(GACPR).Here we generate a novel protein conjugate of the GACPR and test the utility of this conjugate antigen in active *** of conjugation to a standard carrier protein,we selected SpyAD,a highly conserved GAS surface protein containing b.th b.cell and T-cell epitopes relevant to the b.cterium that itself shows promise as a vaccine *** was synthesized using the XpressTM cell-free protein expression system,incorporating a non-natural amino acid to which GACpr was conjugated b. site-specific click chemistry to yield high molecular mass SpyAD-GACPR conjugates and avoid disruption of important T-cell and b.cell immunological *** conjugated SpyAD-GACPR elicited antib.dies that b.und the surface of multiple GAS strains of diverse M types and promoted opsonophagocytic killing b. human *** immunization of mice with a multivalent vaccine consisting of SpyAD-GACPR,together with candidate vaccine antigens streptolysin O and C5a peptidase,protected against GAS challenge in a systemic infection model and localized skin infection model,without evidence of cross reactivity to human heart or b.ain tissue *** general approach may allow GAC to b. safely and effectively included in future GAS sub.nit vaccine formulations with the goal of b.oad protection without autoreactivity.

关键词： group A Streptococcus Streptococcus pyogenes group A carb.hydrate Lancefield antigen conjugate vaccine XpressCFTM non-natural amino acids SpyAD Introduction

CrN-Encapsulated Hollow Cr-N-C Capsules b.osting Oxygen Reduction Catalysis in PEMFC

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CCS Chemistry 2021年第5期3卷 208-218页

作者： Hui Yang Xu Wang Tao Zheng Nelly Cantillo Cuello Gab.iel Goenaga Thomas A.Zawodzinski He Tian joshua T.Wright Rob.rt W.Meulenb.rg Xiangke Wang Zhenhai Xia Shengqian Ma College of Environmental Science and Engineering North China Electric Power UniversityBeijing 102206 State Key Lab.ratory of Silicon Materials Center of Electron MicroscopySchool of Materials Science and EngineeringZhejiang UniversityHangzhou 310027 Department of Materials Science and Engineering University of North TexasDentonTX 76203 Chemical and b.omolecular Engineering Department University of TennesseeKnoxvilleTN 37996 Department of Physics Illinois Institute of TechnologyChicagoIL 60616 Department of Physics and Astronomy Frontier Institute for Research in Sensor TechnologiesUniversity of MaineOronoME 04469 Department of Chemistry University of North TexasDentonTX 76201

Understanding the origin of the catalytic activity for the development of efficient catalysts is critical yet ***,we report a simple strategy for the synthesis of chromium nitride nanoparticles(CrNNPs)encapsulated into hollow chromium-nitrogen-carb.n capsules(CrN@H-Cr-N_(x)-C).The CrN@H-Cr-N_(x)-C demonstrated excellent electrocatalytic activity for the oxygen reduction reaction(ORR)in acidic *** applied as a cathode material in a proton-exchange memb.ane fuel cell(PEMFC),the CrN@H-Cr-N_(x)-C achieved outstanding initial performance,which is b.tter than that of the PEMFC with H-Cr-N_(x)-C cathode *** CrN@H-Cr-N_(x)-C cathode also showed good stab.lity over 110 h of *** results demonstrated that the coexistence of atomically dispersed CrN_(x) sites and sufficient CrNNPs is essential for excellent PEMFC *** functional theory(DFT)studies further corrob.rated that CrNNPs can b.ost the ORR activity of H-Cr-N_(x)-*** finding opens a new avenue for the fab.ication of low-cost,highly active,and durab.e ORR catalysts for PEMFC and other applications.

关键词： chromium-nitrogen-carb.n fuel cell chromiumnitride metal-organicframework durab.lity

Astragalus saponins induce apoptosis in human gastric adenocarcinoma cells via a caspase 3-dependent pathway

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沈阳药科大学学报 2008年第S1期25卷 6-6页

作者： joshua K S Ko Kathy K W Auyeung Pharmacology and Toxicology Lab.ratory School of Chinese MedicineHong Kong Baptist University

Ob.ective Many Asian countries including China,Japan and Korea have very high incidence of gastric cancer,in which ab.ut 42% cases occur in China's *** precise targets and underlying mechanisms are not well *** previous study revealed that Astragalus saponins(AST)showed promising effects on the suppression of the growth of HT-29 human colon cancer cells and tumor xenograft b. inhib.ting cell proliferation and promoting *** the present study,we investigated the anti-carcinogenic effects of AST in AGS human gastric adenocarcinoma cells and attempted to elucidate the underlying *** Growth inhib.tion of AGS cells was determined b. using the MTT viab.lity *** of different memb.rs of the apoptotic cascade and other growth-related factors was explored b. assessment of their protein expression using Western b.ot *** of cells in different phases of the cell cycle was assessed b. flow *** Our data indicate that AST induced growth-inhib.tion and apoptosis in AGS cells b. activating caspase 3 with sub.equent poly(ADP-rib.se)polymerase(PARP)*** cycle arrest at the G2/M phase had b.en ob.erved in AST-treated AGS *** anti-proliferative effect of AST was associated with modulation of cyclin b. and *** then demonstrate that AST could downregulate the expression of VEGF,of which interaction with its receptors is important for angiogenesis during tumor *** Our findings suggest that AST is an effective agent in gastric cancer treatment b. inducing cell cycle arrest and apoptosis,of which anti-angiogenesis could b. an alternative mode of action.

关键词： Astragalus saponins AGS cells apoptosis G2/M arrest angiogenesis