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Effect of mucin 4 allele on susceptibility to experimental infection with enterotoxigenic F4 Escherichia coli in pigs fed experimental diets

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Journal of Animal Science and Biotechnology 2019年第4期10卷 981-989页

作者： Samantha O.Sterndale Danica J.Evans josephine p.mansfield Julie Clarke Shafi Sahibzada Sam Abraham Mark O’Dea David W.Miller Jae Cheol Kim John R.pluske Agriculture Sciences College of ScienceHealthEngineering and Education Murdoch UniversityMurdochWA 6150Australia CSIRO Health and Biosecurity PO BOX 10041AdelaideBC 5000Australia Antimicrobial Resistance and Infectious Disease Laboratory Murdoch UniversityMurdochWA 6150Australia AB Vista Asia pte.Ltd The MezzoWhampoa 329682Singapore

Background: This study investigated the validity of the DNA-marker based test to determine susceptibility to ETECF4 diarrhoea by comparing the results of two DNA sequencing techniques in weaner pigs following experimental infection with F4 enterotoxigenic Escherichia coli(ETEC-F4). The effects of diet and genetic susceptibility were assessed by measuring the incidence of piglet post-weaning diarrhoea(pWD), faecal E. coli shedding and the diarrhoea ***: A DNA marker-based test targeting the mucin 4 gene(MUC4) that encodes F4 fimbria receptor identified pigs as either fully susceptible(SS), partially or mildly susceptible(SR), and resistant(RR) to developing ETEC-F4 diarrhoea. To further analyse this, DNA sequencing was undertaken, and a significantly higher proportion of C nucleotides was observed for RR and SR at the Xba I cleavage site genotypes when compared to SS. However, no significant difference was found between SR and RR genotypes. Therefore, results obtained from Sanger sequencing retrospectively allocated pigs into a resistant genotype(MUC4–), in the case of a C nucleotide, and a susceptible genotype(MUC4+), in the case of a G nucleotide, at the single nucleotide polymorphism site. A total of 72 weaner pigs(age ~ 21 days), weighing 6.1 ± 1.2 kg(mean ± SEM), were fed 3 different diets:(i) positive control(pC) group supplemented with 3 g/kg zinc oxide(Zn O),(ii) negative control(NC) group(no Zn O or HAMSA),and(iii) a diet containing a 50 g/kg high-amylose maize starch product(HAMSA) esterified with acetate. At days five and six after weaning, all pigs were orally infected with ETEC(serotype O149:F4;toxins LT1, ST1, ST2 and EAST). The percentage of pigs that developed diarrhoea following infection was higher(p = 0.05) in MUC4+ pigs compared to MUC4– pigs(50% vs. 26.8%, respectively). Furthermore, pigs fed Zn O had less ETEC-F4 diarrhoea(p = 0.009) than pigs fed other diets, however faecal shedding of ETEC was similar(p > 0.05) between ***

关键词： Diarrhoea Escherichia coli F4 MUC4 Weaner pigs Zinc oxide

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