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High Sensitivity of Shotgun Metagenomic Sequencing in Colon Tissue Biopsy by Host DNA Depletion

Genomics, Proteomics & Bioinformatics 2023年第6期21卷 1195-1205页

作者： Wing yin Cheng Wei-Xin Liu yanqiang Ding Guoping Wang yu Shi Eagle S.H.Chu Sunny Wong joseph j.y.sung jun yu State Key Laboratory of Digestive Disease Institute of Digestive Disease and The Department of Medicine and TherapeuticsLi Ka Shing Institute of Health SciencesShenzhen Research InstituteThe Chinese University of Hong KongHong Kong Special Administrative Region 999077China Lee Kong Chian School of Medicine Nanyang Technology UniversitySingapore 639798Singapore

The high host genetic background of tissue biopsies hinders the application of shotgun metagenomic sequencing in characterizing the tissue *** proposed an optimized method that removed host DNA from colon biopsies and examined the effect on metagenomic *** or mouse colon biopsies were divided into two groups,with one group undergoing host DNA depletion and the other serving as the *** DNA was removed through differential lysis of mammalian and bacterial cells before *** impact of host DNA depletion on microbiota was compared based on phylogenetic diversity analyses and regression *** host DNA enhanced bacterial sequencing depth and improved species discovery,increasing bacterial reads by 2.46±0.20 folds while reducing host reads by 6.80%±1.06%.Moreover,2.40 times more of bacterial species were detected after host DNA *** was confirmed from mouse colon tissues,increasing bacterial reads by 5.46±0.42 folds while decreasing host reads by 10.2%±0.83%.Similarly,significantly more bacterial species were detected in the mouse colon tissue upon host DNA depletion(Py of shotgun metagenomic sequencing in bacteria detection in the biopsy,which may yield a more accurate taxonomic profile of the tissue microbiota and identify bacteria that are important for disease initiation or progression.

关键词： Gut microbiota Colon tissue Host DNAdepletion Shotgun metagenomic sequencing

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Intratumoral Bacteria Dysbiosis Is Associated with Human Papillary Thyroid Cancer and Correlated with Oncogenic Signaling Pathways

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Engineering 2023年第9期28卷 179-192页

作者： Shuang yu yanqiang Ding Xuejie Wang Siu Kin Ng Siting Cao Weixin Liu Zhuming Guo yubin Xie Shubin Hong Lixia Xu Xiaoxing Li jie Li Weiming Lv Sui Peng yanbing Li joseph j.y.sung jun yu Haipeng Xiao Department of Endocrinology The First Affiliated Hospital of Sun Yat-Sen UniversityGuangzhou 510080China Institute of Digestive Disease and Department of Medicine and Therapeutics State Key Laboratory of Digestive DiseaseThe Chinese University of Hong KongHong Kong 999077China Department of Head and Neck Surgery Sun Yat-Sen University Cancer CenterGuangzhou 510060China Clinical Trials Unit The First Affiliated Hospital of Sun Yat-Sen UniversityGuangzhou 510080China Institute of Precision Medicine The First Affiliated Hospital of Sun Yat-Sen UniversityGuangzhou 510080China Department of Oncology The First Affiliated Hospital of Sun Yat-Sen UniversityGuangzhou 510080China Department of Breast and Thyroid Surgery The First Affiliated Hospital of Sun Yat-Sen UniversityGuangzhou 510080China Lee Kong Chian School of Medicine Nanyang Technology UniversitySingapore 308232Singapore

Emerging evidence suggests that microbial dysbiosis plays vital roles in many human ***,knowledge of whether the microbial community in thyroid tumor is related to tumorigenesis remains *** this study,we aimed to explore the microbial community in thyroid tissues and its contribution to papillary thyroid cancer(PTC).In parallel,we performed microbial profiling and transcriptome sequencing in the tumor and adjacent normal tissues of a large cohort of 340 PTC and benign thyroid nodule(BTN)*** microbial signatures were identified in PTC,BTN,and their adjacent nontumor ***-thyroid tissue bacteria were verified by means of bacteria staining,fluorescence in situ hybridization,and immunoelectron *** found that 17 bacterial taxa were differentially abundant in PTC compared with BTN,which included enrichment in PTC of the pathobionts Rhodococcus,Neisseria,Streptococcus,Halomonas,and Devosia,and depletion of the beneficial bacteria *** differentially abundant bacteria could differentiate PTC tumor tissues(PTC-T)from BTN tissues(BTN-T)with an area under the curve(AUC)of 81.66%.Microbial network analysis showed increased correlation strengths among the bacterial taxa in PTC-T in comparison with ***-corresponding bacteria(i.e.,Erwinia,Bacillus,and Acinetobacter)were found to be enriched in PTC with Hashimoto’s ***,our integrative analysis revealed that the PTC-enriched bacteria had a positive association with key PTC-oncogenic pathway-related genes,including BRAF,KRAS,IRAK4,CTNNB1,PIK3CA,MAP3K7,and *** conclusion,our results suggest that intratumor bacteria dysbiosis is associated with the thyroid tumorigenesis and oncogenic signaling pathways of PTC.

关键词： Papillary thyroid cancer Benign thyroid nodule Bacteria Transcriptome Hashimoto’s thyroiditis

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非甾体类抗炎药引起的重度消化性溃疡与出血的内镜检查和药物治疗

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继续医学教育 2007年第3期21卷 1-6页

作者：沈祖尧香港中文大学消化疾病研究所

1 美国消化性溃疡的流行病学在美国，尽管单纯的消化性溃疡的发病率在下降，但是消化性溃疡合并出血的发病率及死亡率并没有太大变化。消化性溃疡合并出血的发病率为每年50000到100000例，并且每年约有150000消化性溃疡出血患者需要住... 详细信息

1 美国消化性溃疡的流行病学在美国，尽管单纯的消化性溃疡的发病率在下降，但是消化性溃疡合并出血的发病率及死亡率并没有太大变化。消化性溃疡合并出血的发病率为每年50000到100000例，并且每年约有150000消化性溃疡出血患者需要住院治疗，平均住院天数为5．7天。尽管内镜治疗法已有进步，再出血的发病率以及死亡率和过去三、四十年相比仍是基本持平，再出血发病率是15％～25％，死亡率是5％～12％（平均为10％）。最主要的原因是现在的病人群体年龄较高且并发症较多。

关键词：消化性溃疡合并出血非甾体类抗炎药药物治疗内镜检查平均住院天数发病率内镜治疗法死亡率

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CXCL10 plays a key role as an inflammatory mediator and a non-invasive biomarker of non-alcoholic steatohepatitis

CXCL10 plays a key role as an inflammatory mediator and a no...

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第九届全国免疫学学术大会

作者： Xiang Zhang jiayun Shen Kwan Man Eagle S.H.Chu Tung On yau joanne C.y.sung Minnie y.y.Go jun Deng Liwei Lu Vincent W.S.Wong joseph j.y.sung Geoffrey Farrell jun yu Institute of Digestive Disease and The Department of Medicine and Therapeutics State Key Laboratory of Digestive DiseaseThe Chinese University of Hong Kong

Perpetuate liver inflammation is crucial in the pathogenesis of non-alcoholic steatohepatitis(NASH).Expression of CXCL10,a pro-inflammatory cytokine that can recruit inflammatory cells to site of tissue damage,correlates positively with obesity and type 2 *** CXCL10 plays a role in NASH was *** we investigated the functional

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Aberrant cholesterol metabolic signaling impairs antitumor immunosurveillance through natural killer T cell dysfunction in obese liver

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Cellular & Molecular Immunology 2022年第7期19卷 834-847页

作者： Wenshu Tang jingying Zhou Weiqin yang yu Feng Haoran Wu Myth T.S.Mok Lingyun Zhang Zhixian Liang Xiaoyu Liu Zhewen Xiong Xuezhen Zeng jing Wang jiahuan Lu jingqing Li Hanyong Sun Xiaoyu Tian Philip Chun yeung yong Hou Heung Man Lee Candice C.H.Lam Howard H.W.Leung Anthony W.H.Chan Ka Fai To john Wong Paul B.S.Lai Kelvin K.C.Ng Simon K.H.Wong Vincent W.S.Wong Alice P.S.Kong joseph j.y.sung Alfred S.L.Cheng school of Biomedical Sciences The Chinese University of Hong KongHong Kong SAR 999077China Department of Anatomical and Cellular Pathology The Chinese University of Hong KongHong Kong SAR 999077China Department of Liver Surgery Ren Ji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127China Department of Surgery The Chinese University of Hong KongHong Kong SAR 999077China Department of Medicine and Therapeutics The Chinese University of Hong Kong.Hong Kong SAR 999077China State Key Laboratory of Digestive Disease The Chinese University of Hong KongHong Kong SAR 999077China Lee Kong Chian School of Medicine Nanyang Technological UniversitySingapore 639798Singapore Institute of Precision Medicine The First Affliated HospitalSun Yat-sen UniversityGuangzhou 510080China

Obesity is a major risk factor for cancers including hepatocellular carcinoma(HCC)that develops from a background of non-alcoholic fatty liver disease(NAFLD).Hypercholesterolemia is a common comorbidity of *** cholesterol biosynthesis mainly occurs in the liver,its role in HCC development of obese people remains *** high-fat high-carbohydrate diet-associated orthotopic and spontaneous NAFLD-HCC mouse models,we found that hepatic cholesterol accumulation in obesity selectively suppressed natural killer T(NKT)cell-mediated antitumor *** analysis of human liver revealed aberrant cholesterol metabolism and NKT cell dysfunction in NAFLD ***,cholesterol-lowering rosuvastatin restored NKT expansion and cytotoxicity to prevent obesogenic diet-promoted HCC ***,suppression of hepatic cholesterol biosynthesis by a mammalian target of rapamycin(mTOR)inhibitor vistusertib preceded tumor regression,which was abolished by NKT inactivation but not CD8^(+)T cell ***,sterol regulatory element-binding protein 2(SREBP2)-driven excessive cholesterol production from hepatocytes induced lipid peroxide accumulation and deficient cytotoxicity in NKT cells,which were supported by findings in people with obesity,NAFLD and *** study highlights mTORC1/SREBP2/cholesterol-mediated NKT dysfunction in the tumor-promoting NAFLD liver microenvironment,providing intervention strategies that invigorating NKT cells to control HCC in the obesity epidemic.

关键词： Cancer microenvironment Cancer models Tumour immunology

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消化道溃疡出血继续用低剂量阿司匹林

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中国全科医学 2010年第26期13卷 2915-2915页

作者： joseph j.y.sung james y.W.Lau jessica y.L.Ching 周淑新中国石油中心医院

背景对接受低剂量阿司匹林治疗发生消化道溃疡出血患者,采取内镜下止血治疗后是否应继续使用阿司匹林。目的检验内镜下控制溃疡出血后继续采用阿司匹林与质子泵抑制剂联合治疗并不劣于停用阿司匹林治疗,基于患心血管或脑血管病的成年复... 详细信息

背景对接受低剂量阿司匹林治疗发生消化道溃疡出血患者,采取内镜下止血治疗后是否应继续使用阿司匹林。目的检验内镜下控制溃疡出血后继续采用阿司匹林与质子泵抑制剂联合治疗并不劣于停用阿司匹林治疗,基于患心血管或脑血管病的成年复发性溃疡出血的患者。设计平行随机安慰剂对照试验。试验中患者和临床医生对指定的治疗双盲,从2003—2006年,将计算机生成的编号密封。设施一家三级内镜中心。患者接受低剂量阿司匹林的消化道溃疡出血者。干预78例患者接受阿司匹林80mg/d,78例接受安慰剂8周,立即采取内镜治疗。所有患者输注泮托拉唑(pantoprazole)治疗72h,接着口服泮托拉唑。对所有患者进行跟踪随访。测量初级终点为用内镜确定30日内再发溃疡出血。次极终点为8周内所有原因和特发原因所致的死亡率。结果 56例患者纳入治疗意向分析。3例在做跟踪前退出了试验。阿司匹林组30日内再发溃疡出血的发生率为10.3%,安慰剂组为5.4%〔相差4.9%,95%CI(3.6%～13.4%)〕,所有原因死亡率,接受阿司匹林治疗的患者比用安慰剂的患者要低〔1.3%vs12.9%;相差11.6%,95%CI(3.7%～19.5%)〕。与安慰剂组患者比较,将阿司匹林组患者死亡率较低的原因归因于心血管、脑血管或胃肠道并发症较少〔1.3%vs10.3%,相差9%,95%CI(1.7%～16.3%)〕。局限性样本量相对较小;仅做了低剂量阿司匹林80mg;安慰剂组有2例再发出血患者未做进一步内镜检查。结论接受低剂量阿司匹林治疗者发生消化道溃疡性出血后,继续用阿司匹林治疗可增加再发出血的风险,但有可能减少死亡率。证实这些发现还需做大量的实验研究。

关键词：消化道溃疡出血剂量阿司匹林内镜下止血治疗随机安慰剂出血患者质子泵抑制剂对照试验胃肠道并发症

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WONCA研究论文摘要汇编(八)——消化道溃疡出血继续用低剂量阿司匹林:随机试验

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中国全科医学 2010年第10期13卷 1108-1108页

作者： joseph j.y.sung james y.W.Lau jessica y.L.Ching 周淑新中国石油中心医院

背景未能确定,对接受低剂量阿司匹林出现了消化道溃疡出血病人,采取内镜下止血治疗后是否应继续用阿司匹林疗法。目的检测,内镜下控制溃疡出血后继续用阿司匹林与质子泵抑制剂并用并不劣于停用阿司匹林治疗,基于患心血管或脑血管病的成... 详细信息

背景未能确定,对接受低剂量阿司匹林出现了消化道溃疡出血病人,采取内镜下止血治疗后是否应继续用阿司匹林疗法。目的检测,内镜下控制溃疡出血后继续用阿司匹林与质子泵抑制剂并用并不劣于停用阿司匹林治疗,基于患心血管或脑血管病的成人复发性溃疡出血的观点。设计平行随机安慰剂对照非劣性试验。试验中病人和临床医生对指定治疗双盲,从2003—2006年,将计算机生成编号密封。[第一段]

关键词：消化道溃疡出血剂量阿司匹林随机试验 WONCA 论文摘要内镜下止血治疗汇编质子泵抑制剂

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Hemodynamic management of congenital diaphragmatic hernia:the role of targeted neonatal echocardiography

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World journal of Pediatric Surgery 2024年第2期7卷 81-89页

作者： Aimann Surak Linda Mahgoub joseph y Ting Department of Pediatrics University of AlbertaEdmontonAlbertaCanada

Congenital diaphragmatic hernia(CDH)is a major congenital anomaly,resulting from the herniation of abdominal contents into the thoracic cavity,thereby impeding the proper development of the lungs and pulmonary *** severity correlates with a spectrum of pulmonary hypoplasia,pulmonary hypertension(PHT),and cardiac dysfunction,constituting the pathophysiological triad of this complex *** accurate diagnosis and effective management of PHT and cardiac dysfunction is pivotal to optimizing patient *** neonatal echocardiography is instrumental in delivering real-time data crucial for the bespoke,pathophysiology-targeted hemodynamic management of CDH-associated PHT.

关键词： congenital targeted neonatal

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Serum ferritin and the risk of early-onset colorectal cancer

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World journal of Gastrointestinal Oncology 2024年第8期16卷 3496-3506页

作者： Adam L Urback Kylee Martens Hannah Stowe McMurry Emerson y Chen Caitlin Citti Anil Sharma Adel Kardosh joseph j Shatzel Division of Internal Medicine Oregon Health&Science UniversityPortlandOR 97239United States Division of Hematology and Medical Oncology Knight Cancer InstituteOregon Health&Science UniversityPortlandOR 97239United States Division of Gastroenterology&Hepatology Oregon Health&Science UniversityPortlandOR 97239United States

BACKGROUND The incidence of early-onset colorectal cancer(EO-CRC)is rising in the United States,and is often diagnosed at advanced *** serum ferritin is often incidentally discovered in young adults,however,the indication for endoscopy in EO-CRC is *** To compare serum ferritin between patients with EO-CRC and healthy controls(HCs),and examine the association of serum ferritin in EO-CRC with patient-and disease-specific *** A retrospective study of patientsyears with newly-diagnosed EO-CRC was conducted from 1/2013-12/*** were included if serum ferritin was measured within 2 years prior to 1 year following CRC histologic *** supplement the analysis,a cohort of HCs meeting similar inclusion and exclusion criteria were identified for comparison.A sensitivity analysis including only patients with serum ferritin obtained at or before diagnosis was separately performed to minimize risk of *** Among 85 patients identified with EO-CRC(48 females),the median serum ferritin level was 26 ng/mL(rangeyears(P=0.002 and Py).Stage IV disease was associated with significantly higher serum ferritin compared to less advanced stages(Py *** Severe iron deficiency may indicate an increased risk of EO-CRC,particularly at earlier *** studies defining the optimal serum ferritin threshold and routine incorporation of serum ferritin in screening algorithms is essential to develop more effective screening strategies for EO-CRC.

关键词： Early-onset young-onset Colorectal cancer Age Ferritin Iron deficiency

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Construction of 2D/2D Ti_(3)C_(2)T_(x)MXene/CdS heterojunction with photothermal effect for efficient photocatalytic hydrogen production

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journal of Materials Science & Technology 2024年第4期171卷 1-9页

作者： T.y.Huang Z.yang S.y.yang Z.H.Dai y.j.Liu j.H.Liao G.y.Zhong Z.j.Xie y.P.Fang S.S.Zhang Key Laboratory for Biobased Materials and Energy of Ministry of Education Guangdong Laboratory for Lingnan Modern AgricultureCollege of Materials and EnergySouth China Agricultural UniversityGuangzhou 510643China School of Physics and Optoelectronics South China University of TechnologyGuangzhou 510640China School of Chemical Engineering and Energy Technology Guangdong Provincial Key Laboratory of Distributed Energy SystemsDongguan University of TechnologyDongguan 523808China

The insensitivity of semiconductors to visible and infrared light is a key constraint on the utilization of light energy in photocatalytic *** photocatalysts with full-spectrum absorption through surface engineering is an effective approach to fully harnessing light energy in semiconductor ***,a novel stable Ti_(3)C_(2)T_(x)MXene/CdS heterojunction catalyst is obtained by in-situ epitaxial growth of two-dimensional(2D)CdS nanosheets on 2D MXene interface via a solvothermal *** exceptional light absorption properties of MXene confer outstanding full-spectrum driven photocat-alytic hydrogen evolution capability upon the heterogeneous *** unique 2D/2D structure effectively mitigated the recombination of photogenerated carriers,enhancing the photocatalytic performance of the ***,the composite catalyst exhibits a significantly higher surface temperature of 80.4℃under visible light irradiation at an intensity of 0.1 W/cm^(2),which is 1.84 times higher than that of *** irradiation of visible and near infrared light,the composite catalyst with photothermal ef-fect demonstrates a remarkable hydrogen evolution rate of 65.4 mmol g^(-1)h^(-1),which is 7.2 times higher than that of CdS *** study introduces a novel approach for constructing full-spectrum absorption catalysts and expands the application of the photothermal effect in photocatalytic hydrogen evolution research.

关键词： Two-dimensional structure Heterostructure Photothermal effect Photocatalyst Hydrogen evolution

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