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molecular isolation and identification of mycobacterium avium *** in Didelphis virginiana from Hidalgo,mexico

Asian Pacific Journal of Tropical medicine 2020年第3期13卷 131-136页

作者： Nallely Rivero-perez Juan Ocampo-López Benjamín Valladares-Carranza Fabián R.Gómez de Anda Francisco J.Pena Jiménez Victor m.martínez Juárez Armando Peláez Acero jose I.Olave Leyva deyanira Ojeda-Ramírez Adrian Zaragoza-Bastida Area Académica de medicina Veterinaria y Zootecnia Instituto de Ciencias AgropecuariaUniversidad Autónoma del Estado de HidalgoAv.Universidad Km 1Ex-Hda.de AquetzalpaC.P.43600 TulancingoHidalgoMéxico Centro de Investigación y Estudios Avanzados en Salud Animal Facultad de Medicina Veterinaria y ZootecniaUniversidad Autónoma del Estado de MéxicoKm 15.5 Carretera Panamericana Toluca-AtlacomulcoC.P.50200 TolucaEstado de MéxicoMéxico Centro Universitario del Sur/División de Ciencias de la Salud/departamento de Ciencias Clínicas/Hospital Veterinario CUSUR Universidad de Guadalajara Av.Enrique Arreola Silva No.883colonia centro C.P.49000Ciudad GuzmánJaliscoMéxico

Objective:To isolate and identify the exact species of the genus mycobacterium from Didelphis(D.)virginiana,and the direct implications of this bacterium to public health and veterinary ***:Thirty-one *** were captured and necropsied in Hidalgo,*** samples were collected to culture mycobacteria present and examine individual specimens’*** identification was obtained through the application of amplification and sequencing of 16S rDNA ***:Three strains were isolated and identified as mycobacterium(m.)avium *** by utilizing *** complexspecific *** lesions were observed in the subpleural zone(granuloma gradeⅡ)and bronchial(granuloma gradeⅠ)of the lungs of *** with positive ***:Three strains of *** ***,from lung tissue samples of *** were *** subspecies of *** has important implications in public health and veterinary medicine.

关键词： Didelphis virginiana mycobacterium avium complex mycobacterium avium subsp.hominissuis Public health

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Antinociceptive and Anti-inflammatory Activities of methanol Extract of Ormosia coccinea （Aubl,） Jacks in vivo

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Journal of Pharmacy and Pharmacology 2017年第6期5卷 289-298页

作者： miguel Angel Diaz C. Lorena H. Cruz m. mahabir P. Gupta Dionisio Olmedo jose Luis Lopez-perez Estela Guerrero de Leon Juan Antonio moran-Pinzon Direccidn Nacional de Farmacia y Drogas Ministerio de Salud Panama Apartado 06812-0816 Republica de Panama Centro de lnvestigaciones Farmacognosticas de la Flora Panamena (CIFLORPAN) Facultad de Farmacia Universidad de Panama Panama Apartado 0824-00172 Republica de Panama departamento de Quimica FarmacOutica Facultad de Farmacia Universidad de Salamanca Salamanca Apartado 37007 Spain departamento de Farmacologla Facultad de Medicina Universidad de Panama Panama Apartado 0824 Panama

The present study was conducted to evaluate the antinociceptive and anti-inflammatory activities of methanol extract of rachis of Ormosia coccinea （Aubl.） Jacks （mEOC） using animal models of nociception and inflammation. The antinociceptive activity of the extract was assessed using acetic acid-induced abdominal writhing, hot-plate, and formalin tests. Oral administration of mEOC （500 mg/kg） produced significant （t7 〈 0.05） antinociceptive effects when tested in mice using acetic acid-induced abdominal writhing test and on the inflammatory phase of the formalin test. It was also demonstrated that mEOC had no significant effect on the response latency time to the heat stimulus in the thermal model of the hot plate test. The anti-inflammatory activity of the extract was assessed using carrageenan, histamine and serotonin induced oedema in rat paw. The oral administration of mEOC showed maximum inhibition （64.29%） at 1 h on carrageenan edema, but it did not modify the edema induced by histamine and serotonin. The present results suggest that mEOC has a peripheral antinoeiceptive and anti-inflammatory action.

关键词： Ormosia coccinea antinociceptive anti-inflammatory fabaceae.

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Preoperative calculation of angles of vision and working area in laparoscopic surgery to treat a giant hiatal hernia

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World Journal of Gastrointestinal Surgery 2021年第12期13卷 1638-1650页

作者： Francisco Javier perez lara Rogelio Zubizarreta Jimenez Francisco Javier moya Donoso jose manuel Hernández Gonzalez Tatiana Prieto-Puga Arjona Arturo del Rey moreno maria Pitarch martinez Surgery Service Antequera HospitalRincón de la Victoria 29730MálagaSpain DOXA microfluidics Rincon de la Victoria 29738MálagaSpain

BACKGROUND Giant hiatal hernias still pose a major challenge to digestive surgeons,and their repair is sometimes a highly complex *** is usually performed by laparoscopy,while the role of the thoracoscopic approach has yet to be clearly *** To preoperatively detect patients with a giant hiatal hernia in whom it would not be safe to perform laparoscopic surgery and who,therefore,would be candidates for a thoracoscopic *** In the present study,using imaging test we preoperatively simulate the field of vision of the camera and the working area(instrumental access)that can be obtained in each patient when the laparoscopic approach is *** From data obtained,we can calculate the access angles that will be obtained in a preoperative computerised axial tomography coronal section,according to the location of the *** also provide the formula for performing the angle calculations If the trocars are placed in loss common situations,thus enabling us to determine the visibility and manoeuvrability for any position of the *** The working area determines the cases in which we can operate safely and those in which certain areas of the hernia cannot be accessed,which is when the thoracoscopic approach would be safer.

关键词： Hiatal hernia Angles Approach laparoscopy Thoracoscopy

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Preparation, characterization and gastrointestinal stability of silk fibroin nanoparticles loaded with red wine polyphenols

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Food Bioscience 2023年第2期52卷 1091-1101页

作者： Diego Paladines-Quezada Carolina Cueva Rocio Gil-munoz jose Luis Cenis Begona Bartolome m.Victoria moreno-Arribas Antonio Abel Lozano-perez Equipo de Enologia y Viticultura Estacion Enologica de JumillaInstituto Murciano de Investigacion y Desarrollo Agrario y Medioambiental(IMIDA)JumillaMurcia30520Spain Instituto de Investigacion en Ciencias de la Alimentacion(CIAL) CSIC-UAMC/Nicolas Cabrera 928049MadridSpain departamento de Biotecnologia Genomica y Mejora VegetalInstituto Murciano de Investigacion y Desarrollo Agrario y Medioambiental(IMIDA)La AlbercaMurcia30150Spain Instituto murciano de Investigacion Biosanitaria(ImIB) 30120El PalmarMurciaSpain

Silk fibroin nanoparticles(SFN)are interesting drug delivery systems due to their high load capacity and low *** aim of this study was to reveal how SFN load red wine polyphenols(RWP),and to assess their protective effect during the early stages of digestion by using the dynamic gastrointestinal simulator(simgi^(R))under physiological ***-SFN were prepared by incubation of SFN in red wine and recovered by *** obtained nanoparticles(particle size of 194.4±2.1 nm and a Zeta potential of􀀀22.7±0.1 mV)showed a significant polyphenol loading content(7386.59±1018.28μg/g)with a high encapsulation efficiency(66.66±2.58%).They were highly stable during their passage through the digestive tract,being the activity of RWP highly *** data paves the way for future developments of SFN as stabilizers with a wide range of applications in the field of food additives.

关键词： Silk fibroin nanoparticles Wine polyphenols Adsorption Dynamic gastrointestinal simulator(simgi^(R)) Protein digestion

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Virulence markers in Staphylococcus aureus Strains Isolated from Hemodialysis Catheters of mexican Patients

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Advances in microbiology 2012年第4期2卷 476-487页

作者： Gloria Paniagua-Contreras Teresita Sainz-Espunes Eric monroy-perez jose Raymundo Rodriguez-moctezuma Diego Arenas-Aranda Erasmo Negrete-Abascal Sergio Vaca FES-Iztacala Universidad Nacional Autonoma de Mexico(UNAM)TlalnepantlaMéxico Ciencias Biológicas y de la Salud Universidad Autonoma Metropolitana(UAM)—XochimilcoCalzada del HuesoMéxico departamento de Sistemas Biológicos División de Ciencias Biológicas y de la SaludUniversidad Autonoma Metropolitana(UAM)—XochimilcoCalzada del HuesoMéxico Hospital General Regional 72 Instituto Mexicano del Seguro SocialGustavo Baz Esquina F.GómezTlalnepantlaMéxico

methicillin-resistant Staphylococcus aureus is an increasingly important cause of nosocomial bacterial infections worldwide. S. aureus is responsible for several pathologies, including skin infections, endocarditis, meningitis, deep-tissue ulcers, and sepsis. S. aureus biofilm formation on catheters and other medical devices is a major post-operative concern, because biofilms are often the source of persistent and difficult to treat bacterial infections. While catheter-related S. aureus infections have been reported, the strains responsible for these infections have not been genetically characterized. We genetically characterized S. aureus strains isolated from hemodialysis catheters in mexican patients. The frequency of 35 genes coding for adhesins, toxins, and other virulence-associated products in the 55 isolated S. aureus strains was determined using PCR, while real-time PCR was used to examine the level of gene expression. Of the 55 S. aureus strains isolated from 109 patients, 45 (81.8%) were determined to be methicillin-resistant. The icaA, rbf, sarA, and agr genes are involved in biofilm formation and bacterial dispersion and were detected in 96.3%, 40.0%, 74.5%, and 100% of S. aureus strains, respectively, and 70.9% of the strains formed a detectable biofilm. Interestingly, 67.2% of the strains contained the icaA, agr, spa, clfA, sdrC, sdrD, sdrE, seg, seh, and sei genes, suggesting that this gene combination is important for successful catheter colonization. The results of this study provide significant insight into the virulence gene make-up of catheter-colonizing S. aureus strains, and will assist in developing a more targeted treatment approach for persistent S. aureus biofilm contamination of medical devices.

关键词： S.aureus Hemodialysis Catheter Genotyping

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Synthesis and Characterization of a Polymeric material Blended to Bone Forming Elements

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Journal of Biosciences and medicines 2023年第9期11卷 181-194页

作者： Esther perez-Torrero Leticia Esmeralda Luna-Rodriguez Gerardo Antonio Fonseca-Hernandez jose Santos-Cruz Eric mauricio Rivera-muñoz maria Lucero Gomez-Herrera Facultad de Ingeniería Investigación y Posgrado Campus Aeropuerto Universidad Autónomade Querétaro México Centro de Física Aplicada y Tecnología Avanzada (CFATA) de la Universidad Nacional Autónoma de méxico Facultad de Química Investigación y Posgrado Campus Cerro de las Campanas UniversidadAutónoma de Querétaro México

A synthetizing material blended with two distinct proteins (collagen and casein) and mineral mixture, was developed in order to evaluate their properties suitable for possible applications in the biomedical such as inducing the regeneration of damaged bone, either due to an accident or illness. Samples were evaluated by 1) mechanical properties tests under the bending, 2) Scanning electronic microscopy and 3) Infrared spectroscopy were carried out. The results showed that the developed material has breaking strength and structure characteristics associated with the protein used in their composition. This fact suggests that the used protein determines the resistance of the material, in such a way according to the required use, being able to choose appropriate strength and duration either short or long time. The material composition for specific use, in order to find the most suitable mixture for bone replacement, or induce bone recovery, according to the required properties similar to those of damaged living tissue.

关键词： minerals Bone Polymethylmethacrylate Casein Collagen mechanical Properties Infrared Scanning Electronic microscopy

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Comparative study of breast cancer in mexican and mexican-American women

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Health 2010年第9期2卷 1040-1048页

作者： maria Elena martinez Luis Enrique Gutierrez-millan melissa Bondy Adrian Daneri-Navarro maria mercedes meza-montenegro Ivan Anduro-Corona ma Isabel Aramburo-Rubio Luz maria Adriana Balderas-Pena jose Adelfo Barragan-Ruiz Abenaa Brewster Graciela Caire-Juvera Juan manuel Castro-Cervantes mario Alberto Chavez Zamudio Giovanna Cruz Alicia del Toro-Arreola mary EEdgerton maria Rosa Flores-marquez Ramon Antonio Franco-Topete Helga Garcia Susan Andrea Gutierrez-Rubio Karin Hahn Luz margarita Jimenez-perez Ian KKomenaka Zoila Areli Lopez Bujanda Dihui Lu Gilberto morgan-Villela James Lmurray Jesse NNodora Antonio Oceguera-Villanueva miguel Angel Ortiz martinez laura perez michel Antonio Quintero-Ramos Aysegul Sahin Jeong Yun Shim maureen Stewart Gonzalo Vazquez-Camacho Betsy Wertheim Rachel Zenuk Patricia Thompson Arizona Cancer Center and mel and Enid Zuckerman College of Public Health University of ArizonaTucsonUSA departamento de Investigaciones Cientificas y Tecnologicas Universidad de SonoraHermosilloMexico University of Texas m.D.Anderson Cancer Center HoustonUSA departamento de Fisiologia Centro Universitario de Ciencias de la SaludUniversidad de GuadalajaraGuadalajaraMexico Instituto Tecnologico de Sonora Ciudad ObregónMexico Instituto mexicano del Seguro Social HermosilloMexico Instituto mexicano del Seguro Social CMNOGuadalajaraJalisco Centro de Investigacion en Alimentacion y desarrollo HermosilloMexico Instituto mexicano del Seguro Social Ciudad ObregonMexico OPD Hospital Civil de Guadalajara GuadalajaraMexico departamento de Salud Publica Centro Universitario de Ciencias de la SaludUniversidad de GuadalajaraGuadalajaraMexico maricopa medical Center Department of SurgeryPhoenixUSA University of North Carolina Lineberger Cancer CenterChapel HillUSA Arizona Cancer Center and department of Family and Community medicine University of ArizonaTucsonUSA Instituto Jalisciense de Cancerologia GuadalajaraMexico

Breast cancer is the number one cause of can- cer deaths among Hispanic women in the United States, and in mexico, it recently became the primary cause of cancer deaths. This malign- nancy represents a poorly understood and un- derstudied disease in Hispanic women. The ELla Binational Breast Cancer Study was es- tablished in 2006 as a multi-center study to as- sess patterns of breast tumor markers, clinical characteristics, and their risk factors in women of mexican descent. We describe the design and implementation of the ELla Study and provide a risk factor comparison between women in the U.S. and those in mexico based on a sample of 765 patients (364 in the U.S. and 401 in mexico). Compared to women in mexico, U.S. women had significantly (p mean live births) and breastfeeding rates (57.5% vs. 80.5%), higher use of oral contraceptives (60.7% vs. 50.1%) and hormone replacement therapy (23.3% vs. 7.6%), and higher family history of breast cancer (15.7% vs. 9.0%). Re- sults show that differences in breast cancer risk factor patterns exist between mexico and U.S. women. We provide lessons learned from the conduct of our study. Binational studies are an important step in understanding disease pat- terns and etiology for women in both countries.

关键词： Binational Study Breast Cancer Hispanics mexico United States

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