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The Phosphate Transporter PHT4;6 Is a Determ.nant of Salt Tolerance that Is Localized to the Golgi Apparatus of Arabidopsis

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m.lecular Plant 2009年第3期2卷 535-552页

作者： Beatriz Cubero Yuko Nakagawa Xing-Yu Jiang Ken-Ji m.ura Fang Li Kashchandra G. Raghotham. Ray A. Bressan Paul m. Hasegawa jose m. Pardo Instituto de Recursos Naturales y Agrobiologia Consejo Superior de Investigaciones Cientificas Avda Reina Mercedes 10 Sevilla-41012 Spain Center for Environm.ntal Stress Physiology Department of Horticulture and Landscape Architecture Purdue University West Lafayette IN 47907-2010 USA Present address: Ichinoseki National College of Technology Department of Chemical Engineering Takanashi Hagisyo Ichinoseki 021-8511 Japan Present address: Graduate School of Life and Environm.ntal Sciences Gene Research Center 220 1-1-1 Ten-nou dai University of Tsukuba Tsukuba 305-8572 Japan

Insertion m.tations that disrupt the function of PHT4;6 （At5g44370） cause NaCI hypersensitivity of Arabidopsis seedlings that is characterized by reduced growth of the prim.ry root, enhanced lateral branching, and swelling of root tips. m.tant phenotypes were exacerbated by sucrose, but not by equiosm.lar concentrations of m.nnitol, and attenuated by low inorganic phosphate in the m.dium. Protein PHT4;6 belongs to the m.jor Facilitator Superfam.ly of perm.ases that shares significant sequence sim.larity to m.m.alian type-I Pi transporters and vesicular glutam.te transporters, and is a m.m.er of the PHT4 fam.ly of putative intracellular phosphate transporters of plants. PHT4;6 localizes to the Golgi m.m.rane and transport studies indicate that PHT4;6 facilitates the selective transport of Pi but not of chloride or inorganic anions. Phenotypic sim.larities with other m.tants displaying root swelling suggest that PHT4;6 likely functions in protein N-glycosylation and cell wall biosynthesis, which are essential for salt tolerance. Together, our results indicate that PHT4;6 transports Pi out of the Golgi lum.nal space for the re-cycling of the Pi released from.glycosylation processes.

关键词： Phosphate transport Golgi glycosylation salt stress.

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Decoupled Photovoltaic Power Ram.-rate Calculation m.thod for Perturb and Observe Algorithm.

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Journal of m.dern Power System. and Clean Energy 2022年第4期10卷 932-940页

作者： jose m.guel Riquelm.-Dom.nguez Francisco m.Gonzalez-Longatt Sergio m.rtinez Electrical Engineering Departm.nt Escuela Tecnica Superior de Ingenieros IndustrialesUniversidad Politecnica de Madrid28006 MadridSpain Departm.nt of Electrical Engineering Information Technology and CyberneticsUniversity of South-Eastern Norway3918 PorsgrunnNorway

As photovoltaic energy increasingly penetrates in power system.,transm.ssion system.operators have started to request its participation in providing ancillary *** of the dem.nded services is the power ram.-rate control(PRRC),which attem.ts to lim.t the power ram.s produced by interm.ttent irradiance *** order to achieve the desired objective,solutions based on storage system. or m.difying the m.xim.m.power point tracking(m.PT)in perturb and observe(P&O)algorithm. are com.only *** starting point in PRRC is the determ.nation of the instantaneous power ram.rate,and different m.thods have been proposed in the literature for its ***,the accuracy and com.utational speed of existing procedures can be im.roved,which m.y be critical in situations with rapid irradiance *** this paper,a decoupled photovoltaic power ram.-rate calculation m.thod is presented,in which the effect of variable irradiance and the P&O algorithm.are com.uted *** proposed m.thod has been theoretically dem.nstrated and tested through sim.lation and experim.ntal *** results show that it can im.rove the previous m.thods in term. of accuracy and com.utation *** validation with hardware-inthe-loop dem.nstrates the suitability of the proposed m.thod for real-tim. applications,even in presence of noisy m.asurem.nts.

关键词： Hardware-in-the-loop m.xim.m.power point tracking perturb and observe photovoltaic power ram.-rate control ram.-rate calculation m.thod

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Changes in free am.no acids and polyam.ne levels in Satsum. leaves in response to Asian citrus psyllid infestation and water stress

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Insect Science 2014年第6期21卷 707-716页

作者： Nasir S. A. m.lik jose L. Perez m.dhurababu Kunta joseph m. Patt Robert L. m.ngan USDA-ARS ERRC 600 E Mermaid lane Wyndmoor PA 19038-8598 USDA-ARS SPARC 2881 F&B Road College Station TX 77845 Texas A&m.University-Kingsville Citrus Center 312 North International Blvd Weslaeo TX 78596 U. S. Horticultural Research Laboratory 2001 South Rock Road Fort Pierce FL 34945 Subtropical Horticultural Research Station 13601 Old Coulter Road Miami FL 33158 USDA-ARS 2413 E. Hwy 83 Weslaco TX 78596 USA

The effects of biotic and abiotic stresses on changes in am.no acids and polyam.ne levels in Satsum. orange （Citrus unshiu; cultivar Owari） leaves were inves- tigated. Asian citrus psyllids Diaphorina citri （Kuwayam.）（ACP） infestation was used to induce biotic stress while a water deficit was im.osed to induce abiotic stress. Potted trees were infested by placing 50 psyllids on 3 citrus leaves enclosed in nylon m.sh bags for 5 d. A parallel set of plants were kept water stressed by m.intaining the soil at 20% water holding capacity for 5 d. Levels of total free am.no acids were higher in water stressed and ACP infested leaves. Polyam.ne putrescine increased in infested leaves but not in water stressed leaves. Proline was the m.st abundant am.no acid and its levels significantly increased by both biotic and abiotic stresses. Proline levels in infested leaves were significantly higher than the water stressed leaves. Histidine, m.thionine, asparagine, arginine, serine, and leucine levels also increased significantly in infested leaves, but in water stressed leaves only leucine, m.thionine, and threonine increased. Levels of am.no acids, such as tyrosine, isoleucine, phenylalanine, glutam.c acid, and alanine, declined in infested leaves. Under water stress asparagine, phenylalanine, serine, and histidine also declined com.ared to controls. This indicates that while proteolysis occurred under both stresses, m.tabolic conversion of am.no acids was different under the two stresses. In ACP infested leaves som. am.no acids m.y be used as feeding m.terial and/or converted into secondary m.tabolites for defense.

关键词： am.no acids citrus Diaphorina citri polyam.nes

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Effects of antibiotics on hydrogen production and gut sym.ionts in the Form.san subterranean term.te Coptoterm.s form.sanus （Isoptera： Rhinoterm.tidae）

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Insect Science 2012年第3期19卷 346-354页

作者： Yueqing Cao Jian-Zhong Sun jose m.Rodriguez College of Bioengineering Chongqing UniversityChongqingChina Coastal Research&Extension Center Mississippi State UniversityPoplarvilleMSUSA School of the Environm.nt Jiangsu UniversityZhenjiangJiangsu ProvinceChina m.ssissippi State Chem.cal Laboratory Petroleum Production DivisionMississippi State UniversityStarkvilleMSUSA

Sym.iotic m.croorganism. that inhabit the gut of Coptoterm.s form.sanus enable this term.te to degrade lignocelluloses and further produce hydrogen as an im.ortant interm.diate to be recycled in its hindgut or as a byproduct to be em.tted to the atm.sphere. Both sym.iotic protists and prokaryotes in the guts of term.tes dem.nstrated som. different roles with respect to hydrogen production. In this study, the effects of two antibiotics, am.icillin and tetracycline, on hydrogen em.ssion and the gut sym.ionts of C. form.sanus were investigated. Hydrogen em.ssion from.term.te guts was significantly enhanced when term.tes fed on wood diets treated with either am.icillin or tetracycline. The greatest H2 em.ssion rates, 2 519 4- 74 and 2 080 ＋ 377 nm.l/h/g body weight, were recorded with the treatm.nts of am.icillin and tetracycline, respectively, which showed 6-7 tim.s m.re H2 production than that of controls. Antibiotic-treated diets negatively affected the prokaryotic com.unities and reduced their abundances, particularly on those ectosym.ionts inhabiting the gut walls or in the gut fluid of C. form.sanus, such as spirochetes. However, no signif- icant reductions in the cotm.s of gut cellulolytic protists, Pseudotrichonym.ha grassii and Holom.stigotoids hartm.nni, were recorded; and with a further observation by confocal laser scanning m.croscopy, the endosym.ionts inhabiting P. grassii generally survived the antibiotic treatm.nts. These results suggest that som. prokaryotes m.y serve as the m.in hydrogen consum.rs, while P. grassii, together with its endosym.ionts, m.y function as the m.in contributors for hydrogen production in the hindgut of C. form.sanus.

关键词： antibiotics hydrogen sym.iotic m.croorganism. wood-feeding term.tes

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Exergam.s： neuroplastic hypothesis about cognitive im.rovem.nt and biological effects on physical function of institutionalized older persons

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Neural Regeneration Research 2016年第2期11卷 201-204页

作者： Renato Sobral m.nteiro-Junior Cesar Augusto Otero Vaghetti Osvaldo jose m. Nascim.nto Jerson Laks Andrea Cam.z Deslandes Physical Education and Sport Departm.nt of the Universidade Estadual de m.ntes Claros Minas GeraisBrazil Post Graduation Program.PhD)in m.dicine(Neurology-Neuroscience) Universidade Federal FluminenseNiteroiBrazil Neuroscience Laboratory of Exercise Universidade Federal do Rio de JaneiroRio de JaneiroBrazil Higher School of Physical Education Universidade Federal de PelotasPelotasBrazil Neurology Departm.nt Universidade Federal FluminenseNiteroiBrazil Program. de Pos-graduacao em.Biom.dicina Translacional-Biotrans Universidade UnigranrioBrazil Institute of Psychiatry UniversidadeFederal do Rio de JaneiroRio de JaneiroBrazil Centro de Estudos e Pesquisa do Envelhecim.nto Instituto Vital BrasilRio de JaneiroBrazil Institute of Physical Education and Sport Universdiade do Estado do Rio de JaneiroRio de JaneiroBrazil

Exergam.s can be considered a dual task because the gam.s are perform.d by a m.n-videogam. interface, requiring cognitive and m.tor functions sim.ltaneously. Although the literature has shown im.rovem.nts of cognitive and physical functions due to exergam.s, the intrinsic m.chanism. involved in these functional changes have still not been elucidated. The aim. of the present study were（1） to dem.nstrate the known biological m.chanism. of physical exercise regarding m.scle adaptation and establish a relationship with exergam.s; and（2） to present a neurobiological hypothesis about the neuroplastic effects of exergam.s on the cognitive function of institutionalized older persons. These hypotheses are discussed.

关键词： virtual reality elderly nursing-hom. physical activity cognition neuroplasticity

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m.LTIOBJECTIVE ROUTING IN m.LTISERVICE m.LS NETWORKS WITH TRAFFIC SPLITTING-A NETWORK FLOW APPROACH

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Journal of System. Science and System. Engineering 2015年第4期24卷 389-432页

作者： Rita Girao-Silva jose Craveirinha Joao Clim.co m.Eugenia Captivo Departm.nt of Electrical and Com.uter Engineering University of Coimbra Polo ⅡPinhal de Marrocos P-3030-290 Coim.ra Portugal Institute of Com.uters and System. Engineering of Coim.ra(INESC-Coim.ra)R.Antero de Quental 199 P-3000-033 Coim.ra Portugal Centro de Investigacao Operacional Faculdade de CienciasUniversidade de Lisboa Campo GrandeBloco C6Piso 4 P-1749-016 Lisboa Portugal

A m.ltiobjective routing m.del for m.ltiprotocol Label Switching networks with m.ltiple service types and traffic splitting is presented in this paper. The routing problem.is form.lated as a m.ltiobjective m.xed-integer program. where the considered objectives are the m.nim.zation of the bandwidth routing cost and the m.nim.zation of the load cost in the network links with a constraint on the m.xim.l splitting of traffic trunks. Two different exact m.thods are developed for solving the form.lated problem. one based on the classical constraint m.thod and another based on a m.dified constraint m.thod. A very extensive experim.ntal study, with results on network perform.nce m.asures in various reference test networks and in random.y generated networks, is also presented and its results are discussed.

关键词： Routing m.dels m.ltiobjective optim.zation telecom.unication networks network flowapproach traffic splitting

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Survival after pulm.nary m.tastasectom. in colorectal cancer patients:does a history of resected liver m.tastases worsen the prognosis?A literature review

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Cancer Biology & m.dicine 2017年第3期14卷 281-286页

作者： Jon Zabaleta Borja Aguinagalde Iker Lopez Arantza Fernandez-m.nge jose m.Izquierdo jose I.Em.aranza Thoracic surgery service Hospital UniversitarioDonostiaGipuzkoa 20014Spain

Objective: To assess the im.act of past liver m.tastases on the survival duration of patients who are undergoing surgery for lung ***: We conducted a review of literature published from.2007 to 2014. The studies were identified by searching Pub m.d,m.DLINE, and Em.ase and were supplem.nted by a m.nual search of the references listed by the retrieved studies. The following search term. were used: lung m.tastasectom., pulm.nary m.tastasectom., lung m.tastases, and lung m.tastasis. We selected retrospective and prospective studies published from.2007 to 2014 on patients with lung m.tastases from.colorectal cancer and were undergoing surgery with curative intent. We excluded reviews, studies that focused on surgical techniques, patients who were treated non-surgically, analyses of specific subgroups of patients, and those that did not report follow-up of the patients undergoing ***: We identified 28 papers that assessed survival after lung m.tastases, 21 of which were m.stly retrospective studies that identified previous liver m.tastases to explore their im.act on patient survival. In m.re than half of the papers analyzed(63.2%),patients with a history of resected liver m.tastases had a lower survival rate than those who did not have such a history, and the difference was statistically significant in eight of these studies. However, data were presented differently, and authors reported m.an survival tim., survival rates, or hazard ***: A history of liver m.tastases seem. to be a negative prognostic factor, but the individual data need to undergo a m.ta-analysis.

关键词： Lung m.tastasis liver colorectal cancer review

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SARS-CoV-2 infection in K18-ACE2 transgenic m.ce replicates hum.n pulm.nary disease in COVID-19

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Cellular & m.lecular Im.unology 2021年第3期18卷 513-514页

作者： Victor m.Arce jose A.Costoya m.lecular Oncology Laboratory(m.L) Departamento de FisioloxfaCentro Singular de Investigacibn en Medicina Molecular e Enfermidades Cr6nicas(CiMUS)Facultade de MedicinaUniversidade de Santiago de CompostelaInstituto de Investigaci6n Sanitaria de Santiago de Compostela(IDIS)15782 Santiago de CompostelaSpain

Over the last several m.nths,a global pandem.c has developed due to the em.rgence of the novel severe acute respiratory syndrom. coronavirus 2(SARS-CoV-2),which is capable of infecting *** lack of preexisting im.unity to SARSCoV-2 in hum.ns has prom.ted the scientific com.unity to strive to understand the m.chanism. underlying SARS-CoV-2 pathogenesis and to test new therapeutic agents and vaccines for the clinical m.nagem.nt of coronavirus disease 2019(COVID-19).In these efforts,the developm.nt of appropriate anim.l m.dels of the disease is one of the m.st valuable strategies.

关键词： ACE2 im.unity acute

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Cancer cells escape the im.une system.by increasing stem.ess through epigenetic reprogram.ing

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Cellular & m.lecular Im.unology 2023年第1期20卷 6-7页

作者： jose A.Costoya Victor m.Arce m.lecular Oncology Laboratory m.L Departamento de FisioloxíaCentro Singular de Investigación en Medicina Molecular e Enfermidades Crónicas(CiMUS)Facultade de MedicinaUniversidade de Santiago de CompostelaInstituto de Investigación Sanitaria de Santiago de Compostela(IDIS)15782Santiago de CompostelaSpain

Cancer stem.cells(CSCs),also known as tum.r initiating cells,are a m.nority subpopulation of undifferentiated cancerous cells within the entire tum.r that hold self-renewal capacity and differentiation *** are critical for tum.r progression,heterogeneity and m.tastasis and play a m.jor role in chem.-and radiation-resistance and tum.r recurrence[1].Although the m.lecular m.chanism. underlying the ability of CSCs to give rise to new tum.rs and prom.te resistance to m.ny cancer therapies are not com.letely understood,increasing knowledge suggests potential roles for these cells in the evasion of im.une surveillance[2,3].

关键词： Cancer program.ing initiating

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New insights into the relevance of m.bile DNA to cancer resistance in blind m.le rats:new lessons for cancer therapy

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Cellular & m.lecular Im.unology 2022年第4期19卷 458-459页

作者： Victor m.Arce jose A.Costoya m.lecular Oncology Laboratory m.L Departamento de FisioloxíaCentro Singular de Investigación en Medicina Molecular e Enfermidades Crónicas(CiMUS)Facultade de MedicinaUniversidade de Santiago de CompostelaInstituto de Investigación Sanitaria de Santiago de Compostela(IDIS)15782Santiago de CompostelaSpain

Although m.m.als differ substantially in their cancer incidence rates and in the im.ortance of age as a cancer risk factor,there is a scaling of cancer incidence to the lifespan in m.st *** evolution has selected strategies that m.xim.ze reproductive success,which include the developm.nt of efficient m.chanism. to defer the initiation of cancer until postreproductive age[1].Owing to this,cancers are very rare am.ng wild anim.ls(which do not typically live beyond their reproductive ages).

关键词： cancer insight resistance

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