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Current status and potential of morphometric sperm analysis

Asian Journal of Andrology 2016年第6期18卷 863-870页

作者： Alejandro Maroto-Morales Olga Garcia-Alvarez Manuel Ramon Felipe Martinez-Pastor M Rocio Fernandez-Santos A josefa Soler jose julian garde SaBio IREC (CSIC - UCLM -JCCM) Albacete Spain Biomedical Center Medical Faculty in Pilsen Charles University in Prague Pilsen Czech Republic Regional Center of Animal Selection and Reproduction (CERSYRA) JCCM Valdepenas Spain 4Institute for Animal Health and Cattle Development University of Leon LeOn Spain Department of Molecular Biology University of Leon Leon Spain Faculty of Pharmacy (UCLM) Albacete Spain

The spermatozoon is the most diverse cell type known and this diversity is considered to reflect differences in sperm function. How the diversity in sperm morphology arose during speciation and what role the different specializations play in sperm function, however, remain incompletely characterized. This work reviews the hypotheses proposed to explain sperm morphological evolution, with a focus on some aspects of sperm morphometric evaluation; the ability of morphometrics to predict sperm cryoresistance and male fertility is also discussed. For this, the evaluation of patterns of change of sperm head morphometry throughout a process, instead of the study of the morphometric characteristics of the sperm head at different stages, allows a better identification of the males with different sperm cryoconservation ability. These new approaches, together with more studies employing a greater number of individuals, are needed to obtain novel results concerning the role of sperm morphometry on sperm function. Future studies should aim at understanding the causes of sperm design diversity and the mechanisms that generate them, giving increased attention to other sperm structures besides the sperm head. The implementation of scientific and technological advances could benefit the simultaneous examination of sperm phenotype and sperm function, demonstrating that sperm morphometry could be a useful tool for sperm assessment.

关键词： computer-assisted sperm morphometric analysis mammals sperm function sperm morphometry

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Minimizing sperm oxidative stress using nanotechnology for breeding programs in rams

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Journal of Animal Science and Biotechnology 2023年第6期14卷 2395-2410页

作者： Alejandro Jurado‑Campos Pedro Javier Soria‑Meneses Maria Arenas‑Moreira Carlos Alonso‑Moreno Virginia Rodriguez‑Robledo Ana josefa Soler jose julian garde Maria del Rocio Fernandez‑Santos SaBio IREC(CSIC-UCLM-JCCM) Campus UniversitarioS/N02071 AlbaceteSpain. Departamento de Quimica Inorganica Organica Y Bioquimica‑Centro de Innovacion en Quimica Avanzada(ORFEO‑CINQA)Facultad de FarmaciaUniversidad de Castilla-La Mancha02008 AlbaceteSpain. Centro Regional de Investigacion Biomedicas Unidad nanoDrugUniversidad de Castilla-La Mancha02008 AlbaceteSpain. Facultad de Farmacia Universidad de Castilla La Mancha02071 AlbaceteSpain.

Background Artificial insemination(AI)is a routine breeding technology in animal ***,the temperature-sensitive nature and short fertile lifespan of ram sperm samples hamper its use in *** this sense,nanotechnology is an interesting tool to improve sperm protection due to the development of nanomaterials for AI,which could be used as delivery *** this work,we explored the feasibility of vitamin E nanoemulsion(NE)for improving sperm quality during *** With the aim of evaluating this proposal,ejaculates of 7 mature rams of Manchega breed were collected by artificial vagina and extended to 60×10^(6)spz/mL in *** containing control and NE(12 mmol/L)with and without exogenous oxidative stress(100μmol/L Fe2+/ascorbate)were stored at 22 and 15℃and motility(CASA),viability(YO-PRO/PI),acrosomal integrity(PNA-FITC/PI),mitochondrial membrane potential(Mitotracker Deep Red 633),lipoperoxidation(C11 BODIPY 581/591),intracellular reactive oxygen species(ROS)production and DNA status(SCSAR)monitored during 96 *** results show that NE could be used to maintain ram spermatozoa during transport at 15 and 22℃for up to 96 h,with no appreciable loss of kinematic and physiological characteristics of freshly collected *** The storage of ram spermatozoa in liquid form for 2-5 d with vitamin E nanoemulsions may lead more flexibility to breeders in AI *** view of the potential and high versatility of these nanodevices,further studies are being carried out to assess the proposed sperm preservation medium on fertility after artificial insemination.

关键词： Breeding technology Nanoemulsions Nanotechnology Sperm oxidative stress Vitamin E

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Estrium Whey induced hepatitis in a patient with metastatic breast cancer:Case report

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World Journal of Hepatology 2012年第12期4卷 399-401页

作者： Maria jose Velasco julian Molina Department of Internal Medicine and Department of Oncology Luis Vernaza HospitalGuayaquilEcuador and Mayo Clinic College of MedicineRochesterMN 55905United States Department of Oncology Luis Vernaza HospitalGuayaquilEcuador and Mayo Clinic College of MedicineRochesterMN EC090112United States

Estrium Whey is an alternative nutritional support therapy for ***’s enhanced with specific nutrients including phytoestrogens,folate,antioxidants and fiber to support healthy estrogen detoxification and hormone *** describe the first case of hepatotoxicity due to Estrium Whey in a 51-year old female with metastatic breast cancer with clinical,laboratory and histopathological changes.

关键词： Estrium Whey Hepatotoxicity Metastatic disease

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Correlations between corneal and optic nerve head variables in healthy subjects and patients with primary open angle glaucoma

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International Journal of Ophthalmology(English edition) 2015年第6期8卷 1156-1161页

作者： Federico Saenz-Frances Luis Janez Lara Borrego-Sanz Clara Berrozpe-Villabona jose Maria Martinez-de-la-Casa Laura Morales-Fernandez julian Garcia-Sanchez Enrique Santos-Bueso julian Garcia-Feijoo Department of Ophthalmology Clinico San Carlos HospitalComplutense University of Madrid Technology of Knowledge Institute Complutense University of Madrid

AIMTo correlate corneal variables (determined using the Pentacam) with optic nerve head (ONH) variables determined using the Heidelberg retina tomograph (HRT) in healthy subjects and patients diagnosed with primary open angle glaucoma (POAG).METHODSMeasurements were made in 75 healthy eyes and 73 eyes with POAG and correlations examined through Pearson correlation coefficients between the two sets of variables in the two subject groups. The corneal variables determined were corneal volume (CVol), central corneal thickness (CCT), overall corneal thickness (OvCT), the mean thickness of a circular zone centered at the corneal apex of 1 mm radius (zone I) and the mean thickness of several concentric rings, also centered at the apex until the limbus, each of 1 mm width (zones II to VI respectively). The ONH variables were determined using the *** following pairs of variables were correlated in the control group: CCT-disc area (DAr) (-0.48; P<0.0001), Zone I-DAr (-0.503; P<0.0001) and Zone II-DAr (-0.443; P<0.0001); and in the POAG group: CCT-cup-to-disc area ratio (CDRa) (-0.402; P<0.0001), Zone I-CDRa (-0.418; P<0.0001), Zone II-CDRa (-0.405; P=0.006), Zone I-cup shape measure (CSM) (-0.415; P=0.002), Zone II-CSM (-0.405; P=0.001), Zone IV-height variation contour (HVC) (0.378; P=0.002); Zone V-HVC (0.388, P<0.0001).CONCLUSIONSIn the healthy subjects, significant negative correlation was detected between central and paracentral corneal thickness and optic disc area. In contrast, the POAG patients showed significant negative correlation between central and paracentral corneal thickness and the cup-disc ratio and CSM, and positive correlation between peripheral corneal thickness and HVC.

关键词： glaucoma optic nerve head corneal thickness

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Dependence of dynamic contour and Goldmann applanation tonometries on peripheral corneal thickness

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International Journal of Ophthalmology(English edition) 2017年第10期10卷 1521-1527页

作者： Federico Saenz-Frances Claudia Sanz-Pozo Lara Borrego-Sanz Luis Ja?ez Laura MoralesFernandez jose Maria Martinez-de-la-Casa julian Garcia-Sanchez julian Garcia-Feijoo Enrique Santos-Bueso Ophthalmology Department Clinico Universitario San Carlos HospitalComplutense University Institute of Knowledge Technology Complutense University

AIM：To determine the effects of peripheral corneal thickness（PCT）on dynamic contour tonometry（DCT）and Goldmann applanation tonometry（GAT）.METHODS：A cross-sectional *** created a software which calculates the corneal contour（CC）as a function of the radius from the corneal apex to each pixel of the *** software generates a central circumference with a radius of 1 mm and the remainder of the cornea is segmented in 5 rings concentric with corneal apex being its diameter not constant around the corneal circumference as a consequence of the irregular CC but keeping constant the diameter of each ring in each direction of the *** was determined as the mean thickness of the most eccentric *** weighted scatterplot smoothing（LOWESS）regression was used to determine the pattern of the relationship between PCT and both DCT and GAT ***,two multivariable linear regression models were *** each of them,the dependant variable was intraocular pressure（IOP）as determined using GAT and DCT *** both of the models the predictive variable was PCT though LOWESS regression pattern was used to model the relationship between the dependant variables and the predictor *** and sex were also introduced control variables along with their first-degree interactions with *** outcome measures include amount of IOP variation explained through regression models（R2）and regression coefficients（B）.RESULTS：Subjects included 109 eyes of 109 healthy *** regression suggested that a 2nd-degree polynomial would be suitable to model the relationshipbetween both DCT and GAT with *** PCT was introduced in both models as a linear and quadratic *** age nor sex nor interactions were statistically significant in both *** GAT model,R2was 17.14%（F=9.02;P=0.0002）,PCT linear term B was-1.163（95%CI：-1.163,-0.617）.PCT quadratic term B was 0.00081（95%CI：0.00043,0.00118）.For DCT model R2was 14.28%（F=9.29;P=0.0002

关键词： Goldmann applanation tonometry dynamic contour tonometry peripheral corneal thickness corneal thickness

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Simultaneous Biparametric Determination of Total Calcium and Potassium in Biological Fluids by Flow Injection Analysis──Use of Powell＇s Method in the System Optimization

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Chemical Research in Chinese Universities 1994年第1期10卷 5-15页

作者： Araujo, Alberto N. Jun, Shiao Lima, jose L.F.C. Alonso, julian Poch, Manel del Valle, Manel

ＳｉｍｕｌｔａｎｅｏｕｓＢｉｐａｒａｍｅｔｒｉｃＤｅｔｅｒｍｉｎａｔｉｏｎｏｆＴｏｔａｌＣａｌｃｉｕｍａｎｄＰｏｔａｓｓｉｕｍｉｎＢｉｏｌｏｇｉｃａｌＦｌｕｉｄｓｂｙＦｌｏｗＩｎｊｅｃｔｉｏｎＡｎａｌｙｓｉｓ──Ｕｓ... 详细信息

ＳｉｍｕｌｔａｎｅｏｕｓＢｉｐａｒａｍｅｔｒｉｃＤｅｔｅｒｍｉｎａｔｉｏｎｏｆＴｏｔａｌＣａｌｃｉｕｍａｎｄＰｏｔａｓｓｉｕｍｉｎＢｉｏｌｏｇｉｃａｌＦｌｕｉｄｓｂｙＦｌｏｗＩｎｊｅｃｔｉｏｎＡｎａｌｙｓｉｓ──ＵｓｅｏｆＰｏｗｅｌｌ＇ｓＭｅｔｈｏｄ...

关键词： Flow-injection analysis Two-component determination Potassiumand calcium Blood serum Powell's optimization

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Intense pulsed light‑based treatment for the improvement of symptoms in glaucoma patients treated with hypotensive eye drops

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Eye and Vision 2024年第2期10卷 12-21页

作者： jose Maria Martinez‑de‑la‑Casa Carlos Oribio‑Quinto Almudena Milans‑del‑Bosch Pilar Perez‑Garcia Laura Morales‑Fernandez Javier Garcia‑Bella jose Manuel Benitez‑del‑Castillo julian Garcia‑Feijoo David P.Pinero Ophthalmology Unit Department of Ophthalmology and ORLFaculty of MedicineHospital Clinico San-CarlosUniversidad Complutense de MadridInstituto de Investigacion Sanitaria del Hospital Clinico San-Carlos(IdISSC)28040 MadridSpain Department of Immunology Ophthalmology and ORLSchool of MedicineInstituto de Investigaciones Oftalmologicas Ramon CastroviejoUniversidad Complutense de MadridMadridSpain Department of Optics Pharmacology and AnatomyUniversity of AlicanteAlicanteSpain

Background:Ocular surface disease in glaucoma patients is a significant ocular co-morbidity that can affect 40%to 59%of these patients *** current study was aimed at evaluating the potential clinical benefit of an intense pulsed light(IPL)-based treatment in glaucomatous patients with ocular surface disease due to prolonged hypotensive eyedrop *** our knowledge,this is the first series analyzing the therapeutic effect of this treatment option in this type of ***:This non-comparative prospective case series study enrolled a total of 30 glaucoma patients ranging in age from 57 to 94 years old and treated with hypotensive eyedrops for years with dry eye *** patients received four sessions of IPL treatment using the Optima IPL system(Lumenis,Yokneam,Israel)adjusted to the official optimized Lumenis *** in symptomatology,corneal staining,conjunctival hyperemia,non-invasive break-up time(NIBUT),tear osmolarity,tear meniscus height(TMH),meiboscore and meibomian gland expressibility was analyzed after ***:Statistically significant reductions were observed after IPL treatment in the symptomatology scores measured with different questionnaires[ocular surface disease index(OSDI),standard patient evaluation of eye dryness(SPEED)and symptom assessment questionnaire in dry eye(SANDE)]as well as with the visual analogue scale(P<0.001).Mean change in OSDI was−15.0±11.3.A significant reduction was found after treatment in the corneal staining score(P<0.001).A significant reduction was found in tear film meniscus height(P=0.012),as well as in tear film osmolarity(P=0.001).A significant reduction was also found in meibomian gland expressibility(P=0.003),changing the percentage of grade 3 eyes from 44.4%before IPL to 17.2%after ***:IPL therapy combined with meibomian gland expression(MGX)seems to be an effective option to improve symptomatology in glaucomatous patients with ocular surface diseas

关键词： Glaucoma Intense pulsed light Dry eye Meibomian glands

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Tests Management in CALIPSOneo: A MDE Solution

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Journal of Software Engineering and Applications 2014年第6期7卷 506-512页

作者： Alberto Salido julian Alberto Garcia Garcia jose Ponce Javier Jesus Gutierrez Software Engineering and Early Testing Group Universidad de Sevilla Sevilla Spain

Testing in Software Engineering is one of the most important phases although, unfortunately, it cannot be always successfully fulfilled due to time constraints. In most cases, the development phase takes more time than it was estimated, entailing negative effects on the testing phase. The delay increases even more in Research and Development (R + D) projects, where the real time to execute tasks is more difficult to control. Model Driven Engineering (MDE) offers a solution to avoid testing costs without affecting the execution quality of the applied test. This paper presents a practical overview of a Model Driven Testing (MDT)-based methodology and its impact on CALIPSOneo project, which was carried out in liaison with Airbus Defense and Space and, particularly, with the Product Lifecycle Management (PLM) department.

关键词： Model-Driven Testing Model-Driven Engineering Product Lifecycle Management

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The GSTP1 gene variant rs1695 is not associated with an increased risk of multiple sclerosis

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Cellular & Molecular Immunology 2015年第6期12卷 777-779页

作者： jose AG Agundez Elena Garcia-Martin Carmen Martinez julian Benito-Leon julian Benito-Leon Jorge Millan-PascualMaria Diaz-Sanchez Patricia Calleja Diana Pisa Laura Turpin-Fenoll Hortensia Alonso-Navarro Lucia Ayuso-Peralta Dolores Torrecillas Esteban Garcla-Albea jose Francisco Plaza-Nieto Felix Javier Jimenez-Jimenez Department of Pharmacology University of Extremadura CAceres Spain Department of Biochemistry and Molecular Biology University of Extremadura Caceres Spain CiBERNED Centro de InvestigaciOn BiomEdica en Red de Enfermedades Neurodegenerativas Instituto de Salud Carlos III Spain Service of Neurology Hospital Universitario Doce de Octubre Madrid Spain Department of Medicine University Complutense Madrid Spain Section of Neurology Hospital La Mancha-Centro Alcazar de San Juan (Ciudad Real) Spain Centro de Biologfa Molecular Severo Ochoa (CSIC-UAM) Facultad de Ciencias Universidad Autonoma Cantoblanco 28049 Madrid Spain Department of Medicine-Neurology Hospital 'Principe de Asturias' Universidad de Alcala Alcala de Henares (Madrid) Spain Section of Neurology Hospital Universitario del Sureste Arganda del Rey (Madrid) Spain Correspondence: Dr FJ Jimenez-Jimenez C/Marroquina 14 3° B E-28030 Madrid Spain.

We analyzed the allelic and genotypic frequencies of the glutathione-S-transferase P1 （GSTPI） rs1695 single nucleotide polymorphism （SNP） in 290 patients with multiple sclerosis （MS） and in 310 healthy controls. We found no significant association between the rs1695 variant and MS. Among MS patients, there was no relationship between the rs1695 variant and either gender, clinical type of MS or the age of onset of MS.

关键词：多发性硬化多态性单核苷酸发病年龄谷胱甘肽基因性别特性一氧化氮胰岛素

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Multi-tissue transcriptomics of a unique monozygotic discordant twin case of severe progressive osseous heteroplasia

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Genes & Diseases 2024年第3期11卷 22-25页

作者： Alberto Gomez-Carballa Maria jose Curras-Tuala Sara Pischedda Miriam Cebey-Lopez jose Gomez-Rial Irene Rivero-Cale Jacobo Pardo-seco Xabier Bello Sandra Viz-Lasheras Antonio Justicia-Grande julian Montoto-Louzao Alba Camino-Mera Isabel Ferreiros-Vidal Maximo Fraga jose RAntunez Rodolfo Gomez Federico Martinon-Torres Antonio Salas Genetics Vaccines and Infections Research Group(GENVIP)Instituto de Investigacion Sanitaria de SantiagoSantiagode CompostelaGalicia 15706Spain Translational Pediatrics and Infectious Diseases Department of PediatricsHospital Clinico Universitario de Santiago de CompostelaServicio Galego de Saude(SERGAS)Galicia 15706Spain Laboratorio de Inmunologia Servicio de Andlisis ClinicosHospital Clinico Universitario de Santiago de CompostelaServicio Galego de Saude(SERGAS)Galicia 15706Spain Unidade de Xenetica Instituto de Ciencias Forenses(INCIFOR)Facultade de MedicinaUniversidade de Santiago de Compostelaand GenPoB Research GroupInstituto de Investigacion SanitariaHospital Clinico Universitario de Santiago de CompostelaServicio Galego de Saude(SERGAS)Galicia 15706Spain Musculoskeletal Pathology Group Instituto de Investigacion Sanitaria de SantiagoServicio Galego de Saude(SERGAS)Santiago de CompostelaGalicia 15706Spain Department of Pathology Hospital Clinico Universitario de Santiago de Compostela(SERGAS)Galicia 15706Spain Unidad Integrada de BiobancosBiomodelos 3D y Modelos Animales IDIS(lnstituto de Investigacion Sanitaria de Santiagode Compostela)Galicia15706Spain Centro de Investigacion Biomedica en Red de Enfermedades Respiratorias(CIBER-ES) MadridSpain

Progressive osseous heteroplasia(POH)is an ultra-rare autosomal dominant disabling disorder characterized by heterotopic ossification(HO).It is caused by heterozygous inactivating mutations in the GNAS(guanine nucleotide-binding protein alpha-stimulating activity polypeptide)***,the molecular mechanisms underlying HO remain poorly *** a treatment for POH is not yet available,the identification of the mechanisms driving POH in affected tissues using gene expression may be of great help to underestand the molecular basis of POH and develop new therapeutic approaches.

关键词： stimulating driving asi

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