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Acellular Mineralized Exoskeleton Shrimp (MES): A Natural Source of Bioactive Factors for Tissue Regeneration—Preliminary Results

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Journal of Biosciences and Medicines 2022年第3期10卷 34-43页

作者： Raquel Zita Gomes Rodrigo Valdoleiros e Silva Américo Afonso Maria Helena Fernandes Mário Vasconcelos josé cavalheiro Faculty of Dental Medicine University of Porto Porto Portugal Private Practice (Raquel Zita Dental Clinic) Porto Portugal i3S—Institute for Research and Innovation in Health University of Porto Porto Portugal INEB—National Institute of Biomedical Engineering Porto Portugal ICBAS—Institute of Biomedical Sciences Abel Salazar University of Porto Porto Portugal School of Medicine University of Minho Braga Portugal Department of Histology Faculty of Dental Medicine University of Oporto Porto Portugal Department of Pharmacology Faculty of Dental Medicine University of Oporto Porto Portugal Department of Biomaterials Faculty of Dental Medicine University of Oporto Porto Portugal Faculty of Engineering University of Oporto Porto Portugal

Current challenges in the development of scaffolds for bone regeneration include the engineering of biomaterials that can withstand a natural dynamic physiology on the bone that provides a matrix capable of supporting cell migration and tissue ingrowth. The objective of the present work was to develop and characterize a new biomembrane—Mineralized Exoskeleton Shrimp (MES) developed from the exoskeleton of paleomonetes. The integration of MES as a biomaterial for tissue regeneration relies on the growing evidence that the shrimp is characterized by a hierarchically arranged chitin fiber structure, mineralized predominately by calcium carbonate and/or calcium phosphate, bringing beneficial effects in bone regeneration. Additionally, the tridimensional MES structure, can act as a “tent” for Guided Bone Regeneration (GBR). Recently, our team has characterized the MES biomaterial by in vitro (human osteoblastic cellular cultures and immersion of the membrane in modified synthetic plasma) and in vivo (soft tissue in lab mice and hard tissue in rabbit model). The cellular growth in the MES membrane was very exuberant in cellular culture with osteoblastic colonization on its surface (histophilic and biocompatible). After the immersion in modified synthetic plasma for one week, a mass mineralization occurred throughout the membrane’s surface (bioactive). The analysis of histological samples from experimental surgery in lab mice showed that the MES membrane wasn’t toxic to soft tissues and that it caused a moderate inflammatory response (first reabsorption signs at 8 weeks). The MES could act as a cell-guiding template that contains the necessary cues and adequate three-dimensional set to facilitate cell adhesion and promote tissue regeneration upon implantation and subsequent biodegradation.

关键词： Guided Bone Regeneration Biomaterial Biocompatibility Oral Surgery

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Determination of Dihydromyricetin in Cassia ferruginea (Schrad.) DC. by Quantitative 1H NMR (qHNMR)

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Journal of Pharmacy and Pharmacology 2019年第5期7卷 255-264页

作者： Alene Cortes de Queiroz e Silva Lilian Cherubin Correia Lucinéia Vizzotto Marconcini Alberto josé cavalheiro Institute of Chemistry Department of Organic ChemistrySao Paulo State University (UNESP)AraraquaraSPBrazil

Cassia ferruginea (Schrad.) DC.(Fabaceae) is a native plant widely used as an ornamental tree and for the restoration of degraded areas in Brazil. Although no previous ethnopharmacological or chemical information was available, bioprospecting studies on this species found out a high concentration of dihydromyricetin (DHM) in different parts of the plant. DHM, also known as (+)-ampelopsin, is a flavonoid that presents important pharmacological and biological activities, including anticancer, cardioprotective and hepatoprotective effects. The aim of this study was to develop and validate a simple method for the determination of DHM in hydroethanolic extracts of C. ferruginea by quantitative 1H NMR (qHNMR) using the internal standard approach. The parameters as linearity, specificity and selectivity, accuracy, precision (repeatability and intermediate) and robustness showed satisfactory results. Five ethanolic extracts from different stages and organs of C. ferruginea were investigated, the DHM concentration ranges from 52.08 ± 3.95 to 201.83 ± 4.71 mg/g. The proposed method appears to be a suitable tool for fast quality control of herbal extracts, with no need of tedious sample preparation and chromatographic separations increasing the efficiency of natural products analysis. This is the first study reporting the presence and determination of DHM in Cassia ferruginea (Schrad.) DC.

关键词： qHNMR dihydromyricetin Cassia ferruginea

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