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Evaluation of a new qPCR test to identify the organisms causing high total bacterial count in bulk tank milk

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Journal of Integrative Agriculture 2018年第6期17卷 1241-1245页

作者： jorgen Katholm Lene Trier Olesen Anders Petersen Snorri Sigurdsson DNA Diagnostic A/S Risskov 8240Denmark ARLA Foods amba Viby 8260Denmark SEGES Aarhus N 8200Denmark

Milk quality in bulk tank milk （BTM） is measured by flow cytometry technology as total bacterial count （TBC） and somatic cell count （SCC）. To investigate SCC problems, culture or PCR can be used to identify mastitis causing bacteria, e.g., Mastit 4, a commercially available qPCR test. TBC in BTM can be investigated further using culture-based methods such as standard plate count, laboratory pasteurization count, coliform count, and spore counts. To our knowledge, no qPCR addressing the bacteria involved in TBC has been commercially introduced. The aim of this study is to evaluate a recently introduced 3-h qPCR test, TBC 4. The TBC 4 qPCR detects four target groups, Pseudomonas, Streptococci, Enterobacteriacea/ Enterococcus, and Bacillus/Clostridia. These target groups relate to problems on the farm such as cooling, mastitis, environment, and silage. We will continue with new research to compare the TBC 4 qPCR test with traditional culture. For this study, BTM samples from different TBC intervals were selected based on BactoCount results found at routine payment investigation at Eurofins laboratory （Vejen, Denmark）. These samples were analyzed using TBC 4 qPCR assay within 24 h. In total, 346 BTM samples were divided into six different intervals of colony forming units （CFU）. For all four targets in each of the different intervals of CFU, the percent of positive samples, the average Ct-value, the percent of positive samples with Ct〈30 and Ct〈25 were calculated. For Pseudomonas, Streptococci, and Enterobacteriacea/Enterococcus, the number of positive samples with lower Ct-values （high bacteria content） correlated with the CFU mL-1. We found Enterobacteriacea/ Enterococcus, Pseudomonas, and Streptococci in high number of bacteria （Ct〈25） in 25, 19 and 56％ of samples with CFU mL-1 between 50001-100000 and 53, 44, and 39％ in samples with CFU mL-1〉100000. The TBC 4 qPCR test showed to be a strong and fast tool for farmers, advisors and service technicians to address probl

关键词： TBC bulk tank milk qPCR milk quality

Wave Analysis of Porous Geometry with Linear Resistance Law

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Journal of Marine Science and Application 2017年第4期16卷 480-489页

作者： jorgen Dokken John Grue Lars Petter Karstensen Mechanics Division Department of Mathematics University of Oslo Oslo 0316 Norway

The wave diffraction-radiation problem of a porous geometry of arbitrary shape located in the free surface of a fluid is formulated by a set of integral equations, assuming a linear resistance law at the geometry. The linear forces, the energy relation and the mean horizontal drift force are evaluated for non-porous and porous geometries. A geometry of large porosity has an almost vanishing added mass. The exciting forces are a factor of 5–20 smaller compared to a solid geometry. In the long wave regime, the porous geometry significantly enhances both the damping and the mean drift force, where the latter grows linearly with the wavenumber. The calculated mean drift force on a porous hemisphere and a vertical truncated cylinder, relevant to the construction of fish cages, is compared to available published results.

关键词： wave analysis fish cages mean drift force wave exciting force added mass damping

Form-Finding of Tensegrity Structures based on the Force Density Method

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Form-Finding of Tensegrity Structures based on the Force Den...

作者： Kitsakou Krimbi jorgen Zitrone 绍兴文理学院

学位级别：硕士

张拉整体结构是一种由连续拉索与离散压杆组合而成的空间索杆张力结构。该结构具有造型独特,质量轻盈、可展等特点,吸引了大量学者的关注并对其力学行为开展了深入研究,于是在众多工程领域中得到了广泛的应用。本文在前人研究基础上,运... 详细信息

张拉整体结构是一种由连续拉索与离散压杆组合而成的空间索杆张力结构。该结构具有造型独特,质量轻盈、可展等特点,吸引了大量学者的关注并对其力学行为开展了深入研究,于是在众多工程领域中得到了广泛的应用。本文在前人研究基础上,运用力密度法寻找几何稳定的新型张拉整体结构,使得该结构具有形式灵活的特点且可以保持初始几何稳定。利用MATLAB软件,基于平衡矩阵理论和矩阵奇异值分解理论,求解结构初始状态时的力密度。本文在理论部分详细介绍了力密度法、矩阵分析理论及几何稳定判定标准,建立了力密度矩阵谱分解与平衡矩阵特征值分解的循环迭代关系,有效求解了单自应力模态或多自应力模态张拉整体结构的初始力密度。同时提出了四种不同类型的新型张拉整体结构,并对其进行了基于MATLAB软件的计算分析。研究结果表明,本文所提出的四种张拉整体结构均满足结构几何稳定要求,验证了本文算法的可行性和准确性。本文总结了张拉整体结构找形理论基础,完善了该类结构体系形态发展的框架体系。提出的算法可切实有效的解决新型张拉整体结构的初始形态确定问题。此外,提出的四种新型张拉整体结构模型丰富了现有的结构形态,为后续设计和寻找更为复杂的、非对称的张拉整体结构提供了一定的理论基础。

关键词：张拉整体结构模型找形奇异值分解平衡矩阵

Serum-induced degradation of 3D DNA box origami observed with high-speed atomic force microscopy

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Nano Research 2015年第7期8卷 2170-2178页

作者： Zaixing Jiang Shuai Zhang Chuanxu Yang jorgen Kjems Yudong Huang Flemming Besenbacher Mingdong Dong Department of Polymer Science and Technology School of Chemical Engineering and Technology Harbin Institute of Technology Harbin 150001 China Interdisciplinary Nanoscience Center (iNANO) Aarhus University DK-8000 Aarhus C Denmark These authors contributed equally to this study.

3D DNA origami holds tremendous potential for the encapsulation and selective release of therapeutic drugs. Observations of the real-time performance of these structures in physiological environments will contribute to the development of future applications. We investigated the degradation kinetics of 3D DNA box origami in serum by using high-speed atomic force microscope optimized for imaging 3D DNA origami in real time. The time resolution allowed to characterize the stages of serum effects on individual 3D DNA boxes origami with nanometer resolution. Our results indicate that the digestion process is a combination of rapid collapse and slow degradation phases. Damage to box origami occurs mainly in the collapse phase. Thus, the structural stability of 3D DNA box origami should be improved, especially in the collapse phase, before these structures are used in clinical applications.

关键词： 3D DNAbox origami,high-speed AFM.stability,serum,kinefics

The Mediator. Richard III and Macbeth in Einar MurGudmundsson＇ s Novel Angels of the Universe

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Journal of Literature and Art Studies 2018年第2期8卷 216-221页

作者： jorgen SteenVeisland University of Gdansk Gdansk Poland

Gudmundsson＇s Angels of the Universe （Englaralheimsins, 1993） stages a poetic psychodrama weaving Shakespeare＇s characters Richard IlI and Macbeth into the lives of the first-person narrator Paul and his friend Viktor who are undergoing intensive drug treatment at the Klepp hospital in Reykjavik. Viktor starts impersonating Richard III prior to the treatment at Klepp. While enrolled at an English university he assumes the physical and mental guise of Richard, memorizing whole scenes from the play and speaking lines out loud. At some point his perfect English shifts to perfect German as he merges Richard III with Adolf Hitler in his mind. Viktor thus turns the two despots into what Rene Girard in his work Deceit, Desire and the Novel calls the mediator. Girard explores ＂triangular＂ desire in Cervantes＇ knight-errant Don Quixote whose mediator Amadis chooses the objects of the knight＇s desire. With Dostoyevsky external mediation becomes internal mediation whose main features are impotence and alienation. Viktor exhibits these symptoms as a negative, inverted form of the mediator has usurped his personality while relegating the objects of his desire to the background. There are no objects any more. He is alone. The article aims to disclose the complex cultural and sociopsychological reasons for exclusi6n and to explore the poetic dimensi6n of the novel, indicating thst poetry is capable of transcending the limits imposed by society.

关键词： impersonation psychiatric treatment exclusion loneliness poetry

Development of a computer vision system to monitor pig locomotion

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Open Journal of Animal Sciences 2013年第3期3卷 254-260页

作者： jorgen Kongsro Norsvin HamarNorway

Avoiding lameness or leg weakness in pig production is crucial to reduce cost, improve animal welfare and meat quality. Detection of lameness detection by the use of vision systems may assist the farmer or breeder to obtain a more accurate and robust measurement of lameness. The paper presents a low-cost vision system for measuring the locomotion of moving pigs based on motion detection, frame-grabbing and multivariate image analysis. The first step is to set up a video system based on web camera technology and choose a test area. Secondly, a motion detection and data storage system are used to build a processing system of video data. The video data are analyzed measuring the properties of each image, stacking them for each animal and then analyze these stacks using multivariate image analysis. The system was able to obtain and decompose information from these stacks, where components could be extracted, representing a particular motion pattern. These components could be used to classify or score animals according to this pattern, which might be an indicator of lameness. However, further improvement is needed with respect to standardization of herding, test area and tracking of animals in order to have a robust system to be used in a farm environment.

关键词： Video image Analysis Multivariate Image Analysis Principal Component Analysis Leg Weakness Structural Soundness Pigs

Clinical neurological examination vs electrophysiological studies:Reflections from experiences in occupational medicine

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World Journal of Methodology 2015年第2期5卷 26-30页

作者： jorgen Riis Jepsen Department of Occupational Medicine Hospital of South-western Jutland

Seventy-five percent of upper limb disorders that are related to work are regarded as diagnostically unclassifiable and therefore challenging to the *** it has been generally less successfully toprevent and treat these common and frequently disabling *** reach a diagnosis requires the identification of the responsible pathology and the involved tissues and ***,improved diagnostic approaches are *** editorial discusses the potentials of using the clinical neurologic examination in patients with upper limb complaints related to *** is argued that a simple but systematic physical approach permits the examiner to frequently identify patterns of neurological findings that suggest nerve afflictions and their locations,and that electrophysiological studies are less likely to identify pathology.A diagnostic algorithm for the physical assessment is provided to assist the *** to include representative neurological items in the physical examination may result in patients being misinterpreted,misdiagnosed and mistreated.

关键词： Neurological examination Electrophysiology Work-related disorders Occupational medicine Nerve affictions Upper limb disorders

Does the Environmental Gain of Switching to the Healthy New Nordic Diet Outweigh the Increased Consumer Cost？

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Journal of Food Science and Engineering 2014年第6期4卷 291-300页

作者： Henrik Saxe jorgen Dejgard Jensen DTU Management Engineering Global Decision Support Initiative Produktionstorvet B424 DK-2800 Kgs. Lyngby Denmark Department of Food and Resource Economics University of Copenhagen Rolighedsvej 25 DK-1958 Frederiksberg C Denmark

The new Nordic diet （NND） was designed by gastronomic, nutritional and environmental specialists to be a palatable, healthy and sustainable diet containing 30%-40% less meat than the average Danish diet （ADD）, ≥ 75% organics, and more locally grown wholegrain products, nuts, fruit and vegetables. In this study, the NND was based on economic modelling to represent a ＂realistic NND bought by Danish consumers＂. The objective was to investigate whether the ADD-to-NND diet-shift has environmental consequences that outweigh the increased consumer cost of the diet-shift. The diet-shift reduced the three most important environmental impacts by 16%-22%, mainly caused by reduced meat content. The surcharge to consumers of the ADD-to-NND diet-shift was ∈216/capita/year. In monetary terms, the savings related to the environmental impact of the diet-shift were ∈151/capita/year. 70% of the increased consumer cost of the ADD-to-NND diet-shift was countered by the reduced socioeconomic advantage associated with the reduced environmental impact of the NND.

关键词： Environmental impact health life cycle assessment meat new Nordic diet OPUS organics socioeconomic cost.

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Contributed papers:Fungi 2