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Pe.coll Gradie.t Optimization for Blood CD133+ Ste. Ce.l Re.ove.y

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Ste. Ce.l Discove.y 2014年第3期4卷 61-66页

作者： Rosa María Lópe.-Franco jorge e. moreno-cuevas María Te.e.a Gonzále.-Garza Se.vicio de.Te.apia Ce.ular School of Medicine CITES Tecnológico de Monterrey Monterrey México

Circulating CD133+ ste. ce.ls from the.pe.iphe.al blood have.be.n shown to be.able.to diffe.e.tiate.into nume.ous ce.l line.ge.. Howe.e., adults have.only a small numbe. of the.e.circulating ste. ce.ls. The.aim of the.pre.e.t study was to asse.s a ne. isolation and e.richme.t te.hnique.for CD133+ ste. ce.ls from pe.iphe.al blood with the.use.of Pe.coll de.sity gradie.ts. Our re.ults de.onstrate. the.pre.e.ce.of two large.mononucle.r bands whe. whole.blood was ce.trifuge. with 48% and 50% Pe.coll conce.trations. Flow cytome.ric analysis (FACS) re.e.le. a major CD133+ e.richme.t at the.48% Pe.coll conce.tration in one.of the.two bands. Furthe. culture.of the.e.ce.ls re.ulte. in the.formation of multiple.colony-forming units. Our re.ults sugge.t an advantage.from using a simple.Pe.coll gradie.t for succe.sful CD133+ ce.l re.ove.y, which could aid in diffe.e.tiation and transplantation protocols.

关键词： Ste. Ce.ls Re.ove.y CD133+ Pe.coll De.sity Gradie.ts

e.graftme.t of pre.diffe.e.tiate. ste. ce.ls into cardiomyocyte. in an animal mode. of ische.ic cardiopathy

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Ste. Ce.l Discove.y 2014年第1期4卷 1-7页

作者： Maria Te.e.a Gonzale.-Garza Citlali Alcaraz Lizbe.h Gonzale.-Jara De.e.rio Arcos jorge e. moreno cuevas Se.vicio de.Te.apia Ce.ular School of Medicine Centro de Innovación y Transferencia en Salud Tecnológico de Monterrey Monterrey México

Ste. ce.l the.apy for cardiac infarct re.e.e.ation has be.n wide.y use. in clinical re.e.rch. De.pite.the.fact that important advance. in this fie.d have.be.n re.che., the.obse.ve. re.ove.y doe. not de.onstrate.ne. cardiac muscle.formation. Be.e.its have.be.n obse.ve. due.to an improve.e.t in ne.vascularisation. The.main obje.tive.of this study was to de.e.mine.if pre.diffe.e.tiate. ste. ce.ls into ce.ls of myocardic line.ge.are.capable.of e.graftme.t in animal mode.s with induce. cardiac infarct and are.capable.of truly diffe.e.tiating into myocardiocyte.. Bone.marrow rat ste. ce.ls we.e.pre.diffe.e.tiate. with 5-AZ. Afte. 4 we.ks, pre.diffe.e.tiate. ste. ce.ls e.pre.s muscarinic 1, 2 and β adre.e.gic 2 re.e.tors. Also, prote.ns such as sarcome.ic α-actin, cardiac myosin he.vy chain, de.min and vime.tin we.e.de.e.te. by immunocytoche.istry. Ce.ls we.e.transplante. intracardialy in an ische.ic cardiac rat mode.. Pre.diffe.e.tiate. or non diffe.e.tiate. ce.ls we.e.transplante. afte. 4 we.ks post infarct induction. Histopathology of the.he.rts was made.2 we.ks afte. ce.l transplantation. Typical granulate. tissue. scare.formation and ne.vascularisation we.e.obse.ve. in both groups. Howe.e., in those.he.rts from rats inoculate. with pre.diffe.e.tiate. ce.ls many appe.re. atypical and we.e.α-actin sarcome.ic positive. The.e.e.e.ts sugge.t that pre.diffe.e.tiate. ce.ls conse.ve.some.muscle.characte.istic traits in situ that at le.st last for two we.ks afte. transplantation.

关键词： Ce.l The.apy MSC Re.e.e.ative Me.icine Cardiocyte Diffe.e.tiation Ste. Ce.ls

Dire.t pre.diffe.e.tiation of rat me.e.chymal ste. ce.ls into dopamine.gic ce.ls

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Ste. Ce.l Discove.y 2013年第2期3卷 133-138页

作者： Judith Zavala-Arcos Maria Te.e.a Gonzale.-Garza Jane. Gutie.re.-Alcala He.tor R. Martine. jorge e. moreno-cuevas Se.vicio de.Te.apia Ce.ular School of Medine and Health CITES Tecnológico de Monterrey Monterrey México

The.use.of ste. ce.ls has be.n propose. as an alte.native.tre.tme.t for ce.tain ne.rode.e.e.ative.disorde.s. It has also be.n sugge.te. that in the.pre.diffe.e.tiate. state. ste. ce.ls might provide.a be.te. the.ape.tic option than ce.ls that are.undiffe.e.tiate. or fully diffe.e.tiate.. The.purpose.of this study was to de.e.op a protocol aime. at re.ucing the.incubation time.re.uire. to induce.the.conve.sion of rat me.e.chymal ste. ce.ls into immature.dopamine.gic ne.rons. Ste. ce.ls obtaine. from rat bone.marrow we.e.incubate. in a control or induction me.ia for 2-24 h. Ce.ls incubate. for 24 h in induction me.ium de.onstrate. an incre.se.on the.le.e.s of the.ne.ronal prote.n marke.s ne.tin, glial fibrillary acid prote.n, and β-tubulin III, as we.l as incre.se. in the.e.pre.sion of Pax3, e.1, Thy1.1, and Ge.10 ge.e.. This manuscript pre.e.ts e.ide.ce.that adult me.e.chymal ce.ls are.capable.to re.pond, in a short time.pe.iod, to a ne.roinduction me.ium, and give.raise.to pre.diffe.e.tiate. ne.ron like.ce.ls re.re.e.ting an alte.native.for Parkinson dise.se.ce.l the.apy transplantation.

关键词： Ce.l Diffe.e.tiation Dopamine Producing Ce.ls Ne.ro-Diffe.e.tiation Parkinson’s Dise.se Ste. Ce.ls

Comparative.e.aluation of a Modifie. Ace.ic Me.hod for e.traction of Antioxidant Compounds from Black Be.ns (<i>Phase.lus vulgaris</i>)

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Food and Nutrition Scie.ce. 2012年第3期3卷 348-353页

作者： Jose.F. Islas Alfre.o J. Dávalos-Balde.as Ne.zahualcóyotl Arroyo-Currás Be.man G. Cano Paulina Galindo-Jacobo Gustavo Guajardo-Salinas Ange. Gaytan-Ramos jorge e. moreno-cuevas Laboratorio de.Te.apia Ce.ular Centro de Investigación y Transferencia en Salud (CITES)-Escuela de Medicina Ignacio A. Santos Monterrey México.

e.traction of phe.olic compounds from be.n se.d coats by ace.ic acid was e.aluate. and compare. to conve.tional e.traction with 80% me.hanol. Total phe.ols, flavonoids and fre. radical scave.ging capacity we.e.de.e.mine. by colorime.ric me.hods. Furthe.more. qualitative.flavonoid characte.ization was pe.forme. via HPLC diode.array. The.ace.ic acid e.tract containe. 73.4 ± 7 mg/g of sample.e.pre.se. as gallic acid e.uivale.ts (GAe., and 41.3 ± 4 mg/g as cate.hin e.uivale.ts (Ce.. The.ace.ic acid e.tract (at 1 mg/mL) showe. ove. 89% e.ficie.cy to scave.ge.the.1,1 diphe.yl-2-picrylhydrazil (DPPH) radical (e.50 = 6.13 mg e.tract/mg standard). Gallic acid, de.phinidin-3-glycoside. pe.unidinglycoside. pe.unidin, malvidin-3-glycoside. myrice.in-glycoside. que.citin-glycoside. saponin (β-g), and 3-hydroxy-5, 7-me.astigmadie.-9-one.we.e.ide.tifie. in both e.tracts. Additionally, kae.pfe.ol-3-glycoside.was found in the.ace.ic acid e.tract. Se.ue.tial ace.ic acid e.tractions indicate. that the.antiradical activity was about 90% in DPPH radical scave.ging within the.first 8 h of e.traction, whe. compare. to a 24 h e.traction. In ge.e.al, ace.ic acid offe.e. a faste. and more.e.ficie.t approach for the.e.traction of

关键词： Ace.ic Acid Antioxidants DPPH Scave.ging e.traction Black Be.ns

Brain scarring in infants:immunological insights from a ne.natal hypoxic-ische.ic e.ce.halopathy mode.

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Ne.ral Re.e.e.ation Re.e.rch 2025年第10期20卷 2909-2910页

作者： Pe.ro moreno Pime.te.-Coe.ho Carlos Chagas Filho Biophysics Institute Federal University of Rio de JaneiroRio de JaneiroBrazil

Ne.natal hypoxic-ische.ic e.ce.halopathy(HIe.is a significant cause.of disability in *** brain function and acce.e.ating ne.rological re.ove.y may re.uire.a combination of ne.roprote.tive.and pro-re.e.e.ative.tre.tme.ts at diffe.e.t stage. of *** the.first hours afte. the.ne.natal insult are.the.most critical pe.iod for ne.roprote.tion,the.e.iste.ce.of se.ondary and te.tiary me.hanisms of brain injury offe.s the.possibility of pre.e.ting de.aye. ne.rode.e.e.ation in the.subse.ue.t days,we.ks,or months(Le.ison e. al.,2022).

关键词： ne.natal prote.tive hypoxic

The.e.e.ging role.of me.e.chymal ste. ce.l-de.ive. e.trace.lular ve.icle. to ame.iorate.hippocampal NLRP3 inflammation induce. by binge.like.e.hanol tre.tme.t in adole.ce.ce

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Ne.ral Re.e.e.ation Re.e.rch 2025年第4期20卷 1153-1163页

作者： Susana Me.lado María JoséMorillo-Bargue. Carla Pe.piñá-Clérigue. Francisco García-García Victoria moreno-Manzano Consue.o Gue.ri María Pascual De.artme.t of Physiology School of Medicine and DentistryUniversity of ValenciaValenciaSpain Bioinformatics and Biostatistics Unit Príncipe Felipe Research CenterValenciaSpain Ne.ronal and Tissue.Re.e.e.ation Laboratory Príncipe Felipe Research CenterValenciaSpain

Our pre.ious studie. have.re.orte. that activation of the.NLRP3(NOD-,LRR-and pyrin domain-containing prote.n 3)-inflammasome.comple. in e.hanol-tre.te. astrocyte. and chronic alcohol-fe. mice.could be.associate. with ne.roinflammation and brain *** ste. ce.l-de.ive. e.trace.lular ve.icle.(MSC-e.s)have.be.n shown to re.tore.the.ne.roinflammatory re.ponse.along with mye.in and synaptic structural alte.ations in the.pre.rontal corte.,and alle.iate.cognitive.and me.ory dysfunctions induce. by binge.like.e.hanol tre.tme.t in adole.ce.t *** the.the.ape.tic role.of the.mole.ule. containe. in me.e.chymal ste. ce.l-de.ive. e.trace.lular ve.icle.,the.pre.e.t study analyze. whe.he. the.administration of me.e.chymal ste. ce.l-de.ive. e.trace.lular ve.icle. isolate. from adipose.tissue.which inhibite. the.activation of the.NLRP3 inflammasome.was capable.of re.ucing hippocampal ne.roinflammation in adole.ce.t mice.tre.te. with binge.*** de.onstrate. that the.administration of me.e.chymal ste. ce.l-de.ive. e.trace.lular ve.icle. ame.iorate. the.activation of the.hippocampal NLRP3 inflammasome.comple. and othe. NLRs inflammasome.(e.g.,pyrin domain-containing 1,caspase.re.ruitme.t domain-containing 4,and abse.t in me.anoma 2,as we.l as the.alte.ations in inflammatory ge.e.(inte.le.kin-1β,inte.le.kin-18,inducible.nitric oxide.synthase.nucle.r factor-kappa B,monocyte.che.oattractant prote.n-1,and C–X3–C motif che.okine.ligand 1)and miRNAs(miR-21a-5p,miR-146a-5p,and miR-141-5p)induce. by binge.like.e.hanol tre.tme.t in adole.ce.t *** analysis furthe. re.e.le. the.involve.e.t of miR-21a-5p and miR-146a-5p with inflammatory targe. ge.e. and NOD-like.re.e.tor signaling *** toge.he.,the.e.findings provide.nove. e.ide.ce.of the.the.ape.tic pote.tial of MSC-de.ive. e.s to ame.iorate.the.hippocampal ne.roinflammatory re.ponse.associate. with NLRP3 inflammasome.activation induce. by binge.drinking in adole.ce.ce.

关键词： adole.ce.ce binge.like.e.hanol tre.tme.t e.trace.lular ve.icle. hippocampus me.e.chymal ste. ce.ls ne.roinflammation NOD-,LRR-and pyrin domain-containing prote.n 3(NLRP3)

The.se.ually dimorphic e.pre.sion of glutamate.transporte.s and the.r implication in pain afte. spinal cord injury

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Ne.ral Re.e.e.ation Re.e.rch 2025年第11期20卷 3317-3329页

作者： Je.nife. M.Colón-Me.cado Aranza I.Torrado-Tapias Iris K.Salgado Jose.M.Santiago Samue. e.Ocasio Rive.a Dina P.Bracho-Rincon Luis H.Pagan Rive.a jorge D.Miranda De.artme.t of Physiology University of Puerto RicoMedical Sciences CampusSan JuanPRUSA National Institute. of He.lth National Human Genome Research InstituteBethesdaMDUSA Unive.sidad Ce.tral de. Caribe School of MedicineBayamónPRUSA Unive.sity of Pue.to Rico Carolina CampusCarolinaPRUSA Institute.of Ne.robiology San JuanPRUSA

In addition to the.loss of motor function,~60% of patie.ts de.e.op pain afte. spinal cord *** ce.lular-mole.ular me.hanisms are.not we.l unde.stood,but the.data sugge.ts that plasticity within the.rostral,e.ice.te.,and caudal pe.umbra of the.injury site.initiate. a ce.lularmole.ular inte.play that acts as a re.iring me.hanism le.ding to ce.tral ne.ropathic *** can le.d to the.formation of ne. conne.tions trigge.ing abnormal se.sory *** e.citatory glutamate.transporte.s are.re.ponsible.for the.re.ptake.of e.trace.lular glutamate.which make. the. a critical targe. to pre.e.t ne.ronal hype.e.citability and *** pre.ious studie. showe. a se.ually dimorphic the.ape.tic window for spinal cord injury afte. tre.tme.t with the.se.e.tive.e.troge. re.e.tor modulator *** this study,we.inve.tigate. the.anti-allodynic e.fe.ts of tamoxife. in male.and fe.ale.rats with spinal cord *** hypothe.ize. that tamoxife. e.e.ts anti-allodynic e.fe.ts by incre.sing the.e.pre.sion of glutamate.transporte.s,le.ding to re.uce. hype.e.citability of the.se.ondary ne.ron or by de.re.sing abe.rant *** and fe.ale.rats re.e.ve. a mode.ate.contusion to the.thoracic spinal cord followe. by subcutane.us slow-re.e.se.tre.tme.t of tamoxife. or matrix pe.le.s as a control(place.o).We.use. von Fre. monofilame.ts and the.up-down me.hod”to e.aluate.me.hanical *** tre.tme.t de.re.se. allodynia only in fe.ale.rats with spinal cord injury re.e.ling a se.de.e.de.t *** e.pre.sion profile.of glutamate.gic transporte.s(e.citatory amino acid transporte. 1/glutamate.aspartate.transporte. and e.citatory amino acid transporte. 2/glutamate.transporte.-1)re.e.le. a se.ual dimorphism in the.rostral,e.ice.te.,and caudal are.s of the.spinal cord with a patte.n of e.pre.sion primarily on *** rode.ts showe. a significantly highe. le.e. of e.citatory amino acid transporte.-1 e.pre.sion while.male.rode.ts showe. incre.se.

关键词： allodynia ce.tral ne.ropathic pain e.AT-1/GLAST e.AT-2/GLT-1 glutamate.transporte.s se.e.tive.e.troge. re.e.tor modulator se.ual dimorphism spinal cord injury trauma

Diffe.e.tial re.ponse.of injure. and he.lthy re.inas to synge.e.c and alloge.e.c transplantation of a clonal ce.l line.of immortalize. olfactory e.she.thing glia:a double.e.ge. sword

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Ne.ral Re.e.e.ation Re.e.rch 2025年第8期20卷 2395-2407页

作者： María Norte.Muñoz María Porte.a-Lomba Paloma Sobrado-Calvo Diana Simón Johnny Di Pie.dome.ico Ale.andro Galle.o-Orte.a Mar Pére. JoséMCabre.a-Maque.a Javie. Sie.ra Manue. Vidal-Sanz María Te.e.a moreno-Flore. Marta Agudo-Barriuso Grupo de.Inve.tigación Oftalmología e.pe.ime.tal Departamento de OftalmologíaOptometríaOtorrinolaringología y Anatomía PatológicaFacultad de MedicinaUniversidad de MurciaInstituto Murciano de Investigación Biosanitaria(IMIB)Campus de Ciencias de la SaludMurciaSpain e.pe.ime.tal Scie.ce. Faculty Universidad Francisco de VitoriaPozuelo de AlarcónMadridSpain Anatomy Histology and Neuroscience DepartmentMedicine FacultyUniversidad Autónoma de MadridMadridSpain Ce.te. of Ne.roimmunology Service of NeurologyLaboratory of Advanced Imaging in Neuroimmunological DiseasesHospital Clínic of BarcelonaInstitut d’Investigacions Biomèdiques August Pi i Sunyer(IDIBAPS)and Universitat de BarcelonaBarcelonaSpain Me.icine.Faculty Universidad Francisco de VitoriaPozuelo de AlarcónMadridSpain

Olfactory e.she.thing glia promote.axonal re.e.e.ation in the.mammalian ce.tral ne.vous syste.,including re.inal ganglion ce.l axonal growth through the.injure. optic ***,it is unknown whe.he. olfactory e.she.thing glia also have.ne.roprote.tive.*** e.she.thing glia e.pre.s brain-de.ive. ne.rotrophic factor,one.of the.be.t ne.roprote.tants for axotomize. re.inal ganglion ***,we.aime. to inve.tigate.the.ne.roprote.tive.capacity of olfactory e.she.ting glia afte. optic ne.ve.*** e.she.thing glia ce.ls from an e.tablishe. rat immortalize. clonal ce.l line.Te.3,we.e.intravitre.lly inje.te. in intact and axotomize. re.inas in synge.e.c and alloge.e.c mode.with or without microglial inhibition or immunosuppre.sive.*** and ge.e.e.pre.sion analyse. we.e.*** bulb-de.ive. primary olfactory e.she.thing glia and Te.3 e.pre.s major histocompatibility comple. classⅡ*** and synge.ically transplante. Te.3 ce.ls survive. in the.vitre.us for up to 21 days,forming an *** axotomize. re.inas,only the.alloge.e.c Te.3 transplant re.cue. re.inal ganglion ce.ls at 7 days but not at 21 *** the.e.re.inas,microglial anatomical activation was highe. than afte. optic ne.ve.crush *** intact re.inas,both transplants activate. microglial ce.ls and cause. re.inal ganglion ce.l de.th at 21 days,a loss that was highe. afte. allotransplantation,trigge.e. by pyroptosis and partially re.cue. by microglial inhibition or ***,ne.roprote.tion of axotomize. re.inal ganglion ce.ls did not improve.with the.e.*** diffe.e.t ne.roprote.tive.prope.tie.,diffe.e.t toxic e.fe.ts,and diffe.e.t re.ponse. to microglial inhibitory tre.tme.ts of olfactory e.she.thing glia in the.re.ina de.e.ding on the.type.of transplant highlight the.importance.of thorough pre.linical studie. to e.plore.the.e.variable..

关键词： ce.l the.apy immune.re.ognition major histocompatibility comple. class II(MHCII) ne.roprote.tion olfactory e.she.thing glia re.inal ganglion ce.ls

Structure.stability,catalytic activity,and polarizabilitie. of small iridium cluste.s

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Chine.e.Physics B 2018年第6期27卷 225-230页

作者： Francisco e.jorge José R da Costa Ve.aancio Unidade.Acade.ica dc Ffsica Universidade Federal de Campina Grande 58429-900 Campina Grande Brazil De.artame.to de.Ffsica Universidade Federal do Espfrito Santo 29060-900 Vitoria Brazil

At the.se.ond orde. Douglas–Kroll–He.s（DKH2） le.e., the.B3 PW91 functional in conjunction with the.re.ativistic all-e.e.tron basis se. of vale.ce.triple.ze.a quality plus polarization functions are.e.ploye. to compute.bond le.gths, dissociation e.e.gie., ve.tical ionization pote.tials, and the.highe.t occupie. mole.ular orbital-lowe.t unoccupie. mole.ular orbital e.e.gy gaps of the.small iridium cluste.s（Irn, n≤8）. The.e.re.ults are.compare. with the.e.pe.ime.tal and the.re.ical data available.in the.lite.ature. Our re.ults confirm the.the.re.ical pre.ictions made.by Fe.g e. al. about the.catalytic activitie. of the.Ir4 and Ir6 cluste.s. From the.optimize. ge.me.rie., DKH2 calculations of static e.e.tric me.n dipole.polarizabilitie. and polarizability anisotropie. are.also carrie. out. It is the.first time.that the.polarizabilitie. of small iridium cluste.s have.be.n studie.. For n≤4, the.me.n dipole.polarizabilitie. pe. atom pre.e.t an odd–e.e. oscillatory be.avior,whe.e.s from Ir5 to Ir8, the. de.re.se.with the.cluste. size.incre.sing. The.de.e.de.ce.of the.polarizability anisotropy on the.structure.symme.ry of the.iridium cluste. is ve.ifie..

关键词： B3PW91 functional all-e.e.tron basis se.s DKH2 calculations iridium cluste.s polarizabilitie.