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Improving the haploidization toolbox:maternal factors take the stage

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molecular Plant 2023年第4期16卷 651-653页

作者： maria Flores-Tornero Helena Sapeta jorg D.Becker Instituto de Tecnologia Quimica e Biologica Universidade Nova de LisboaAv.daRepublica2780-157OeirasPortugal

Plant breeders have a great interest in obtaining plants that are genetically homogeneous as quickly as possible,with high efficiency and at low *** approaches,including selfing or backcrossing,cannot provide *** technologies,however,offer a solution and are therefore the subject of intensive research,in particular during the last decade.

关键词： breed homogeneous quickly

Colorful Insights： Advances in Imaging Drive Nove 3reakthroughs in Ca^2＋ Signaling

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molecular Plant 2015年第3期8卷 352-355页

作者： Alex Costa jorg Kudla Dipartimento di Bioacienze Università degli Studi di Milano Via G. Celoria 26 20133 Milan Italy Istituto di Biofisica Consiglio Nazionale delle Ricerche Via G. Celoria 26 20133 Milan Italy Institut für Biologie und Biotechnologie der Pflanzen Universitat Münster Schlossplatz 4 48149 Münster Germany

In plants, Ca^2＋ signals occur in response to both environmental and developmental stimuli. These signals involve increases in [Ca^2＋] in compartments such as the cytoplasm, nucleus, and mitochondria, and can display multiple shapes ranging from sin- gle transients to repetitive Ca^2＋ oscillations. Generation and shaping of stimulus specific [Ca^2＋] signatures depends on Ca^2＋ influxes and effluxes occurring at both the plasma membrane （Pm） and membranes of the different subcellular compartments.

关键词： @@

Effects of Resistant Starch on Symptoms,Fecal markers,and Gut microbiota in Parkinson’s Disease—The RESISTA-PD Trial

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Genomics, Proteomics & Bioinformatics 2022年第2期20卷 274-287页

作者： Anouck Becker Georges Pierre Schmartz Laura Groger Nadja Grammes Valentina Galata Hannah Philippeit jacqueline Weiland Nicole Ludwig Eckart meese Sascha Tierling jorn Walter Andreas Schwiertz jorg Spiegel Gudrun Wagenpfeil Klaus Faßbender Andreas Keller marcus m.Unger Department of Neurology Saarland UniversityD-66421 HomburgGermany Chair for Clinical Bioinformatics Saarland UniversityD-66123 SaarbruckenGermany Department of Human Genetics Saarland UniversityD-66421 HomburgGermany Department of Genetics/Epigenetics Saarland UniversityD-66123 SaarbruckenGermany Institute of microecology D-35745 HerbornGermany Institute of medical Biometry Epidemiology and Medical InformaticsSaarland UniversityD-66421 HomburgGermany Department of Neurology Stanford UniversityPalo AltoCA 94305USA

The composition of the gut microbiota is linked to multiple diseases,including Parkinson’s disease(PD).Abundance of bacteria producing short-chain fatty acids(SCFAs)and fecal SCFA concentrations are reduced in *** exert various beneficial functions in *** the interventional,monocentric,open-label clinical trial “Effects of Resistant Starch on Bowel Habits,Short Chain Fatty Acids and Gut microbiota in Parkinson’s Disease”(RESISTA-PD;ID:NCT02784145),we aimed at altering fecal SCFAs by an 8-week prebiotic intervention with resistant starch(RS).We enrolled 87 subjects in three study-arms:32 PD patients received RS(PD+RS),30 control subjects received RS,and 25 PD patients received solely dietary *** performed paired-end 100 bp length metagenomic sequencing of fecal samples using the BGISEQ platform at an average of 9.9 *** was *** the PD+RS group,fecal butyrate concentrations increased significantly,and fecal calprotectin concentrations dropped significantly after 8 weeks of RS ***,we observed a reduction in non-motor symptom load in the PD+RS *** reference-based analysis of metagenomes highlighted stable alpha-diversity and beta-diversity across the three groups,including bacteria producing ***-free analysis suggested punctual,yet pronounced differences in the metagenomic signature in the PD+RS ***-PD highlights that a prebiotic treatment with RS is safe and well-tolerated in *** stable alpha-diversity and beta-diversity alongside altered fecal butyrate and calprotectin concentrations call for long-term studies,also investigating whether RS is able to modify the clinical course of PD.

关键词： Parkinson’s disease Short-chain fatty acid microbiota metagenomics Intestinal inflammation

A New β-Estradiol-lnducible Vector Set that Facilitates Easy Construction and Efficient Expression of Transgenes Reveals CBL3-Dependent Cytoplasm to Tonoplast Translocation of CIPK5

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molecular Plant 2013年第6期6卷 1814-1829页

作者： Kathrin Schliicking Kai H. Edel maria m. Drerupa Philipp Koster Christian Eckert Leonie Steinhorst Rainer Waadt Oliver Batistic jorg Kudla Institut for Biologie und Biotechnologie der Pflanzen Universit~t MQnster Schlossplatz 4 48149 MQnster Gel'many _ . _ Present address: University of California San Diego Division of Biological Sciences Cell and Developmental Biology Section 9500 Gilman Drive #0116 LaJolla CA 92093-0116 USA

Transient and stable expression of transgenes is central to many investigations in plant biology research. Chemical regulation of expression can circumvent problems of plant lethality caused by constitutive overexpression or allow inducible knock （out/down） approaches. Several chemically inducible or repressible systems have been described and successfully applied. However, cloning and application-specific modification of most available inducible expression systems have been limited and remained complicated due to restricted cloning options. Here we describe a new set of 57 vectors that enable transgene expression in transiently or stably transformed cells. All vectors harbor a synthetically optimized XVE expression cassette, allowing I^-estradiol mediated protein expression. Plasmids are equipped with the reporter genes GUS, GFP, mCherry, or with HA and Strepll epitope tags and harbor an optimized multiple cloning site for flexible and simple clon- ing strategies. moreover, the vector design allows simple substitution of the driving promoter to achieve tissue-specificity or to modulate expression ranges of inducible transgene expression. We report details of the kinetics and dose-dependence of expression induction in Arabidopsis leaf mesophyll protoplasts, transiently transformed Nicotiana benthamiana leaves, and stably transformed Arabidopsis plants. Using these vectors, we investigated the influence of CBL （Calcineurin B-like） protein expression on the subcellular localization of CIPKs （Calcineurin B-like interacting protein kinases）. These analyses uncovered that induced co-expression of CBL3 is fully sufficient for dynamic translocation of CIPK5 from the cytoplasm to the tonoplast. Thus, the vector system presented here facilitates a broad range of research applications.

关键词： vector inducible gene expression βestradiol Arabidopsis CBL CIPK.

First record of stable isotopes(δ^(13)C, δ^(18)O) and element ratios(mg/Ca, Sr/Ca) of middle to Late jurassic belemnites from the Indian Himalayas and their potential for palaeoenvironmental reconstructions

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journal of Palaeogeography 2021年第3期10卷 416-437页

作者： matthias Alberti Franz T.Fursich Dhirendra K.Pandey Nils Andersen Dieter Garbe-Schonberg Suraj Bhosale Ketan Chaskar jorg m.Habermann State Key Laboratory for mineral Deposits Research School of Earth Sciences and EngineeringCenter for Research and Education on Biological Evolution and Environment and Frontiers Science Center for Critical Earth Material CyclingNanjing UniversityNanjing 210023Jiangsu ProvinceChina GeoZentrum Nordbayern Friedrich-Alexander-Universität Erlangen-NürnbergLoewenichstraße 2891054 ErlangenGermany Department of Geology School of EarthBiological and Environmental ScienceCentral University of South Bihar SH 7Gaya-Panchanpur RoadVillage KarharaPost FatehpurGaya 824236(Bihar)India Department of Earth and Environmental Science KSKV Kachchh UniversityBhujIndia Leibniz Laboratory for Radiometric Dating and Stable Isotope Research Christian-Albrechts-Universität zu KielMax-Eyth-Straße 1124118 KielGermany Institut für Geowissenschaften Christian-Albrechts-Universität zu KielLudewig-Meyn-Straße 1024118 KielGermany

middle to Late jurassic belemnites from the Spiti and Zanskar valleys in the Indian Himalayas were used for stable isotope(δ^(13)C, δ^(18)O) and element(mg/Ca, Sr/Ca) analyses. Although the Himalayan orogeny deformed and altered a large portion of the collected fossils, cathodoluminescence and scanning electron microscopy in combination with analyses of iron and manganese contents allowed the identification of belemnites believed to still retain their original chemical composition. Results indicate a long-term temperature decrease from the middle Callovian–Oxfordian to the Tithonian, which is proposed to have been caused by a concomitant drift of eastern Gondwana into higher palaeolatitudes. Reconstructed absolute temperatures depend on the used equation and assumed δ^(18)O value of seawater, but most likely varied between 17.6 ℃ to 27.6℃ in the Kimmeridgian and Tithonian with average values between 22 ℃ to 24 ℃. This way, temperatures were similar to slightly warmer than today at comparable latitudes. The reconstruction of absolute temperatures for the middle Callovian–Oxfordian was hindered by a larger number of poorly preserved belemnites representing this time interval.

关键词： jurassic Palaeoclimate Water temperature Spiti Zanskar India Tethyan Himalaya Eastern Gondwana

Effect of toasting grain silages from field peas(Pisum sativum)and field beans(Vicia faba)on in vitro gas production,methane production,and post-ruminal crude protein content

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Animal Nutrition 2020年第3期6卷 342-352页

作者： martin Bachmann Christian Kuhnitzsch Sebastian michel Annabel Thierbach mandy Bochnia jorg m.Greef Siriwan D.martens Olaf Steinhofel Annette Zeyner Institute of Agricultural and Nutritional Sciences Martin Luther University Halle-WittenbergHalle(Saale)06120Germany Saxon State Office for Environment Agriculture and GeologyKollitsch04886Germany julius Kühn Institute Federal Research Centre for Cultivated PlantsInstitute for Crop and Soil ScienceBraunschweig38116Germany

Legume grains such as field peas and field beans can be produced on a local level,and may be reliable sources of dietary protein and energy apart from common soybean and rapeseed *** ruminants,protein,starch,and carbohydrates from peas and field beans are fermented in large part before reaching the small *** objective of this study was to evaluate the effects of a combination of ensiling and hydro-thermic treatment(i.e.,toasting at 160℃for 30 min)of grains of peas and field beans on the concentrations of post-ruminal crude protein(PRCP)and rumen-undegraded protein(RUP).moreover,24-h gas production and methane production were *** this,an in vitro batch culture system with ruminal fluid from sheep was ***-undegraded protein was determined using the Streptomyces griseus protease *** electron micrographs were used to visualize morphological changes of starch granules and their joint matrices in peas and field beans after ensiling,toasting,or a combination of *** pea grains contained crude protein(CP)at 199 g/kg Dm,PRCP at 155 g/kg Dm at a ruminal passage rate of 0.08/h(Kp8),RUP at 33 g/kg Dm at Kp8,and starch at 530 g/kg *** field beans contained CP at 296 g/kg Dm,PRCP at 212 g/kg Dm at Kp8,RUP at 54 g of/kg Dm at Kp8,and starch at 450 g/kg *** PRCP did not considerably differ among native and treated peas or field *** in the peas,RUP at Kp8 increased after ensiling by 10 g/kg Dm(i.e.,30%;Pmethane production were not affected by any treatment,and scanning electron micrographs did not reveal structural changes on the starches doubtless of any *** seemed to be more affected by treatment with ensiled+toasted peas than with ensiled+toasted field beans,but starches and other carbohydrates from both legumes remained unaffec

关键词： Legume grain silage Toasting In vitro gas production Protein evaluation Streptomyces griseus protease test

Serum Concentrations of Tumor Necrosis Factor-α and its Soluble Receptors in Soldiers with and Without Combat-related Posttraumatic Stress Disorder： Influence of Age and Body mass Index

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Chinese medical journal 2016年第6期129卷 751-752页

作者： Hubertus Himmerich jorg-Egbert Wolf Peter Zimmermann Antje H Biihler Lesca m Holdt Daniel Teupser Kenneth C Kirkby Gerd D Willmund Ulrich Wesemann Department of Psychiatry Psychotherapy and Psychotraumatology Bundeswehr Hospital Berlin Germany Department of Psychological medicine King's College London London UK Department of Psychiatry and Psychotherapy University Hospital Leipzig Leipzig Germany Department of Laboratory medicine Ludw g-Max m ans-University Munich Germany Department of Psychiatry University of Tasmania Hobart Tasmania Australia

To the Editor： With great interest we read the original article of Chert et al published in the Chinese medical journal comparing serum concentrations of cytokines in patients of Han and Li ethnicity with posttraumatic stress disorder （PTSD）. The authors found significantly higher serum concentrations of the cytokines interleukin （IL）-2, IL-6, IL-8, and tumor necrosis factor （TNF）-α in PTSD patients compared to normal controls, and differences in cytokine concentrations according to ethnicity.

关键词：

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molecular Plant 2012年第4期5卷 951-954页

作者： Giuseppe Ortu Raffaella Balestrini Patricia A. Pereira jorg D. Becker Helge Kuste Paola Bonfante Department of Life Science and Systems Biology University of Torino Italy Istituto per la Protezione delle Piante--CNR Torino Italy Instituto Gulbenkian de Ciencia 2780-156 Oeiras Portugal Institut for Pflanzengenetik Leibniz Universitat Hannover Herrenhauser Str. 2 D-30419 Hannover Germany

Dear Editor, Phytohormones are essential regulators of plant develop- ment, but their role in the signaling processes between plants and fungi during arbuscular mycorrhizal （Am） establishment is far from being understood （Ludwig-m011er, 2010）. Am coloni- zation leads to extensive effects on host metabolism, as revealed by transcriptome studies of Am plants （Hogekamp et al., 2011）. Some genes have been specified as an Am core set, since they are mycorrhizal-responsive, irrespective of the identity of the plant,

关键词： Am真菌植物激素相关基因早期阶段调节过程生物合成相互作用赤霉素

利用Nd:YAG激光诱导击穿光谱法检测橡胶共混物中锌的均匀性分布

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中国光学 2015年第4期8卷 596-602页

作者： EmDE Benjamin HERmSDORF jorg KAIERLE Stefan OVERmEYER Ludger 汉诺威激光中心

激光诱导击穿光谱是一种基于激光的定性、定量分析化学元素的方法,可用于分析混合室中的橡胶共混物。本文采用波长为1 064 nm、脉冲能量为100 m j的Nd∶YAG激光诱导击穿光谱法检测橡胶共混物中锌的分散情况。实验表明,正确混料决定了橡... 详细信息

激光诱导击穿光谱是一种基于激光的定性、定量分析化学元素的方法,可用于分析混合室中的橡胶共混物。本文采用波长为1 064 nm、脉冲能量为100 m j的Nd∶YAG激光诱导击穿光谱法检测橡胶共混物中锌的分散情况。实验表明,正确混料决定了橡胶产品的质量,较短的混合时间将会导致混料的不均匀分散;焦距等参数设置对光谱线强度影响较大。激发标定样品可产生标定曲线。通过行扫描可分析锌在橡胶共混物中的分散情况。通过估算沿着行扫描方向的强度分布,可检测由于混合时间较短和旋转速度不同而引起的锌均匀性变化。

关键词：激光诱导击穿光谱橡胶 Nd∶YAG激光器锌