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Improved resolution of mixed STR profiles using a fully automated differential cell lysis/DNA extraction method

Forensic Sciences Research 2020年第2期5卷 106-112页

作者： Matthew C.Goldstein jordan o.cox Lori B.Seman Tracey Dawson Cruz Department of Forensic Science Virginia Commonwealth UniversityRichmondVirginiaUSA

Sexual assault evidence often contains sperm cells,which are typically separated from nonsperm cells using manual differential lysis *** goal of this study was to evaluate the automated QIAGEN QIAcube for this purpose and to compare it to manual QIAGEN and manual organic differential methods using DNA yields and STR profile data for *** yields were determined by qPCR,followed by multiplex STR amplification,CE analysis,and mixture *** automated method was capable of effective cell separation,producing DNA yields sufficient for STR ***,sperm fraction human:male DNA ratios from the QIAcube samples were consistently closer to the desired 1:1 and STR profiles were less likely to result in mixtures,with 6–8fewer female alleles detected(median 1.5 alleles).Ultimately,using the QIAcube for automated differential processing of semen-containing mixtures reduces the need for downstream mixture interpretation and improves STR profile quality with substantially less hands-on time.

关键词： Forensic sciences forensic genetics sexual assault evidence QIAcube differential lysis sperm lysis automated DNA extraction mixture analysis

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Effects of storage time on DNA profiling success from archived latent fingerprint samples using an optimised workflow

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Forensic Sciences Research 2022年第1期7卷 61-68页

作者： Sydney I.Menchhoff April D.Solomon jordan o.cox Madison E.Hytinen Marilyn T.Miller Tracey Dawson Cruz Department of Forensic Science Virginia Commonwealth UniversityRichmondVirginiaUSA Jefferson Parish Sheriff’s office Regional DNA Laboratory HarveyLouisianaUSA

Due to recent improvements in forensic DNA testing kit sensitivity,there has been an increased demand in the criminal justice community to revisit past convictions or cold *** of these cases have little biological evidence other than touch DNA in the form of archived latent fingerprint lift *** this study,a previously developed optimised workflow for this sample type was tested on aged fingerprints to determine if improved short tandem repeat(STR)profiles could be ***-year-old samples processed with the optimised workflow produced an average of approximately five more STR alleles per profile over the traditional *** optimised workflow also produced detectable alleles in samples aged out to 28 *** the methods tested,the optimised workflow resulted in the most informative profiles from evidence samples more representative of the forensic *** workflow is recommended for use with archived latent fingerprint samples,regardless of the archival time.

关键词： Forensic sciences forensic genetics low template DNA touch DNA aged samples archived latent fingerprints optimised workflow

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The Emergence of Rapid Counter Immunostaining in the Controlled Narrow Excision of Malignant Melanoma—How We Do It

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Journal of Cancer Therapy 2013年第6期4卷 1082-1084页

作者： jordan Troxel Grace Brummer Kristin cox S. Ray Peterson Department of Dermatology Central Utah Clinic Provo USA

Mohs Micrographic Surgery (MMS) is widely employed in the treatment of non-melanoma skin cancer and is a preferred treatment for many cutaneous malignancies, particularly in high risk locations and tumors [1,2]. It has also been used in the narrow excision of malignant melanoma with local control rates equivalent to standard margins [3]. It has gained acceptance in the treatment of noninvasive melanoma where standard 0.5 cm margins may be inadequate for local control [4]. The frozen section processing used in MMS has been assumed by some to be inadequate in assessing melanocyte populations or residual melanoma within excision margins. This difficulty has likely led to a majority of surgeons with fellowship training to process margins with slow, permanent hematoxylin and eosin sections (“slowmohs”) or to simply resort to standard 0.5, 1.0, or 2.0 cm margins with traditional excision and outside pathology confirmation of clear margins. A recent survey of practicing fellowship-trained Mohs surgeons revealed roughly one-third (35.9%) of Mohs surgeons felt comfortable interpreting MART-1 immunostains, and far fewer were actually performing immunostains in their labs [5]. Some Mohs surgeons currently refer melanoma to a colleague experienced in processing and reading melanoma with available rapid immunostaining. The development of rapid immunohistochemistry, which can be implemented into a traditional frozen section laboratory, has greatly improved the ease of interpreting margins in the excision of melanoma. Although the process is considerably more complicated than staining with H&E or Toluidine Blue (T-Blue), it easily falls within the skill-set and equipment of most busy frozen section laboratories. The additional cost of biologic reagents may be fully recovered by proper billing of immunohistochemical laboratory work and interpretation of slides.

关键词： Moh’s Surgery Malignant Melanoma Rapid MART-1

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Weight loss increases skeletal muscle mitochondrial energy efficiency in obese mice

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Life Metabolism 2023年第2期2卷 23-34页

作者： Patrick J.Ferrara Marisa J.Lang jordan M.Johnson Shinya Watanabe Kelsey L.McLaughlin John Alan Maschek Anthony R.P.Verkerke Piyarat Siripoksup Amandine Chaix James E.cox Kelsey H.Fisher-Wellman Katsuhiko Funai Diabetes&Metabolism Research Center University of UtahSalt Lake CityUtah 84112United States Department of Nutrition&Integrative Physiology University of UtahSalt Lake CityUtah 84112United States East Carolina Diabetes&obesity Institute East Carolina UniversityGreenvilleNorth Carolina 27834United States Department of Physiology East Carolina UniversityGreenvilleNorth Carolina 27834United States Metabolomics Core Research Facility University of UtahSalt Lake CityUtah 84112United States Molecular Medicine Program University of UtahSalt Lake CityUtah 84112United States Department of Biochemistry University of UtahSalt Lake CityUtah 84112United States

Weight loss from an overweight state is associated with a disproportionate decrease in whole-body energy expenditure that may contribute to the heightened risk for weight *** suggests that this energetic mismatch originates from lean *** this phenomenon is well documented,the mechanisms have remained *** hypothesized that increased mitochondrial energy efficiency in skeletal muscle is associated with reduced expenditure under weight ***(WT)male C57BL6/N mice were fed with high-fat diet for 10 weeks,followed by a subset of mice that were maintained on the obesogenic diet(oB)or switched to standard chow to promote weight loss(WL)for additional 6 *** energy efficiency was evaluated using high-resolution respirometry and *** spectrometric analyses were employed to describe the mitochondrial proteome and *** loss promoted~50%increase in the efficiency of oxidative phosphorylation(ATP produced per o_(2) consumed,or P/o)in skeletal ***,Weight loss did not appear to induce significant changes in mitochondrial proteome,nor any changes in respiratory supercomplex ***,it accelerated the remodeling of mitochondrial cardiolipin(CL)acyl-chains to increase tetralinoleoyl CL(TLCL)content,a species of lipids thought to be functionally critical for the respiratory *** further show that lowering TLCL by deleting the CL transacylase tafazzin was sufficient to reduce skeletal muscle P/o and protect mice from diet-induced weight *** findings implicate skeletal muscle mitochondrial efficiency as a novel mechanism by which weight loss reduces energy expenditure in obesity.

关键词： energy efficiency energy expenditure mitochondria oxidative phosphorylation phospholipids weight loss

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