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Induced Monocytes-Derived HSCs (CD34+) with LPS Accelerated Homing Rat Bone Marrow-Mesenchymal Stem Cell (BM-MSCs, CD105) in Injured Pancreas

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Journal of Biomedical Science and Engineering 2015年第5期8卷 333-344页

作者： Fedik A. Rantam Purwati Budi Setiawan Sony Wibisono Ferdiansyah joni wahyuhadi Edward Mouli Dwikora N. Utomo Heri Suroto Candra Bumi Stem Cell Laboratory Institute of Tropical Disease Airlangga University Surabaya Indonesia Virology and Immunology Laboratory Department of Microbiology Faculty of Veterinary Medicine Airlangga University Surabaya Indonesia Tropic and Infection Division of Internal Medicine School of Medicine Airlangga University Surabaya Indonesia Animal Surgery Animal Hospital Faculty of Veterinary Medicine Airlangga University Surabaya Indonesia Regenerative Medicine Department of Orthopedic Dr. Soetomo Teaching Hospital/School of Medicine Airlangga University Surabaya Indonesia Department of Neurosurgery Dr. Soetomo Teaching Hospital/School of Medicine Airlangga University Surabaya Indonesia

Investigating the function of combining induced rat monocytes-derived bone marrow-haemopoietic stem cell (rat BM-HSCs) with LPS and rat bone marrow-mesenchymal stem cell (rat BM-MSCs) was to analyze the acceleration of homing process mechanism in injured pancreas. Mononucleated stem cells were isolated from aspirated whole rat BM using ficoll and cultured in α-MEM complete growth medium in 10 cm petridish. After two days, adherent cells after washing twice in petridish were added α-MEM growth medium and then mesenchymal cells were characterized using CD105 marker in third passage and labeled PKH26. Then haemopoietic stem cells (HSCs) were isolated with magnetic beads CD34+ and differentiated in vitro, and then induced monocytes with LPS. Animal experiment used 28 male Wistar rats, and divided them into 4 groups. After transplantation combined, both cells between monocyte derived HSc (mHSCs) and rat BM-MSC were analyzed expression of pair box gen 4 (Pax4), pancreatic and duodenal homeobox (Pdx1), C-peptide using immunohistochemistry, then secretion of insulin and C-peptide analyzed using indirect ELISA. Results showed that the expressions of Pax4, Pdx1, C-peptide found in the surface membrane cell of pancreatic cell, and secreted C-peptide and insulin were shown significant (P < 0.05) in transplanted group 2, 3 and 4, but in group 3 were transplanted with combined cells more dominant than non-combined cells. Conclusions suggested that combining of induced monocytes-derived HSCs and rat BM-MSCs has accelerated homing MSCs into injured pancreatic tissue.

关键词： Induced Monocyte Derived HSCs Rat BM-MSCs Homing Injured Pancreas

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