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Fluorescent nanoswitch for monitoring specific pluripotency-related microRNAs of induced pluripotent stem cells： Development of polyethyleneimine- oligonucleotide hybridization probes

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Nano Research 2017年第8期10卷 2545-2559页

作者： Seungmin Han Hye Young Son Byunghoon Kang Eunji Jang Jisun Ki Na Geum Lee jongjin park Moo-Kwang Shin Byeonggeol Mun Jeong-Ki Min Seungjoo Haam Department of Chemical and Biomolecular Engineering College of Engineering Yonsei University Seou1120-749 Republic of Korea Department of Radiology College of Medicine Yonsei University Seou1120-752 Republic of Korea Severance Biomedical Science Institute College of Medicine Yonsei University Seou1120-752 Republic of Korea Biotherapeutics Translational Research Center Korea Research Institute of Bioscience and Biotechnology Daejeon 34141 Republic of Korea Department of Biomolecular Science University of Science & Technology Daejeon 34113 Republic of Korea

The isolation of high-grade （i.e. high-pluripotency） human induced pluripotent stem cells （hiPSCs） is a decisive factor for enhancing the purity of hiPSC populations or differentiation efficiency. A non-invasive imaging system that can monitor microRNA （miRNA） expression provides a useful tool to identify and analyze specific cell populations. However, previous studies on the monitoring/isolation of hiPSCs by miRNA expression have limited hiPSCs＇ differentiation system owing to long-term incubation with miRNA imaging probe-nanocarriers. Therefore, we focused on monitoring high-grade hiPSCs without influencing the pluripotency of hiPSCs. We reduced nanoparticle transfection time, because hiPSCs are prone to spontaneous differentiation under external factors during incubation. The fluorescent nanoswitch （＂ON＂ with target miRNA）, which can be applied for either imaging or sorting specific cells by fluorescence signals, contains an miRNA imaging probe （miP） and a PEI-PEG nanoparticle （miP-P）. Consequently, this nanoswitch can sense various endogenous target miRNAs within 30 min in vitro, and demonstrates strong potential for not only imaging but also sorting pluripotent hiPSCs without affecting pluripotency. Moreover, miP-P-treated hiPSCs differentiate well into endothelial cells, indicating that miP-P does not alter the pluripotency of hiPSCs. We envisage that this miRNA imaging system could be valuable for identifying and sorting high-grade hiPSCs for improved practical applications.

关键词： induced pluripotent stem cells (iPSCs) pluripotency microRNA (miRNA) fluorescence imaging nanoparticle

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Ageing Behavior of Mg-xAl-Zn with Variation of Al Content

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Journal of Materials Science & Technology 2008年第1期24卷 51-53页

作者： Byoungho Kim jongjin Jeon Yeongdo park Yongho park Ikmin park Department of Materials Science and Engineering Pusan National University 30 Jangjeon-dongGeumjeong-guBusan 609-735Korea Department of Advanced Materials Engineering Dong-Eui University 995 EomgwangnoJin-guBusan 614-714Korea

The ageing behavior of Mg-xAl-Zn alloys with a variation of AI contents （x=3,5,7,9 and 11 wt pct） were *** solution treatment and artificial ageing were carried out at 693 and 493 K,respectively. After the solid solution treatment,β-Mg-（17）Al-（12） was resolved to theα-Mg matrix in the specimens which contained Al up to 9 wt pct and partially resolved in the specimen of 11 wt pct *** ageing,theβ- Mg-（17）Al-（12） phase was precipitated at the grain boundary in the form of discontinuous precipitation and grown into the *** volume fraction ofβ-phase was increased with the Al *** strength increased with the volume fraction of precipitatedβ-phase,which was believed to strengthen the matrix.

关键词： Ageing Mg alloy Discontinuous precipitation

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