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Determination of Rutin Using Lucigenin as Chemiluminogenic Reagent

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Determination of Rutin Using Lucigenin as Chemiluminogenic R...

Converting textile waste into value-added chemicals:An integrated bio-refinery process

The 3rd Asian and Oceanian Conference on Photobiology

作者： Seung Oh Jin Kyoung Hye Choi Yeoun suk Suh Sang Hak Lee jong Ha Choi Chang Jin Kim Department of Chemistry Kyungpook National UniversityTaegu702-701Korea Department of Chemistry Kyungpook National UniversityTaegu702-701Korea Department of Chemistry Kyungpook National UniversityTaegu702-701Korea Department of Chemistry Kyungpook National UniversityTaegu702-701Korea Department of Chemistry Andong National UniversityAndong760-749Korea Department of Chemistry Daegu University Taegu 705-033 Korea

＜正＞Rutin, a flavanoid glucoside known as vitamin P, is an oral capillary preservatory drug, usually used for the therapy of chronic venous insufficiency. An inhibitory effect of rutin on the chemiluminescence （CL） reaction between lucigenin and hydrogen peroxide was reported, and this effect was used for the determination of rutin in medicine. The CL signal produced by the reaction between lucigenin and H2O2, was decreased in the presence of rutin. Rutin was sensed by measuring the decrement of CL intensity, and which was observed to be linear over 1×10-6M - 1×10-4M rutin concentration range, and the limit of detection was 2.5×10-7M. The concentrations of lucigenin, H2O2, NaOH and flow rate of the manifold were all optimized. The method proposed was applied successfully in the determination of rutin in pharmaceutical preparations .

关键词： Rutin Lucigenin Chemiluminescence

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Environmental Science and Ecotechnology 2023年第3期15卷 66-76页

作者： Eun Jin Cho Yoon Gyo Lee Younho song Ha Yeon Kim Dinh-Truong Nguyen Hyeun-jong Bae Bio-Energy Research Center Chonnam National UniversityGwangju500-757Republic of Korea Department of Bioenergy Science and Technology Chonnam National UniversityGwangju500-757Republic of Korea School of Biotechnology Tan Tao UniversityLong An82000Viet Nam

The rate of textile waste generation worldwide has increased dramatically due to a rise in clothing consumption and ***,conversion of cotton-based,colored cotton-based,and blended cotton-polyethylene terephthalate(PET)textile waste materials into value-added chemicals(bioethanol,sorbitol,lactic acid,terephthalic acid(TPA),and ethylene glycol(EG))via enzymatic hydrolysis and fermentation was *** order to enhance the efficiency of enzymatic saccharification,effective pretreatment methods for each type of textile waste were developed,respectively.A high glucose yield of 99.1%was obtained from white cotton-based textile waste after NaOH ***,the digestibility of the cellulose in colored cotton-based textile wastes was increased 1.38e1.75 times because of the removal of dye materials by HPAC-NaOH *** blended cottonPET samples showed good hydrolysis efficiency following PET removal via NaOHeethanol pretreatment,with a glucose yield of 92.49%.The sugar content produced via enzymatic hydrolysis was then converted into key platform chemicals(bioethanol,sorbitol,and lactic acid)via fermentation or *** maximum ethanol yield was achieved with the white T-shirt sample(537 mL/kg substrate),which was 3.2,2.1,and 2.6 times higher than those obtained with rice straw,pine wood,and oak wood,*** was selectively converted into sorbitol and LA at a yield of 70%and 83.67%,*** and EG were produced from blended cottonPET via NaOHeethanol *** integrated biorefinery process proposed here demonstrates significant potential for valorization of textile waste.

关键词： Textile waste Value-added biochemicals Integrated biorefinery Bioethanol Lactic acid

Aberrant expression of krUppel-like factor 6 protein in colorectal cancers

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World Journal of Gastroenterology 2006年第14期12卷 2250-2253页

作者： Yong-Gu Cho Byung-Jun Choi Jae-Whie song Su-Young Kim suk-Woo Nam Sug-Hyung Lee Nam-Jin Yoo Jung-Young Lee Won-Sang Park Department of Pathology College of Medicine The Catholic University of Korea505 Banpo-dongSeocho-guSeoul 137-701Korea Department of Pathology College of Medicine The Catholic University of Korea505 Banpo-dongSeocho-guSeoul 137-701Korea Department of PediatricsCollege of MedicineThe Catholic University of Korea505 Banpo-dongSeocho-guSeoul 137-701Korea

AIM： TO investigate whether krüppel-like factor 6 （KLF6） plays an important role in the development and/or progression of colorectal cancer. METHODS： A total of 123 formalin-fixed and paraffinembedded colorectal cancer specimens were analyzed by immunohistochemistry using tissue microarray for the expression of KLF6 protein. The specimens were collected over a 3-year period in the laboratories at our large teaching hospital in Seoul, Republic of Korea. The correlation of KLF6 expression with clinicopathologic parameters was analyzed by χ^2 test and Bartholomew test. RESULTS： Normal colonic epithelium showed weak to moderate expression of KLF6, whereas reduced KLF 6 expression or loss of KLF6 expression was seen in 45 （36.6%） of the 123 colorectal carcinoma specimens. Interestingly, aberrant expression of KLF6 was detected in 25 （43.1%） of 58 cases with metastasis to regional lymph node and in 31 （47.0%） of 66 tumors more than 5 cm in size. Statistically, loss of KLF6 expression was significantly associated with tumor size （P〈0.05）. However, there was no significant correlation between KLF6 expression and Dukes＇ stage （Bartholomew test, P〉 0.05）, tumor location and lymph node metastasis （χ^2 test, P〉0.05）.CONCLUSION： Loss of KLF6 expression may be a common and early event in colorectal carcinogenesis.

关键词： KLF6 Mutation Immunohistochemistry Aggressiveness Tumor stage Colorectal cancer

Optimum Current Distribution on Resistive Strip for Arbitrarily Prescribed RCS Pattern

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Optimum Current Distribution on Resistive Strip for Arbitrar...

Effect of Autologus Platelet-Rich Plasma on IL-6, MMP-3 and MCP-1 Expression in Synoviocytes from Osteoarthritic Patients Knees

1998 International Conference on Microwave and Millimeter Wave Technology

作者： jong-In Choi Byoung-Hee Lee Woo-Jun Yang Kwon-Sik song Eui-Joon Park Kumoh National University of Technology（188 Shinpyung KumiKyungpookKorea）

ISBN: (纸本)0780343085

＜正＞The desired far-zone scattered field from a resistive strip is determined by synthesizing the distribution of the induced surface current density in the strip using an optimization process. It may be advantageous to automatically control RCS levels of the main lobe and the *** synthesis is resulted in the appropriate resistance distribution varying transversely on the *** optimization is performed to control inherent null points in RCS pattern formula equivalent to Taylor’s space factor.

关键词： current distribution current density antenna theory radar cross-sections electromagnetic wave scattering antenna radiation patterns optimum current distribution resistive strip arbitrarily prescribed RCS pattern far-zone scattered field induced surface current density distribution optimization process resistance distribution null points control

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Open Journal of Regenerative Medicine 2014年第3期3卷 64-72页

作者： Chang Ich Hur Cheol Park Hanlou Li jong Keun Seon Hyun Keun Kim Taek Rim Yoon Eun-Kyoo song Center for Joint Disease Chonnam National University Hwasun Hospital Jeonnam South Korea Fraunhofer Institute for Cell Therapy and Immunology Leipzig Germany Cardiovascular Convergence Research Center of Chonnam National University Gwangju South Korea

Nowadays, some studies reported promising results of platelet-rich plasma (PRP) for the treatment of osteoarthritis (OA). However, the effects of PRP on prevention of osteoarthritis in knee joints have been debated. The present study investigated the effects of PRP on osteoarthritis related inflammatory cytokines expressed in fibroblast-like synoviocytes (FLS) from osteoarthritic knees. The synovial tissues were harvested from eight osteoarthritic patients who had undertaken total knee arthroplasties (TKAs) and cultured in DMEM containing 10% FBS. Platelet-rich plasma releasate (PRPr) was made by clotting or activation of PRP by citrate. The levels of PDGF-AA and VEGF in PRPr and whole blood were measured with ELISA method. The FLS were isolated and cultured from osteoarthritic knees. The IL-1β stimulated FLS were cultivated with three different conditions (none, 1% and 10% of PRP). To determine the expression of IL-6, MMP-3, and MCP-1, we used reverse transcriptase polymerase chain reaction (RT-PCR). The concentrations of platelet count in PRP were about 7 to 9 times higher than those of whole blood. The levels of PDGF-AA in PRPr were approximately 3 to 4 times higher than those of whole blood. The levels of VEGF in PRPr were also significantly 7 to 18 times higher than those of whole blood. Without induction of the FLS with IL-1β, 1% or 10% PRPr did not reduce expressions of inflammatory proteins (MMP-3, MCP-1), except for IL-6. However, with induction of the FLS with IL-1β, both concentrations (1% and 10%) of PRPr reduced significantly all inflammatory protein expressions (IL-6, MMP-3, MCP-1). PRPr diminished inflammatory IL-1β-mediated effects on human osteoarthritic fibroblast-like synoviocytes. These results suggest that platelet-rich plasma can be a good therapeutic option for the treatment of osteoarthritis.

关键词： Platelet-Rich Plasma Inflammatory Cytokines Synoviocyte Osteoarthritis

Superior gallstone dissolubility and safety of tert-amyl ethyl ether over methyl-tertiary butyl ether

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World Journal of Gastroenterology 2019年第39期25卷 5936-5952页

作者： Dong Do You suk Joon Cho Ok-Hee Kim Jin Sook song Kyu-Seok Hwang Sang Chul Lee Kee-Hwan Kim Ho Joong Choi Ha-Eun Hong Haeyeon Seo Tae Ho Hong Jung Hyun Park Tae Yoon Lee Joseph Ahn Jae-Kyung Jung Kwan-Young Jung Say-June Kim Department of Surgery St.Vincent's HospitalCollege of MedicineThe Catholic University of KoreaSeoul 16247South Korea College of Pharmacy Chungbuk National UniversityCheongju 28644South Korea Department of Surgery Seoul St.Mary’s HospitalCollege of Medicinethe Catholic University of KoreaSeoul 06591South Korea Catholic Central Laboratory of Surgery Institute of Biomedical IndustryCollege of Medicinethe Catholic University of KoreaSeoul 06591South Korea Bio and Drug Discovery Division Korea Research Institute of Chemical TechnologyDaejeon 34114South Korea Department of Surgery Daejeon St.Mary's HospitalCollege of Medicinethe Catholic University of KoreaSeoul 34943South Korea Department of Surgery Uijeongbu St.Mary's HospitalCollege of Medicinethe Catholic University of KoreaSeoul 11765South Korea Department of Biomedicine and Health Science College of Medicinethe Catholic University of KoreaSeoul 06591South Korea Department of Surgery Eunpyeong St.Mary’s HospitalCollege of Medicinethe Catholic University of KoreaSeoul 03312South Korea Department of Medicinal Chemistry and Pharmacology University of Science and TechnologyDaejeon 34113South Korea

BACKGROUND The use of methyl-tertiary butyl ether(MTBE)to dissolve gallstones has been limited due to concerns over its toxicity and the widespread recognition of the safety of laparoscopic *** adverse effects of MTBE are largely attributed to its low boiling point,resulting in a tendency to ***,if there is a material with a higher boiling point and similar or higher dissolubility than MTBE,it is expected to be an attractive alternative to *** To determine whether tert-amyl ethyl ether(TAEE),an MTBE analogue with a relatively higher boiling point(102°C),could be used as an alternative to MTBE in terms of gallstone dissolubility and *** The in vitro dissolubility of MTBE and TAEE was determined by measuring the dry weights of human gallstones at predetermined time intervals after placing them in glass containers with either of the two *** in vivo dissolubility was determined by comparing the weights of solvent-treated gallstones and control(dimethyl sulfoxide)-treated gallstones,after the direct infusion of each solvent into the gallbladder in both hamster models with cholesterol and pigmented *** The in vitro results demonstrated a 24 h TAEE-dissolubility of 76.7%,56.5%and 38.75%for cholesterol,mixed,and pigmented gallstones,respectively,which represented a 1.2-,1.4-,and 1.3-fold increase in dissolubility compared to that of *** the in vitro experiment,the 24 h-dissolubility of TAEE was 71.7%and 63.0%for cholesterol and pigmented gallstones,respectively,which represented a 1.4-and 1.9-fold increase in dissolubility compared to that of *** addition,the results of the cell viability assay and western blot analysis indicated that TAEE had a lower toxicity towards gallbladder epithelial cells than *** We demonstrated that TAEE has higher gallstone dissolubility properties and safety than those of *** such,TAEE could present an attractive alternative to MTBE if our findings

关键词： Tert-amyl ethyl ether Gallstones Methyl-tert-butyl ether Contact litholytic agent

Hepatic angiosarcoma manifested as recurrent hemoperitoneum

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World Journal of Gastroenterology 2008年第18期14卷 2935-2938页

作者： Seung-Woo Lee Chun-Young song Young-Hwa Gi Sang-Beom Kang Yon-Soo Kim Soon-Woo Nam Dong-Soo Lee jong-Ok Kim Division of Gasroenterology and Hepatology Departments of Internal Medicine The Catholic University of Korea College of Medicine Seoul 301-723 Korea Department of pathology The Catholic University of Korea College of Medicine Seoul 301-723 Korea

Angiosarcoma is a rare tumor that account for less than 1% of all sarcomas. Although hepatic angiosarcoma usually presents with unspecific symptoms, it rapidly progresses and has a high mortality. We report a rare cas... 详细信息

关键词： Angiosarcoma Hemoperitoneum Primary hepatic tumor

Diversity of bacterial and fungal strains isolated from coastal sand-dunes in Korea

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Diversity of bacterial and fungal strains isolated from coas...

Outcome of transarterial chemoembolization-based multi-modal treatment in patients with unresectable hepatocellular carcinoma

中国菌物学会2016年学术年会

作者： Minji Jeong Ki-Eun Lee Min-Kyeong Kim Kyoung-Mi Kim Hyun Kim Yoon-jong Nam Yoosun Oh Hyerim Choi Nan-Yeong Lee jong-Guk Kim Myung-suk Kang Department of Life Sciences and Biotechnology Institute for MicroorganismsKyungpook National University

＜正＞Microbial cultures were collected from roots of plants（11 species）in three coastal sand-dunes（Sinduri,Goraebul,Sowhang）in *** amplification of the 16S r RNA genes were conducted using the forward primer 27F and the reverse primer1492R and sequencing of the genes was performed by using Big Dye Terminator cycle Sequencing Ready Reaction Kit.160 isolates were identified:Sinduri coastal

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World Journal of Gastroenterology 2015年第8期21卷 2395-2404页

作者： Do Seon song Soon Woo Nam Si Hyun Bae Jin Dong Kim Jeong Won Jang Myeong Jun song Sung Won Lee Hee Yeon Kim Young Joon Lee Ho jong Chun Young Kyoung You jong Young Choi Seung Kew Yoon Department of Internal Medicine College of Medicine The Catholic University of Korea Department of Internal Medicine Cheju Halla General Hospital Department of Radiology College of Medicine The Catholic University of Korea Department of Surgery College of Medicine The Catholic University of Korea

AIM:To investigate the efficacy and safety of transarterial chemoembolization(TACE)-based multimodal treatment in patients with large hepatocellular carcinoma(HCC).METHODS:A total of 146 consecutive patients were included in the analysis,and their medical records and radiological data were reviewed ***:In total,119 patients received TACE-based multi-modal treatments,and the remaining 27 received conservative *** survival(P<0.001)and objective tumor response(P=0.003)were significantly better in the treatment group than in the conservative *** subgroup analysis,survival benefits were observed not only in the multi-modal treatment group compared with the TACE-only group(P=0.002)but also in the surgical treatment group compared with the loco-regional treatment-only group(P<0.001).Multivariate analysis identified tumor stage(P<0.001)and tumor type(P=0.009)as two independent pre-treatment factors for *** adjusting for significant pre-treatment prognostic factors,objective response(P<0.001),surgical treatment(P=0.009),and multi-modal treatment(P=0.002)were identified as independent post-treatment prognostic ***:TACE-based multi-modal treatments were safe and more beneficial than conservative *** surgery after successful downstaging resultedin long-term survival in patients with large,unresectable HCC.

关键词： Hepatocellular carcinoma Multimodal treatment Tran