检索结果-南通市图书馆

Neural Regeneration Research 2024年第7期19卷 1417-1418页

作者： jonathan Witton Erica S.Brady Michael T.craig Department of Clinical and Biomedical Sciences Faculty of Health and Life SciencesUniversity of ExeterExeterEnglandUK Gladstone Institute for Neurological Disease San FranciscoCAUSA School of Psychology and Neuroscience College of MedicalVeterinary and Life SciencesUniversity of GlasgowGlasgowScotlandUK

Dementia is a devastating syndrome characterized by memory problems,confusion,and behavior changes that prevent people from performing everyday *** is the leading cause of dependency and disability amongst older people,resulting in a global economic cost of ~$1.3 *** 58 million people are currently afflicted by dementia worldwide,with Alzheimer s disease(AD) causing most(60-70%) of these cases.

关键词： Alzheimer everyday performing

在线全文

学校读者我要写书评

暂无评论

采用随机试验确定何时监测新治疗方法的反应

英国医学杂志中文版 2008年第3期11卷 168-173页

作者： Katy J L Bell Les Irwig jonathan C craig Petra Macaskill 汪海波(译) Screening and Test Evaluation Program School of Public Health Edward Ford Building (A27) The University of Sydney NSW 2006 不详

监测治疗的初始反应在患者的临床管理中总是有帮助的吗？ Katy Bell和他的同事们建立了一个理论框架。

关键词：治疗方法监测治疗反应随机试验临床管理指标监测慢性病

诊断性试验和策略的证据质量和推荐强度的分级

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国循证医学杂志 2009年第5期9卷 503-508页

作者： Holger J Schünemann Andrew D Oxman Jan Brozek Paul Glasziou Roman Jaeschke Gunn E Vist John W Williams Jr Regina Kunz jonathan craig Victor M Montori Patrick Bossuyt Gordon H Guyatt 李晓黄程陈耀龙李幼平代表GRADE工作组

GRADE系统能对诊断性试验或策略的证据质量和推荐强度进行分级。本文旨在阐释在此过程中如何考虑患者的重要结局，

关键词：诊断性试验证据质量强度 E系统

Detection and quantitation of host cell proteins in monoclonal antibody drug products using automated sample preparation and data-independent acquisition LC-MS/MS

在线全文

学校读者我要写书评

暂无评论

Journal of Pharmaceutical Analysis 2021年第6期11卷 726-731页

作者： Lisa Strasser Giorgio Oliviero craig Jakes Izabela Zaborowska Patrick Floris Meire Ribeiro da Silva Florian Füssl Sara Carillo jonathan Bones Characterization and Comparability Laboratory NIBRT-National Institute for Bioprocessing Research and TrainingDublinA94 X099Ireland School of Chemical and Bioprocess Engineering University College DublinDublinD04 V1W8Ireland

Ensuring the removal of host cell proteins(HCPs) during downstream processing of recombinant proteins such as monoclonal antibodies(m Abs) remains a *** residual HCPs might affect product stability or safety,constant monitoring is required to demonstrate their removal to be below the regulatory accepted level of 100 ng/*** current standard analytical approach for this procedure is based on ELISA;however,this approach only measures the overall HCP ***,the use of orthogonal methods,such as liquid chromatography-mass spectrometry(LC-MS),has been established,as it facilitates the quantitation of total HCPs as well as the identification and quantitation of the individual HCPs *** the present study,a workflow for HCP detection and quantitation using an automated magnetic bead-based sample preparation,in combination with a data-independent acquisition(DIA) LC-MS analysis,was *** the same instrumental setup commonly used for peptide mapping analysis of m Abs allows for its quick and easy implementation into pre-existing workflows,avoiding the need for dedicated instrumentation or ***,quantitation of HCPs over a broad dynamic range was enabled to allow monitoring of problematic HCPs or to track changes upon altered bioprocessing conditions.

关键词： Data-independent acquisition Host cell proteins Critical quality attributes Liquid chromatography-mass spectrometry Monoclonal antibody Chinese hamster ovary cells

Comparing different domains of analysis for the characterisation of N-glycans on monoclonal antibodies

在线全文

学校读者我要写书评

暂无评论

Journal of Pharmaceutical Analysis 2020年第1期10卷 23-34页

作者： Sara Carillo Raquel Peerez-Robles craig Jakes Meire Ribeiro da Silva Silvia Millan Martín Amy Farrell Natalia Navas jonathan Bones Characterisation and Comparability Laboratory NIBRT e the National Institute for Bioprocessing Research and TrainingFoster AvenueMount MerrionBlackrockCo.DublinA94 X099Ireland Department of Analytical Chemistry Biohealth Research Institute(ibs.GRANADA)University of GranadaGranadaSpain School of Chemical and Bioprocess Engineering University College DublinBelfieldDublin 4D04 V1W8Ireland

With the size of the biopharmaceutical market exponentially increasing,there is an aligned growth in the importance of data-rich analyses,not only to assess drug product safety but also to assist drug development driven by the deeper understanding of structure/function *** monoclonal antibodies,many functions are regulated by N-glycans present in the constant region of the heavy chains and their mechanisms of action are not completely *** importance of their function focuses analytical research efforts on the development of robust,accurate and fast methods to support drug development and quality *** N-glycan analysis is considered as the gold standard for glycosylation characterisation;however,it is not the only method for quantitative analysis of glycoform *** this study,ten different analytical workflows for N-glycan analysis were compared using four monoclonal *** observing good comparability between the quantitative results generated,it was possible to appreciate the advantages and disadvantages of each technique and to summarise all the observations to guide the choice of the most appropriate analytical workflow according to application and the desired depth of data generated.

关键词： N-Glycans Biopharmaceuticals Monoclonal antibodies Intact mass analysis Mass spectrometry Native mass spectrometry Glycan analysis Peptide mapping Glycopeptide analysis

在线全文

学校读者我要写书评

暂无评论

全球新元古界的含油气系统：北非展现的潜力

石油地质科技动态 2010年第5期 1-25页

作者： jonathan craig（）朱起煌（译）

虽然在世界很多地方都存在产油气的、探明的和潜在的新元古界油气层带，但从油气远景看，对新元古界的了解仍相对较差。在大地构造、气候和含油气系统方面，新元古代到早寒武世时期可以分为三个阶段：（1）大约750Ma前的拉伸纪（Tonian... 详细信息

虽然在世界很多地方都存在产油气的、探明的和潜在的新元古界油气层带，但从油气远景看，对新元古界的了解仍相对较差。在大地构造、气候和含油气系统方面，新元古代到早寒武世时期可以分为三个阶段：（1）大约750Ma前的拉伸纪（Tonian）至早成冰世（Cryogenian）阶段，主要表现为罗迪（Rodinia）超级古陆的形成、稳定和初始破裂；（2）中成冰世至早埃迭卡拉世（Ediacaran）阶段（约750—600Ma），其中包括了全球性的两次重大冰川作用，即斯图特（Sturtian）冰川作用和马里诺（Marinoan）冰川作用；（3）中埃迭卡拉世至早寒武世阶段（约600Ma以后），所对应的是冈瓦纳超级古陆的形成和稳定。有越来越多的证据表明，中一晚新元古代（可至早古生代）许多富含有机质层位的沉积是由全球性的冰川后海平面强烈上升所引起的，而此前的冰川作用属于“雪球地球”（SnowballEarth）型，在较为局部的范围还伴有盆地的发育和裂谷断陷作用。对北非的野外考察包括了毛里塔尼亚、阿尔及利亚和马里的陶代尼（Taoudenni）盆地、摩洛哥的小阿特拉斯山脉（Anti—Atlas）以及利比亚的昔兰尼加（Cyrenaica）台地、库夫拉（Kuffa）盆地和迈尔祖格（Murzuk）盆地。这一考察增加了对油气储层、源岩和封盖层之间关系的了解，同时证实了潜在储层相的前寒武系叠层石碳酸盐岩层位的广泛分布。这本专题文集介绍了对前寒武系一早寒武统含油气层带在年代地层学、源岩分布与性能、储层性能的控制因素以及封盖层分布等方面的当前研究成果，研究地区遍及南非、北非、中东和印度次大陆。

关键词：含油气系统新元古界北非冰川作用晚新元古代早寒武世油气储层前寒武系

Eliciting conditioned taste aversion in lizards:Live toxic prey are more effective than scent and taste cues alone

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Integrative Zoology 2017年第2期12卷 112-120页

作者： Georgia WARD-FEAR Jai THOMAS jonathan K.WEBB David J.PEARSON Richard SHINE School of Life and Environmental Sciences University of SydneySydneyNew South WalesAustralia School of Veterinary and Life Sciences Murdoch UniversityWestern AustraliaAustralia School of Life Sciences University of Technology SydneyBroadwayNew South WalesAustralia Western Australian Department of Parks and Wildlife WanerooWestern AustraliaAustralia

Conditioned taste aversion(CTA)is an adaptive learning mechanism whereby a consumer associates the taste of a certain food with symptoms caused by a toxic substance,and thereafter avoids eating that type of ***,wildlife researchers have employed CTA to discourage native fauna from ingesting toxic cane toads(Rhinella marina),a species that is invading tropical *** this paper,we compare the results of 2 sets of CTA trials on large varanid lizards(“goannas,”Varanus panoptes).One set of trials(described in this paper)exposed recently-captured lizards to sausages made from cane toad flesh,laced with a nausea-inducing chemical(lithium chloride)to reinforce the aversion *** other trials(in a recently-published paper,reviewed herein)exposed free-ranging lizards to live juvenile cane *** effectiveness of the training was judged by how long a lizard survived in the wild before it was killed(fatally poisoned)by a cane *** stimuli elicited rapid aversion to live toads,but the CTA response did not enhance survival rates of the sausage-trained goannas after they were released into the *** contrast,the goannas exposed to live juvenile toads exhibited higher long-term survival rates than did untrained *** results suggest that although it is relatively easy to elicit short-term aversion to toad cues in goannas,a biologically realistic stimulus(live toads,encountered by free-ranging predators)is most effective at buffering these reptiles from the impact of invasive toxic prey.

关键词： conditioned taste aversion invasive species Rhinella marina Varanus conservation tropical Australia

Extremely low-volume, high-intensity interval training improves exercise capacity and increases mitochondrial protein content in human skeletal muscle

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Open Journal of Molecular and Integrative Physiology 2013年第4期3卷 202-210页

作者： Jasmin K. Ma Trisha D. Scribbans Brittany A. Edgett J. Colin Boyd craig A. Simpson jonathan P. Little Brendon J. Gurd Department of Emergency Medicine Kingston General Hospital Kingston Canada Department of Health and Social Development University of British Columbia Okanagan Kelowna Canada Department of Kinesiology Queen’s University Kingston Canada

Purpose: The purpose of the present study was to evaluate whole-muscle content of several proteins involved in the regulation of skeletal muscle mitochondrial protein content and anaerobic capacity following 4 weeks of extremely low-volume high-intensity interval training (HIT). Methods: Young, healthy, recreationally active adult males (n = 8) trained 4 times a week for 4 weeks on a cycle ergometer. Each session involved 4 min of total exercise comprised of eight 20 s intervals at ~170% of peak aerobic power separated by 10 s rest. Muscle biopsies were taken prior to (pre) and ~72 hrs post-training (post). Par- ticipants completed an incremental peak oxygen up- take (VO2peak) test and a Wingate test pre-, mid-, and post-training. Results: VO2peak was elevated (p p < 0.05) and post-training (pre: 40.5 ± 3.8 ml·kg-1·min-1, mid: 43.4 ± 2.5 ml·kg-1·min-1, post-: 47.2 ± 2.9 ml·kg-1·min-1). Wingate mean power also increased with training (pre-: 701.0 ± 73.0 W, mid-: 745.5 ± 73.3 W, post-: 786.8 ± 80.0 W). While maximal citrate synthase activity was unchanged, protein expression of the mitochondrial protein cytochrome c oxidase (COX) subunit I (+27%;p p p = 0.08) increased. Increases (p α (+19%), and nuclear PGC-1α (+46%) were also observed after 4 weeks of HIT. No changes were observed in the whole-muscle contents of PDHe1a, PDK4, SIRT1, mTOR, S6K1, MCT1, or PFK protein. Conclusions: These results demonstrate that several mitochondrial protein (but not citrate synthase activity), PGC-1α protein content, and exercise capacity can be improved in only 4 min of total training time per day, 4 days per wk using HIT cycle training.

关键词： PGC-1α Mitochondrial Biogenesis High-Intensity Interval Training SIRT1 Aerobic Fitness

Arecibo上空钠层和铁层共体积同步激光雷达观测及相关性分析

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Arecibo上空钠层和铁层共体积同步激光雷达观测及相关性分析

对多年冻土地区路基稳定性改善方法的效果和成本回顾

中国空间科学学会空间物理学专业委员会第十五届全国日地空间物理学研讨会

作者：岳显昌周起厚 Shikha Raizada craig Tepley jonathan Friedman 武汉大学电子信息学院 Electrical and Computer Engineering Department Miami UniversityOxfordOhioUSA

通过比较Arecibo观测站(18.3,66.75)激光雷达共体积观测的该区域上空中间层钠原子层和铁原子层数据,我们发现这两种金属原子的时变特征在各个高度上都具有高度的相关性:在层的顶部和底部区域是正相关,在二者中间区域呈现出负相关。我们...

通过比较Arecibo观测站(18.3,66.75)激光雷达共体积观测的该区域上空中间层钠原子层和铁原子层数据,我们发现这两种金属原子的时变特征在各个高度上都具有高度的相关性:在层的顶部和底部区域是正相关,在二者中间区域呈现出负相关。我们援引了动力学作用来解释钠层和铁层之间的这种特殊关系。研究表明,观测到的两种金属层之间的关系可以根据各层对于大气波动的响应来进行合理解释。

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

四川建材 2015年第1期41卷 101-108页

作者： jonathan D.Regehr craig A.Milligan Jeannette Montufar Marolo Alfaro 庞应刚中铁二院工程集团有限责任公司四川成都610031

本文回顾了在多年冻土区改善路基稳定性方法的效果和成本,是基于文献研究成果的综述。在冻土地基进行路堤加载或者其吸热特性降低时都会引起多年冻土地基的不稳定。研究了各种工程策略来减轻这种影响,从基本原理,效果,成本等总结了四种... 详细信息

本文回顾了在多年冻土区改善路基稳定性方法的效果和成本,是基于文献研究成果的综述。在冻土地基进行路堤加载或者其吸热特性降低时都会引起多年冻土地基的不稳定。研究了各种工程策略来减轻这种影响,从基本原理,效果,成本等总结了四种类型的方法,即控制路基融化、冷却路基、隔离路基和降低路基填充重量。文献表明,控制路基融化、隔离路基或降低路基填充重量不能逆转冻土地基的长期退化。通过路堤的空气对流、通风管道、热虹吸管、热量消耗或者综合这些措施对降低路堤温度、提高稳定性非常有效。地理位置、气候条件和施工材料等因素都会引起成本不同。资料显示进行正常的维护比进行路基降温更廉价,但某些环境下维护并不可行。

关键词：永冻土路堤稳定性路基铁路工程学严寒地区