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The Boston lymphatic center’s early experience with lymph node transplantation to the upper extremity

plastic and Aesthetic Research 2022年第1期9卷 553-563页

作者： Rosie Friedman Monica Morgenstern Valeria p.Bustos Aaron Fleishman Kathy Shillue Leo L.Tsai jonathan F.Critchlow Dhruv Singhal Division of plastic and Reconstructive Surgery Department of SurgeryBeth Israel Deaconess Medical CenterHarvard Medical SchoolBostonMA 02215USA Division of Magnetic Resonance Imaging Department of RadiologyBeth Israel Deaconess Medical CenterHarvard Medical SchoolBostonMA 02215USA Department of Surgery Beth Israel Deaconess Medical CenterHarvard Medical SchoolBostonMA 02215USA

Aim:Although vascularized lymph node transplantation(VLNT)has gained recognition as an effective treatment option for lymphedema,no consensus on the timing of transplant with other lymphatic procedures has been *** aim of this study is to describe our institutional experience with VLNT,including our staged approach and report postoperative ***:A retrospective review of patients who underwent VLNT for upper extremity lymphedema from May 2017 to April 2022 was *** were divided into fat-or fluid-dominant phenotypes based on preoperative *** with a minimum of 12-month follow-up were *** were reviewed for demographic,intraoperative,and surveillance ***:Twenty-three patients underwent VLNT of the upper extremity during the study period,of which eighteen met the study *** patients had fluid-dominant disease and nine patients had fat-dominant disease and had undergone prior debulking at our ***-dominant patients demonstrated slight reductions in limb volume and hours in compression,and improvement in quality-of-life scores at twelve ***-dominant patients who underwent prior debulking had a slight increase in limb volume without a change in hours of compression,and demonstrated improvements in quality-of-life scores in nearly all ***,17% of patients discontinued compression therapy *** in extremity edema was present in 83% of postoperative ***:VLNT had varying effects on limb measurements while reliably improving quality-of-life and allowing for the potential of discontinuing *** a staged approach wherein debulking is performed upfront may be particularly beneficial for patients with fat-dominant disease.

关键词： Lymphedema vascularized lymph node transplant lymphatic surgery

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App-based assessment of memory functions in patients after transfemoral aortic valve replacement

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Journal of Geriatric Cardiology 2023年第9期20卷 664-672页

作者： jonathan Nübel Michael Hauptmann Julika Schön Georg Fritz Christian Butter Anja Haase-Fielitz Department of Cardiology University Hospital Heart Centre BrandenburgBrandenburg Medical School(MHB)Theodor FontaneBernauGermany Institute of Biostatistics and Registry Research Brandenburg Medical School Theodor FontaneNeuruppinGermany Faculty of Health Sciences(FGW) Joint Faculty of the University of Potsdamthe Brandenburg Medical School Theodor Fontane and the Brandenburg Technical University Cottbus-SenftenbergCottbusGermany Anesthesia and Intensive Care University Hospital Ruppin Brandenburg(ukrb)Brandenburg Medical School(MHB)Theodor FontaneNeuruppinGermany Department of Anesthesiology Intensive Care and Pain TherapyUniversity Hospital Heart Centre BrandenburgBrandenburg Medical School(MHB)Theodor FontaneBernauGermany Institute of Social Medicine and Health System Research Otto von Guericke University MagdeburgGermany

BACKGROUND Transfemoral aortic valve replacement(TAVR)is the standard treatment for elderly patients with aortic valve *** safe and well-established,there is a risk of intraprocedural hemodynamic instability and silent cerebral embolism,which can lead to a decline in neurocognitive function and *** clinical practice,comprehensive cognitive testing is difficult to ***-assisted digital applications may help to optimize diagnosis and *** Neurocognitive function was assessed by validated psychometric tests using“Δelta-App”,which uses artificial intelligence and computational linguistic methods for extraction and *** function was assessed using the‘Consortium to Establish a Registry for Alzheimer’s Disease’(CERAD)word list and digit span task(DST)before TAVR and before hospital *** study is registered in the German Register of Clinical Trials(https://***/search/de/trial/DRKS00020813).RESULTS From October 2020 until March 2022,141 patients were enrolled at University Hospital Heart Centre *** age was 81±6 years,42.6%were *** between the pre-and post-interventional test was on average 6±3 *** function before TAVR was found to be below average in relation to age and educational *** pre-post TAVR comparison showed significant improvements in the wordlist repeat,pppan *** Despite impaired preoperative memory function before TAVR,no global negative effect on memory function after TVAR was *** improvements shown in the word list test should be interpreted as usual learning effects in this task.

关键词： patients impaired valve

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Tunneled biopsy is an underutilised,simple,safe and efficient method for tissue acquisition from subepithelial tumours

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World Journal of Clinical Cases 2021年第21期9卷 5822-5829页

作者： Andreas Koutsoumpas Ruwan perera Adele Melton jonathan Kuker Tilak Ghosh Barbara Braden Translational Gastroenterology Unit Oxford University Hospitals NHS Foundation TrustOxford OX39DUUnited Kingdom Senior School Magdalen College SchoolOxford OX40DZUnited Kingdom Translational Gastroenterology Unit Oxford University Hospital NHS Foundation TrustOxford OX39DUUnited Kingdom

BACKGROUND Tissue acquisition from subepithelial lesions is often attempted by endoscopic ultrasound(EUS)-sampling as conventional endoscopic biopsy usually fails to reach deeper layers of the gastrointestinal *** To investigate the utilisation,safety and diagnostic yield of an intensified“biteon-bite”tunnel biopsy *** In this retrospective cohort study,all patients presenting with subepithelial masses in the upper gastrointestinal tract from March 2013 to July 2019 were *** were analysed for size and location of the subepithelial mass,use of intensified tunnel biopsy protocol(more than 10 double bite-on-bite biopsies)or superficial conventional biopsies,histology and imaging results,occurrence of readmission and adverse events after *** Two hundred and twenty-nine patients with subepithelial lesions were *** conventional biopsies were taken in 117 patients and were diagnostic only in one lipoma(0.9%).Tunnel biopsies taken in 112/229(48.9%)patients were significantly more likely to provide histological diagnosis(53.6%;ppsies further increased to 41/67(61.2%).No immediate or delayed complications were *** 8 of the 51 endoscopists(15.7%)regularly attempted tunnel *** Tunnel biopsy is a simple,safe and efficient but underutilised diagnostic modality for tissue acquisition in subepithelial *** should be routinely attempted at the initial endoscopy.

关键词： Gastrointestinal stromal tumour Fine needle biopsy Lipoma Immunohistology Neuroendocrine tumours Fine needle biopsy

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Multiscale patterning of a metallic glass using sacrificial imprint lithography

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Microsystems & Nanoengineering 2015年第1期1卷 16-25页

作者： jonathan p.Singer Candice I.pelligra Noga Kornblum Youngwoo Choo Manesh Gopinadhan punnathat Bordeenithikasem Jittisa Ketkaew Seng Fatt Liew Hui Cao Jan Schroers Chinedum O.Osuji Department of Mechanical and Aerospace Engineering Rutgers UniversityPiscatawayNJ 08854USA Department of Chemical and Environmental Engineering Yale UniversityNew HavenCT 06511USA Department of Chemical and Environmental Engineering Center for Research on Interfacial PhenomenaYale UniversityNew HavenCT 06511USA Department of Mechanical Engineering and Materials Science Yale UniversityNew HavenCT 06511USA Department of Applied physics Yale UniversityNew HavenCT 06511USA Department of Mechanical Engineering and Materials Science Center for Research on Interfacial PhenomenaYale UniversityNew HavenCT 06511USA

Bulk metallic glasses(BMGs)have been developed as a means to achieve durable multiscale,nanotextured surfaces with desirable properties dictated by topography for a multitude of *** barrier to this achievement is the lack of a bridging technique between macroscale thermoplastic forming and nanoimprint lithography,which arises from the difficulty and cost of generating controlled nanostructures on complex geometries using conventional top-down *** difficulty is compounded by the necessary destruction of any resulting reentrant structures during rigid *** have developed a generalized method to overcome this limitation by sacrificial template imprinting using zinc oxide(ZnO)*** is established that such structures can be grown inexpensively and quickly with tunable morphologies on a wide variety of substrates out of solution,which we exploit to generate the nanoscale portion of the multiscale pattern through this bottom-up *** this way,we achieve metallic structures that simultaneously demonstrate features from the macroscale down to the nanoscale,requiring only the top-down fabrication of macro/microstructured *** detachment of the formed part from the multiscale molds,the ZnO remains embedded in the surface and can be removed by etching in mild conditions to both regenerate the mold and render the surface of the BMGs *** ability to pattern metallic surfaces in a single step on length scales from centimeters down to nanometers is a critical step toward fabricating devices with complex shapes that rely on multiscale topography for their intended functions,such as biomedical and electrochemical applications.

关键词： bulk metallic glasses nanoimprint multiscale structures optical properties

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predisposing factors for positive D-Xylose breath test for evaluation of small intestinal bacterial overgrowth:A retrospective study of 932 patients

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World Journal of Gastroenterology 2015年第15期21卷 4574-4582页

作者： Richard A Schatz Qing Zhang Nilesh Lodhia jonathan Shuster phillip p Toskes Baharak Moshiree Department of Medicine Medical University of South Carolina Department of Gastroenterology University of Florida Gainesville Department of Gastroenterology Medical University of South Carolina Charleston Department of Health Outcomes and policy University of Florida Gainesville Department of Gastroenterology University of Miami

AIM: To investigate, in the largest cohort to date, patient characteristics and associated risk factors for developing small intestinal bacterial overgrowth(SIBO) using the D-Xylose breath test(XBT).METHODS: We performed a retrospective crosssectional study to analyze patient characteristics who underwent the XBT for evaluation of SIBO. Diagnostic testing with the XBT was performed based on a clinical suspicion for SIBO in patients with symptoms of bloating, abdominal pain, abdominal distension, weight loss, diarrhea, and/or constipation. Consecutive electronicmedical records of 932 patients who completed the XBT at the University of Florida between 2005 and 2009 were reviewed. A two-way Analysis of Variance(ANOVA) was used to test for several associations including age, gender, and body mass index(BMI) with a +XBT. A two-way ANOVA was also performed to control for the differences and interaction with age and between genders. A similar analysis was repeated for BMI. Associations between medical conditions and prior surgical histories were conducted using the Mantel-Haenszel method for 2 by 2 contingency tables, stratified for gender. Reported odds ratio estimates reflect the odds of the prevalence of a condition within the +XBT group to that of the-XBT group. p values of less than 0.05(two-sided) were considered statistically ***: In the 932 consecutive eligible subjects studied, 513 had a positive XBT. A positive association was found between female gender and a positive XBT(p = 0.0025), and females with a positive test were, on average, greater than 5 years older than those with a negative test(p = 0.024). The mean BMI of positive XBT subjects was normal(24.5) and significantly lower than the subjects with a negative XBT(29.5)(p = 0.0050). A positive XBT was associated with gastroesophageal reflux disease(GERD)(OR = 1.35; 95%CI: 1.02-1.80, p = 0.04), peptic ulcer disease(pUD)(OR = 2.61; 95%CI: 1.48-4.59, p paresis(Gp)(OR = 2.04; 95%C

关键词： Intestine Small Irritable bowel syndrome Xylose Breath tests proton pump inhibitors Gastroparesis Bacteria

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Occurrence,distribution and ecological risk assessment of organophosphorus flame retardants and plasticizers in sediment samples along the Vaal River catchment,South Africa

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Emerging Contaminants 2019年第1期5卷 173-178页

作者： Tlou B.Chokwe jonathan O.Okonkwo Scientific Services Rand WaterP.O.Box 3526Vereeniging1930South Africa Department of Water Environmental and Earth SciencesTshwane University of Technology175 Mandela RoadPretoria0001South Africa

The occurrence,distribution and ecological risk assessment of twelve(12)organophosphorus flame retardants(OpFRs)were investigated in sediments from the Vaal River,South *** halogenated OpFRs[tris-(2-chloroethyl)phosphate(TCEp),three isomers of tris-(chloropropyl)phosphate(TCipps),tris-(1,3-dichloro-2-propyl)phosphate(TDCipp)and tris-(2,3 dibromopropyl)phosphate(TDBpp)]and six non-halogenated OpFRs[tris-(n-butyl)phosphate(TBp),tris-(phenyl)phosphate(Tphp),tris-(butyl ethoxy)phosphate(TBOEp),tris-(2-ethylhexyl)phosphate(TEHp),diphenyl-2-ethylhexyl phosphate(DpEHp)and tris-(m-cresyl)phosphate(m-TCp)]were detected in this *** concentrations of twelve OpFRs in total(S12OpFRs)ranged from 68 to 278 ng g^-1 dry weight(dw)with the mean and median of 138 and 120 ng g^-1;*** non-halogenated OpFRs showed more abundances than the halogenated OpFRs with TDBpp,TBp,and TBOEp the most abundant ***,concentrations of OpFRs in more urbanized region were higher than those in less urbanized *** the concentrations of OpFRs and total organic carbon(TOC)were correlated,a moderate and significant correlation(pp poses high risk(HQ>1)while TBOEp,Tphp,and TBp posed medium risk(0.1posing negligible risk(HQprovides valuable information on the occurrence and risks of the investigated OpFRs within Vaal River.

关键词： OpFRs Concentrations Risk assessment Sediments Vaal river

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成纤维细胞生长因子1在促人脂肪细胞形成中抑制Wnt/β-catenin信号通路

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中南大学学报（医学版） 2015年第8期40卷 843-850页

作者：罗肖贾如李柯朱小瑛赵丹文 jonathan p.Whitehead 闫剑群西安交通大学医学部生理学与病理生理学系教育部环境与疾病相关基因重点实验室西安710061 昆士兰大学医学院mater医学研究中心肥胖与代谢疾病研究室

目的:研究成纤维细胞生长因子1(fibroblast growth factor-1,FGF-1)调控人脂肪细胞形成的时序性,明确其潜在的分子调控机制。方法:以过度生长综合征(Simpson-Golabi-Behmel syndrome,SGBS)患者前体脂肪细胞为模型,利用人重组蛋白FGF-1... 详细信息

目的:研究成纤维细胞生长因子1(fibroblast growth factor-1,FGF-1)调控人脂肪细胞形成的时序性,明确其潜在的分子调控机制。方法:以过度生长综合征(Simpson-Golabi-Behmel syndrome,SGBS)患者前体脂肪细胞为模型,利用人重组蛋白FGF-1处理细胞,分别于细胞增殖期(D-3)、融合期(D0)、诱导分化前期(D3)、中期(D7)及成熟期(D14)收集细胞,通过油红O染色及油红O提取法观察细胞内脂滴聚集情况,运用实时定量pCR及Western印迹方法分析成脂相关基因及Wnt/β-catenin信号通路相关因子的时序表达情况。结果:与对照组相比,FGF-1显著增加了SGBS细胞的脂质聚集,持续促进过氧化物酶增殖物激活受体(peroxisome proliferater-activated receptorγ,ppARγ)、甘油醛-3-磷酸脱氢酶(glyceraldehyde-3-phosphate dehydrogenase,G3pDH)、脂连蛋白(adiponectin)和葡萄糖转运体4(glucose transporter type 4,GLUT4)m RNA的高表达;同时,FGF-1显著下调Wnt/β-catenin信号通路相关因子β连环蛋白(β-catenin)和转录因子4(transcription factor 4,TCF4)的表达。结论:FGF-1促进SGBS脂肪形成的作用贯穿整个增殖及分化期,提示FGF-1促进脂肪形成的作用是通过调节Wnt/β-catenin信号通路来完成的。

关键词：成纤维细胞生长因子1 脂肪细胞分化 Wnt/β-catenin信号通路

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Validation and reproducibility of the Heidelberg Edge perimeter in the detection of glaucomatous visual field defects

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International Journal of Ophthalmology(English edition) 2019年第4期12卷 577-581页

作者： Qi N.Cui priyanka Gogt jonathan M.Lam Safa Siraj Lisa A.Hark jonathan S.Myers L.Jay Katz Michael Waisbourd Glaucoma Research Center Wills Eye Hospital Scheie Eye Center University of Pennsylvania Department of Ophthalmology Tel Aviv Sourasky Medical Center affiliated to the Sackler Faculty of Medicine Tel Aviv University

AIM: To validate the ability of the Heidelberg Edge perimeter(HEp) in detecting glaucomatous visual field(VF) defects compared to the Octopus Visual Field(OVF) Analyzer and to determine the test-retest repeatability of both ***: This prospective, cross-sectional study was conducted at Wills Eye Hospital, philadelphia, pA. Glaucoma subjects and unaffected controls underwent VF testing using HEp standard automated perimetry(SAp) Ⅲ 30-2 Adaptive Staircase Thresholding Algorithm(ASTA) FAST protocol and OVF G-TOp white-on-white strategy. Testing order was randomized. pearson's correlation coefficient was calculated for both mean deviation(MD) and pattern standard deviation/square root of loss of variance(pSD/s LV). Receiver operating characteristic(ROC) curves were used to determine the diagnostic abilities of both modalities. Glaucoma subjects returned for repeat testing and intraclass correlation coefficients(ICCs) were calculated to assess test-retest repeatability. RESULTS: Seventy-nine subjects with glaucoma and 36 unaffected controls were enrolled. HEp and OVF were significantly correlated for both MD(r=-0.84; ppSD/s LV(r=0.79; pparable between HEp and OVF for both MD(0.74 and 0.79, respectively; p=0.26) and pSD/sL V(0.74 and 0.82; p=0.08). ICC was high for both HEp (0.96 for MD, 0.95 for pSD/s LV) and OVF(0.82 and 0.88, respectively). Mean test duration(min) was significantly shorter for OVF(2.63) compared to HEp(5.15; pp and OVF show strong correlation for both MD and pSD/s LV, and have similar validity for detecting glaucoma. The test-retest repeatability is high for both HEp and OVF, however the average testing duration of HEp is significantly longer than that of OVF.

关键词： glaucoma Heidelberg Edge perimeter standard automated perimetry Octopus Visual Field Analyzer visual field

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Organic transistor platform with integrated microfluidics for in-line multi-parametric in vitro cell monitoring

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Microsystems & Nanoengineering 2017年第1期3卷 184-195页

作者： Vincenzo F.Curto Bastien Marchiori Adel Hama Anna-Maria pappa Magali p.Ferro Marcel Braendlein jonathan Rivnay Michel Fiocchi George G.Malliaras Marc Ramuz Róisín M.Owens Department of Bioelectronics Ecole Nationale Supérieure des MinesCMP-EMSEMOC880 Avenue de MimetGardanne 13541France Flexible Electronics Department Ecole Nationale Supérieure des Mines CMP-EMSEMOC880 Avenue de MimetGardanne 13541France Department of Biomedical Engineering Northwestern UniversityEvanstonIL 60208USA

Future drug discovery and toxicology testing could benefit significantly from more predictive and multi-parametric readouts from in vitro *** the recent advances in the field of microfluidics,and more recently organ-on-a-chip technology,there is still a high demand for real-time monitoring systems that can be readily embedded with *** addition,multi-parametric monitoring is essential to improve the predictive quality of the data used to inform clinical studies that *** we present a microfluidic platform integrated with in-line electronic sensors based on the organic electrochemical *** goals are twofold,first to generate a platform to host cells in a more physiologically relevant environment(using physiologically relevant fluid shear stress(FSS))and second to show efficient integration of multiple different methods for assessing cell morphology,differentiation,and *** include optical imaging,impedance monitoring,metabolite sensing,and a wound-healing *** illustrate the versatility of this multi-parametric monitoring in giving us increased confidence to validate the improved differentiation of cells toward a physiological profile under FSS,thus yielding more accurate data when used to assess the effect of drugs or ***,this platform will enable high-content screening for in vitro drug discovery and toxicology testing and bridges the existing gap in the integration of in-line sensors in microfluidic devices.

关键词： bioelectronics in-line sensors in vitro microfluidics

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Deficiency of pyruvate dehydrogenase kinase 4 sensitizes mouse liver to diethylnitrosamine and arsenic toxicity through inducing apoptosis

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Liver Research 2018年第2期2卷 100-107页

作者： jonathan Choiniere Matthew Junda Lin Li Wang Jianguo Wu Department of physiology and Neurobiology Institute for Systems GenomicsUniversity of ConnecticutStorrsCTUSA Veterans Affairs Connecticut Healthcare System West HavenCTUSA Department of Internal Medicine Section of Digestive DiseasesYale UniversityNew HavenCTUSA School of pharmaceutical Sciences Wenzhou Medical UniversityWenzhouZhejiangChina

Background and Aim:pyruvate dehydrogenase kinase 4(pDK4)is a metabolism switch that regulates glucose oxidation and the tricarboxylic acid cycle(TCA cycle)in the *** detoxifies xenobiotics and is constantly challenged by various *** study aims at understanding how the loss of the metabolism regulator pDK4 contributes to liver ***:Wild-type(WT)and pdk4 knockout(pdk4-/-)mice of different ages were examined for spontaneous hepatic *** or adult mice of two genotypes were insulted by diethylnitrosamine(DEN),arsenic,galactosamine(GalN)/lipopolysaccharide(LpS),anti-CD95(Jo2)antibody or carbon tetrachloride(CCl4).Liver injury was monitored by blood biochemistry *** was determined by terminal deoxynucleotidyl transferase dUTp nick end labeling(TUNEL)staining,poly(ADp-ribose)polymerase(pARp)cleavage,and caspase activity *** response was determined by nuclear factor(NF)-kB activation and the activation of NF-kB target *** hepatocytes were isolated and cell viability was evaluated by MTS ***:We showed that systematic pdk4-/-in mice resulted in age-dependent spontaneous hepatic ***4-deficiency increased the toxicity of DEN in juvenile mice,which correlated with a lethal consequence and massive hepatic ***,chronic arsenic administration induced more severe hepatic apoptosis in pdk4-/-mice compared to WT control *** aggravated hepatic NF-kB mediated-inflammatory response was observed in pdk4-/-mice *** vitro,pdk4-deficient primary hepatocytes were more vulnerable to DEN and arsenic challenges and displayed higher caspase activity than wild type ***,hepatic pDK4 mRNA level was remarkably reduced during acute liver failure induced by GalN/LpS or Jo2 *** diminished pDK4 expression was also observed in CCl4-induced acute liver ***:pDK4 may contribute to the protection from apoptotic injury in mouse liver.

关键词： Hepatocytes Cell death Liver injury pyruvate dehydrogenase kinase 4(pDK4)

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