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Nutritional stimulation by in-ovo feeding modulates cellular proliferation and differentiation in the small intestinal epithelium of chicks

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Animal Nutrition 2022年第1期8卷 91-101页

作者： Naama Reicher Tal Melkman-Zehavi jonathan dayan Eric AWong Zehava Uni Department of Animal Science The Robert H.SmithFaculty of AgricultureFood and EnvironmentThe Hebrew University of JerusalemRehovot 76100Israel Department of Animal and Poultry Sciences Virginia TechBlacksburgVA 24061USA

Nutritional stimulation of the developing small intestine of chick embryos can be conducted by in-ovo feeding(IOF).We hypothesized that IOF of glutamine and leucine can enhance small intestinal development by promoting proliferation and differentiation of multipotent small intestinal epithelial *** embryos(n=128)were subject to IOF of glutamine(IOF-Gln),leucine(IOF-Leu),NaCl(IOF-NaCl)or no injection(control)at embryonic d 17(E 17).Multipotent,progenitor and differentiated cells were located and quantified in the small intestinal epithelium between E 17 and d 7 after hatch(D 7)in all treatment groups by immunofluorescence of SRY-box transcription factor 9(Sox9)and proliferating cell nuclear antigen(PCNA),in-situ hybridization of leucine-rich repeat containing G-protein coupled receptor 5(Lgr5)and peptide transporter 1(PepT1)and histochemical goblet cell *** effects of IOF treatments at E 19(48 h post-IOF),in comparison to control embryos,were as follows:total cell counts increased by 40%,33%and 19%,and multipotent cell counts increased by 52%,50%and 38%,in IOF-Gln,IOF-Leu and IOF-NaCl embryos,*** IOF-Gln embryos exhibited a significance,36%increase in progenitor cell *** IOF treatments shifted Lgr5+ stem cell localizations to villus *** differentiated,PepT1+region of the villi was 1.9 and 1.3-fold longer in IOF-Gln and IOF-Leu embryos,respectively,while goblet cell densities decreased by 20%in IOF-Gln ***,crypt and villi epithelial cell counts were significantly higher IOF-Gln chicks,compared to control chicks(P<0.05).We conclude IOF of glutamine stimulates small intestinal maturation and functionality during the peri-hatch period by promoting multipotent cell proliferation and differentiation,resulting in enhanced compartmentalization of multipotent and differentiated cell niches and expansions of the absorptive surface area.

关键词： Chick Intestinal development Proliferation Differentiation Multipotent cells In-ovo feeding

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