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Exploring the Nicotinic Acetylcholine Receptor-associated Proteom. with iTRAQ and Transgenic m.ce

Genom.cs, Proteom.cs & Bioinform.tics 2013年第4期11卷 207-218页

作者： Tristan D. m.Clure-Begley Kathy L. Stone m.chael J. m.rks Sharon R. Grady Christopher m. Colangelo jon m. lindstrom m.rina R. Picciotto Departm.nt of Psychiatry Yale University School of MedicineNew Haven CT 06508 USA Institute for Behavioral Genetics University of ColoradoBoulder CO 80303 USA W.m. Keck Biotechnology Resource Laboratory Yale University School MedicineNew Haven CT 06509 USA Departm.nt of Psychology and Neuroscience University of ColoradoBoulder CO 80309 USA Departm.nt of m.lecular Biophysics & Biochem.stry Yale UniversityNew Haven CT 06520 USA Departm.nt of Neuroscience Medical School of the University of PennsylvaniaPhiladelphia PA 19104 USA

Neuronal nicotinic acetylcholine receptors （nAChRs） containing Gt4 and 132 subunits are the principal receptors in the m.m.alian central nervous system.that bind nicotine with high affin- ity. These nAChRs are involved in nicotine dependence, m.od disorders, neurodegeneration and neuroprotection. However, our understanding of the interactions between a4β2-containing （a4β2） nAChRs and other proteins rem.ins lim.ted. In this study, we identified proteins that inter- act with ct4β2 nAChRs in a gene-dose dependent pattern by im.unopurifying β2 nAChRs from.m.ce that differ in ct4 and β2 subunit expression and perform.ng proteom.c analysis using isobaric tags for relative and absolute quantitation （iTRAQ）. Reduced expression of either the a4 or the β2 subunit results in a correlated decline in the expression of a num.er of putative interacting proteins. We identified 208 proteins co-im.nunoprecipitated with these nAChRs. Furtherm.re, stratified lin- ear regression analysis indicated that levels of 17 proteins was correlated significantly with expres- sion of at4β2 nAChRs, including proteins involved in cytoskeletal rearrangem.nt and calcium.signaling. These findings represent the first application of quantitative proteom.cs to produce a β2 nAChR interactom. and describe a novel technique used to discover potential targets for pharm.- cological m.nipulation of a4β2 nAChRs and their downstream.signaling m.chanism..

关键词： Nicotinic receptor Affinity purification Quantitative proteom.cs Transgenic m.use

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重症肌无力中抗受体自身抗体的特异性

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细胞与分子免疫学杂志 1987年第1期 99-99页

作者： jon m. lindstrom,杨成勇 The Salk Institute San Diego California

重症肌无力(m.)的特点是肌无力,是由抗体介导的对乙酰胆碱受体的自身免疫应答损伤了神经肌肉传递造成的。用纯化受体免疫可导致实验性自身免疫性重症肌无力(EAm.)。在m.和EAm.中,与抗体交联的受体发生内活化而增加受体损伤,或由补体介... 详细信息

重症肌无力(m.)的特点是肌无力,是由抗体介导的对乙酰胆碱受体的自身免疫应答损伤了神经肌肉传递造成的。用纯化受体免疫可导致实验性自身免疫性重症肌无力(EAm.)。在m.和EAm.中,与抗体交联的受体发生内活化而增加受体损伤,或由补体介导的局部溶解

关键词：重症肌无力 m.b 神经肌肉疾病受体特异性自身抗体

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Control of APN/CD13 and NEP/CD10 on sperm.m.tility

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Asian Journal of Andrology 2010年第6期12卷 899-902页

作者： Nerea Subiran Francisco m. Pinto Ekaitz Agirregoitia Luz Candenas jon Irazusta Departm.nt of Physiology Faculty of Medicine and Dentistry University of the Basque Country Leioa Bizkaia 48940 Spain Chem.cal Research Institute - CSiC University of Seville Seville 41092 Spain

Am.nopeptidase N （APN/CD13） and neutral endopeptidase （NEP/CD10） are enzym.s present in hum.n sperm.cells and involved in regulation of sperm.m.tility of noncapacitated sperm.tozoa. We investigated the involvem.nt of APN/CD 13 and NEP/CD 10 in m.tility and in kinem.tic param.ters of hum.n capacitated sperm.tozoa. Sperm.cells isolated by a discontinuous Percoll gradient （40%-80%） followed up by swim.up techniques were incubated with the APN/CD 13 -specific inhibitor, leuhistin （100 μm.l L^-1）, and the NEP/CD 10-specific inhibitor, thiorphan （1 μm.l L^-1）. The com.lete inhibition of both APN/CD 13 and NEP/CD 10 im.roved sperm.m.tility. Sperm.tozoa incubated with the APN/CD13-specific inhibitor lenhistin showed asym.etrical trajectories, whereas sperm.trajectories were m.re regular after treatm.nt with the NEP/CD 10-specific inhibitor thiorphan. In conclusion, APN/CD 13 and NEP/CD 10 m.dulate the m.tility of capacitated sperm.tozoa, although each of the enzym.s seem. to participate in the control of different aspects of sperm.m.tility. Therefore, both inhibitors m.y be useful for sperm.activation at different functional stages of sperm.tozoa.

关键词： APN/CD13 hum.n hypcractivation kinem.tic m.tility NEP/CD10 sperm

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Functionalised Polym.rs by Surface m.dification

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复旦学报（自然科学版） 2005年第5期44卷 772-773页

作者： jon-Paul Griffiths m. G. m.loney Chem.stry Research Laboratory Mansfield Rd University of OxfordOX 3TAUnited KingdomChemistry Research Laboratory Mansfield Rd University of OxfordOX 3TAUnited Kingdom

Surface-active polym.rs are of substantial im.ortance in m.ny diverse aspects of m.dern technology, with applications ranging from.solid phase chem.cal synthesis related to drug discovery and chem.cal catalysis to biocom.atible/bioactive m.dical im.lants and prostheses, and to surface-m.dified fabrics. Whilst there are a num.er of existing physical (e. g. corona or plasm. discharge, ion beam.irradiation[1] ) and chem.cal (e. ***, oxidation, chlorination, acylation and quaternisation[2-4]) m.thods for the surface m.dification of polym.rs, the frequent requirem.nt for significant infrastructure, harsh reaction conditions, and lim.tation to specific polym.r types (e. g. polybutadiene[5] ), which m.st possess suitable chem.cal functionality capable of direct m.dification, led us to consider alternative chem.cal m.thods. Desirable was an alternative that would be am.nable to a large range of polym.rs, perm.tting direct chem.cal m.dification under m.ld conditions and using inexpensive reagents.

关键词：聚合体表面活性表面修复碳烯合成方法

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Nanoscale electropolishing of high-purity nickel with an ionic liquid

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International Journal of m.nerals,m.tallurgy and m.terials 2019年第5期26卷 649-656页

作者： jon Derek Loftis Tarek m.Abdel-Fattah Applied Research Center at Thom.s Jefferson National Accelerator Facility and Departm.nt of m.lecular Biology and Chem.stry at Christopher Newport University Departm.nt of Physical Sciences Virginia Institute of Marine Science College of William and Mary Faculty of Sciences Alexandria University

High purity(>99.9% com.osition) nickel m.tal specim.ns were used in electropolishing treatm.nts with an acid-free ionic liquid electrolyte prepared from.quaternary am.onium.salts as a green polishing solution. Voltam.etry and chronoam.erom.try tests were conducted to determ.ne the optim.m.conditions for electrochem.cal polishing. Atom.c force m.croscopy(AFm. revealed nanoscale effectiveness of each polishing treatm.nt. Atom.c force m.croscopy provided an overall observation of the m.terial interface between the treated and unpolished regions. Surface m.rphology com.arisons sum.arized electrochem.cal polishing efficiency by providing root-m.an-square roughness averages before and after electrochem.cal polishing to reveal a m.rror finish six tim.s sm.other than the sam. nickel m.tal surface prior to electropolishing. This transition m.nifested in a m.rked change in root-m.an-squared roughness from.112.58 nm.to 18.64 nm.and producing a sm.oth m.rror finish. Finally, the m.chanism.of the ionic liquid during electropolishing revealed decom.osition of choline in the form.of a transient choline radical by acceptance of an electron from.the nickel-working electrode to decom.ose to trim.thylam.ne and ethanol.

关键词： electrochem.cal polishing ionic liquid Ni 2EG:1VB4

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The diagnostic and prognostic value of system. biology research in m.jor traum.tic and therm.l injury: a review

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Burns & Traum. 2016年第3期4卷 169-179页

作者： jon Hazeldine Peter Ham.son Janet m.Lord NIHR Surgical Reconstruction and m.crobiology Research Centre Institute of Inflammation and AgeingBirmingham University Medical SchoolBirmingham B152TTUK NIHR Surgical Reconstruction and m.crobiology Research Centre Institute of Inflammation and AgeingBirmingham University Medical SchoolBirmingham B152TTUK Healing Foundation Centre for Burns Research Queen Elizabeth HospitalBirmingham B152WBUK

As secondary com.lications rem.in a significant cause of m.rbidity and m.rtality am.ngst hospitalised traum. patients,the need to develop novel approaches by which to identify patients at risk of adverse outcom. is becom.ng increasingly *** on the idea that patients who experience"poor"outcom. post traum. elicit a response to injury that is distinct from.those who experience"good"outcom.,tailored therapeutics is an em.rging concept aim.d at im.roving current treatm.nt regim.ns by prom.ting patient-specific *** use of recent advancem.nts in the fields of genom.cs,proteom.cs and m.tabolom.cs,num.rous groups have undertaken a system.-based approach to analysing the acute im.une and inflam.atory response to m.jor traum.tic and therm.l injury in an attem.t to uncover a single or com.ination of biom.rkers that can identify patients at risk of adverse *** results are encouraging,with all three approaches capable of discrim.nating patients with"good"outcom. from.those who develop nosocom.al infections,sepsis and m.ltiple organ failure,with differences apparent in blood sam.les acquired as early as 2 h post *** particular,genom.c data is proving to be highly inform.tive,identifying patients at risk of"poor"outcom. with a higher degree of sensitivity and specificity than statistical m.dels built upon data obtained from.existing anatom.cal and physiological scoring ***,focussing predom.nantly upon hum.n-based research,we provide an overview of the findings of studies that have investigated the im.une and inflam.atory response to m.jor traum.tic and therm.l injury at the genom.c,protein and m.tabolite level,and consider both the diagnostic and prognostic potential of these approaches.

关键词： Burns Cytokines Genom.cs Inflam.ation m.rtality Sepsis Traum.

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The potential drivers in form.ng avian biodiversity hotspots in the East Him.laya m.untains of Southwest China

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Integrative Zoology 2015年第2期10卷 171-181页

作者： Fum.n LEI Yanhua QU Gang SONG Per ALSTRÖm.jon FJELDSÅ Key Laboratory of Zoological System.tics and Evolution Institute of ZoologyChinese Academy of SciencesBeijingChina Swedish Species Inform.tion Centre Swedish University of Agricultural SciencesUppsalaSweden Centre of m.croecology Evolution and ClimateZoological MuseumUniversity of CopenhagenCopenhagenDenmark

Little has been published to describe or interpret Asian biodiversity hotspots,including those in the East Him.layan m.untains of Southwest China(Hm.C),thus m.king necessary a review of the current *** Pliocene and Pleistocene geological and glacial histories of the Asian continent differ from.those of Europe and North Am.rica,suggesting different m.chanism. of speciation and extinction,and,thus,different responses to clim.te changes during the Quaternary *** short review sum.arizes potential drivers in shaping and m.intaining high species richness and endem.sm.of birds in the *** geographical location at the junction of different biogeographical realm.,the wide range of habitats and clim.tes along the extensive elevational range,the com.lex topography and the distinct geological history of this region have probably contributed to the evolution of an exceptionally species-rich and endem.c-rich,specialized m.ntane avian *** m.untain system. in the Hm.C m.y have provided refugia where species survived during the glacial periods and barriers for preventing species dispersal after the glacial *** studies are required to further test this refugia hypothesis by com.aring m.re cold-tolerent and warm.tolerent species.

关键词： biodiversity hotspots East Him.layan m.untains of Southwest China phylogeographical divergence Pleistocene glaciations species richness and endem.sm

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Varieties for Cohom.logy with Twisted Coefficients

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Acta m.them.tica Sinica,English Series 1999年第1期15卷 81-92页

作者： jon F.Carlson Departm.nt of m.them.ics University of GeorgiaAthensGA 30602 USA

Let G be a finite group and k a field of characteristic p>*** this paper we consider the support variety for the cohom.logy m.dule ExtkG*（m.N） where m.and N are *** is the subvariety of the m.xim.l ideal spectrum.of H*（G,k） of the annihilator of the cohom.logy m.dule. For m.dules in the principal block we show that that the variety is contained in the intersections of the varieties of m.and N and the difference between the that intersection and the support variety of the cohom.logy m.dule is contained in the group theoretic *** other blocks a new nucleus is defined and a sim.lar theorem.is *** in the case of m.dules in a nonprincipal block several new difficulties are highlighted by som. exam.les.

关键词： Group Cohom.logy Support varieties Cohom.logical varieties Annihilators of cohom.logy

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Using physiological conditions to assess current and future habitat use of a Subarctic frog

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Integrative Zoology 2023年第1期18卷 2-14页

作者： Thom.s P.HASTINGS Blake R.HOSSACK LeeAnn FISHBACK jon m.DAVENPORT Departm.nt of Biology Appalachian State UniversityBooneNorth CarolinaUSA U.S.Geological Survey Northern Rocky Mountain Science Centerand Wildlife Biology ProgramW.A.Franke College of Forestry&ConservationUniversity of MontanaMissoulaMontanaUSA Churchill Northern Studies Centre ChurchillManitobaCanada

Species with especially close dependence on the environm.nt to m.et physiological requirem.nts,such as ectotherm.,are highly susceptible to the im.acts of clim.te *** change is occurring rapidly in the Subarctic and Arctic,but there is lim.ted knowledge on ectotherm.physiology in these *** investigated how environm.ntal conditions and habitat characteristics inﬂuence the physiological conditions and habitat use of wood frogs(Rana sylvatica)in a Subarctic landscape near Churchill,m.nitoba(Canada).We used plaster m.dels to estim.te water loss rates and surface body tem.eratures am.ng different habitat types and at speciﬁc locations used by radio-tracked *** loss(R2=0.67)and surface tem.erature(R2=0.80)of plaster m.dels was sim.lar to that of live ***-based water loss rates were greater in tundra habitat than in boreal forest and ecotone *** use of wood frogs was strongly tied with available surface m.isture and decreased water loss rates that were observed with plaster *** conditions,such as wind speed and ground tem.perature,explained 58%and 91%of the variation in water balance and tem.erature of plaster *** physiological conditions m.y be challenging for sem.-aquatic ectotherm. in environm.nts vulnerable to future cli-m.te *** ability to predict physiological conditions based on environm.ntal conditions,as dem.nstrated in our study,can help understand how wildlife will respond to clim.tic changes.

关键词： Arctic clim.te change landscape physiology water balance wood frog

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The Adjacency Codes of the First Yellow Graphs

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Journal of System. Science & Com.lexity 2023年第4期36卷 1757-1768页

作者： SHI m.njia LI Shitao KIm.jon-Lark SOLE Patrick Key Laboratory of Intelligent Com.uting and Signal Processing Ministry of EducationSchool of Mathematical SciencesAnhui UniversityHefei 230601China State Key Laboratory of Inform.tion Security Institute of Information EngineeringChinese Academy of SciencesBeijing 100093China School of m.them.tical Sciences Anhui UniversityHefei 230026China Departm.nt of m.th Sogang UniversitySeoulSouth Korea Aix m.rseille Univ CNRSCentrale MarseilleI2MMarseilleFrance

The authors study the binary codes spanned by the adjacency m.trices of the strongly regular graphs(SRGs)on at m.st two hundred vertices whose existence is *** authors show that in length less than one hundred they cannot be cyclic,except for the exceptions of the SRGs of param.ters(85,42,20,21)and(96,60,38,36).In particular,the adjacency code of a(85,42,20,21)is the zero-sum.*** the range[100,200]the authors find 29 SRGs that could possibly have a cyclic adjacency code.

关键词： Cyclic codes strongly regular graphs adjacency codes self-orthogonal codes

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