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A Comparative Study of Soluble Protein Extractions of Populus deltoides × （ Trichocarpa × Deltoides） for 2-DE

Journal of Life Sciences 2012年第9期6卷 970-979页

作者： joke dupae Ann Cuypers Jean-Paul Noben Jana Boulet Nele Weyens Karen Verstraelen Jaco Vangronsveld Centre for Environmental Sciences Hasselt University Agoralaan Building D B-3590 Diepenbeek Belgium Biomedical Institute Hasselt University Agoralaan Building A 3590 Diepenbeek Belgium

Background: The disclosure of the poplar genome strengthens its position as well-established model organism. Populus has been subject of several proteome studies, but up to date no comparative study was performed on the extraction method of soluble proteins for this species. The extraction is the most critical step in two-dimensional gel electrophoresis and each extraction method has its advantages, disadvantages and limitations. Therefore protein extraction methods should be optimized for each tissue before starting an experimental setup. In prospect of future DIGE (Differential Gel electrophoresis) experiments for the investigation of the effects of cadmium and inoculation with plant growth promoting bacteria at the proteome level, the aim of this study was to optimize an extraction method for soluble proteins of poplar leaves and roots. Results: The acetone-phenol extraction method was found to be the most suited, rendering a high spot number and low background interference. During further optimization, several critical steps in the extraction method were revealed. Conclusion: Aiming to optimize the extraction of soluble leaf and root proteins of Populus deltoides × (trichocarpa × deltoides) compatible with DIGE analysis, a protocol rendering high reproducibility, low background interference and a high spot number was established, however no novel insights were acquired.

关键词：可溶性蛋白美洲黑杨杨树叶提取物 2-DE 二维凝胶电泳提取方法蛋白质组

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