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HBV cccDNA in patients' sera as an indicator for HBV reactivation and an early signal of liver damage

World Journal of Gastroenterology 2004年第1期10卷 82-85页

作者： johnny sze The Institute of Molecular Biology The University of Hong KongHong KongChina

AIM:To evaluate the covalently closed circle DNA (cccDNA) level of hepatitis B virus (HBV) in patients' liver and sera. METHODS:HBV DNA was isolated from patients' liver biopsies and sera.A sensitive real-time PCR method,which is capable of differentiation of HBV viral genomic DNA and cccDNA,was used to quantify the total HBV *** total HBV viral DNA was quantitated by real-time PCR using a HBV diagnostic kit (PG Biotech,LTD,Shenzhen,China) described previously. RESULTS:For the first time,we measured the level of HBV DNA and cccDNA isolated from ten HBV patients' liver biopsies and *** the liver biopsies,cccDNA was detected from all the biopsy *** copy number of cccDNA ranged from from 0.03 to 173.1 per cell,the copy number of total HBV DNA ranged from 0.08 to 3 717 per *** ratio of total HBV DNA to cccDNA ranged from 1 to 3 *** the sera, cccDNA was only detected from six samples whereas HBV viral DNA was detected from all ten *** ratio of cccDNA to total HBV DNA ranged from 0 to 1.77%.To further investigate the reason why cccDNA could only be detected in some patients' sera,we performed longitudinal *** cccDNA was detected from the patients' sera with HBV reactivation but not from the patients' sera without HBV *** level of cccDNA in the sera was correlated with ALT and viral load in the HBV reactivation patients. CONCLUSION:HBV cccDNA is actively transcribed and replicated in some patients' hepatoo/tes,which is reflected by a high ratio of HBV total DNA vs *** of cccDNA in the liver biopsy will provide an end-point for the anti-HBV *** occurrence of cccDNA in the sera is an early signal of liver damage,which may be another important clinical parameter.

关键词： Alanine Transaminase Biopsy DNA, Circular DNA, Viral Hepatitis B virus Hepatitis B, Chronic Hepatocytes Humans Kinetics Liver Research Support, Non-U.S. Gov't Viral Load

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Detection of CCRK mutation and transcriptional regulation in human glioblastoma

Detection of CCRK mutation and transcriptional regulation in...

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中国抗癌协会神经肿瘤专业委员会第八届学术会议

作者： Jamie Y.Chu johnny sze Julia J.Li Wai S.Poon Marie C.Lin Brain Tumour Centre Division of NeurosurgeryDepartment of SurgeryPrince of Wales HospitalThe Chinese University of Hong Kong

＜正＞Glioblastoma（GBM） is a multi-step process,including accumulation of genomic alterations and oncogene activation,which contributes to failure of current therapy. Therefore,discovery of new gene-targets for effi...

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Novel N-heteroaromatic dyes for textile substrates

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Chinese Chemical Letters 2014年第8期25卷 1165-1168页

作者： Kim-Hung Lam Sarah sze-Wah Ho Pik-Ling Lam johnny Cheuk-On Tang Zhao-Xiang Bian Albert Sun-Chi Chan Hua Li Wai-Yeung Wong Chung-Hin Chui Department of Applied Biology and Chemical Technology Institute of Textile and Clothing The Hong Kong Polytechnic University Clinical Division School of Chinese Medicine Hong Kong Baptist University Institute of Molecular Functional Materials Department of Chemistry and Institute of Advanced Materials Hong Kong Baptist University College of Life and Environmental Sciences Minzu University of China HKBU Institute of Research and Continuing Education Shenzhen Virtual University Park

This study reports the synthesis and characterization of quinoline derivatives. The synthesized quinoline compounds were applied to the dyeing treatment of acrylic fabrics. The color fastness to washing and light of quinoline compound-dyed fabrics was examined. Human skin HaCaT cell line was also used for the skin cytotoxicity evaluation of the quinoline compound-dyed acrylic fabrics.

关键词： Acrylic fabrics Fluorescent Quinoline dyes Skin cytotoxicity

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Two Cancers Arising from Different Mesenchymal Origins: An Unexpected Connection over Time and Space

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Case Reports in Clinical Medicine 2019年第5期8卷 99-104页

作者： Natascha Putri sze Min Lek Nicholas Brian Shannon Joey Wee-Shan Tan Grace Hwei Ching Tan Claramae Shulyn Chia Melissa Ching Ching Teo Choon Hua Thng Timothy Kwang Yong Tay Chin-Ann johnny Ong Tina Puay Theng Koh Division of Surgical Oncology National Cancer Centre Singapore Singapore City Singapore Division of Oncologic Imaging National Cancer Centre Singapore Singapore City Singapore Department of Anatomical Pathology Singapore General Hospital Singapore City Singapore

Sarcomas are rare tumours, accounting for 1% of all malignancies. They are histologically diverse, presenting with more than 50 different subtypes. Sarcoma can be broadly divided into two categories—soft tissue sarcoma and bone sarcoma. Extraskeletal osteosarcoma is a unique subtype which is histologically similar to bone osteosarcoma but resides within soft tissue and has no attachments to bone. We present a rare case of a patient who initially presented with an extraskeletal osteosarcoma that was complicated by a chronic chest wall sinus and lymphedema of the left arm. He later developed enlarging left chest wall nodules at the same region that were proven to be dedifferentiated liposarcoma on biopsy 20 years later. We examine the occurrence of both extremely rare diseases of differing pathologies over the same site but separated by an extraordinarily long interval, and possible new associations to account for this phenomenon.

关键词： Sarcoma Extraskeletal Osteosarcoma Dedifferentiated Liposarcoma Osteogenic Sarcoma

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