检索结果-南通市图书馆

Nucleotide sequence conservation of novel and established cis-regulatory sites within the tyrosine hydroxylase gene promoter

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Frontiers in Biology 2015年第1期10卷 74-90页

作者： Meng wANG Kasturi BANERJEE Harriet BAKER john w. CAVE Burke Medical Research Institute White Plains NY 10605 USA weill Cornell Medical College Brain and Mind Research Institute NY 10065 USA

Tyrosine hydroxylase （TH） is the rate-limiting enzyme in catecholamine biosynthesis and its gene proximal promoter （〈 1 kb upstream from the transcription start site） is essential for regulating transcription in both the developing and adult nervous systems. Several putative regulatory elements within the TH proximal promoter have been reported, but evolutionary conservation of these dements has not been thoroughly investigated. Since many vertebrate species are used to model development, function and disorders of human catecholaminergic neurons, identifying evolutionarily conserved transcription regulatory mechanisms is a high priority. In this study, we align TH proximal promoter nucleotide sequences from several vertebrate species to identify evolutionarUy conserved motifs. This analysis identified three elements （a TATA box, cyclic AMP response element （CRE） and a 5＇-GGTGG-3＇ site） that constitute the core of an ancient vertebrate TH promoter. Focusing on only eutherian mammals, two regions of high conservation within the proximal promoter were identified： a -250 bp region adjacent to the transcription start site and a -85 bp region located approximately 350 bp further upstream. within both regions, conservation of previously reported cis-regulatory motifs and human single nucleotide variants was evaluated. Transcription reporter assays in a TH-expressing cell line demonstrated the functionality of highly conserved motifs in the proximal promoter regions and electromobility shift assays showed that brain-region specific complexes assemble on these motifs. These studies also identified a non-canonical CRE binding （CREB） protein recognition element in the proximal promoter. Together, these studies provide a detailed analysis of evolutionary conservation within the TH promoter and identify potential cisregulatory motifs that underlie a core set of regulatory mechanisms in mammals.

关键词： catecholamine dopamine transcription evolution

pH modeling for maximum dissolved organic matter removal by enhanced coagulation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2012年第2期24卷 276-283页

作者： Jiankun Xie Dongsheng wang john van Leeuwen Yanmei Zhao Linan Xing Christopher w. K. Chow State Key Laboratory of Environmental Aquatic Chemistry Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China SA water Centre for water Management and Reuse NBE Universit of South Australia Australia Australian water Quality Centre SA Water 250 Victoria Square Adelaide SA 5000 Australia

Correlations between raw water characteristics and pH after enhanced coagulation to maximize dissolved organic matter（DOM） removal using four typical coagulants（FeCl3,Al2（SO4）3,polyaluminum chloride（PACl） and high performance polyaluminum chloride（HPAC）） without pH control were *** correlations were analyzed on the basis of the raw water quality and the chemical and physical fractionations of DOM of thirteen Chinese source waters over three *** was found that the final pH after enhanced coagulation for each of the four coagulants was in？uenced by the content of removable DOM（*** and higher apparent molecular weight（AMw） DOM）,the alkalinity and the initial pH of raw water.A set of feed-forward semi-empirical models relating the final pH after enhanced coagulation for each of the four coagulants with the raw water characteristics were developed and optimized based on correlation *** established models were preliminarily validated for prediction purposes,and it was found that the deviation between the predicted data and actual data was *** result demonstrated the potential for the application of these models in practical operation of drinking water treatment plants.

关键词： enhanced coagulation modeling DOM fractionation pH control

Fungal-derived selenium nanoparticles and their potential applications in electroless silver coatings for preventing pin-tract infections

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Regenerative Biomaterials 2022年第1期9卷 41-51页

作者： Xinjin Liang Shuai Zhang Geoffrey Michael Gadd john McGrath David w.Rooney Qi Zhao The Bryden Centre School of Chemical and Chemistry EngineeringQueen’s University BelfastUniveristy RoadBelfast BT71NNUK Geomicrobiology Group School of Life SciencesUniversity of DundeeDow StreetDundee DD15EHUK School of Pharmacy Queen’s University BelfastLisburn RoadBelfast BT97BLUK State Key Laboratory of Heavy Oil Processing Beijing Key Laboratory of Oil and Gas Pollution ControlCollege of Chemical Engineering and EnvironmentChina University of Petroleum18 Fuxue RoadChangping DistrictBeijing 102249China School of Biological Sciences Queen’s University BelfastChlorine GardensBelfast BT95DLUK School of Chemistry and Chemical Engineering Queen’s University BelfastStranmillis RoadBelfast BT95AGUK School of Science and Engineering University of DundeeNethergateDundee DD14HNUK

Pin-tract infections(PTIs)are a common complication of external fixation of fractures and current strategies for preventing PTIs have proven to be *** advances show that the use of anti-infection coatings with local antibacterial activity may solve this *** has been considered as a promising anti-infection agent owing to its antibacterial and antibiofilm *** this study,selenium nanoparticles(SeNPs)were synthesized via a cost-effective fungi-mediated biorecovery approach and demonstrated excellent stability and *** investigate their anti-infection potential,the SeNPs were doped in silver coatings through an electroless plating process and the silver–selenium(Ag–Se)coatings were tested for antibacterial and antibiofilm properties against Staphylococcus aureus F1557 and Escherichia coli wT F1693 as well as corrosion resistance in simulated body *** was found that the Ag–Se coating significantly inhibited *** growth and biofilm formation on the surface,reducing 81.2%and 59.7%of viable bacterial adhesion when compared with Ag and Ag–PTFE-coated surfaces after 3 *** Ag–Se coating also exhibited improved corrosion resistance compared with the Ag coating,leading to a controlled release of Ag^(+),which in turn reduced the risk of cytotoxicity against *** results suggest that the fungal-derived SeNPs may have potential in use as implant coatings to prevent PTIs.

关键词： infection coating silver selenium fungi

Orchard Sports Injury and Illness Classification System (OSIICS) Version 15

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Sport and Health Science 2024年第4期13卷 599-604页

作者： john w.Orchard Ebonie Rio Kay M.Crossley Jessica J.Orchard Margo Mountjoy School of Public Health University of SydneySydneyNSW 2006Australia La Trobe Sport and Exercise Medicine Research Centre La Trobe UniversityMelbourneVIC 3083Australia Department of Family Medicine McMaster UniversityHamilton L8S 4L8Canada

Background:Sports medicine(injury and illnesses)requires distinct coding systems because the International Classification of Diseases is insuf-ficient for sports medicine *** Orchard Sports Injury and Illness Classification System(OSIICS)is one of two sports medicine coding systems recommended by the International Olympic *** updates of coding systems are ***:For Version 15,updates for mental health conditions in athletes,sports cardiology,concussion sub-types,infectious diseases,and skin and eye conditions were considered particularly ***:Recommended codes were added from a recent International Olympic Committee consensus statement on mental health conditions in *** landmark sports cardiology papers were used to update a more comprehensive list of sports cardiology *** union protocols on head injury assessment were used to create additional concussion ***:It is planned that OSIICS Version 15 will be translated into multiple new languages in a timely fashion to facilitate international *** large number of recently published sport-specific and discipline-specific consensus statements on athlete surveillance warrant regular updating of OSIICS.

关键词： Sports cardiology Dermatology Eye injuries Concussion Infectious diseases Sports injury classification

Life-span development / 9th ed.

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Life-span development /

Suppressor of Runnerless Encodes a DELLA Protein that Controls Runner Formation for A=sexual Reproduction in Strawberry

2004年

作者： Santrock john w.

来源：畅想之星图书光盘版（南... 评论

在线全文

畅想之星图书光盘版（南通市图书馆镜像）

学校读者我要写书评

暂无评论

Molecular Plant 2018年第1期11卷 230-233页

作者： Julie C. Caruana john w. Sittmann wanpeng wang Zhongchi Liu Department of Cell Biology and Molecular Genetics University of Maryland College Park MD 20742 USA

Dear Editor, Strawberry is an economically important fruit crop contributing to human nutrition and agricultural output. The commercial success of this fruit crop is due in part to its asexual propagation through runn...

关键词：

An infertile male with dilated seminal vesicles due to functional obstruction

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2017年第2期19卷 256-257页

作者： Michael D Font Alexander w Pastuszak john R Case Larry I Lipshult Baylor College of Medicine Houston TX 77030 USA Center for Reproductive Medicine Baylor College of Medicine Houston TX 77030 USA Scott Department of Urology Baylor College of Medicine Houston TX 77030 USA Urology San Antonio San Antonio TX 78229 USA

Dear Editor, The patient is a 45-year-old male who presented to an outside clinic in 2014 for evaluation of painful ejaculation, infertility, and difficulty with urination for as long as he could remember. He reported no past significant medical or surgical history and no family history of infertility. He was given a trial of tamsulosin without improvement in his symptoms. Initial semen analysis demonstrated azoospermia.

关键词： infertile male dilated seminal vesicles due functional obstruction

Desialylated Platelet Clearance in the Liver is a Novel Mechanism of Systemic Immunosuppression

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Research 2024年第3期2023卷 35-50页

作者： June Li Danielle Karakas Feng Xue Yingyu Chen Guangheng Zhu Yeni H.Yucel Sonya A.MacParland Haibo Zhang john w.Semple john Freedman Qizhen Shi Heyu Ni Department of Laboratory Medicine and Pathobiology University of TorontoTorontoONCanada Toronto Platelet Immunobiology Group TorontoONCanada Keenan Research Centre for Biomedical Science of St.Michael's Hospital TorontoONCanada Canadian Blood Services Centre for Innovation TorontoONCanada Departments of Pediatrics Medical College of WisconsinMilwaukeeWIUSA Blood Research Institute Versiti WisconsinMilwaukeeWIUSA Departments of Ophthalmology and Vision Sciences Medicine University of TorontoTorontoONCanada Faculty of Engineering and Architectural Science Ryerson UniversityTorontoONCanada Multi-Organ Transplant Program Toronto General Hospital Research InstituteTorontoONCanada Department of Immunology University of TorontoTorontoONCanada Critical Care Medicine Department ofAnesthesiology and PainUniversity of TorontoTorontoONCanada Department of Physiology University of TorontoTorontoONCanada Department of Pharmacology University of TorontoTorontoONCanada. Division of Hematology and Transfusion Medicine Lund UniversityLundSweden clinical Immunology and Transfusion Medicine Ofice of Medical ServicesRegion SkaneLundSweden Department of Medicine University of TorontoTorontoONCanada Children's Research Institute Children's WisconsinWauwatosaWIUSA Midwest Athletes Against Childhood Cancer Fund Research Center MilwaukeeWIUSA.

Platelets are small, versatile blood cells that are critical for hemostasis/thrombosis. Local platelet accumulation is a known contributor to proinflammation in various disease states. However, the anti-inflammatory/immunosuppressive potential of platelets has been poorly explored. Here, we uncovered, unexpectedly, desialylated platelets (dPLTs) down-regulated immune responses against both platelet-associated and -independent antigen challenges. Utilizing multispectral photoacoustic tomography, we tracked dPLT trafficking to gut vasculature and an exclusive Kupffer cell-mediated dPLT clearance in the liver, a process that we identified to be synergistically dependent on platelet glycoprotein Ibα and hepatic Ashwell–Morell receptor. Mechanistically, Kupffer cell clearance of dPLT potentiated a systemic immunosuppressive state with increased anti-inflammatory cytokines and circulating CD4+ regulatory T cells, abolishable by Kupffer cell depletion. Last, in a clinically relevant model of hemophilia A, presensitization with dPLT attenuated anti-factor VIII antibody production after factor VIII ( infusion. As platelet desialylation commonly occurs in daily-aged and activated platelets, these findings open new avenues toward understanding immune homeostasis and potentiate the therapeutic potential of dPLT and engineered dPLT transfusions in controlling autoimmune and alloimmune diseases.

关键词： Platelet transfusion homeostasis

Reticulate spore ornamentation in Strobilomyces

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

菌物学报 2011年第6期30卷 901-911页

作者： Ronald H.Petersen john Dunlap Karen w.Hughes Department of Ecology and Evolutionary Biology University of TennesseeKnoxvilleTN 37996-1100USA Electron Microscope Facility Division of BiologyUniversity of TennesseeKnoxvilleTN 37996-0830USA

Reticulate spore ornamentation in Strobilomyces(Boletaceae,Basidiomycotina) is visible under light microscopy(bright field and phase contrast) up to 1,500×.while some distinctions can be made at this magnification,ontogeny and fine structure of the ornamentation cannot be *** electron microscope images,conversely,reveal significant additional structure from which the ontogenetic process can be *** numerous New and Old world collections,this paper presents evidence distinguishing reticulate ornamentation ontogeny in these disjunct populations.

关键词：网状孢子电子显微镜观察菌物学