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人类输卵管细胞来源的胚胎滋养因子-3(ETF-3)的判定

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人类输卵管细胞来源的胚胎滋养因子-3(ETF-3)的判定

Hepatic tight junctions:From viral entry to cancer metastasis

澳门、香港、内地生殖健康研讨会

作者： Yin—Lau Lee Kai—Fai Lee Jen—Fu Chiu Will m. Lee john m.luk William S.B.Yeung Department of Obstetrics and GynaecoIogy The University of Hong KongPokfulam Road Institute of molecular Biology The University of Hong Kong Pokfulam Road Department of Surgery The University of Hong KongPokfulam Road Department of Obstetrics and Gynaecology The University of Hong Kong Pokfulam Road

来自输卵管的胚胎滋养因子(ETF)-3,可促进滋养外胚层的发育和治疗胚胎的孵化过程。为了判定 ETF-3,我们制备了抗 ETF-3的单克隆抗体,并发现可去除 ETF-3的胚胎滋养活性。该抗体可识别来自于永久传代的人类输卵管细胞的特殊培养

关键词：细胞来源 ETF-3

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World Journal of Gastroenterology 2010年第3期16卷 289-295页

作者： Nikki P Lee john m.luk Department of Surgery Queen Mary Hospital Department of Pharmacology and Department of Surgery National University Health SystemNational University of Singapore

The tight junction (TJ) is a critical cellular component for maintenance of tissue integrity, cellular interactions and cell-cell communications, and physiologically functions as the "great wall" against external agents and the surrounding hostile environment. During the host-pathogen evolution, viruses somehow found the key to unlock the gate for their entry into cells and to exploit and exhaust the host cells. In the liver, an array of TJ molecules is localized along the bile canaliculi forming the blood-biliary barrier, where they play pivotal roles in paracellular permeability, bile secretion, and cell polarity. In pathology, certain hepatic TJ molecules mediate virus entry causing hepatitis infection; deregulation and functional abnormality of the TJ have also been implicated in triggering liver cancer development and metastasis. All these findings shed new insights on the understanding of hepatic TJs in the development of liver disease and provide new clues for potential intervention.

关键词： Tight junctions Hepatocytes Blood-biliary barrier Bile canaliculi Hepatitis Liver steatosis Liver neoplasms

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血浆蛋白质组模式预测鼠不同时期肝纤维化

中华肝脏病杂志 2006年第9期14卷 695-697页

作者：张其胜 john m.luk 上海市第一人民医院分院消化科 200081 香港大学医学院玛丽医院外科

蛋白质组模式在肿瘤性疾病的诊断方面应用已经较多，显示了良好的应用前景，但关于蛋白质组模式在肝纤维化时的改变较少有文献报道。为寻找一种新的、理想的诊断肝纤维化的方法，我们制备了不同时期肝纤维化鼠模型，用蛋白芯片技术检测... 详细信息

蛋白质组模式在肿瘤性疾病的诊断方面应用已经较多，显示了良好的应用前景，但关于蛋白质组模式在肝纤维化时的改变较少有文献报道。为寻找一种新的、理想的诊断肝纤维化的方法，我们制备了不同时期肝纤维化鼠模型，用蛋白芯片技术检测鼠血浆标本，根据血浆蛋白模式建立不同时期肝纤维化判别函数，并用鼠肝纤维化模型验证该判别函数的准确性。

关键词：肝纤维化蛋白质组模型,动物表面增强激光解吸电离技术

Suppressing ringing caused by large photomultiplier tube signals

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Chinese Physics C 2012年第3期36卷 235-240页

作者：蒋文奇顾树棣 john Joseph 刘大伟 Kam-Biu luk Herbert Steiner 王铮吴群 University of Science and Technology of China Institute of High Energy Physics Chinese Academy of Sciences Lawrence Berkeley National Laboratory University of Illinois at Urbana-Champaign University of California Illinois Institute of Technology

We describe here the characteristic features of the ringing we observed following large PmT signals in the Daya Bay reactor antineutrino experiment. We conclude that the ceramic capacitors used in the circuitry of the PmT bases and the HV-signal decouplers are the primary cause for this ringing. We present some possible schemes to reduce the ringing when replacing these ceramic capacitors is not feasible.

关键词： PmT ringing piezoelectric Daya Bay antineutrino

Role of LPS/CD14/TLR4-mediated inflammation in necrotizing enterocolitis:Pathogenesis and therapeutic implications

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World Journal of Gastroenterology 2009年第38期15卷 4745-4752页

作者： Kwong L Chan Kwong F Wong john m.luk Department of Surgery The University of Hong Kong Division of Paediatric Surgery Queen Mary Hospital Department of Pharmacology and Department of Surgery National University Health SystemNational University of SingaporeMD11 #05-1210 Medical Drive

AIm:To establish the roles of lipopolysaccharide (LPS)/CD14/toll-like receptor 4 (TLR4)-mediated inflammation in a rat model of human necrotizing enterocolitis (NEC).mETHODS: Six pairs of intestinal samples from human NEC were collected before and after recovery for histological and molecular analysis of inflammatory cytokines and signaling components. In the rat NEC model, we isolated 10-cm jejunum segments and divided them into six groups (n=6) for sham operation, treatment with LPS, bowel distension, combined bowel distension and LPS stimulation, and two therapeutic groups. The potential eff icacy of a recombinant CD18 peptide and a monoclonal CD14 antibody was evaluated in the latter two groups. The serum and tissue levels of several inflammatory mediators were quantified by real-time polymerase chain reaction, ELISA and ***: Human acute phase NEC tissues displayed significant increases (Pmyeloid differentiation protein (mD)-2, tumor necrosis factor (TNF)-α and nuclear factor-κB when compared to those after recovery. The histological and inflammatory picture of human NEC was reproduced in rats that were treated with combined bowel distension and LPS, but not in the sham-operated and other control rats. Serum levels of interleukin-6 and TNF-α were also elevated. The NEC pathology was attenuated by treating the NEC rats with a monoclonal CD14 antibody or an LPS-neutralizing ***:LPS and distension are required to produce the histological and inflammatory features of NEC. A potential treatment option is blocking LPS activation and leukocyte infi ltration.

关键词： CD14 antigen Lipopolysaccharide Necrotizing enterocolitis Pathogenesis Therapy Toll-like receptor 4

Genomics and Proteomic Biomarkers of Liver Cancer

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Genomics and Proteomic Biomarkers of Liver Cancer

Dickkopfs and Wnt/β-catenin signalling in liver cancer

中华医学会第九次全国检验医学学术会议暨中国医院协会临床检验管理专业委员会第六届全国临床检验实验室管理学术会议

作者： john m.luk Departments of Pharmacology and Surgery and Cancer Science Institute of Singapore National University of Singapore

Hepatocellular carcinoma(HCC) affects millions of people worldwide and is a lethal malignancy for which there are no effective *** identify prognostic gene markers for liver cancer,we conducted transcriptome profiling of frozen tissues(tumor and non-tumor) from 300 early-to-advanced stage HCCs plus 40 cirrhotic and 6 normal *** expression analysis reveals that as HCC progresses normal liver metabolic functions are impaired,whereas in cirrhotic liver proinflammatory/fibrogenic processes such as wound healing,extracellular matrix remodeling

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World Journal of Clinical Oncology 2011年第8期2卷 311-325页

作者： Sarwat Fatima Nikki P Lee john m.luk Department of Surgery The University of Hong KongHong KongChina Department of Pharmacology and Surgery National University Health SystemSingapore 117597Singapore Cancer Science Institute of Singapore National University of SingaporeSingapore 117456Singapore Institute of molecular and Cell Biology A*STAR SingaporeSingapore 138673Singapore

Liver cancer is the fifth and seventh most common cause of cancer in men and women,***/β-catenin signalling has emerged as a critical player in both the development of normal liver as well as an oncogenic driver in hepatocellular carcinoma(HCC).Based on the current understanding,this article summarizes the possible mechanisms for the aberrant activation of this pathway with specific focus on ***,we will discuss the role of dickkopfs(DKKs)in regulating Wnt/β-catenin signalling,which is poorly understood and *** are a family of secreted proteins that comprise at least four members,namely DKK1-DKK4,which act as inhibitors of Wnt/β-catenin ***,not all members antagonize Wnt/β-catenin *** functional significance in hepatocarcinogenesis remains to be further characterized for which these studies should provide new insights into the regulatory role of DKKs in Wnt/β-catenin signalling in hepatic *** of the important oncogenic roles,there are an increasing number of therapeutic molecules targetingβ-catenin and the Wnt/β-catenin pathway for potential therapy of HCC.

关键词： Dickkopf Hepatocellular carcinoma Tumourigenesis Wnt/β-catenin signalling

甘草次酸靶向肝星状细胞治疗肝纤维化的体内研究

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中华肝脏病杂志 2005年第9期13卷 664-667页

作者：张其胜 john m.luk 张健田庚元上海市第一人民医院分院消化科 200081 香港大学医学院玛丽医院外科中国科学院上海有机化学研究所

目的观察以6-磷酸甘露糖修饰的白蛋白(m6P26-HSA)作为特异性载体,将甘草次酸(GA) 靶向释放到肝星状细胞治疗肝纤维化的效果。方法用125I记由m6P26-HSA和GA在体外合成新的偶合物GA-HSA-m6P26,观察其在体内的器官分布情况,用双重免疫组织... 详细信息

目的观察以6-磷酸甘露糖修饰的白蛋白(m6P26-HSA)作为特异性载体,将甘草次酸(GA) 靶向释放到肝星状细胞治疗肝纤维化的效果。方法用125I记由m6P26-HSA和GA在体外合成新的偶合物GA-HSA-m6P26,观察其在体内的器官分布情况,用双重免疫组织化学的方法观察星状细胞对GA- HSA-m6P26的选择性摄取;选用Sirius红染色观察GA-HSA-m6P26对肝纤维化时胶原沉积的影响,用定量聚合酶链反应检测GA-HSA-m6P26对Ⅰ型前胶原mRNA表达的影响。结果静脉注射后10min,GA-HSA- m6P26选择性地分布于肝脏,摄取高峰可达(5 5.093±5.404)%。双重免疫组织化学染色证实GA-HSA- m6P26主要被星状细胞选择性摄取,GA-HSA-m6P26治疗后肝脏胶原沉积明显减少,Ⅰ型前胶原和α-平滑肌肌动蛋白mRNA表达明显降低。结论GA-HSA-m6P26可以选择性地分布于肝脏星状细胞,有显著的抗肝纤维化作用。

关键词：甘草次酸肝纤维化 6-磷酸甘露糖肝星状细胞甘草次酸(GA) 抗肝纤维化作用体内研究细胞治疗靶向释放双重免疫组织化学染色

Prophylactic uses of integrin CD18-βA peptide in a murine polymicrobial peritonitis model

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World Journal of Gastroenterology 2010年第21期16卷 2648-2656页

作者： Kwong-Fai Wong Jana Wo David Ho Ronnie T Poon José m Casasnovas john m.luk Department of Pharmacology and Surgery National University Hospital SystemNational University of SingaporeSingapore 117597Singapore Department of Surgery Queen Mary HospitalPokfulamHKSARHong KongChina Centro Nacional de Biotecnología CSICCampus Universidad Autónoma28049 MadridSpain

AIm:To evaluate the prophylactic properties of integrin CD18-βA peptide in a murine model of abdominal polymicrobial peritonitis and ***:Bacterial sepsis was induced in Institute of Cancer Research(ICR) mice by cecal ligation and puncture(CLP) *** mice were then injected with either sterile saline or CD18-βA peptide intraperitoneally at 2 h after surgery,and were sacrificed at 12 and 24 h after *** samples were immediately collected,and analyzed for endotoxin activity and tumor necrosis factor(TNF)-α and interleukin(IL)-*** and liver were studied for CD45+ leukocyte and CD3 mRNA *** expression of intercellular adhesion molecule(ICAm)-1,vascular cell adhesion molecule(VCAm) and E-selectin was also ***:Intraperitoneal injection of CD18-βA peptide significantly suppressed circulating endotoxin activity(Pm levels of TNF-α(PmRNA ***,the peptide significantly reduced pulmonary expression of VCAm(Pm-1(Pmice against lethality(P<0.01).CONCLUSION:CD18-βA peptide is a potent endotoxin antagonist that can protect surgical patients against sepsis-associated lethality.

关键词： Bacterial endotoxin Integrin CD18 Inflammation Biotherapeutic peptide