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Classification of Soybean Pubescence from Multispectral Aerial Imagery

Plant Phenomics 2021年第1期3卷 156-166页

作者： Robert W.Bruce Istvan Rajcan john sulik Department of Plant Agriculture University of GuelphGuelphONCanada

The accurate determination of soybean pubescence is essential for plant breeding programs and cultivar ***,soybean pubescence is classified visually,which is a labor-intensive and time-consuming ***,the three classes of phenotypes(tawny,light tawny,and gray)may be difficult to visually distinguish,especially the light tawny class where misclassification with tawny frequently *** objectives of this study were to solve both the throughput and accuracy issues in the plant breeding workflow,develop a set of indices for distinguishing pubescence classes,and test a machine learning(ML)classification approach.A principal component analysis(PCA)on hyperspectral soybean plot data identified clusters related to pubescence classes,while a Jeffries-Matusita distance analysis indicated that all bands were important for pubescence class *** images from 2018,2019,and 2020 were analyzed in this study.A 60-plot test(2019)of genotypes with known pubescence was used as reference data,while whole-field images from 2018,2019,and 2020 were used to examine the broad applicability of the classification *** indices,a red/blue ratio and blue normalized difference vegetation index(blue NDVI),were effective at differentiating tawny and gray pubescence types in high-resolution imagery.A ML approach using a support vector machine(SVM)radial basis function(RBF)classifier was able to differentiate the gray and tawny types(83.1%accuracy and kappa=0:740 on a pixel basis)on images where reference training data was *** tested indices and ML model did not generalize across years to imagery that did not contain the reference training panel,indicating limitations of using aerial imagery for pubescence classification in some environmental ***-throughput classification of gray and tawny pubescence types is possible using aerial imagery,but light tawny soybeans remain difficult to classify and may require training da

关键词： indices spectral consuming

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Empty nose syndrome pathogenesis and cell-based biotechnology products as a new option for treatment

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World Journal of Stem Cells 2021年第9期13卷 1293-1306页

作者： Inna M Gordiienko Olga S Gubar Roman sulik Taras Kunakh Igor Zlatskiy Alona Zlatska Biotechnology Laboratory Medical Company“Good Cells”Kyiv 03115Ukraine R.E.Kavetsky Institute of Experimental Pathology Oncology and Radiobiology NAS of UkraineKyiv 03022Ukraine Institute of Molecular Biology and Genetics NAS of Ukraine Kyiv 03143Ukraine State Institute of Genetic and Regenerative Medicine National Academy of Medical Sciences of UkraineKyiv 04114Ukraine

Empty nose syndrome (ENS) is a rare complication that develops after partial orcomplete turbinectomy. The main feature of ENS is paradoxical nasal obstructionfeeling despite objectively wide nasal airway. ENS pathogenesis is multifactorialand includes changes in laminar physiological airflow, disruption of mucosafunctions and deficient neural sensation. This leads to the development of ENSsymptomatology such as dyspnea, nasal dryness, nasal burning, nasalobstruction, feeling of suffocation and even comorbid psychiatric disorders thatsignificantly impairs life quality. Specific effective treatment of ENS does not existup to date. In this review we outline existing biomaterial for surgical reconstitutionof nasal anatomy and discuss the perspective of stem cell-based technologiesin ENS management. The main focus is directed to justification ofrationality application of adult mesenchymal stem cells (MSCs) from differenttissues origin and neural crest-derived stem cells (NCSCs) based on their intrinsicbiological properties. MSCs transplantation may stimulate mucosa tissueregeneration via trophic factors secretion, direct transdifferentiation into epithelialcells and pronounced immunosuppressive effect. From the other hand, NCSCsbased on their high neuroprotective properties may reconstitute nerve structureand functioning leading to normal sensation in ENS patients. We postulate thatapplication of cell-based and tissue-engineered products can help to significantlyimprove ENS symptomatology only as complex approach aimed at reconstitutionof nasal anatomy, recovery the nasal mucosa functionality and neural tissuesensation.

关键词： Empty nose syndrome Stem-cell-based technologies Implants biomaterials Grafts Hydrogels Mesenchymal stem cells

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