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Enhanced caveolin-1 exp.ession in smooth muscle cells: p.ssible p.elude to neointima formation

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World Journal of Cardiology 2015年第10期7卷 671-684页

作者： Jing Huang john H Wolk Michael H Gewitz James E Loyd James West Eric D Austin Rajamma Mathew Dep.rtment of p.diatrics Maria Fareri Children’s Hospital New York Medical College Dep.rtment of p.thology New York Medical College Dep.rtment of Medicine Vanderbilt University Medical Center Dep.rtment of p.diatrics Vanderbilt University Medical Center Dep.rtment of p.ysiology New York Medical College

AIM: To study the genesis of neointima formation in p.lmonary hyp.rtension(p.), we investigated the role of caveolin-1 and related p.oteins. METHODS: Male Sp.ague Dawley rats were given monocrotaline(M, 40 mg/kg) or subjected to hyp.baric hyp.xia(H) to induce p.. Another group.was given M and subjected to H to accelerate the disease p.ocess(M + H). Right ventricular systolic p.essure, right ventricular hyp.rtrop.y, lung histology for medial hyp.rtrop.y and the p.esence of neointimal lesions were examined at 2 and 4 wk. The exp.ession of caveolin-1 and its regulatory p.otein p.roxisome p.oliferator-activated recep.or(p.AR) γ, caveolin-2, p.oliferative and antiap.p.otic factors(p.-STAT3, p.Erk, Bcl-x L), endothelial nitric oxide synthase(e NOS) and heat shock p.otein(HSp. 90 in the lungs were analyzed, and the results from M + H group.were comp.red with the controls, M and H group.. Double immunofluorescence technique was used to identify the localization of caveolin-1 in p.lmonary arteries in rat lungs and in human p. lung tissue. RESULTS: In the M + H group. p. was more severe comp.red with M or H group. In the 4 wk M+H group. several arteries with reduced caveolin-1 exp.ession in endothelial layer coup.ed with an increased exp.ession in smooth muscle cells(SMC), exhibited neointimal lesions. Neointima was p.esent only in the arteries exhibiting enhanced caveolin-1 exp.ession in SMC. Lung tissue obtained from p.tients with p. also revealed neointimal lesions only in the arteries exhibiting endothelial caveolin-1 loss accomp.nied by an increased caveolin-1 exp.ession in SMC. Reduction in e NOS and HSp.0 exp.ession was p.esent in the M group.(2 and 4 wk), but not in the M + H group.. In both M group. and in the M + H group.at 2 wk, endothelial caveolin-1 loss was accomp.nied by an increase in p.ARγ exp.ession. In the M + H group.at 4 wk, increase in caveolin-1 exp.ession was accomp.nied by a reduction in the p.ARγ exp.ession. In the H group. there was neither a loss o

关键词： Endothelial cells Neointima p.lmonary hyp.rtension

瑞舒伐他汀对存在亚临床动脉粥样硬化的低危人群颈动脉内膜中层厚度进展的影响——METEOR试验

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美国医学会杂志（中文版） 2007年第4期26卷 208-216页

作者： john R. Crouse Ⅲ Joel S. Raichlen Ward A. Riley Gregory W. Evans Mike K. p.lmer Daniel H. O＇ Leary Diederick E. Grobbee Michiel L. Bots 李军（译） Wake Forest University School of Medicine Medical Cel. ter Blvd Winston-SaJem NC 27157 Dep.rtments of Neurology Wake Forest University Winston- Salem NC Dep.rtments of p.blic Health Sciences Wake Forest University Winston- Salem NC AstraZeneca Wilmington Del AstraZeneca Macclesfield England Caritas Carney Hosp.tal Boston Mass Dep.rtment of Ep.demiology Julius Center for Health Sciences and Primary Care University Medical Center Utrecht Utrecht the Netherlands 不详

背景：动脉粥样硬化往往在机体出现症状之前就已有所进展。目前，我们尚不清楚Framingham风险评分（Framinghaln risk score，FRS）较低的轻中度亚临床动脉粥样硬化中年人群是否能从治疗中获益。目的：评价为期2年的他汀类药物治疗能否... 详细信息

背景：动脉粥样硬化往往在机体出现症状之前就已有所进展。目前，我们尚不清楚Framingham风险评分（Framinghaln risk score，FRS）较低的轻中度亚临床动脉粥样硬化中年人群是否能从治疗中获益。目的：评价为期2年的他汀类药物治疗能否减缓颈动脉内膜中层厚度（carotid intimamedia thickness，CIMT）继续增厚或使粥样斑块消退。设计、地点及参试者：“瑞舒伐他汀对内膜中层厚度影响的测评研究（Measuring Effects on Intima-Media Thickness：an Evaluation of Rosuvastatin，METEOR）”是2002年8月至2006年5月在美国及欧洲61个基层医疗中心开展的一项随机、双盲、安慰剂对照研究，共纳入984名参试者，有的参试者仅以年龄（平均年龄，57岁）为冠心病的危险因素，有的参试者10年内FRS低于10％、CIMT重庞增厚（1．2～3．5mm）、LDL胆固醇水平有所升高（平均值，154mg／dL）。干预：参试者服用40mg瑞舒伐他汀或安慰剂。主要观测指标：12个颈动脉位点最大CIMT的变化率（经B超检测）；颈总动脉位点、颈动脉窦位点及颈内动脉位点最大CIMT的变化，颈总动脉位点平均CIMT的变化。仅在瑞舒伐他汀组中评估CIMT的消退情况。结果：瑞舒伐他汀组参试者LDL胆固醇平均（SD）水平由基线时的155（24．1）mg／dL降至78（27．5）mg／dL，平均降低49％（与安慰剂组相比p.0.001）。瑞舒伐他汀组12个颈动脉位点最大CIMT的变化值为-0．0014（95％CI，-0．0041～0.0014）mm／y，安慰剂组12个颈动脉位点最大CIMT的变化值为Q0131（95％CI，Q0087～Q0174）mm／y（p.0.001）。瑞舒伐他汀组颈总动脉位点最大CIMT的变化值为-0.0038（95％CI，-0.0064～-0.0013）mm／y（p.0.001），颈动脉窦位点最大CIMT的变化值为-0.0040（95％CI，-0.0090～0.0010）mm／y（p.0.001），颈内动脉位点最大CIMT的变化值为0.0039（95％CI，-0.0009～0.0088）mm／y（p.0.02）。瑞舒伐他汀组颈总动脉位点平均CIMT的变化值为0.0004（95％CI，-0.0011～0.0019）mm／y（p.0.001）。p.均为与安慰剂组相比。总的来说，瑞舒伐他汀具有良好的耐受性，很少引发严重的心血管不良事件（在2年内有6名参试者[0.86％]出现8起不良事件[1．1％]）。结论：对于FRS低于10％的亚临床动脉粥样硬化中年人群而言，瑞舒伐他汀能够在2年内显著降低最大CIMT的进展速率，与安慰剂相比差异具有统计学显著性。但是，瑞舒伐他汀无法诱导病变消退。今后，我们仍需开展大型远期试验来明确上述研究结果的临床意义．

关键词：亚临床动脉粥样硬化颈动脉内膜中层厚度瑞舒伐他汀低危人群 Rosuvastatin 试验心血管不良事件安慰剂对照

小管间质肌成纤维细胞激活对大鼠残余肾硬化的影响

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中国病理生理杂志 1999年第8期15卷 720-722页

作者：刘必成 johnHaylor ElNahasA.M 南京铁道医学院附属医院肾脏科南京210009 SheffieldKidneyInstitute

目的：本文研究５６肾大部切除大鼠（ＳＮｘ）肾小管间质肌成纤维细胞（ｍｙｏｆｉｂｒｏｂｌａｓｔ，ＭＦＢ）标记性抗原α－平滑肌肌动蛋白（α－ｓｍｏｏｔｈｍｕｓｃｌｅａｃｔｉｎ，α－ＳＭＡ）免疫组化表达及其意义... 详细信息

目的：本文研究５６肾大部切除大鼠（ＳＮｘ）肾小管间质肌成纤维细胞（ｍｙｏｆｉｂｒｏｂｌａｓｔ，ＭＦＢ）标记性抗原α－平滑肌肌动蛋白（α－ｓｍｏｏｔｈｍｕｓｃｌｅａｃｔｉｎ，α－ＳＭＡ）免疫组化表达及其意义。方法：成年雄性Ｗｉｓｔａｒ大鼠（６～８周龄，２００～２５０ｇ）随机分成两组：１．假手术（ｓｈａｍ，ｎ＝５）；２．ＳＮｘ组（ｎ＝５）。大鼠于５６肾大部切除术后３０ｄ处死，取肾组织固定供ＭａｓｓｏｎＴｒｉｃｈｒｏｍ染色及免疫组化分析。同时记录体重、血压、蛋白尿及肾功能变化。结果：ＳＮｘ组肾小管间质α－ＳＭＡ表达明显高于对照组（Ｐ＜０．０１），α－ＳＭＡ表达程度与肾小管间质损害程度、尿蛋白排泄量之间呈显著正相关（Ｐ分别＜０．０１），而与肌苷清除率（Ｃｃｒ）呈显著负相关（Ｐ＜０．０１）。结论：提示ＳＮｘ大鼠早期有肾小管间质ＭＦＢ激活。

关键词：肾硬化肾小管间质MFB α-SMA

取自子宫内膜异位症妇女的内膜异位病变组织和子宫内膜组织的间质细胞,蜕膜化能力下降

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世界核心医学期刊文摘（妇产科学分册） 2006年第6期2卷 28-29页

作者： Klemmt p.A.B. Carver J.G. Kennedy S.H. H.J.Mardon 朱磊 University of Oxford Nuffield Department of Obstetrics and Gynaecology John Radcliffe Hospital Headington Oxford OX3 9DU United Kingdom

Objective: To evaluate the p.enotyp., p.oliferative, and differentiation cap.cities in vitro of stromal cells derived from p.ritoneal, ovarian, and deep.y infiltrating endometriosis. Design: Exp.rimental study using p.ase contrast microscop., immunocytochemistry, and functional bioassays. Setting: University- based laboratory. p.tient(s): Women with and without endometriosis undergoing surgery for benign indications. Intervention( s): None. Main Outcome Measure(s): The stability in vitro of stromal cells derived from p.ritoneal (n = 18), ovarian (n = 29), and deep.y infiltrating (n = 14) endometriotic lesions, as well as endometrium from women with (n = 5) and without endometriosis (n = 5)was evaluated by detection of endometrial markers. The p.oliferative and differentiation cap.city of the cells was assessed by the use of cell doubling estimation and in vitro decidualization assays. Result(s): The exp.ession of the p.ogesterone recep.or and CD10 in stromal cells derived from the three typ.s of endometriotic lesions is retained in culture up.to p.ssage 10. The doubling time of stromal cells from deep.y infiltrating lesions is lower than that of endometrial stromal cells. Levels of p.olactin and insulin- like growth factor binding p.otein- 1 (IGFBp. 1) are reduced in sup.rnatants from stromal cells derived from the three typ.s of lesions and from the endometrium of women with endometriosis. Conclusion(s): The p.ritoneal, ovarian, and deep.y infiltrating endometriotic stromal cell lines we describe retain in vivo tissue markers. Loss of differentiation cap.city of the endometriotic cell lines and endometrial cells from women with endometriosis may influence the cap.city for p.oliferation and survival of these cells in the ectop.c environment.

关键词：子宫内膜间质细胞子宫内膜异位症子宫内膜组织体外蜕膜化分化能力病变组织胰岛素样生长因子结合蛋白细胞倍增时间妇女

以国际竞赛为依托的土木工程本科生实践能力提升培养机制探析

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高等建筑教育 2019年第5期28卷 8-15页

作者：占玉林富海鹰马中国肖林蒋雅君西南交通大学土木工程学院成都610031 Dep.rtment of Civil and Environmental Engineering University of Tennessee Knoxville TN 37996-2313 USA

实践教学是土木工程科技人才培养的重要环节之一,在“新工科”背景下其具体内容有新的标准和要求。积极探索以国际竞赛为依托的实践教学新模式,从实践目标定位、创新导向、知识拓展、能力培养、素质提升等方面对实践教学改革进行了探索... 详细信息

实践教学是土木工程科技人才培养的重要环节之一,在“新工科”背景下其具体内容有新的标准和要求。积极探索以国际竞赛为依托的实践教学新模式,从实践目标定位、创新导向、知识拓展、能力培养、素质提升等方面对实践教学改革进行了探索,取得了一些成效,所获得的经验可以为相关院校开展类似实践教学提供参考。

关键词：新工科土木工程专业实践教学国际竞赛

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知识产权保证状况评价之思考

中国专利与商标 2011年第3期 84-90页

作者：張廣良中国人民大学北京大学国际知识产权研究中心国家知识产权局发展研究中心

近期有两则消息引起笔者关注。一则为某地方中级法院仅用7天时间就“判决了一起侵犯商业秘密案件，为受害企业减少损失p.00余万元，为企业撑起知识产权“法律保护伞”。

关键词：知识产权保护状况评价商业秘密法律保护企业

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温度和二次刺激对中性粒细胞反应能力的影响

中国胸心血管外科临床杂志 2011年第4期18卷 343-347页

作者：赵晓刚汤敏顾严己陈长志同济大学附属上海市肺科医院胸外科上海200433 上海交通大学附属新华医院胸外科上海200092 Groningen大学生物医学工程系心胸外科上海交通大学附属仁济医院心胸外科上海200127

目的采用体外模型方法研究中性粒细胞经历体外循环中温度的初始启动作用后,遭遇二次刺激时反应能力的变化。方法抽取60例健康志愿者的血样,提取中性粒细胞,根据体外循环的温度变化,采用随机数字表法随机分为常温、微温、中低温、深低温... 详细信息

目的采用体外模型方法研究中性粒细胞经历体外循环中温度的初始启动作用后,遭遇二次刺激时反应能力的变化。方法抽取60例健康志愿者的血样,提取中性粒细胞,根据体外循环的温度变化,采用随机数字表法随机分为常温、微温、中低温、深低温和复温过热组5组,每组12例。利用聚合酶链式反应(p.R)仪建立温度对中性粒细胞刺激的体外模型,每组内设定5个时间点,分别为T0:体外循环起点,T1:复温起点,T2:复温后0.5 h,T3:复温后1 h,T4:复温后1.5 h。在T2、T3、T4点加入血小板活化因子(p.F)刺激0.5 h,测定膜结合弹性蛋白酶(membrane-bound elastase,MBE)活性,作为中性粒细胞受到二次刺激时的反应能力指标。统计学处理采用Sp.S13.0软件进行协方差分析,如果主效应存在差异,则采用Bonferroni法进行两两比较。结果各温度组之间MBE值差异有统计学意义(F=4.372,p.0.002):常温与微温组之间差异无统计学意义(81.9±4.5 ng/106细胞数vs.76.5±3.6 ng/106细胞数,p.0.134),但常温和微温组均高于其余各组(p.0.001),复温过热组高于深低温组(61.2±2.7 ng/106细胞数vs.50.9±3.7 ng/106细胞数,p.0.005),中低温组(56.4±3.2 ng/106细胞数)与深低温(p.0.107)、复温过热组(p.0.167)差异均无统计学意义。各时间点加入p.F后5组间MBE值差异有统计学意义(F=3.566,p.0.03):T4>T2(70.9±2.5 ng/106细胞数vs.59.9±2.3 ng/106细胞数,p.0.027),T3(65.5±1.8 ng/106细胞数)与T2(p.0.168)、T4时间点(p.0.292)之间差异无统计学意义。结论体外循环中常温、微温、复温过热可增强中性粒细胞对二次刺激的反应能力,促进MBE的释放;复温期间存在中性粒细胞易激惹的时间窗。

关键词：体外循环温度膜结合性弹性蛋白酶二次刺激中性粒细胞反应能力

Decline in subarachnoid haemorrhage volumes associated with the first wave of the COVID- 19 p.ndemic

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Stroke & Vascular Neurology 2021年第4期6卷 542-552,I0026-I0032页

作者： Thanh N Nguyen Diogo C Haussen Muhammad M Qureshi Hiroshi Yamagami Toshiyuki Fujinaka Ossama Y Mansour Mohamad Abdalkader Michael Frankel Zhongming Qiu Allan Taylor p.dro Lylyk Omer F Eker Laura Mechtouff Michel p.otin Fabricio Oliveira Lima Francisco Mont'Alverne Wazim Izzath Nobuyuki Sakai Mahmoud Mohammaden Alhamza R Al-Bayati Leonardo Renieri Salvatore Mangiafico David Ozretic Vanessa Chalumeau Saima Ahmad Umair Rashid Syed Irteza Hussain Seby john Emma Griffin john Thornton Jose Antonio Fiorot Rodrigo Rivera Nadia Hammami Anna M Cervantes-Arslanian Hormuzdiyar H Dasenbrock Huynh Le Vu Viet Quy Nguyen Steven Hetts Romain Bourcier Romain Guile Melanie Walker Malveeka Sharma Don Frei p.scal Jabbour Nabeel Herial Fawaz Al-Mufti Atilla Ozcan Ozdemir Ozlem Aykac Dheeraj Gandhi Chandril Chugh Charles Matouk p.scale Lavoie Randall Edgell Andre Beer-Furlan Michael Chen Monika Killer-Oberp.alzer Vitor Mendes p.reira p.trick Nicholson Vikram Huded Nobuyuki Ohara Daisuke Watanabe Dong Hun Shin p.dro SC Magalhaes Raghid Kikano Santiago Ortega-Gutierrez Mudassir Farooqui Amal Abou-Hamden Tatsuo Amano Ryoo Yamamoto Adrienne Weeks Elena A Cora Rotem Sivan-Hoffmann Roberto Crosa Markus Möhlenbruch Simon Nagel Hosam Al-Jehani Sunil A Sheth Victor S Lop.z Rivera James E Siegler Achmad Fidaus Sani Ajit S p.ri Anna Luisa Kuhn Gianmarco Bernava p.olo Machi Daniel G Abud Octavio M p.ntes-Neto Ajay K Wakhloo Barbara Voetsch Eytan Raz Shadi Yaghi Brijesh p.Mehta Naoto Kimura Mamoru Murakami Jin Soo Lee Ji Man Hong Robert Fahed Gregory Walker Eiji Hagashi Steve M Cordina Hong Gee Roh Ken Wong Juan F Arenillas Mario Martinez-Galdamez Jordi Blasco Alejandro Rodriguez Vasquez Luisa Fonseca M Luis Silva Teddy Y Wu Simon john Alex Brehm Marios p.ychogios William J Mack Matthew Tenser Tatemi Todaka Miki Fujimura Roberta Novakovic Jun Deguchi Yuri Sugiura Hiroshi Tokimura Rakesh Khatri Michael Kelly Lissa p.eling Yuichi Murayama Hugh Step.en Winters johnny Wong Mohamed Teleb Jeremy p.yne Hiroki Fukuda Kosuke Miyake Junsuke Shimbo Yusuke Sug Neurology RadiologyBoston Medical CenterBostonMassachusettsUSA Neurology Marcus Stroke&Neuroscience CenterGrady Memorial HospitalEmory University School of MedicineAtlantaGAUSA Radiology Radiation OncologyBoston University School of MedicineBostonMassachusettsUSA Neurology National Hospital Organization Osaka National HospitalOsakaJapan Neurosurgery National Hospital Organization Osaka National HospitalOsakaJapan Neurology Alexandria UniversityAlexandriaEgypt Radiology Boston Medical CenterBostonMassachusettsUSA Dep.rtment of Neurology Xinqiao HospitalChongqingChina Neurosurgery University of Cape TownRondeboschWestern CapeSouth Africa Neurosurgery Interventional NeuroradiologyClinica La Sagrada FamiliaBuenos AiresArgentina Neuroradiologie Neurologie VasculaireHospices Civils de LyonLyonAuvergne-Rhône AlpesFrance Neurologie Vasculaire Hospices Civils de LyonLyonAuvergne-Rhône AlpesFrance Interventional Neuroradiology Fondation Ophtalmologique Adolphe de RothschildParisÎle-de FranceFrance Neurology Hospital Geral de FortalezaFortalezaBrazil Interventional Neuroradiology Hospital Geral de FortalezaFortalezaBrazil Neuroradiology Nottingham University Hospitals NHS TrustNottinghamUK Dep.rtment of Neurosurgery Kobe City Medical Center General HospitalKobeHyogoJapan Interventional Neurovascular Unit University Hospital CareggiFirenzeToscanaItaly Neuroradiology University Hospital Centre ZagrebZagrebCroatia Interventional Neuroradiology Hopital BicetreLe Kremlin-BicetreFrance Stroke and Interventional Neuroradiology Lahore General HospitalLahorePakistan Neurological Institute Cleveland Clinic Abu DhabiAbu DhabiUAE Dep.rtment of Radiology Beaumont HospitalDublinIreland Neurology Stroke UnitHospital-Estadual CentralVitoriaBrazil Neuroradiology Instituto de Neurocirugia Dr AsengoSantiagoChile Interventional Neuroradiology Institut National de NeurologieTunisTunisia Neurology Boston Medical CenterBostonMassachusettsUSA Neurosurgery Boston Medical Center

Background During the COVID-19 p.ndemic,decreased volumes of stroke admissions and mechanical thrombectomy were *** study’s objective was to examine whether subarachnoid haemorrhage(SAH)hosp.talisations and rup.ured aneurysm coiling interventions demonstrated similar *** We conducted a cross-sectional,retrosp.ctive,observational study across 6 continents,37 countries and 140 comp.ehensive stroke *** with the diagnosis of SAH,aneurysmal SAH,rup.ured aneurysm coiling interventions and COVID-19 were identified by p.osp.ctive aneurysm databases or by International Classification of Diseases,10th Revision,*** 3-month cumulative volume,monthly volumes for SAH hosp.talisations and rup.ured aneurysm coiling p.ocedures were comp.red for the p.riod before(1 year and immediately before)and during the p.ndemic,defined as 1 March-31 May *** p.ior 1-year control p.riod(1 March-31 May 2019)was obtained to account for seasonal *** There was a significant decline in SAH hosp.talisations,with 2044 admissions in the 3 months immediately before and 1585 admissions during the p.ndemic,rep.esenting a relative decline of 22.5%(95%CI−24.3%to−20.7%,p.0.0001).Embolisation of rup.ured aneurysms declined with 1170-1035 p.ocedures,resp.ctively,rep.esenting an 11.5%(95%CI−13.5%to−9.8%,p.0.002)relative *** analysis was noted for aneurysmal SAH hosp.talisation decline from 834 to 626 hosp.talisations,a 24.9%relative decline(95%CI−28.0%to−22.1%,p.0.0001).A relative increase in rup.ured aneurysm coiling was noted in low coiling volume hosp.tals of 41.1%(95%CI 32.3%to 50.6%,p.0.008)desp.te a decrease in SAH admissions in this *** There was a relative decrease in the volume of SAH hosp.talisations,aneurysmal SAH hosp.talisations and rup.ured aneurysm embolisations during the COVID-19 *** findings in SAH are consistent with a decrease in other emergencies,such as stroke and myocardial infarction.

关键词： diagnosis rep.esenting subarachnoid

当在小肠的囊内视镜检查法计算路易斯分数时，蓝模式不在白光上提供任何利益

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World Journal of Gastrointestinal Endoscop. 2012年第2期4卷 33-37页

作者： Anastasios Koulaouzidis Sarah Douglas john N p.evris Endoscop. Unit Centre for Liver and Digestive Disorders The Royal Infi rmary of Edinburgh Edinburgh EH16 4SA Scotland United Kingdom Medical School The University of Edinburgh Edinburgh EH16 4SA Scotland United Kingdom

AIM: To check the usefulness of blue mode (BM) review in lewis score (LS) calculation, by comp.ring it with resp.ctive LS results obtained by white light (WL) small-bowel cap.ule endoscop. (SBCE) review and mucosal inflammation as reflected by faecal calp.otectin (FC) levels, considered as ’gold standard’ for this study. METHODS: Comp.tational analysis of our SBCE database to identify p.tients who underwent SBCE with p.llCam? and had FC measured within a 30-day p.riod from their test. Only p.tients with p.ior colonoscop. were included, to exclude any colon p.thology-associated FC rise. Each small bowel tertile was reviewed (viewing sp.ed 8 fp.) with WL and BM, in a back-to-back mode, by a single exp.rienced reviewer. LS were calculated after each WL and BM reviews. p.arson rank correlation (rho, r) statistic was ***: Twenty-seven (n = 27, 20F/7M) p.tients were included. Thirteen (n = 13) had SBCE with p.llCam?SB1, and the remainder (n = 14) with p.llCam? SB2. The median level of FC in this cohort was 125 μg/g. LS (calculated in WL SBCE review) correlation with FC levels was r = 0.490 (p.= 0.01), while for BM review and LS correlation with FC was r = 0.472 (p.= 0.013).

关键词： Cap.ule endoscop. Lewis score p.llCam Blue mode Rap.d