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Electron microscopy combined with spatial analysis:quantitative mapping of the nano-assemblies of plasma membrane-associating proteins and lipids

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Biophysics Reports 2018年第6期4卷 320-328页

作者： Yong Zhou john f.Hancock Department of Integrative Biology and Pharmacology The University of Texas Health Science Center at Houston

The plasma membrane(PM) is a complex environment consisting of [ 700 species of lipids and many different types of membrane-associating proteins. These lipids and membrane proteins are distributed heterogeneously into nanometer-sized domains, called nanoclusters. The lateral spatial segregation in the PM gives rise to different curvature and lipid composition, which determines the efficiency of effector binding and signal transmission. Here, we describe an electron microscopy(EM)-spatial mapping technique to quantify the extent of nanoclusters formation in the PM. The nano-assemblies in the PM are quantified via expressing the GfP-tagged proteins or lipid-binding domains in the cells,which are then immunolabeled with the gold nanoparticles pre-coupled to the anti-GfP antibody. The gold nanoparticles are visualized via the transmission EM at high magnification. The statistical analysis of the Ripley's K-function calculates the spatial distribution of the gold nanoparticles. Important spatial parameters, such as the extent of nanoclustering, the clustered fraction, the number of proteins per cluster, the optimal size of a nanocluster, and the number of proteins localized to the PM, can be calculated. further detailed aggregation pattern, such as the populations of monomers, dimers, trimers,and higher ordered oligomers, can also be extracted from the spatial analysis. The EM-bivariate analysis quantifies the extent of co-localization between two different components in the PM and provides key information on the protein–protein and the protein–lipid interactions over a long-distance scale from 8 to 240 nm.

关键词： Electron microscopy Lipid-anchored proteins Nanoclustering Ripley’sK-function Signal transduction Spatial distribution

Selling Building Parterships Barton A. Weitz, Stephen B. Castleberry, john f. Tanner, Jr. 6th ed.

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Hospitalizations for alcoholic liver disease during the COVID-19 pandemic increased more for women,especially young women,compared to men

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作者： Barton A. Weitz Stephen B. Castleberry john f. Tanner Jr.

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World Journal of Hepatology 2023年第2期15卷 282-288页

作者： john Patterson Campbell Vinay Jahagirdar Adel Muhanna Kevin f Kennedy john H Helzberg Department of Internal Medicine Division of Gastroenterology and HepatologySaint Luke’s Health System of Kansas City and the University of Missouri-Kansas CityKansas CityMO 64111United States Department of Internal Medicine Saint Luke’s Health System of Kansas City and the University of Missouri-Kansas CityKansas CityMO 64111United States Division of Cardiology Saint Luke’s Health System of Kansas CityKansas CityMO 64111United States Department of Internal Medicine Division of Gastroenterology and HepatologyLiver Disease Management UnitSaint Luke’s Health System of Kansas City and the University of Missouri-Kansas CityKansas CityMO 64111United States

BACKGROUND Alcoholic liver disease(ALD)remains one of the major indications for liver transplantation in the United States and continues to place a burden on the national healthcare *** is evidence of increased alcohol consumption during the coronavirus disease 2019(COVID-19)pandemic,and the effect of this on the already burdened health systems remains *** To assess the trends for ALD admissions during the COVID-19 pandemic,and compare it to a similar pre-pandemic *** This retrospective study analyzed all admissions at a tertiary health care system,which includes four regional *** admissions were identified by querying a multi-hospital health system’s electronic database using ICD-10 *** admissions were compared for two one-year periods;pre-COVID-19 from April 2019 to March 2020,and during-COVID-19 from April 2020 to March *** were analyzed using a Poisson regression model and admission rates were compared using the annual quarterly average for the two time periods,with stratification by age and *** increase or decrease in admissions from the Poisson regression model were reported as incident rate *** One thousand three hundred and seventy-eight admissions for ALD were included.80.7%were Caucasian,and 34.3%were *** increase in the number of admissions for ALD during the COVID-19 pandemic was *** women,a sharp rise(33%)was noted in those below the age of 50 years,and an increase of 22%in those above 50 *** men,an increase of 24%was seen for those below 50 years,and a 24%decrease in those above 50 *** The COVID-19 pandemic has had widespread implications,and an increase in ALD admissions is just one of ***,given that women are often prone to rapid progression of ALD,this finding has important preventive health implications.

关键词： Alcoholic liver disease COVID-19 Alcoholic hepatitis Alcoholic liver cirrhosis Alcoholism Pandemic Young women Alcohol-related disorders

Contrasting female mate preferences for red coloration in a fish

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Current Zoology 2020年第4期66卷 425-433页

作者： Charel Reulaimd Brett M.CuLBERT Alessandro Devigili Ariel f.Kahrl john L.fitzpatrick Department of Zoology Stockholm UniversityStockholmSE-10691Sweden Department of Integrative Biology University of GuelphGuelphN1G 2W1Canada

Understanding how animals select their mates requires knowing the factors that shape mate *** theoretical and empirical considerations suggest that female mating status can influence the degree to which a female engages in mate choice,with virgin females predicted to be less choosy than mated *** this study,we investigated mate choice in both virgin and mated females in the pygmy halfbeak Dermogenys *** are small,live-bearing,internally fertilizing freshwater fish that live in mixed-sex groups where females have ample opportunity to engage in mate *** a dichotomous choice assay,we quantified and contrasted in virgin and mated females mate preferences for differences in male body size,beak size,and area of yellow and red *** also examined how mating status influenced the amount of time a female associated with the first male encountered and the relative amount of time a female associated with each *** demonstrate that mate preferences of female halfbeaks are driven primarily by the size of red coloration present on *** showed contrasting preferences based on mating status,with virgin females preferentially associating with drab males whereas mated females preferentially associate with males possessing large areas of *** to expectations,female mating status did not influence how females associate with the first males encountered or how females biased their association time among *** the precise drivers of these effects need further studying,our finding highlights a possible explanation for how variation in male ornamentation can be maintained.

关键词： Dermogenys collettei experience plasticity pygmy halfbeak reproductive status sexual selection sperm competition

Helicobacter pylori infection and extragastric disorders in children: A critical update

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World Journal of Gastroenterology 2014年第6期20卷 1379-1401页

作者： Lucia Pacifico john f Osborn Valeria Tromba Sara Romaggioli Stefano Bascetta Claudio Chiesa Department of Pediatrics Sapienza University of Rome Department of Health Sciences and Infectious Diseases Sapienza University of Rome Institute of Translational Pharmacology National Research Council

Helicobacter pylori(H. pylori) is a highly prevalent, serious and chronic infection that has been associated causally with a diverse spectrum of extragastric disorders including iron deficiency anemia, chronic idiopathic thrombocytopenic purpura, growth retardation, and diabetes mellitus. The inverse relation of H. pylori prevalence and the increase in allergies, as reported from epidemiological studies, has stimulated research for elucidating potential underlying pathophysiological mechanisms. Although H. pylori is most frequently acquired during childhood in both developed and developing countries, clinicians are less familiar with the pediatric literature in the field. A better understanding of the H. pylori disease spectrum in childhood should lead to clearer recommendations about testing for and treating H. pylori infection in children who are more likely to develop clinical sequelae. A further clinical challenge is whether the progressive decrease of H. pylori in thelast decades, abetted by modern clinical practices, may have other health consequences.

关键词： Helicobacter pylori Children Iron deficiency anemia Chronic idiopathic thrombocytopenic purpura Growth retardation Asthma Allergy Diabetes mellitus

“Digging in the Dirt”faecal microRNAs as dietary biomarkers of host-microbe interactions

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Hepatobiliary Surgery and Nutrition 2022年第2期11卷 292-294页

作者： Gerard M.Moloney john f.Cryan Gerard Clarke Department of Anatomy&Neuroscience University College CorkCorkIreland APC Microbiome Ireland University College CorkCorkIreland Department of Psychiatry and Neurobehavioural Science University College CorkCorkIreland

The role of diet and nutrition in maintaining a healthy lifestyle has become a central part of chronic disease prevention(1).Chronic conditions such as cancer,Inflammatory bowel disease(IBD)and obesity are more prevalent in people with poor diets(2).food consumption is controlled by physiological systems such as the gut-microbiome and distinct neuroendocrine pathways that overlap to promote host nutritional gain resulting in people living longer healthier lives(3).A recent analysis of the global burden of disease by the World Health Organization(WHO),noted that factors associated with poor diet,including obesity and malnutrition,critically undermine health and *** note that undernutrition,deficiencies in key nutrients and obesity have high social and economic *** disease,cancer,diabetes,and respiratory diseases account for 77%of all diseases and are responsible for 86%of all premature deaths(4).There is an appreciation that diets that reduce or eliminate red meats and animal fats results in the reduction of many of the indicators of poor diet,such as body mass index(BMI)and levels of cholesterol in the blood.

关键词： prevention eliminate overlap

Active surveillance monitoring： the role of nove biomarkers and imaging

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Asian Journal of Andrology 2015年第6期17卷 882-884,I0006页

作者： john f Ward Scott E Eggener Department of Urology The University of TexasMD Anderson Cancer Center Houston TX USA Department of Urology University of ChicagoChicago IL USA.

＂CANCER＂ is a disease state that leads to progressive illness that is uniformly fatal without treatment. Hippocrates invoked the Greek word karkinos, or ＂crab＂ to describe tumors he observed. for centuries, ＂CANCER＂ remained a disease that was recognized primarily in its locally advanced or metastatic stage, when it was almost uniformly fatal.

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Characterizing drought in California:new drought indices and scenario-testing in support of resource management

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Ecological Processes 2018年第1期7卷 1-13页

作者： Lorraine E.flint Alan L.flint john Mendoza Julie Kalansky f.M.Ralph U.S.Geological Survey 6000 J.StSacramentoCA 95819USA Sonoma County Water Agency 404 Aviation Dr.Santa RosaCA 95403USA Scripps Institution of Oceanography 9500 Gilman Dr.La JollaCA 92093USA

Introduction:California’s recent drought(2012–2016)has implications throughout the state for natural resource management and adaptation planning and has generated many discussions about drought characterization and *** study characterizes drought conditions with two indices describing deficits in natural water supply and increases in landscape stress developed on the basis of water balance modeling,at a fine spatial scale to assess the variation in conditions across the entire state,and provides an in-depth evaluation for the Russian River basin in northern California to address local resource management by developing extreme drought scenarios for consideration in planning and ***:We employed the USGS Basin Characterization Model to characterize drought on the basis of water supply(a measure of recharge plus runoff)and landscape stress(climatic water deficit).These were applied to the state and to the Russian River basin where antecedent soil moisture conditions were evaluated and extreme drought scenarios were developed and run through a water management and reservoir operations model to further explore impacts on water ***:Drought indices indicated that as of the end of water year 2016 when reservoirs were full,additional water supply and landscape replenishment of up to three average years of precipitation in some locations was needed to return to normal *** soil conditions in the Russian River were determined to contribute to very different water supply results for different years and were necessary to understand to anticipate proper watershed response to *** drought scenarios manifested very different kinds of drought and recovery and characterization helps to guide the management response to ***:These scenarios and indices illustrate how droughts differ with regard to water supply and landscape stress and how long warm droughts recover much more slowly than short very dry dro

关键词： California drought Russian River Water supply Basin Characterization Model

Role of Mg on Structure and Mechanical Properties in Alloy 718

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工程科学学报 1989年第6期11卷 560-567页

作者：徐志超 ,谢锡善 ,屈波 ,陈国良 ,Radavich john f 北京科技大学北京 School of Materials Engineering Purdue University

The role of Mg in alloy/718 has been systematically investigated. Mg raises not only high temperature tensile and stress-rupture ductilities but also increases considerably smooth and notch stress-rupture life. Mg containing alloy 718M is free of stress-rupture notch sensitivity. Mg improves creep and fatigue interaction properties (LCf or cyclic stress rupture) at any grain size. The basic role of Mg is equilibrium segregation at grain boundaries which helps to change continuous grain beundary (5-Ni3Nb morphology to discrete globular form which has a retardation effect on intergranular fracture. Mg promotes the change from intergranular to transgranular fracture mode.

关键词： superalloy creep and fatigue mechanical properties