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Progress in Natural Science:Materials International 2009年第5期19卷 581-586页

作者： john clamp Khaled A.S.Al-Rasheid Abdulaziz A.Al-Khedhairy Department of Biology North Carolina Central University Zoology Department King Saud University

Phylogenetic and taxonomic studies on ciliate protists using molecular approaches have been demonstrated to be very reliable to form strong conclusions and results. In the present work, species separation of some morphologically similar stichotrichous ciliates, two species of Pseudokeronopsis and two species of Apokeronopsis, was reexamined using amplified ribosomal DNA restriction analysis (PCR-RFLP). Five of 10 restriction enzymes revealed species-specific polymorphic patterns, of which four similar stichotrichs could be significantly separated and identified. Among them, EcoR I offered almost no significantly different restriction fragment patterns, but the four species could be separated from one another and identified with Hae Ⅲ. Distinctly different restriction digestion haplotypes and simi-larity indices separated the species, and were used to construct a phylogeny. Phylogenies based on ITS2 nucleotide sequences and ITS2 secondary structures supported the separation of Pseudokeronopsis and Apokeronopsis using RFLP analysis, although three Pseudoker-onopsis carnea populations did not cluster together. In addition, phylogenetic analyses using multiple algorithms confirmed that these two genera formed two distinct groups within the urostylids.

关键词： Ciliophora Pseudokeronopsis Apokeronopsis PCR-RFLP ITS2 secondary structures

Phylogenetic investigation on five genera of tintinnid ciliates (Ciliophora, Choreotrichia), based on the small subunit ribosomal RNA gene sequences

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Progress in Natural Science:Materials International 2009年第9期19卷 1097-1101页

作者： john C. clamp Khaled A.S. Al-Rasheid Saud Al-Arifi Abdulaziz A. Al-Khedhairy Weibo Song Department of Biology North Carolina Central University Zoology Department King Saud University Laboratory of Protozoology KLM Ocean University of China

The phylogenetic relationship among tintinnid ciliates are relatively poorly studied based on molecular data. In the present work, seven species belonging to five genera of the order Tintinnida (Amphorellopsis acuta, Codonellopsis nipponica, Favella taraikaensis, Steno-semella nivalis, Tintinnopsis beroidea, Tintinnopsis cylindrica and Tintinnopsis lohmanni) were analyzed using the information on their small subunit ribosomal RNA gene sequences. Phylogenetic trees were constructed using Bayesian inference (BI), maximum parsimony (MP), neighbor-joining (NJ), and least-squares (LS) methods. Generally, similar topologies were revealed with high or moderate supports, in which the main results show that (1) all tintinnids analyzed belong to a single assemblage; (2) congeners in Tintinnopsis do not cluster together, which indicates that the lorica-based definition for this genus is not consistent with the SSU rRNA phylogeny; (3) A. acuta groups with Tintinnidium mucicola but not with Eutintinnus, indicating that the traditional family Tintinnidae might be a paraphyletic group; (4) Stenosemella and Codonellopsis are clearly most related and possibly even merged into one genus regarding their similar morphology and molecular analyses, and possession of a hyaline collar is the only characteristic of the genus.

关键词： Phylogeny Tintinnida Molecular systematics SSU rRNA

寡毛类纤毛虫变游虫新属的建立及基尔变游虫(新属,新组合)的形态学重描述与系统地位分析(英文)

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动物分类学报 2011年第3期36卷 502-511页

作者：徐大鹏孙萍 johnC.clamp 马洪钢宋微波中国海洋大学生物多样性与进化研究所原生动物学研究室青岛2660032 Department of Biology North Carolina Central University

利用蛋白银染色技术对采自青岛沿海砂隙的寡毛类纤毛虫Strombidium kielum进行了形态学重描述,发现该种在寡毛类纤毛虫中具有独一无二的纤毛下器模式,因此为其建立了1新属Varistrombidium,特征为具有5条斜穿虫体的体动基列,其中体动基列... 详细信息

利用蛋白银染色技术对采自青岛沿海砂隙的寡毛类纤毛虫Strombidium kielum进行了形态学重描述,发现该种在寡毛类纤毛虫中具有独一无二的纤毛下器模式,因此为其建立了1新属Varistrombidium,特征为具有5条斜穿虫体的体动基列,其中体动基列1和2延伸到虫体背部,终止于虫体尾端。对Varistrombidium kielum(Maeda&Carey,1985)***.的小亚基RNA序列分析表明,该种位于Strombidiidae科内,与其形态学相近种Omegastrombidium elegans聚在一起。同时对其小亚基RNA序列可变区2的二级结构进行了预测并与其形态学相似种进行了比较。还对Apostrombidium pseudokielum Xuet al.,2009进行了补充性描述。

关键词：小亚基RNA 二级结构黄海中国

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SiC JFETs Modular Cascaded Method with Active clamp Control Strategy for DC Solid-state Circuit Breaker

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CSEE Journal of Power and Energy Systems 2023年第6期9卷 2435-2446页

作者： Hong Duan Wei Wang Zhikang Shuai Dong He Daixin Chen Z.john Shen the College of Electrical and Information Engineering Hunan UniversityChangsha 410006China the Department of Electrical and Computer Engineering Illinois Institute of TechnologyChicagoIL 60616 USA.

Power semiconductor devices cascaded topology is one of the most common solutions for solid-state circuit breakers(SSCBs)working in medium-voltage DC ***,when the number of cascaded devices is large,current series technologies of power semiconductor devices will be difficult to be applied since with uneven voltage sharing ***,this paper proposes a novel cascade method of multi-SiC JFETs based on modules *** method consists of two parts:one is a single-gate driver cascaded SiC JFETs topology which is used to form the module,the other one is an active clamp control strategy which ensures that each module is protected from overvoltage when modules are *** proposed cascade method can effectively suppress voltage overshoot of cascading power devices in the switching *** on the proposed cascade method,a 5kV/63A SSCB prototype is ***,an experiment is conducted based on the designed *** results validate the effectiveness of the proposed cascaded method for SSCB.

关键词： Active control clamp DC solid-state circuit breaker modular SiC JFETs series connection

Estrogen augmented visceral pain and colonic neuron modulation in a double-hit model of prenatal and adult stress

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World Journal of Gastroenterology 2021年第30期27卷 5060-5075页

作者： Jing-Hong Chen Ying Sun Pei-Jun Ju Jin-Bao Wei Qing-Jie Li john H Winston Shanghai Key Laboratory of Psychotic Disorders Shanghai Mental Health CenterShanghai Jiao Tong University School of MedicineShanghai 200030China Department of Internal Medicine University of Texas Medical BranchGalvestonTX 77555United States

BACKGROUND Chronic stress during pregnancy may increase visceral hyperalgesia of offspring in a sex-dependent *** adult stress in offspring will increase this *** on the evidence implicating estrogen in exacerbating visceral hypersensitivity in female rodents in preclinical models,we predicted that chronic prenatal stress(CPS)+chronic adult stress(CAS)will maximize visceral hyperalgesia;and that estrogen plays an important role in colonic *** The aim was to illuminate the role of estrogen in colonic hyperalgesia and its underlying *** We established a CPS plus CAS rodent model in which the balloon was used to distend the *** single-fiber recording in vivo and patch clamp experiments in vitro were used to monitor the colonic neuron’s *** reverse transcription-polymerase chain reaction,western blot,and immunofluorescence were used to study the effects of CPS and CAS on colon primary afferent *** used ovariectomy and letrozole to reduce estrogen levels of female rats respectively in order to assess the role of estrogen in female-specific enhanced primary afferent *** Spontaneous activity and single fiber activity were significantly greater in females than in *** enhanced sensitization in female rats mainly came from lowthreshold *** significantly increased single-unit afferent fiber activity in L6-S2 dorsal roots in *** was further enhanced by *** addition,the excitability of colon-projecting dorsal root ganglion(DRG)neurons increased in CPS+CAS rats and was associated with a decrease in transient Atype K+*** with ovariectomy,treatment with the aromatase inhibitor letrozole significantly reduced estrogen levels in female rats,confirming the gender ***,mice treated with letrozole had decreased colonic DRG neuron *** intrathecal infusion of estrogen increased brain-derived neurotrophic factor(BDNF)p

关键词： Chronic prenatal stress Estrogen Visceral pain Neuronal sensitization Excitability Letrozole

Cyclopiazonic acid增加SHR和WKY血管平滑肌细胞Ca^(2+)-依赖性外向K^+-电流

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中国药理学通报 1993年第4期9卷 257-260页

作者：关永源 john Zhang Lee RMKW Kwan CY Daniel EE 中山医科大学药理教研室 Section of Neurosurgery Medical CenterThe University of ChicagoChicagoIllinois 60637 USA Smooth Musele Research Program Departments of Anacthesia and Biomedical SciencesMcMaster UniversityHamiltonOntario Canada L8N 3Z5

采用全细胞及细胞贴附式斑片钳技术记录自发性高血压大鼠(SHR)和Wistar-Kyoto对照鼠(WKY)培养主动脉平滑肌细胞的Ca^(2+)-依赖性外向K^+电流[I_(k(Ca))],测定肌浆网Ca^(2+)泵抑制剂CPA对其影响.CPA能增加I_K(Ca))单通道开放时间,缩短关... 详细信息

采用全细胞及细胞贴附式斑片钳技术记录自发性高血压大鼠(SHR)和Wistar-Kyoto对照鼠(WKY)培养主动脉平滑肌细胞的Ca^(2+)-依赖性外向K^+电流[I_(k(Ca))],测定肌浆网Ca^(2+)泵抑制剂CPA对其影响.CPA能增加I_K(Ca))单通道开放时间,缩短关闭时间,增加全细胞I_(K(Ca))幅度,这些作用与Ca^(2+)相关并可被K^+通道阻断药glybenclamide阻断。CPA作用在SHR和WKY之间无明显差异。结果提示高血压状态下血管平滑肌的功能改变可能与I_(K(Ca))无关。

关键词： CPA 钾离子通道血管平滑肌细胞

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Synaptic calcium signaling in cochlear hair cells

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Synaptic calcium signaling in cochlear hair cells

Upregulation of transient receptor potential vanilloid 1 contributes to hyperalgesia in rats with painful chronic pancreatitis

中国神经科学学会第四次会员代表大会暨第七届全国学术会议

作者： Paul Albert Fuchs john E.Bordley Department of Otolaryngology Neck SurgeryJohns Hopkins University School of MedicineBaltimoreMarylandUSA

＜正＞Sensory hair cells of vertebrates convert mechanical energy into electrical signals for transmission to the central nervous *** hair cells also are postsynaptic to efferent neurons that project from the central nervous system to inhibit peripheral *** ions are essential cytoplasmic mediators of both afferent and efferent synaptic ***,like other so-called ’short’ receptors（taste cells,photoreceptors） hair cells are physically compact, possessing neither axons nor dendrites to provide spatially-segregated calcium signals for separate synaptic processes. Therefore,afferent excitation and efferent inhibition,often occurring at immediately adjacent synaptic sites,must rely on strong,specialized regulatory mechanisms that confer opposite functional significance to their specific calcium signals （reviewed in Fuchs and Parsons,2006）.Small conductance（SK） calcium-sensitive potassium channels are activated by release of ACh from efferent ***-clamp recording from cochlear hair cells can examine the underlying dynamics of synaptic calcium signaling through activation of these synapse-specific potassium *** altering cytoplasmic calcium buffering,or the efficacy of internal calcium stores,we have explored the mechanisms that regulate SK channel activity at efferent synaptic *** such experiments,it is clear that SK channel gating depends on release from internal calcium stores,presumably the ’synaptoplasmic cistern’ lying immediately adjacent to,and coextensive with,the efferent ***,this synaptoplasmic cistern can be loaded by influx through voltage-gated calcium channels, suggesting that it also may be involved in regulating calcium signals at afferent synapses as ***,the specialized synaptic calcium signaling of hair cells relies on energy-dependent,near-membrane calcium stores that help to segregate local calcium signals.

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Upregulation of transient receptor potential vanilloid 1 con...

Esthesioneuroblastoma Masquerading as Chronic Rhinosinusitis

中国神经科学学会第四次会员代表大会暨第七届全国学术会议

作者： john H WINSTON Mohan SHENOY Pankaj Jay PASRICHA Division of Gastroenterology and Hepatology Department of Internal MedicineUniversity of Texas Medical BranchGalvestonTexas 77555USA

＜正＞The neurobiological basis of pancreatic hyperalgesia in chronic pancreatitis（CP） is poorly understood and treatment of pain in this condition remains empirical and often *** have previously developed a rat model of trinitrobenzene sulphonic acid（TNBS）-induced chronic pancreatitis that results in nociceptive sensitization in rats manifested by pancreatic hyperalgesia and referred somatic *** aim of this study was to investigate the molecular substrate of these changes,specifically focusing on the role of the transient receptor potential vanilloid subfamily 1 （TRPV1）.CP was induced in adult rats by a single intraductal injection of *** root ganglion（DRG） neurons innervating the pancreas were identified by DiI or cholera B toxin fluorescence ***1 currents in pancreasspecific DRG neurons were measured using perforated patch-clamp ***-PCR was used to measure mRNA expression of TRPV1 in pancreas-specific DRG neurons after laser capture microdissection（LCM）.Immunofluorescence and western blot analysis,using TRPV1-specific antibodies,were also *** hyperalgesia was assessed by observing nocifensive behaviors after electrical stimulation of the pancreas and belly withdrawal responses were also recorded after von Fray Filament *** weeks after injection of TNBS,Capsaicin-induced current density of pancreas specific DRG neurons was increased about four-folds（P ＜ 0.02）,along with an increase in proportion of pancreas -specific DRG neurons that responded to capsaicin（52.9%in controls versus 79.0%in TNBS,P ＜ 0.05）.CP was also associated with a significant enhancement of TRPV1 expression both at the mRNA and protein level,including western blots in whole thoracic DRG and immunoreactivity in pancreas specific sensory *** administration of the TRPV1 antagonist（SB-366791） markedly reduced the pain behavioral response to noxious electrical stimulation of pancreas and von Frey Filament stimulation

关键词： Chronic pancreatitis transient receptor potential vanilloid subfamily 1 rat

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Case Reports in Clinical Medicine 2015年第9期4卷 303-308页

作者： Sylvia G. McCulloch Harold Y. Lau Yves Starreveld john T. Lysack Desiree Hao Department of Internal Medicine Faculty of Medicine University of Calgary Calgary Canada Tom Baker Cancer Center/University of Calgary Calgary Canada Division of Neurosurgery Foothills Medical Centre Calgary Canada Clinical Neurosciences and Surgery University of Calgary Department of Diagnostic Imaging Foothills Medical Centre Calgary Canada

Background: Esthesioneuroblastoma, a rare tumour of the nasal vault, can present with symptoms that can be mistaken for common conditions such as chronic rhinosinusitis or recurrent epistaxis. Case presentation: A 52-year-old, previously healthy, Caucasian female presented to the emergency room with two months of nasal congestion and a spontaneous, protracted nosebleed that required a nasal clamp to settle. In the following months she had another episode of epistaxis in addition to fatigue, headaches, anosmia, difficulty breathing, worsening vision, weight loss and behavioral changes. Subsequent investigations led to the diagnosis of esthesioneuroblastoma. Conclusion: When patients present with recurrent epistaxis or chronic rhinosinusitis that does not respond to a reasonable course of medical therapy and/or have atypical associated symptoms, further investigations should be considered. The presences of red flags, such as weight loss, severe headaches, vision changes or bizarre behavior are also clues that alternate diagnoses and further additional workup should be considered.

关键词： Esthesioneuroblastoma Sinonasal Neoplasms Sinusitis Epistaxis

Non-genetic photoacoustic stimulation of single neurons by a tapered fiber optoacoustic emitter

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Light(Science & Applications) 2021年第8期10卷 1494-1506页

作者： Linli Shi Ying Jiang Fernando R.Fernandez Guo Chen Lu Lan Heng-Ye Man john A.White Ji-Xin Cheng Chen Yang Department of Chemistry Boston University580 Commonwealth AvenueBostonMA 02215USA. Department of Biomedical Engineering Boston University44 Cummington MallBostonMA 02215USA. Center for Systems Neuroscience Boston University610 Commonwealth AveBostonMA 02215USA. Neurophotonics Center Photonics CenterBoston University8 St.Mary's StreetBostonMA 02215USA. Department of Electrical and Computer Engineering 8 St.Mary's StreetBostonMA 02215USA. Department of Biology Boston University5 Cummington MallBostonMA 02215USA

Neuromodulation at high spatial resolution poses great significance in advancing fundamental knowledge in the field of neuroscience and offering novel clinical ***,we developed a tapered fiber optoacoustic emitter(TFOE)generating an ultrasound field with a high spatial precision of 39.6 pm,enabling optoacoustic activation of single neurons or subcellular structures,such as axons and ***,a single acoustic pulse of sub-microsecond converted by the TFOE from a single laser pulse of 3 ns is shown as the shortest acoustic stimuli so far for successful neuron *** precise ultrasound generated by the TFOE enabled the integration of the optoacoustic stimulation with highly stable patch-clamp recording on single *** measurements of the electrical response of single neurons to acoustic stimulation,which is difficult for conventional ultrasound stimulation,have been *** coupling TFOE with ex vivo brain slice electrophysiology,we unveil cell-type-specific responses of excitatory and inhibitory neurons to acoustic *** results demonstrate that TFOE is a non-genetic single-cell and sub-cellular modulation technology,which could shed new insights into the mechanism of ultrasound neurostimulation.

关键词： emitter fiber stimulation