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Polymorphisms in salivary-gland transcripts of Russian wheat aphid biotypes 1 and 2

Insect Science 2012年第4期19卷 429-440页

作者： Feng Cui Michael Smith john reese Owain Edwards Gerald Reeck Department of Biochemistry Kansas State UniversityManhattanKansasUSA State Key Laboratory of Integrated Managementof Pest Insects&Rodents Institute of ZoologyChinese Academy of SciencesBeijingChina Department of Entomology Kansas StateUniversityManhattanKansasUSA CSIRO Entomology Canberra CooperaEve Research Centre for Plant Biosecufity CanberraAustralia

The Russian wheat aphid （RWA）, Diuraphis noxia （Mordvilko）（Homoptera： Aphididae）, is a major pest of small grains. As with plant-feeding aphids in general, the interaction between RWA and host plants is governed, on the insect side, by proteins and enzymes in saliva. In this work, we examined sequence variations in transcripts encoding proteins and enzymes of RWA salivary glands. We conducted reverse transcription - polymerase chain reaction in RWA biotypes 1 and 2 using primers derived from pea aphid orthologs, and cloned regions of 17 putative salivary gland transcripts. For four of the transcripts, we observed no difference in sequences between the two biotypes. For the other 13 transcripts, for example, the transcripts encoding sucrase, trehalase and protein C002, large amount of variations, both within each biotype and between the two biotypes, were observed. Usually the two biotypes shared only one variant, which was typically the most common variant in both biotypes. Most of the transcripts had more non-synonymous than synonymous codon changes among their variants. Our results offer possible molecular markers for distinguishing the two biotypes and insights into their evolution.

关键词： biotype molecular marker polymorphism Russian wheat aphid salivarygland transcript

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Genes encoding a group of related small secreted proteins from the gut of Hessian fly larvae [Mayetiola destructor （Say）]

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Insect Science 2006年第5期13卷 339-348页

作者： MING-SHUN CHEN XIANG LIU YU-CHENG ZHU john C. reese GERALD E. W ILDE USDA-ARS Plant Science and Entomology Research UnitUSA Department of Entomology Kansas State University Manhattan KansasUSA USDA-ARS-JWDSRC Stoneville Minnesota USA

A group of related genes has been isolated and characterized from the gut of Hessian fly larvae [Mayetiola destructor （Say）]. Members in this group appear to encode proteins with secretary signal peptides at the N-terminals. The mature putative proteins are small, acidic proteins with calculated molecular masses of 14.5 to 15.3 kDa, and isoelectric points from 4.56 to 4.88. Northern blot analysis revealed that these genes are expressed predominantly in the gut of Hessian fly larvae and pupae. Two related genes, GIOK1 and GIOK2, were isolated as tandem repeats. Both genes contain three exons and two introns. The intron/exon boundaries were conserved in terms of amino acid encoding, suggesting that they arose by gene duplication. The fact that the frequency of this group of clones in a gut cDNA library higher than that of total cDNA clones encoding digestive enzymes suggested that this group of proteins may perform an important function in the gut physiology of this insect. However, the exact functions of these proteins are as yet known since no sequence similarity could be identified between these proteins and any known sequences in public databases using standard methods.

关键词： Hessian fly gut Mayetiola destructor secreted protein wheat

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Predictive value of pituitary tumor morphology on outcomes and complications in endoscopic transsphenoidal surgery

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World Journal of Otorhinolaryngology-Head and Neck Surgery 2022年第4期8卷 321-329页

作者： Andrew T.Heffernan Joseph K.Han john Campbell James reese William G.Day Joshua Edwards Ran V.Singh Wylie Zhu Kent K.Lam Department of Otolaryngology-Head and Neck Surgery Eastern Virginia Medical SchoolNorfolkVirginiaUSA Medical Center Radiologists NorfolkVirginiaUSA Department of Radiology Eastern Virginia Medical SchoolNorfolkVirginiaUSA Sentara Healthcare Analytics and Delivery Science Institute NorfolkVirginiaUSA Neurosurgical Associates NorfolkVirginiaUSA Sentara Neurosurgery Specialists NorfolkVirginiaUSA

Purpose:Endoscopic transsphenoidal surgery(ETSS)is an increasingly utilized approach for resection of pituitary *** studies have evaluated preoperative tumor size,location,and extent as prognostic factors for surgical *** is little data on the relationship between preoperative pituitary tumor radiographic morphology and surgical *** Design:Retrospective longitudinal ***:Single tertiary care *** and Methods:Preoperative magnetic resonance imaging and computed tomography scans from patients undergoing ETSS for pituitary tumor resections from 2007 to 2017 were retrospectively evaluated.A neuroradiologist classified these pituitary tumors into six morphologic groups,each defined by volume,dimensions,extension,and *** difficulty,rates of incomplete resection,and postoperative complications were then stratified in relation to the morphologic ***:Pituitary tumors from 131 patients were classified from preoperative imaging into six characteristic morphologies:(1)microtumor,(2)round,(3)transverse oblong,(4)superior-inferior oblong,(5)bilobed,and(6)large *** that were characterized with the large lobulated,bilobed,and transverse oblong morphologies correlated with higher rates of postoperative evidence of residual tumor(70%,36%,and 47%,respectively,all P<0.002).Likewise,large lobulated,bilobed,and transverse oblong morphologies were also associated with intraoperative cerebrospinal fluid leaks(70%,31%,and 35%,respectively,all P<0.05).Conclusions:We describe a novel descriptive system for the morphology of pituitary tumors that can be determined from preoperative *** tumor morphologic groups are associated with varying degrees of gross tumor resection,complications,and surgical *** pituitary tumor morphology may aid surgeons in planning the extent of resection,need for complex closure,and patient counseling.

关键词： endoscopic transsphenoidal surgery morphology pituitary adenoma pituitary tumor surgical outcomes tumor shape

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