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Docosahexaenoic acid suppresses arachidonic acid-induced proliferation of LS-174T human colon carcinoma cells

World Journal of Gastroenterology 2009年第9期15卷 1079-1084页

作者： Piet Habbel Karsten H Weylandt Katja Lichopoj johannes nowak Martin Purschke Jing-Dong Wang Cheng-Wei He Daniel C Baumgart Jing X Kang Department of Medicine Massachusetts General Hospital and Harvard Medical School Boston MA 02114 United States Department of Medicine Division of Gastroenterology and Hepatology Charité Medical Center-Virchow-Hospital Medical School of the Humboldt-University of Berlin 13344 Berlin Germany

AIM:To investigate the impact of arachidonic acid (AA) and docosahexaenoic acid (DHA) and their combination on colon cancer cell ***:The LS-174T colon cancer cell line was used to study the role of the prostaglandin precursor AA and the omega-3 polyunsaturated fatty acid DHA on cell growth. Cell viability was assessed in XTT assays. For analysis of cell cycle and cell death,flow cytometry and DAPI staining were applied. Expression of cyclooxygenase-2 (COX-2),p21 and bcl-2 in cells incubated with AA or DHA was examined by real-time RT-PCR. Prostaglandin E2 (PGE2) generation in the presence of AA and DHA was measured using a ***:AA increased cell growth,whereas DHAreduced viability of LS 174T cells in a time-and dose-dependent manner. Furthermore,DHA down-regulated mRNA of bcl-2 and up-regulated p21. Interestingly,DHA was able to suppress AA-induced cell proliferation and significantly lowered AA-derived PGE2 formation. DHA also down-regulated COX-2 expression. In addition to the effect on PGE2 formation,DHA directly reduced PGE2-induced cell proliferation in a dose-dependent manner. CONCLUSION:These results suggest that DHA can inhibit the pro-proliferative effect of abundant AA or PGE2.

关键词：二十二碳六烯酸人结肠癌细胞花生四烯酸诱导扫描仪 LS 酶联免疫吸附试验前列腺素E2 酸抑制

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Angiographic Iongterm follow-up after coronary stent implantation in acute myocardial infarction

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中国介入心脏病学杂志 1998年第4期6卷 156-156页

作者： Ailaiti Maimaitiming Hans-Jrgen Rupprecht Flex Post Bernd nowak Jrgen Meyer Medical Clinic 2 Johannes Gutenberg University Mainz.Germany

Stent implantation in acute myocardial infarction(AMI)has been shown to improvethe acute angiographic *** far few data on long-term angiographic follow-up are ***:We studied 130 consecutive patients(mean age 58±11,24 women)betweenOctober 1993 and October 1997,in whom coronary stonts were implanted afterunsuccessful percutaneous transluminal coronary angioplasty (PTCA)in *** coronary angiography(QCA)was performed and residual stenosis wasmesuremesured for all patients immediately and for 74 patients at a mean of 6 months afterthat ***:The infarct related artery was the left anterior dascending in 60(46%)pts,the circumflex in 15(12%)and the right coronary artery in 55(42%).At baseline,72(55%)pts had a totally occluded artery(TLMI0)and 11(8%)TIMI 1 flow. Eight(6%) pte were given aboiximab(ReoPro) or thrombolyais to deal with acute slantthrombosis during the *** after stent implantation,angiographicsuccess(TLMI 3)was obtained in 113(87%)pts,TLMI2 in 14 (11%)and TLMI 1 intwo (2%). Only three pts had residual stonosis(≥50% stenosis)immediately *** the patients who had coronary artery bypass grafting(CABG)ordied coronary angiographic follow-up was performed in 74(70%)pts at a mean of 6months following the intervention(Table).Conclusion:Rescue stent implantation after failed PTCA in AMI may improve initialangiographic results,but we found a higher restenosis-rate and reintervention-rate inthe long-term run as compared with selectiv stent implantation.

关键词： stent implantation stenosis infarct immediately bypass grafting baseline implanted angioplasty

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