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Effects of undigested protein-rich ingredients on polarised small intestinal organoid monolayers

journal of Animal Science and Biotechnology 2020年第4期11卷 1026-1032页

作者： Soumya K.Kar Bart van der Hee Linda M.P.Loonen Nico taverne johanna j.taverne-thiele Dirkjan Schokker Mari A.Smits Alfons j.M.jansman jerry M.Wells Host Microbe Interactomics Group Wageningen University&ResearchDe Elst 16708 WD WageningenThe Netherlands Laboratory of Microbiology Wageningen University&ResearchStippeneng 46708 WE WageningenThe Netherlands Wageningen Livestock Research Wageningen University&ResearchDe Elst 16708 WD WageningenThe Netherlands

Here, we describe the use of monolayers of intestinal epithelial cells derived from intestinal organoids and transcriptomics to investigate the direct effects of dietary protein sources on epithelial function. Mechanically dissociated 3 D organoids of mouse duodenum were used to generate a polarized epithelium containing all cell types found in the tissue of origin. The organoid-derived cell monolayers were exposed to 4%(w/v) of ‘undigested(non-hydrolysed)-soluble' fraction of protein sources used as feed ingredients [soybean meal(SBM) and casein], or alternative protein sources(spray dried plasma protein, and yellow meal worm), or controls for 6 h prior to RNA isolation and transcriptomics. All protein sources altered expression of unique biological processes in the epithelial cells. Exposure of intestinal organoids to SBM downregulated expression of retinol and retinoid metabolic processes as well as cholesterol and lipid biosynthetic pathways, consistent with the reported hypotriglyceridaemic effect of soy protein in vivo. These findings support the use of intestinal organoids as models to evaluate complex interactions between dietary ingredients and the intestinal epithelium and highlights some unique host effects of alternative protein sources in animal feed and potentially human food.

关键词： Alternative protein sources Casein Intestinal organoids Organoids Soybean meal Spray dried plasma protein Transcriptomics Yellow meal worm

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Genomic selection in plant breeding:Key factors shaping two decades of progress

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Molecular Plant 2024年第4期17卷 552-578页

作者： Admas Alemu johanna Astrand Osval A.Montesinos-López julio Isidro y Sanchez javier Fernandez-Gónzalez Wuletaw Tadesse Ramesh R.Vetukuri Anders S.Carlsson Alf Ceplitis joséCrossa Rodomiro Ortiz Aakash Chawade Department of Plant Breeding Swedish University of Agricultural SciencesAlnarpSweden Lantmannen Lantbruk SvalövSweden Facultad de Telematica University de ColimaColimaColima 28040Mexico Centro de Biotecnología y Genómica de Plantas(CBGP UPM-INIA)Universidad Politécnica de Madrid(UPM)-Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria(INIA)Campus de Montegancedo-UPM28223 MadridSpain International Center for Agricultural Research in the Dry Areas(ICARDA) RabatMorocco International Maize and Wheat Improvement Center(CIMMYT) Km 45Carretera México-VeracruzTexcocoMéxico 52640Mexico

Genomic selection,the application of genomic prediction(GP)models to select candidate individuals,has significantly advanced in the past two decades,effectively accelerating genetic gains in plant *** article provides a holistic overview of key factors that have influenced GP in plant breeding during this *** delved into the pivotal roles of training population size and genetic diversity,and their relationship with the breeding population,in determining GP *** emphasis was placed on optimizing training population *** explored its benefits and the associated diminishing returns beyond an optimum *** was done while considering the balance between resource allocation and maximizing prediction accuracy through current optimization *** density and distribution of single-nucleotide polymorphisms,level of linkage disequilibrium,genetic complexity,trait heritability,statistical machine-learning methods,and non-additive effects are the other vital *** wheat,maize,and potato as examples,we summarize the effect of these factors on the accuracy of GP for various *** search for high accuracy in GP—theoretically reaching one when using the Pearson’s correlation as a metric—is an active research area as yet far from optimal for various *** hypothesize that with ultra-high sizes of genotypic and phenotypic datasets,effective training population optimization methods and support from other omics approaches(transcriptomics,metabolomics and proteomics)coupled with deep-learning algorithms could overcome the boundaries of current limitations to achieve the highest possible prediction accuracy,making genomic selection an effective tool in plant breeding.

关键词： genomic selection genetic gain genomic prediction optimization deep learning training population optimization

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Finding stable multi-component materials by combining cluster expansion and crystal structure predictions

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npj Computational Materials 2023年第1期9卷 2137-2146页

作者： Adam Carlsson johanna Rosen Martin Dahlqvist Materials Design Department of PhysicsChemistryand Biology(IFM)Linköping UniversitySE-58183 LinköpingSweden

A desired prerequisite when performing a quantum mechanical calculation is to have an initial idea of the atomic positions within an approximate crystal *** atomic positions combined should result in a system located in,or close to,an energy ***,designing low-energy structures may be a challenging task when prior knowledge is scarce,specifically for large multi-component systems where the degrees of freedom are close to *** this paper,we propose a method for identification of low-energy crystal structures within multi-component systems by combining cluster expansion and crystal structure predictions with density-functional theory *** structure prediction searches are applied to the Mo_(2)AlB_(2) and Sc2AlB_(2) ternary systems to identify candidate structures,which are subsequently used to explore the quaternary(pseudo-binary)(Mo_(x)Sc_(1–x))2AlB_(2) system through the cluster expansion formalism utilizing the ground-state search ***,we show that utilizing low-energy structures found within the cluster expansion ground-state search as seed structures within crystal structure predictions of(Mo_(x)Sc_(1–x))2AlB_(2) can significantly reduce the computational *** this combined approach,we not only correctly identified the recently discovered Mo_(4/3)Sc_(2/3)AlB_(2) i-MAB phase,comprised of in-plane chemical ordering of Mo and Sc and with Al in a Kagomélattice,but also predict additional low-energy structures at various *** result demonstrates that combining crystal structure prediction with cluster expansion provides a path for identifying low-energy crystal structures in multi-component systems by employing the strengths from both frameworks.

关键词： structure cluster crystal

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What are the pathways between poverty and malaria in sub-Saharan Africa?A systematic review of mediation studies

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Infectious Diseases of Poverty 2023年第3期12卷 13-30页

作者： Solomon T.Wafula Theresa Habermann Mara Anna Franke jürgen May Dewi Ismajani Puradiredja Eva Lorenz johanna Brinkel Department of Infectious Disease Epidemiology Bernhard Nocht Institute for Tropical MedicineHamburgGermany Department of Disease Control and Environmental Health School of Public HealthMakerere UniversityKampalaUganda Charité Global Health Charité Universitätsmedizin BerlinBerlinGermany German Center for Infection Research(DZIF) Partner Site Hamburg-Borstel-Lubeck-RiemsHamburgGermany Department of Tropical Medicine University Medical Center Hamburg-Eppendorf(UKE)HamburgGermany Institute of Medical Biostatistics Epidemiology and InformaticsUniversity Centre of the Johannes Gutenberg UniversityMainzGermany

Background Malaria remains a major burden in sub-Saharan Africa(SSA).While an association between poverty and malaria has been demonstrated,a clearer understanding of explicit mechanisms through which socioeconomic position(SEP)influences malaria risk is needed to guide the design of more comprehensive interventions for malaria risk *** systematic review provides an overview of the current evidence on the mediators of socioeconomic disparities in malaria in *** We searched PubMed and Web of Science for randomised controlled trials,cohort,case-control and cross-sectional studies published in English between january 1,2000 to May 31,*** studies were identified following reviews of reference lists of the studies *** included studies that either(1)conducted a formal mediation analysis of risk factors on the causal pathway between SEP and malaria infections or(2)adjusted for these potential mediators as confounders on the association between SEP and malaria using standard regression *** least two independent reviewers appraised the studies,conducted data extraction,and assessed risk of bias.A systematic overview is presented for the included *** We identified 41 articles from 20 countries in SSA for inclusion in the final *** these,30 studies used cross-sectional design,and 26 found socioeconomic inequalities in malaria *** formal mediation analyses showed limited evidence of mediation of food security,housing quality,and previous antimalarial ***,education,insecticide-treated nets,and nutrition were highlighted in the remaining studies as being protective against malaria independent of SEP,suggesting potential for ***,methodological limitations included the use of cross-sectional data,insufficient confounder adjustment,heterogeneity in measuring both SEP and malaria,and generally low or moderate-quality *** studies considered exposure mediator interactions or considered iden

关键词： Malaria Mediation Socioeconomic disparity Sub-Saharan Africa

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Estimation of drag forces caused by natural woody vegetation of different scales

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journal of Hydrodynamics 2014年第4期26卷 608-623页

作者： jALONEN johanna jRVEL juha Department of Civil and Environmental Engineering Aalto University

To reliably estimate water levels and velocities in vegetated rivers and floodplains, flow resistance models based on physical plant properties are advantageous. The purpose of this study is （1） to assess the suitable parameterization of woody riparian vegetation in estimating the drag forces, （2） to address the effect of plant scale on the drag estimates and reconfiguration, and （3） to evaluate the applicability of three recently developed flow resistance models. Experiments on four tree species in a towing tank together with detailed characterization of tree properties were carried out to establish a novel dataset. Despite the variability in the tree height （0.9 m-3.4 m）, the stem, leaf and total areas proved to be suitable characteristic dimensions for estimating the flow resistance at different scales. Evaluations with independent data revealed that the tested models produced reasonable results. The performance of the models was controlled by the parameter values used rather than the model structure or the plant scale.

关键词： drag force flow resistance woody vegetation modelling

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Experimental approaches to select tree species for forest restoration:effects of light,water availability and interspecific competition in degraded areas

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journal of Forestry Research 2022年第4期33卷 1197-1207页

作者： johanna Croce Ernesto I.Badano Carolina B.Trigo Fernanda Martinez-Galvez Andrés Tálamo Laboratorio de Ecología Aplicada a la Conservación Instituto de Bio y Geociencias del Noroeste ArgentinoConsejo de Investigaciones Científi cas y Técnicas-Universidad Nacional de SaltaAvenida Bolivia 51504400 SaltaArgentina IPICYT/División de Ciencias Ambientales Instituto Potosino de Investigación Científi ca y TecnológicaCamino a la Presa San José2055Colonia Lomas 4^(a)Sección78216 San Luis PotosíMéxico

Knowing what native trees can recr uit on degraded areas allows selecting the best species to restore these ***,as this information is not often available,experimentation is required before large-scale *** study used ex situ experiments to make these decisions on *** with r-strategist plants,excessive solar radiation and water shortage commonly impair tree recruitment in open *** experiments focused on the interactions among these factors and were conducted with three pioneer species from seasonally dry forests of northwest Argentina,Anadenanthera colubrina,Ceiba chodatii and jacaranda *** of each species were sown at two light levels(sunlight/shade),two rainfall levels(full/reduced)and two levels of interspecific competition(with/without competitor)in a tree *** emergence and survival were monitored over a year and the results indicate that species differentially respond to varying levels of light and *** of *** tolerated water shortages under elevated solar radiation,which are desirable features for forest *** of *** tolerated shade and drought,suggesting that they require shading for establishing in open ***,*** seedlings neither tolerated full sunlight nor water shortages,suggesting that this species requires shading and regular watering if used in *** of the effects of light and water,the survival of all species was reduced by interspecific *** results highlight the importance of experimentation for selecting the best species for forest restoration and can enhance the cost/benefit ratios of these actions.

关键词： Common-garden experiments Drought Ecological restoration Interspecific competition Seedling survival Shade

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The clinical impact of tricuspid regurgitation in patients with a biatrial orthotopic heart transplant

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Frontiers of Medicine 2023年第3期17卷 527-533页

作者： Kevin M.Veen Grigorios Papageorgiou Casper F.Zijderhand Mostafa M.Mokhles jasper j.Brugts Olivier C.Manintveld Alina A.Constantinescu jos A.Bekkers johanna j.Makkenberg Ad j.j.CBogers Kadir Caliskan Department of Cardiothoracic Surgery Erasmus MC3000 CA RotterdamThe Netherlands Department of Biostatistics Erasmus MC3000 CA RotterdamThe Netherlands Department of Cardiology Erasmus MC3000 CA RotterdamThe Netherlands

In this study,we aim to elucidate the clinical impact and long-term course of tricuspid regurgitation(TR),taking into account its dynamic nature,after biatrial orthotopic heart transplant(OHT).All consecutive adult patients undergoing biatrial OHT(1984−2017)with an available follow-up echocardiogram were ***-models were used to model the evolution of *** mixed-model was inserted into a Cox model in order to address the association of the dynamic TR with *** total,572 patients were included(median age:50 years,males:74.9%).Approximately 32% of patients had moderate-to-severe TR immediately after ***,this declined to 11%on 5 years and 9% on 10 years after surgery,adjusted for survival *** mechanical support was associated with less TR during follow-up,whereas concurrent LV dysfunction was significantly associated with more TR during *** at 1,5,10,20 years was 97%±1%,88%±1%,66%±2% and 23%±2%,*** presence of moderate-to-severe TR during follow-up was associated with higher mortality(HR:1.07,95%CI(1.02–1.12),p=0.006).The course of TR was positively correlated with the course of creatinine(R=0.45).TR during follow-up is significantly associated with higher mortality and worse renal ***,probability of TR is the highest immediately after OHT and decreases ***,it may be reasonable to refrain from surgical intervention for TR during earlier phase after OHT.

关键词： orthotopic heart transplant tricuspid regurgitation clinical impact biatrial heart transplantation

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Dietary zinc and metallothionein on small intestinal disaccharidases activity in mice

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World journal of Gastroenterology 2011年第3期17卷 354-360页

作者： Cuong D Tran johanna Cool Cory j Xian Gastroenterology Unit ChildrenYouth and Women's Health Service Discipline of Physiology School of Medical SciencesThe University of Adelaide Discipline of Agricultural and Animal Science School of AgricultureFood and Wine The University of Adelaide Adelaide Sansom Institute for Health Research School of Pharmacy and Medical SciencesUniversity of South Australia

AIM: To examine the effect of increasing dietary zinc (Zn) intake and the lack of metallothionein (MT) expression on activity of small intestinal disaccharidases. METHODS: MT-Ⅰ and Ⅱ knockout (MT-/-) and wild-type (MT+/+) female mice at 3.5 wk of age were randomly fed with a diet containing 2 (2 Zn), 15 (15 Zn) or 50 (50 Zn) mg Zn/kg (n = 8/group/genotype) for 5 wk. Small intestinal segments (duodenum, jejunum and ileum) were collected and either fixed in 10% formalin for histological analysis or snap frozen in liquid nitrogen for sucrase, lactase and maltase activity ***: Plasma Zn was significantly (P jejunal sucrase and lactase activities were significantly higher in MT-/- (13 313 ± 2314; 4107 ± 364 μmol glucose/well/min/g tissue, respectively) compared with MT+/+ mice (7054 ± 608; 1818 ± 174). Similarly, ileal lactase activities were higher in MT-/- (1480 ± 192) compared with MT+/+ (629 ± 353) mice particularly those fed the 2 Zn diet. CONCLUSION: Increasing dietary Zn has little effect on disaccharidases activity in MT wild-type mice. The presence of MT may enhance morphological and functional development of the gut.

关键词：雌性小鼠金属硫蛋白活性分析蔗糖酶小肠膳食锌十二指肠

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Analysis of human brain tissue derived from DBS surgery

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Translational Neurodegeneration 2022年第1期11卷 625-640页

作者： Salla M.Kangas jaakko Teppo Maija j.Lahtinen Anu Suoranta Bishwa Ghimire Pirkko Mattila johanna Uusimaa Markku Varjosalo jani Katisko Reetta Hinttala PEDEGO Research Unit University of OuluOuluFinland Medical Research Center Oulu University HospitalUniversity of OuluOuluFinland Biocenter Oulu University of OuluOuluFinland Institute of Biotechnology HiLIFE Helsinki Institute of Life ScienceUniversity of HelsinkiHelsinkiFinland Drug Research Program Faculty of PharmacyUniversity of HelsinkiHelsinkiFin-land Neurosurgery Research Unit of Clinical NeuroscienceOulu University HospitalUniversity of OuluOuluFinland Oulu Research Group of Advanced Surgical Technologies and Physics(ORGASTP) Research Unit of Clinical NeuroscienceOulu University HospitalUniversity of OuluOuluFinland Institute for Molecular Medicine Finland(FIMM) HiLIFE Helsinki Institute of Life ScienceUniversity of HelsinkiHelsinkiFinland Clinic for Children and Adolescents Division of Pediatric NeurologyOulu University HospitalOuluFinland

Background:Transcriptomic and proteomic profiling of human brain tissue is hindered by the availability of fresh samples from living *** samples usually represent the advanced disease stage of the ***-thermore,the postmortem interval can affect the transcriptomic and proteomic ***,fresh brain tissue samples from living patients represent a valuable resource of metabolically intact *** of deep brain stimulation(DBS)electrodes into the human brain is a neurosurgical treatment for,e.g.,movement ***,we describe an improved approach to collecting brain tissues from surgical instruments used in implantation of DBS device for transcriptomics and proteomics ***:Samples were extracted from guide tubes and recording electrodes used in routine DBS implantation procedure to treat patients with Parkinson’s disease,genetic dystonia and *** sequencing was performed in tissues extracted from the recording microelectrodes and liquid chromatography-mass spectrometry(LC-MS)per-formed in tissues from guide *** assess the performance of the current approach,the obtained datasets were compared with previously published datasets representing brain ***:Altogether,32,034 RNA transcripts representing the unique Ensembl gene identifiers were detected from eight samples representing both hemispheres of four *** using LC-MS,we identified 734 unique proteins from 31 samples collected from 14 *** datasets are available in the BioStudies database(accession number S-BSST667).Our results indicate that surgical instruments used in DBS installation retain brain material sufficient for protein and gene expression *** with previously published datasets obtained with similar approach proved the robustness and reproducibility of the ***:The instruments used during routine DBS surgery are a useful source for obtaining fresh brain tis-sues from living patien

关键词： Deep brain stimulation Movement disorders Brain Proteomics Transcriptomics RNA sequencing LC-MS Personalized medicine

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Inflammatory Biomarkers in Elite Cross-Country Skiers After a Competition Season:A Case-Control Study

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journal of Science in Sport and Exercise 2023年第3期5卷 254-262页

作者： Raakel Luoto Olli Ruuskanen johanna K.Ihalainen Satu Pekkala jukka Hintikka Noora Kanerva Matti Waris Olli j.Heinonen Maarit Valtonen Department of Paediatrics and Adolescent Medicine Turku University Hospital and University of TurkuPL 5220521 TurkuFinland Faculty of Sport and Health Sciences University of JyväskyläJyväskyläFinland Department of Food and Nutrition University of HelsinkiHelsinkiFinland Institute of Biomedicine Department of Clinical VirologyUniversity of TurkuTurku University HospitalTurkuFinland Paavo Nurmi Centre and Unit for Health and Physical Activity University of TurkuTurkuFinland Finnish Institute of High Performance KIHURautpohjankatu640700 JyväskyläFinland

Purpose Whether elite athletes,who have been exposed to vigorous-intensity exercise combined with other stressors,have elevated systemic low-grade inflammation,remains largely *** address this question,we studied the levels of six inflammatory cytokines as potential biomarkers of a low-grade inflammatory state in elite athletes after an 11-month train-ing and competition *** We collected sera from 27 Finnish elite cross-country skiers and 27 gender-and age-matched,moderately-exer-cising *** serum concentrations of C-reactive protein(CRP),interleukin 6(IL-6),tumor necrosis factor alpha(TNF-α),glycoprotein acetyls(GlycA),interleukin 10(IL-10),and interferon gamma(IFN-γ)cytokines were quantified as surrogate markers of low-grade *** The athletes were found to have significantly lower concentrations of CRP(P=0.0232)and higher concentrations of IL-10,TNF-α,and IFN-γ(P=0.0097,P=0.0256,and P=0.0185,respectively)than the *** significant differences between athletes and controls were detected in the concentrations of IL-6 and *** inflammatory score(IS)did not differ significantly between athletes and *** The results of this study argued against the hypothesis of a significant chronic low-grade inflammation in response to prolonged high-performance exercise among elite endurance athletes.

关键词： Athlete Biomarker Chronic low-grade inflammation Exercise GlycA Inflammation

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