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JA but not JA-Ile is the cell-nonautonomous signal activating JA mediated systemic defenses to herbivory in Nicotiana attenuata

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Journal of Integrative Plant Biology 2017年第8期59卷 552-571页

作者： Tobit A. Bozorov son truong Dinh Ian T. Baldwin Department of Molecular Ecology Max Planck Institute for Chemical EcologyHans-Knoll-Str.8D-07745JenaGermany Institute of Genetics and Plants Experimental Biology Uzbek Academy of SciencesYukori-Yuz111226KibrayTashkent RegionUzbekistan Department of Plant Biotechnology Faculty of Biotechnology-Vietnam National University of AgricultureNgo Xuan Quang Street100000HanoiVietnam

The whole-plant activation of defense responses to wounding and herbivory requires systemic signaling in which jasmonates （JAs） play a pivotal role. To examine the nature of the slower cell-nonautonomous as compared to the rapid cell-autonomous signal in mediating systemic defenses in Nicotiana attenuata, reciprocal stem graftingexperiments were used with plants silenced for the JA biosynthetic gene ALLENE OXIDE CYCLASE （irAOC） or plants transformed to create JA sinks by ectopicalIy expressing Arabidopsis JA-O-methyltransferase （ovJMT）. JA-impaired irAOC plants were defective in the cell-nonautonomous signaling pathway but not in JA transport. Conversely, ovJMT plants abrogated the production of a graft-transmissible JA signal. Both genotypes displayed unaltered cell-autonomous signaling. Defense responses （17-hydroxygeranyllinalool diterpene glycosides, nicotine, and proteinase inhibitors） and metabolite profiles were differently induced in irAOC and ovJMT scions in response to graft-transmissible signals from elicited wild type stocks. The performance of Manduca sexta larvae on the scions of different graft combinations was consistent with the patterns of systemic defense metabolite elicitations. Taken together, we conclude that JA and possibly MeJA, but not JA-lle, either directly functions as a long-distance transmissible signal or indirectly interacts with long distance signal（s） to activate systemic defense responses.

关键词： COYOTE tobacco JASMONATE GENOTYPE METABOLITES ALLENE oxide cyclase

First Records of Megophrys daweimontis Rao and Yang, 1997 and Amolops vitreus(Bain, Stuart and Orlov, 2006)(Anura: Megophryidae, Ranidae) from Vietnam

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Asian Herpetological Research 2015年第1期6卷 66-72页

作者： Dzung Trung LE Anh Van PHAM son Hung Lan NGUYEN Thomas ZIEGLER truong Quang NGUYEN Faculty of Biology Hanoi National University of Education Faculty of Biology and Chemistry Tay Bac University AG Zoologischer Garten Kln Institute of Ecology and Biological Resources Vietnam Academy of Science and Technology

Two amphibian species,Megophrys daweimontisandAmolops vitreus,are recorded for the ifrst time from Vietnam, on the basis of a new amphibian collection from Dien Bien and son La provinces. In addition, acoustic analysis ofA. vitreus is also provided based on the advertisement calls recorded in Muong Nhe Nature Reserve of Dien Bien Province, Vietnam.

关键词： Amolops vitreus Megophrys daweimontis new records advertisement call Dien Bien Province son La Province

DICER-like Proteins and Their Role in Plant-herbivore Interactions in Nicotiana attenuata

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Journal of Integrative Plant Biology 2012年第3期54卷 189-206页

作者： Tohir Ahmadovich Bozorov Shree Prakash Pandey son truong Dinh Sang-Gyu Kim Maria Heinrich Klaus Gase Ian T. Baldwin Department of Molecular Ecology Max Planck Institute for Chemical Ecology 07745 Jena Germany Department of Biological Sciences Indian Institute of Science Education and Research Kolkata Mohanpur CampusPO: BCKV Campus Main Office Mohanpur Nadia West Bengal 741252 India.

DICER-like （DCL） proteins produce small RNAs that silence genes involved in development and defenses against viruses and pathogens. Which DCLs participate in plant-herbivore interactions remains unstudied. We identified and stably silenced four distinct DCL genes by RNAi in Nicotiana attenuata （Torrey ex. Watson）, a model for the study of plant-herbivore interactions. Silencing DCL1 expression was lethal. Manduca sexta larvae performed significantly better on ir-dcl3 and ir-dcl4 plants, but not on ir-dcl2 plants compared to wild type plants. Phytohormones, defense metabolites and microarray analyses revealed that when DCL3 and DCL4 were silenced separately, herbivore resistance traits were regulated in distinctly different ways. Crossing of the lines revealed complex interactions in the patterns of regulation. Single ir-dcl4 and double ir-dcl2 ir-dcl3 plants were impaired in JA accumulation, while JA- Ile was increased in ir-dcl3 plants. Ir-dcl3 and ir-dcl4 plants were impaired in nicotine accumulation; silencing DCL2 in combination with either DCL3 or DCL4 restored nicotine levels to those of WT. Trypsin proteinase inhibitor activity and transcripts were only silenced in ir-dcl3 plants. We conclude that DCL2/3/4 interact in a complex manner to regulate anti-herbivore defenses and that these interactions significantly complicate the already challenging task of understanding smRNA function in the regulation of biotic interactions.

关键词： DICER-like proteins anti-herbivore defense phytohormone signaling Manduca sexta Nicotiana attenuata.

Effectiveness on post-stroke hemiplegia in patients:electroacupuncture plus cycling vs electroacupuncture alone

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Journal of Traditional Chinese Medicine 2023年第2期43卷 352-358页

作者： Minh Duc Nguyen Thanh Van Tran Quoc Vinh Nguyen Ninh Khac Nguyen son truong Vu Luu Trong Nguyen Linh Vu Phuong Dang Senior Specialist Clinic National Hospital of AcupunctureHanoi 1000Vietnam Department of Senior Specialist National Hospital of Acupuncture Hanoi 1000Vietnam Department of Senior Specialist Military Institute of Traditional MedicineHanoi 1000Vietnam Department of Training and Science National Hospital of AcupunctureHanoi 1000Vietnam Department of Senior Specialist National Hospital of AcupunctureHanoi 1000Vietnam Department of Rehabilitation 108 Military Central HospitalHanoi 1000Vietnam Department of Medicine Dai Nam UniversityHanoi 1000Vietnam

OBJECTIVE:To evaluate the effectiveness of cycling in combination with electroacupuncture in treatment of poststroke hemiplegia patients at National Hospital of Acupuncture,***:The study was designed as a single-centre,outcome-assessor-blinded parallel randomised controlled trial with 120 post-stroke hemiplegia patients randomly assigned into two groups:electroacupuncture plus cycling(CT group) and electroacupuncture(AT group).Patients were assessed before and after the treatment(using muscle grading,modified Rankin,Barthel,Orgorozo scores and electromyography).Statistical Man–Whitney U test,and Fisher’s exact tests were used to compare between CT and AT ***:The results reported statistically significant improvement in motor function in patients suffering from hemiplegia following ischemic stroke in both CT and AT *** in CT group experienced a greater improvement compared to those in AT group including better muscle contraction(increased frequency and amplitude of electromyography and increased muscle grading scale);increased recovery(Orgogozo scale),increased independency(Barthel scale) and decreased disability(Modified Rankin scale)(P < 0.01).CONCLUSIONS:Combination with cycling training significantly improves the recovery of post-stroke patients treated with electroacupuncture.

关键词： electroacupuncture cycle training post-stroke hemiplegia

Embryonic, genetic and clinical outcomes of fresh versus vitrified oocyte: A retrospective cohort study

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Asian pacific Journal of Reproduction 2023年第6期12卷 266-272页

作者： Phuong Dao Thi son Dang truong Thuan Nguyen Duc Anh Pham Van Anh Do Tuan Van Hanh Nguyen HP Fertility-Hai Phong International Hospital of Obstetrics and Pediatrics Haiphong180000Vietnam Graduate University of Science and Technology Vietnam Academy of Science and TechnologyHanoi100000Vietnam Institute of Biotechnology Vietnam Academy of Science and TechnologyHanoi100000Vietnam

Objective:To compare embryonic development,ploidy status and clinical outcomes between fresh and frozen-thawed ***:This retrospective cohort study evaluated 83 fertilization cycles including both fresh and frozen oocytes from 79 patients at the HP Fertility Center of Hai Phong International Hospital of Obstetrics and Pediatrics in *** patient underwent several ovarian stimulation cycles to accumulate a certain number of oocytes that would be *** the last oocyte retrieval,all patient’s oocytes including both frozen and fresh would be *** outcomes included the rates of oocyte survival,cleavage embryo,blastocyst,ploidy status,pregnancy,biochemical pregnancy and clinical ***:The oocyte survival rate after thawing was 96.5%.No statistically significant difference was found when comparing fresh and frozen oocytes regarding fertilization rate(78.1%vs.75.5%,P=0.461),usable cleavage embryo rate(86.9%vs.87.2%,P=0.916)but usable blastocyst rate was found higher statistically in the frozen oocyte group(44.4%vs.54.0%,P=0.049).The percentages of euploid,aneuploid and mosaic embryos between the fresh group and the vitrified group had no significant differences(33.8%vs.31.6%,P=0.682;51.0%vs.54.2%,P=0.569;15.2%vs.12.4%,P=0.787;respectively).The rates of pregnancy,biochemical pregnancy and clinical pregnancy had no statistical difference(68.8%vs.64.8%,P=0.764;12.5%vs.3.6%,P=0.258;37.5%vs.46.4%,P=0.565).17 Mature oocytes are the minimum to have at least one euploid ***:Oocyte vitrification does not affect embryonic,genetic and clinical *** number of mature oocytes should be considered for fertilization in some cases.

关键词： Clinical outcomes Embryonic development Genetic outcomes Human oocyte Oocyte accumulation Oocyte cryopreservation Ploidy status Vitrified oocyte

Flow Assurance in Subsea Pipeline Design for Transportation of Petroleum Products

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Open Journal of Civil Engineering 2017年第2期7卷 311-323页

作者： son Tung Pham Minh Huy truong Ba Tuan Pham Faculty of Geology & Petroleum Engineering Hochiminh City University of Technology-Vietnam National University Hochiminh Vietnam

Transportation of petroleum products through pipeline presents considerable risks including wax formation and deposition as a result of heat loss of fluids, which is harmful to the flow due to the reduced inner diameter or totally blocked pipelines in extreme cases. The production interruption due to blocked pipelines can cause colossal financial loss. Therefore, in order to diminish those adverse effects, it is critical that pipeline design for flow assurance should be considered. Flow assurance is a relatively new field in oil and gas industry, it means that the flow of hydrocarbon stream from one point to another must be ensured successfully and economically. Although flow assurance is extremely diverse, encompassing many discrete and specialized subjects and bridging across the full gamut of engineering disciplines, our work concentrated principally on the study of wax deposit in the pipelines. The main purpose of this paper is to focus on the aspect of material in pipeline design and the selection of thermal insulation coatings. Furthermore, operating parameters such as pressure, temperature and flowrate will be examined to achieve optimum results. For the case study in this paper, the pipeline connecting Ca NguVang Oilfield’s Wellhead Platform (WHP) to the Central Processing Platform of Bach Ho Oilfield (CPP-3) in Vietnam will be studied. Hence, this work covers several aspects, namely the theoretical study, the modeling using Excel as well as using specialized software OLGA, and finally the application for a real case in the petroleum industry in Vietnam.

关键词： Flow Assurance Pipeline Design Thermal Insulation Wax Deposition Heat Transfer in Pipeline

Detecting Chlorophyll a Concentration and Bloom Patterns at Upwelling Area in South Central Vietnam by High Resolution Multi-satellite Data

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Journal of Environmental Science and Engineering(A) 2015年第5期4卷 215-224页

作者： Tong Phuoc Hoang son truong Minh Chuan Hoang Cong Tin Institute of Oceanography Vietnam Academy Sciences and Technology Nha Trang 8458 Vietnam Department of Education and Administration Kien Giang University Kien Giang 8477 Vietnam Department of Environment and Agriculture Curtin University WA 6102 Australia

Study on Chlorophyll a （Chl. a） distribution and bloom patterns is essential in the upwelling areas that constitute the main fishery grounds of Vietnam. Based on high resolution satellite imagery and monthly in-situ data in period from 2007 to 2008, the spatial and temporal variations ofChl, a in the upwelling region including algal blooms have been detected. Anomalies of higher Chl. a concentration and bloom patterns occurred at different periods, locations, and bloom shapes in coastal waters of Southern Center of Vietnam. The appearances of these bloom are related to four difference reasons, such as：（i） Their locations coincided to main center of upwelling phenomena that usually occur during southwest monsoon （SWM）; （ii） Effect of northeast monsoon （NEM） that brings and attaches the nutrient into the coast and created ＂floating＂ algae bloom patches in coastal zones; （iii） The algae blooms can be potentially associated with Harmful Algae Bloom （HAB） during the SWM or local eddies during NEM and （iv） Their appearances are also as a result of distinctive nutrient inflow fi＇om Mekong delta and pumping of nutrients by internal waves in coastal shallow waters.

关键词： Chlorophyll a bloom pattern fisheries resources coastal waters remote sensing upwelling.

EXISTENCE AND STABILITY OF PERIODIC AND ALMOST PERIODIC SOLUTIONS TO THE BOUSSINESQ SYSTEM IN UNBOUNDED DOMAINS

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Acta Mathematica Scientia 2022年第5期42卷 1875-1901页

作者： Thieu Huy NGUYEN truong Xuan PHAM Thi Ngoc Ha VU The Sac LE School of Applied Mathematics and Informatics Hanoi University of Science and Technology Vien Toan ung dung va Tin hocDai hoc Bach khoa Hanoi 1DaiCoVietHanoiVietnam Department of Mathematics Faculty of Information TechnologyThuyloi university Khoa Cong nghe Thong tinBo mon ToanDai hoc Thuy loi175 Tay SonDong DaHa NoiViet Nam Thuyloi University Dai hoc Thuy Loi175 Tay SonDong DaHanoiViet Nam

In this paper we investigate the existence and stability of periodic solutions(on a half-line R_(+))and almost periodic solutions on the whole line time-axis R to the Boussinesq system on several classes of unbounded domains of R^(n) in the framework of interpolation *** the linear Boussinesq system we combine the L^(p)—L^(q)-smoothing estimates and interpolation functors to prove the existence of bounded mild ***,we prove the existence of periodic solutions by invoking Massera’s *** also prove the existence of almost periodic *** we use the results of the linear Boussinesq system to establish the existence,uniqueness and stability of the small periodic and almost periodic solutions to the Boussinesq system using fixed point arguments and interpolation *** results cover and extend the previous ones obtained in[13,34,38].

关键词： Boussinesq systems periodic solutions almost periodic solutions unbounded domains smoothing estimates dual estimates interpolation spaces stability

软木塞基碳量子点的水热合成及其生物相融荧光成像应用

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新型炭材料 2022年第3期37卷 595-602页

作者： Quang Ngo Khoa Hieu Nguyen Ngoc Bao Vo Van Quoc Phuoc Vo Thi Ngoc Le Xuan Diem Doc Luong Quang Tri Nguyen Minh son Le Vu truong son Le Van Thanh Ha Che Thi Cam University of Sciences Hue University77 Nguyen HueHueVietnam Faculty of Environmental and Natural Sciences Duy Tan UniversityDa Nang550000Vietnam Institute for Research and Training in Medicine Biology and PharmacyDuy Tan UniversityDa Nang550000Vietnam University of Agriculture and Forestry Hue University102 Phung HungHueVietnam University of Science and Education The University of Da Nang459 Ton Duc ThangLien ChieuDa NangVietnam

以废弃酒瓶软木塞为原料,采用低成本、简单的水热方法合成碳量子点。通过TEM、FTIR、Raman、UV-Vis、PL光谱对碳量子点的结构和光学性能进行分析表征。结果表明,碳量子点的平均直径为6.2±2.7 nm,PL激发谱和碳量子点表面的官能团有关。... 详细信息

以废弃酒瓶软木塞为原料,采用低成本、简单的水热方法合成碳量子点。通过TEM、FTIR、Raman、UV-Vis、PL光谱对碳量子点的结构和光学性能进行分析表征。结果表明,碳量子点的平均直径为6.2±2.7 nm,PL激发谱和碳量子点表面的官能团有关。用硫酸奎宁作为参考,碳量子点的量子效率为1.54%。将获得的碳量子点应用在骨髓间充质干细胞的细胞生物成像上,发现用碳量子点处理后,骨髓间充质干细胞分别在320~380 nm、450~490 nm和450~490 nm范围显示绿色、黄色和红色荧光,表明了碳量子点在荧光成像领域具有潜在的应用价值。

关键词：碳点水热合成荧光荧光图像废酒瓶塞