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Characterization of two Achromobacter xylosoxidans isolates from patients with pertussis-like symptoms

Asian Pacific Journal of Tropical Medicine 2015年第6期8卷 465-468页

作者： Fiorella Orellana-Peralta Michelle Jacinto Maria J.Pons Cláudia Gomes Carlos Bada Isabel Reyes Juana del Valle Mendoza joaquim ruiz Facultad de Ciencias de la Salud.Universidad Peruana de Ciencias Aplicadas-LiPC LimaPeru ISGlobal Barcelona Ctr.Int.Health Res.(CRESIB)Hospital Clinic-Universitat de Barcelona Hospital de Emergencias Pediatricas.Lima Peru Instituto de Investigacion Nutrirional LimaPeru

Objective:To characterize two Achromobaeter xylosoxidans recovered from 2 patients diagnosed with pertussis during a Bordetella pertussis surveillance ***:Nasopharyngeal swabs from 2 children under 1 year of age with clinical suspicion of pertussis were analyzed by culture and ***:Two Achromobaeter xylosoxidans A8,closely related to Bordetella *** recovered from 2 patients diagnosed of pertussis,both carrying the ptxA gene and IS418 the pertussis toxin encoding ***,antibiotic susceptibility was evaluated by disk-diffusion method and by ***:Although more detailed studies are needed,the present data highlight the possibility that Achromobaeter *** related Bordetella pertussis microorganisms and not covered under the vaccine umbrella,might also result in cases of whooping *** further surveillance is necessary to determine the extension and relevance of their pathogenic role in order to discriminate their real public health implication.

关键词： Achromobacter xylosoxidans Bordetella pertussis

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Comparison of the patient and partner satisfaction with 700CX and Titan penile prostheses

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Asian Journal of Andrology 2017年第3期19卷 321-325页

作者： Javier Romero Otero Cesar Rojas Cruz Borja Garcia Gomez joaquim Sarquella Geli lose Medina Polo Eduard ruiz Castane Alfredo Rodriguez Antolin 12 de Octubre University Hospital Department of Urology Madrid Spain Fundacio Puigvert Department of Andrology Barcelona Spain.

Most widespread three-component penile prosthesis models are 700CXTM and Titan~. Our purpose is to assess patient and partner satisfaction after the first implant. This is a multicenter, retrospective, nonrandomized study in which all patients who met the inclusion criteria between 2009 and 2013 were included. In total, 248 patients agreed to participate. To evaluate patient satisfaction, a validated but modified 11-question questionnaire was completed （EDITS）; and a nonvalidated two-item questionnaire was given to the partner. Statistical analysis used an ordinal logistic regression model. Two hundred and forty-eight patients （194 with 700CXTM vs 54 with Titan） and 207 couples completed the questionnaire （165 with 700CXTM vs 42 with Titan）. Overall satisfaction was high. Both showed great reliability for sexual intercourse and high compliance with prior expectations. Most patients were able to manage the penile prosthesis correctly within 6 months. Postoperative penile shortening led to some dissatisfaction in 42% and 46% of cases （700CX/Titan）. Significant differences were found in three questions of patients＇ questionnaire. There were more patients satisfied with the 700CXTM （P= 0.0001）. No patient with Titan implant took longer than 6 months to optimal management. Only 4% of patients with 700CXTM implant were dissatisfied with the deflation, in contrast to 24% with the Titan （P = 0.0031）. Of the two partners＇ questions, one showed a statistically significant difference （P = 0.0026）. It seems that group 700CX＂ would recommend to re-implant the prosthesis with a greater tendency. The overall satisfaction was very high for both prostheses. The final aspect of the erected and flaccid penis was satisfactory, but both groups showed significant discontent with its final size. Partners＇ overall satisfaction was high.

关键词： erectile dysfunction penile implantation penile prosthesis

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Ten-year experience with penile prosthetic surgery for the treatment of erectile dysfunction:outcomes of a tertiary referral center and predictors of early prosthetic infection

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Asian Journal of Andrology 2022年第1期24卷 32-39页

作者： Franco Palmisano Luca Boeri Riccardo Ievoli Josvany Sánchez-Curbelo Matteo Giulio Spinelli Andrea Gregori Antonio Maria Granata Eduard ruiz-Castane Emanuele Montanari joaquim Sarquella-Geli ASST Fatebenefratelli-Sacco Department of UrologyLuigi Sacco University HospitalMilan 20157Italy IRCCS Foundation Ca’Granda Ospedale Maggiore Policlinico Department of UrologyUniversity of MilanMilan 20122Italy Department of Economics and Management University of FerraraFerrara 44121Italy FundacióPuigvert Department of AndrologyUniversity of BarcelonaBarcelona 08025Spain

We aimed to evaluate ten-year outcomes of penile prosthesis(PP)implantation for the treatment of erectile dysfunction and to assess predictors of early prosthetic infection(EPI).We identified 549 men who underwent 576 PP placements between 2008 and *** and multivariate analyses were used to identify potential predictors of *** EPI predictive nomogram was ***-five(6.1%)cases of EPI were recorded with an explant rate of 3.1%.In terms of satisfaction,82.0%of the patients defined themselves as“satisfied,”while partner’s satisfaction was 88.3%.Diabetes(P=0.012),longer operative time(P=0.032),and reinterventions(P=0.048)were associated with EPI risk,while postoperative ciprofloxacin was inversely associated with EPI(P=0.014).Rifampin/gentamicin-coated 3-piece inflatable PP(r/g-c 3IPP)showed a higher EPI risk(P=0.019).Multivariate analyses showed a two-fold higher risk of EPI in diabetic patients,redo surgeries,or when a r/g-c 3IPP was used(all P<0.03).We showed that diabetes,longer operative time,and secondary surgeries were the risk factors for *** ciprofloxacin was associated with a reduced risk of EPI,while r/g-c 3IPP had higher EPI rates without an increased risk of PP *** further validation,the proposed nomogram could be a useful tool for the preoperative counseling of PP implantation.

关键词： Boston Coloplast Titan three-piece penile prosthesis erectile dysfunction impotence Inhibizone patient satisfaction penile prosthesis prosthesis infection rifampin soaking

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Transient and DNA-free in vivo CRISPR/Cas9 genome editing for flexible modeling of endometrial carcinogenesis

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Cancer Communications 2023年第5期43卷 620-624页

作者： Raúl Navaridas Maria Vidal-Sabanés Anna ruiz-Mitjana Aida Perramon-Güell Cristina Megino-Luque David Llobet-Navas Xavier Matias-Guiu joaquim Egea Mario Encinas Lídia Bardia Julien Colombelli Xavier Dolcet Oncological Pathology Group Department of Basic Medical SciencesUniversity of LleidaBiomedical Research Institute of LleidaLleidaSpain Molecular Mechanisms and Experimental Therapy in Oncology-Oncobell Program Bellvitge Biomedical Research Institute’L’Hospitalet de LlobregatBarcelonaSpain Department of Pathology Bellvitge HospitalUniversity of BarcelonaBellvitge Biomedical Research InstituteL’Hospitalet de LlobregatBarcelonaSpain Department of Pathology Arnau de Vilanova HospitalUniversity of LleidaBiomedical Research Institute of LleidaLleidaSpain Cancer Networking Biomedical Research Center Institute of Health Carlos IIIMadridSpain Molecular Developmental Neurobiology Group Department of Basic Medical SciencesUniversity of LleidaBiomedical Research Institute of LleidaLleidaSpain Developmental and Oncogenic Signalling Group Department of ExperimentalMedicineUniversity of LleidaBiomedical Research Institute of LleidaLleidaSpain Advanced Digital Microscopy Core Facility Institute for Research in BiomedicineBarcelona Institute of Science and TechnologyBarcelonaSpain

Dear Editor,The Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR)and CRISPR-associated protein 9(Cas9)(CRISPR/Cas9)-mediated generation of somatically genetically engineered mouse models have emerged as a new approach for in vivo modeling of cancer[1].Here,we describe a novel DNA-free,easy,rapid,flexible,multiplexable,and robust method to model endometrial neoplasia by CRISPR/Cas9 ribonucleoprotein(RNP)electroporation into the uterus of mice.

关键词： CRISPR/Cas9 DNA CRISPR

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