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Serum outperforms plasma in small extracellular vesicle microRNA biomarker studies of adenocarcinoma of the esophagus

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World Journal of Gastroenterology 2020年第20期26卷 2570-2583页

作者： Karen chiam George c Mayne Tingting Wang David I Watson Tanya S Irvine Tim Bright Lorelle T Smith Imogen A Ball joanne M Bowen Dorothy M Keefe Sarah K Thompson Damian J Hussey Discipline of Surgery College of Medicine and Public HealthFlinders University of South AustraliaAdelaideSA 5042Australia Flinders Health and Medical Research Institute cancer Program College of Medicine and Public HealthFlinders UniversityAdelaideSA 5042Australia Discipline of Surgery The University of AdelaideAdelaideSA 5005Australia Adelaide Medical School The University of AdelaideAdelaideSA 5005Australia Faculty of Health Sciences The University of AdelaideAdelaideSA 5005Australia

BAcKGROUND circulating microRNAs(miRNAs)are potential biomarkers for many ***,they can originate from non-disease specific sources,such as blood cells,and compromise the investigations for miRNA *** small extracellular vesicles(sEVs)have been suggested to provide a purer source of circulating miRNAs for biomarkers discovery,the most suitable blood sample for sEV miRNA biomarker studies has not been *** To compare the mi RNA profiles between matched serum and plasma s EV preparations to determine their suitability for biomarker *** Matched serum and plasma samples were obtained from 10 healthy controls and10 patients with esophageal adenocarcinoma.s EV isolates were prepared from serum and plasma using Exo Quick TM and quantified using Nano *** was extracted from s EV preparations with the mi RNeasy Serum/Plasma kit and profiled using the Taqman Openarray q *** overall mi RNA content and theexpression of specific mi RNAs of reported vesicular and non-vesicular origins were compared between serum and plasma s EV *** diagnostic performance of a previously identified multi-mi RNA biomarker panel for esophageal adenocarcinoma was also *** The overall mi RNA content was higher in plasma s EV preparations(480 mi RNAs)and contained 97.5%of the mi RNAs found in the serum s EV preparations(412 mi RNAs).The expression of commonly expressed mi RNAs was highly correlated(Spearman’s R=0.87,Pconsistently higher in the plasma s EV *** blood-cell mi RNAs(hsa-mi R-223-3 p,hsa-mi R-451 a,mi R-19 b-3 p,hsa-mi R-17-5 p,hsa-mi R-30 b-5 p,hsa-mi R-106 a-5 p,hsa-mi R-150-5 p and hsa-mi R-92 a-3 p)were expressed at 2.7 to 9.6 fold higher levels in the plasma s EV preparations compared to serum s EV preparations(Pcentage of protein-associated mi RNAs expressed at relatively higher levels(ct

关键词： Biomarkers Exosomes Extracellular vesicles circulating microRNA MicroRNAs Plasma Serum Blood cells Real-time polymerase chain reaction Adenocarcinoma of esophagus

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ZMyM3:a new candidate gene in nonobstructive azoospermia?

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Asian Journal of Andrology 2023年第5期25卷 648-649页

作者： Morgane Le Beulze Nelly Swierkowski-Blanchardl Farah Ghieh joanne Fortemps carole Gerault Valerie Scrazin Anne Louboutin-Sanchez Marc Bailly Francois Vialard Paris-Saclay University UVSQINRAEBREEDJouy-en-Josas F-78350France Alfort National Veterinary Scholl BREEDMaisons Alfort F-94700France Department of Gynecology and Obstetric CHI de Poissy/Saint-Germain-en-LayePoissy F-78300France Unit of Anatomo-Pathology CHI de Poissy/Saint-Germain-en-LayePoissy F-78300France Department of Genetic CHI de Poissy/Saint-Germain-en-LayePoissy F-78300France

Dear Editor,Infertility affects about 15%of couples of childbearing age.i About half of these cases can be attributed predominantly to a male factor,such as a quantitative or qualitative impairment in *** etiology of male infertility is related to anatomic,genetic,and environmental causes,being sometimes multifactorial and of unknown or idiopathic *** present,genetic screening for nonobstructive azoospermia(NOA)is generally limited to karyotyping for the identification of chromosome abnormalities such as 47,XXy,46,X,der(X)t(X;y)(p22.3;p11.2),chromosome rearrangements,and y chromosome ***-genome analyses have shown that over 100 gene variants are associated with a male infertility phenotype,and the list continues to grow;hence,a large number of genes are involved in *** continuing identification of genes responsible for male infertility in general and azoospermia,in particular,is a major challenge in both research and the diagnosis ofmale infertility.

关键词： azoospermia obstructive diagnosis

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Short-and long-term outcomes after hemihepatectomy for perihilar cholangiocarcinoma:does left or right side matter?

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Hepatobiliary Surgery and Nutrition 2021年第2期10卷 154-162,I0001,I0002页

作者： Lotte c.Franken Pim B.Olthof Joris I.Erdmann Otto M.van Delden joanne Verheij Marc G.Besselink Olivier R.Busch Thomas M.van Gulik Department of Surgery Cancer Center AmsterdamAmsterdam UMCUniversity of AmsterdamThe Netherlands Department of Surgery Erasmus Medical CenterErasmus UniversityRotterdamThe Netherlands Department of Radiology and Nuclear Medicine Amsterdam UMCUniversity of AmsterdamAmsterdamThe Netherlands Department of Pathology Cancer Center AmsterdamAmsterdam UMCUniversity of AmsterdamAmsterdamThe Netherlands

Background:The only potentially curative option for patients with perihilar cholangiocarcinoma(PHc)is resection,typically an extrahepatic bile duct resection in combination with(extended)liver *** such as bile leakage and liver failure have been suggested to be more common after right-sided resections compared to left-sided resections,whilst superior oncological outcomes have been reported after right-sided ***,data on outcomes after right-sided or left-sided liver resections in PHc are ***,we aimed to investigate short-and long-term outcomes after left and right hemihepatectomy in patients with ***:In this retrospective study,patients undergoing major liver resection for suspected PHc in a tertiary center between 2000-2018 were *** who had undergone left-sided resections were compared to patients with right-sided resections in terms of complications(90-day mortality,overall and severe morbidity and specific complications).For long-term outcomes,only patients with pathologically proven PHc were included in the survival ***:A total of 178 patients undergoing hemihepatectomy for suspected PHc were analysed,including 76 left-sided and 102 right-sided *** 90-day mortality was 14%(24 out of 178),with no significant difference after left-sided resection(11%;8 out of 76)versus right-sided resection(16%;16 out of 102)(P=0.319).Severe morbidity(clavein Dindo≥3)was also comparable in both groups:54%versus 61%(P=0.361).No differences in specific complications including bile leakage were observed,although liver failure appeared to occur more frequently after right hemihepatectomy(22%versus 11%,P=0.052).Five-year overall survival for pathologically proven PHc,excluding in-hospital mortality,did not differ;43.7%after left-sided resection *** 38.2%after right-sided resection(P=0.553).conclusions:Both short-and long-term outcomes between patients undergoing left and right hemihepate

关键词： Perihilar cholangiocarcinoma(PHc) hepatectomy outcomes mortality survival

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Microfungi on Tectona grandis(teak)in Northern Thailand

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Fungal Diversity 2017年第1期82卷 107-182页

作者： Mingkwan Doilom Asha J.Dissanayake Dhanushka N.Wanasinghe Saranyaphat Boonmee Jian-Kui Liu D.Jayarama Bhat joanne E.Taylor Ali.H.Bahkali Eric H.c.McKenzie Kevin D.Hyde Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of Sciences132 Lanhei RoadKunming 650201China center of Excellence in Fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailand School of Science Mae Fah Luang UniversityChiang Rai 57100Thailand Institute of Plant and Environment Protection Beijing Academy of Agriculture and Forestry SciencesNo.9 of Shu Guang Hua Yuan Zhong LuHaidian DistrictBeijing 10097China Guizhou Key Laboratory of Agricultural Biotechnology Guizhou Academy of Agricultural SciencesGuiyang 550006GuizhouChina Formerly Department of Botany Goa UniversityTaleigãoGoaIndia No.128/1-J Azad Housing SocietyCurcaGoa VelhaIndia Royal Botanic Garden Edinburgh 20A Inverleith RowEdinburgh EH35LRUK Department of Botany and Microbiology College of ScienceKing Saud UniversityP.O.Box 2455Riyadh 1145Saudi Arabia Manaaki Whenua Landcare Research Private Bag 92170AucklandNew Zealand

To date there is virtually no information available concerning the fungi associated with Tectona grandis(teak)(Lamiaceae)in *** this study,samples of microfungi were collected from both asymptomatic stems and dead wood,and symptomatic branches,stem and leaves of *** 27 sites in six provinces(chiang Mai,chiang Rai,Phayao,Phitsanulok,Phrae and Uttaradit Provinces).Morphology and combined multi-gene phylogeny(cAL,GAPDH,ITS,LSU,RPB2,SSU,TEF1 and TUB)were used to identify taxa.A total of 270 collections,representing 28 fungal species residing in 12 families,7 orders and 21 genera,with three species of uncertain taxonomic placement were *** these,one family,three genera and 14 species are new to *** new family,Pseudocoleodictyosporaceae is introduced based on its distinct lineage in the Dothideomycetes and its unique morphology as compared to Roussoellaceae and *** new genera are Neooccultibambusa,Pseudocoleodictyospora and *** newly described species are Diaporthe neoraonikayaporum,***,***,***,Hermatomyces tectonae,***,Manoharachariella tectonae,Neooccultibambusa chiangraiensis,Pseudocoleodictyospora sukhothaiensis,***,***,Rhytidhysteron tectonae,Subglobosporium tectonae and Tubeufia *** species are known published taxa including Alternaria tillandsiae,Berkleasmium talaumae,Boerlagiomyces macrospora,ceratocladium purpureogriseum,Fusarium solani,Helicoma siamense,Lasiodiplodia theobromae,Macrovalsaria megalospora,Paradictyoarthrinium diffractum,Phaeoacremonium italicum,Sphaeropsis eucalypticola,Stachybotrys levispora,*** and Thaxteriellopsis *** or reference specimens are designated for Boerlagiomyces macrospora and Macrovalsaria *** megalospora is transferred from Botryosphaeriaceae to Dothideomycetes genus,incertae sedis based on taxonomy and phylogenetic analysis,which indicate i

关键词： Ascomycota Basidiomycota Dothideomycetes Sordariomycetes Taxonomy Multi-gene phylogenetics

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疼痛对乳腺癌患者生活质量影响调查

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中国循证医学杂志 2008年第4期8卷 233-236页

作者：王世平马玲 joanne chung 四川大学华西护理学院成都610041 香港理工大学护理学院

目的了解乳腺癌患者的疼痛感受和生活质量状况,评估疼痛对生活质量的影响程度。方法采用中国人癌症疼痛评估工具(chinese cancer Pain Assessment Tool,ccPAT)和生活质量调查问卷(第3版)[EORTIc QLQ-c30(Version 3)]对200例乳腺癌患者... 详细信息

目的了解乳腺癌患者的疼痛感受和生活质量状况,评估疼痛对生活质量的影响程度。方法采用中国人癌症疼痛评估工具(chinese cancer Pain Assessment Tool,ccPAT)和生活质量调查问卷(第3版)[EORTIc QLQ-c30(Version 3)]对200例乳腺癌患者进行横断面设计的面对面问卷调查。结果200例乳腺癌患者中有疼痛者84例(42%),无疼痛者116例(58%)。疼痛组患者疼痛评分高于非疼痛组患者评分(P0.05);疼痛组患者的疼痛评分与整体生活质量评分呈显著负相关(r=–0.731,P<0.001)。结论疼痛对乳腺癌患者的躯体功能、角色功能和社会功能有一定的负面影响;患者受疼痛的冲击加重时,其整体生活质量降低。

关键词：乳腺癌疼痛生活质量横断面调查

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Auxetic Plate with a crack under Shear Loading

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材料科学与工程（中英文B版） 2011年第5期1卷 565-574页

作者： Sukhwinder Kaur Bhullar joanne Wegner Andrew Mioduchowski Department of Mechanical Engineering University of Victoria Victoria B. C. V8W 3P6 Canada Department of Mechanical Engineering University of Alberta Edmonton AB T6G 2G8 Canada

Auxetic materials have special and desirable mechanical properties. For example, if the material has a crack, when it is being pulled apart, it expands and closes up the crack. A mathematical model of an infinite plate with a line crack subjected to the mechanical and thermal loading is developed to compare crack resistance to fracture of auxetic material with non auxetic material. The results are obtained for both insulating and uninsulating cracks using complex function method in the context of thermoelastic theory. Polyurethane foam (PU) is the material chosen for numerical calculations and results are presented in the graphical form.

关键词：载荷作用裂缝剪切板带复变函数方法热弹性理论聚氨酯泡沫力学性能

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Regulation of proliferation by Kca3.1 channels is independent of channel electrophysiological function

Regulation of proliferation by KCa3.1 channels is independen...

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2008北京国际生理科学学术大会

作者： MILLERSHIP joanne BRUcE Jason I E DEVOR Daniel c FEARON Ian M Life Sciences the University of ManchesterManchester M13 9NTUK Department of cell Biology and Physiology School of MedicineUniversity of PittsburghPittsburghPA 15261USA

Potassium channels regulate proliferation of a number of cell *** the potassium channel family, K3.1(IK1/SK4),is involved in the proliferation of endothelial cells(Ec),T lymphocytes and several cancer cell *** respect to endothelial cells,these cells express a wide variety of ion channel types and subtypes,and the patterns of expression differ due to differences in vascular beds from which cells were isolated,passage number and culture *** this reason,it is difficult to dissect,and examine in isolation,the functional role of a specific potassium channel in Ec ***,to investigate the mechanisms underlying the role of K3.1 in cell proliferation in isolation,we performed proliferation assays in HEK293 cells expressing recombinant hK3.1 *** cells stably expressing this channel,selective inhibition of hK3.1 with 10μmol/L TRAM-34 reduced proliferation,while transient expression of hK3.1 in WT HEK293 cells increased *** mechanism by which hK3.1 stimulates cell proliferation is thought to be mediated by cell hyperpolarisation(via K efflux),which increases[ca] by driving ca influx through non-selective cation ***,when WT HEK293 cells were transiently transfected with a mutant(GyG/AAA)hK3.1 channel,which neither conducts K ions nor promotes ca entry,proliferation was increased relative to control(mock-transfected)***,when WT HEK293 cells were transiently transfected with a trafficking mutant(L18A/L25A)hK3.1 channel,which is not expressed at the cell surface,proliferation was also increased relative to control ***,both K efflux and ca influx are not necessary for hK 3.1-induced proliferation in HEK293 cells,and nor does hK 3.1 need to be expressed at the cell *** propose that hK 3.1-induced cell proliferation is independent of electrophysiological function and arises potentially due to direct regulation of intracellular signalling *** support of this hypothesis,in cells

关键词： Kca3.1 endothelial cells proliferation MAP kinase

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Occurrence of seeding metastases in resectable perihilar cholangiocarcinoma and the role of low-dose radiotherapy to prevent this

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World Journal of Hepatology 2020年第11期12卷 1089-1097页

作者： Lotte c Franken Eva Roos Job Saris Jeanin E van Hooft Otto M van Delden joanne Verheij Joris I Erdmann Marc G Besselink Olivier R Busch Geertjan van Tienhoven Thomas M van Gulik Department of Surgery Cancer Center AmsterdamAmsterdam UMCUniversity of AmsterdamAmsterdam 1105 AZNetherlands Department of Pathology Amsterdam UMCUniversity of AmsterdamAmsterdam 1105 AZNetherlands Department of Gastroenterology Amsterdam UMCUniversity of AmsterdamAmsterdam 1105 AZNetherlands Department of Interventional Radiology Amsterdam UMCUniversity of AmsterdamAmsterdam 1105 AZNetherlands Department of Radiation Oncology Amsterdam UMCUniversity of AmsterdamAmsterdam 1105 AZNetherlands

BAcKGROUND Preoperative biliary drainage in patients with presumed resectable perihilar cholangiocarcinoma(PHc)is hypothesized to promote the occurrence of seeding *** metastases can occur at the surgical scars or at the site of postoperative drains,and in case of percutaneous biliary drainage,at the catheter *** prevent seeding metastases after resection,we routinely treated PHc patients with preoperative radiotherapy(RT)for over 25 years until January *** To investigate the incidence of seeding metastases following resection of *** All patients who underwent resection for pathology proven PHc between January 2000 and March 2019 were included in this retrospective *** 2000-January 2018,patients received preoperative RT(3×3.5 Gray).RT was omitted in patients treated after January *** A total of 171 patients underwent resection for PHc between January 2000 and March *** 171 patients undergoing resection,111 patients(65%)were treated with preoperative *** bile cytology showed no difference in the presence of viable tumor cells in bile of patients undergoing preoperative RT or ***,two patients(1.2%)with seeding metastases were identified,both in the laparotomy scar and both after preoperative RT(one patient with endoscopic and the other with percutaneous and endoscopic biliary drainage).cONcLUSION The incidence of seeding metastases in patients with resected PHc in our series was low(1.2%).This low incidence and the inability of providing evidence that preoperative low-dose RT prevents seeding metastases,has led us to discontinue preoperative RT in patients with resectable PHc in our center.

关键词： Perihilar cholangiocarcinoma Seeding metastases Preventive radiotherapy

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果蝇以性信息素防范“不忠”

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昆虫知识 2009年第5期46卷 663-663页

作者： joanne y.yew

生物学家发现，在配对之前雌性果蝇对雄性果蝇有巨大魅力，可一旦它们结合后，雌果蝇就会失去对其他雄果蝇的吸引力，其原因一直不明。一个德美联合研究小组最近发现，是一种性信息素的作用导致了这一奇特现象。

关键词：性信息素果蝇防范生物学家吸引力雄性雌性

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新型去甲斑蝥素衍生物的设计合成及体外抗肿瘤活性

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第二军医大学学报 2008年第12期29卷 1470-1474页

作者：董环文刘超美李科 Lurong Zhang joanne Kim 第二军医大学药学院有机化学教研室上海200433 Department of Radiation Oneology URMC 601 Elmwood Ave. University of Rochester Rochester NY. USA. 14642

目的:设计合成新型去甲斑蝥素衍生物,并研究其体外抗肿瘤活性。方法:以呋喃和马来酸酐为起始原料经多步反应合成去甲斑蝥素衍生物,化合物经1HNMR、ESI-MS和元素分析确证结构;用MTT法,首先对所有化合物进行抗乳腺癌细胞株4T1体外活性筛选... 详细信息

目的:设计合成新型去甲斑蝥素衍生物,并研究其体外抗肿瘤活性。方法:以呋喃和马来酸酐为起始原料经多步反应合成去甲斑蝥素衍生物,化合物经1HNMR、ESI-MS和元素分析确证结构;用MTT法,首先对所有化合物进行抗乳腺癌细胞株4T1体外活性筛选,然后对活性较好的化合物7a和7c进一步作抗肺癌细胞株LLc和抗肝癌细胞株SK-HEP-1的活性检测,同时对这2种化合物进行对正常血管内皮细胞ABAE的损伤试验。结果:设计合成了18种新化合物。其中化合物7a和7c对肿瘤细胞株有一定的抑制作用,而对正常细胞的损伤程度较低。结论:化合物7a和7c对乳腺癌细胞株4T1、肺癌细胞株LLc和肝癌细胞株SK-HEP-1有一定的抑制作用,尤其是化合物7c对正常内皮细胞ABAE的损伤比对照药去甲斑蝥素小得多,有进一步研究的价值。

关键词：去甲斑蝥素合成抗肿瘤药

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