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Biofunctionalized all-polymer photonic lab on a chip with integrated solid-state light emitter

Light(Science & Applications) 2015年第1期4卷 430-437页

作者： Andreu Llobera joan Juvert Alfredo Gonzalez-Fernandez Bergoi Ibarlucea Ester Carregal-romero Stephanus Buttgenbach Cesar Fernandez-Sanchez Instituto de Microelectronica de Barcelona IMB-CNM(CSIC) Campus UAB08193 BellaterraSpain Institut fur Mikrotechnik Technische Universitat BraunschweigBraunschweig 38106Germany

A photonic lab on a chip(PhLOC),comprising a solid-state light emitter(SSLE)aligned with a biofunctionalized optofluidic multiple internal reflection(mir)system,is *** SSLE is obtained by filling a microfluidic structure with a phenyltrimethoxysilane(PhTMOS)aqueous sol solution containing a fluorophore organic *** curing,the resulting xerogel solid structure retains the emitting properties of the fluorophore,which is evenly distributed in the xerogel *** studies demonstrate that after a total dose(at λ5365 nm)greater than 24 J cm^(-2),the xerogel emission decay is only 4.1%.To re-direct the emitted light,the SSLE includes two sets of air mirrors that surround the *** mapping of the SSLE demonstrates that alignment variations of 150 mm(between the SSLE and the external pumping light source)provide fluctuations in emitted light smaller than 5%.After this verification,the SSLE is monolithically implemented with a mir,forming the *** performance is assessed by measuring quinolone yellow,obtaining a limit of detection(LOD)of(0.6060.01)***,the mir is selectively biofunctionalized with horseradish peroxidase(HrP)for the detection of hydrogen peroxide(H_(2)O_(2))target analyte,obtaining a LOD of(0.760.1)μM for H_(2)O_(2),confirming,for the first time,that solid-state xerogel-based emitters can be massively implemented in biofunctionalized PhLOCs.

关键词： medical devices photonic lab on a chip polymer technology solid-state light emitters sol–gel

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Co-expression network analysis predicts a key role of microrNAs in the adaptation of the porcine skeletal muscle to nutrient supply

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Journal of Animal Science and Biotechnology 2020年第2期11卷 327-347页

作者： Emilio Mármol-Sánchez Yuliaxis ramayo-Caldas raquel Quintanilla TainãFigueiredo Cardoso rayner González-Prendes joan Tibau Marcel Amills Centre for research in Agricultural Genomics(CrAG) CSIC-IRTA-UAB-UBUniversitat Autònoma de Barcelona08193 BellaterraSpain Animal Breeding and Genetics Program Institute for Research and Technology in Food and Agriculture(IRTA)Torre Marimon08140 Caldes de MontbuiSpain Present address:Embrapa Pecuária Sudeste Empresa Brasileira de Pesquisa Agropecuária(EMBRAPA)São CarlosSP 13560-970Brazil Department of Animal Science Universitat de Lleida-Agrotecnio Center25198 LleidaSpain Departament de Ciència Animal i dels Aliments Universitat Autònoma de Barcelona08193 BellaterraBarcelonaSpain

Background:The role of non-coding rNAs in the porcine muscle metabolism is poorly understood,with few studies investigating their expression patterns in response to nutrient ***,we aimed to investigate the changes in microrNAs(mirNAs),long intergenic non-coding rNAs(lincrNAs)and mrNAs muscle expression before and after food ***:We measured the mirNA,lincrNA and mrNA expression levels in the gluteus medius muscle of 12 gilts in a fasting condition(AL-T0)and 24 gilts fed ad libitum during either 5 h.(AL-T1,N=12)or 7 h.(AL-T2,N=12)prior to *** small rNA fraction was extracted from muscle samples retrieved from the 36 gilts and sequenced,whereas lincrNA and mrNA expression data were already *** terms of mean and variance,the expression profiles of mirNAs and lincrNAs in the porcine muscle were quite different than those of *** intake induced the differential expression of 149(AL-T0/AL-T1)and 435(AL-T0/AL-T2)mrNAs,6(AL-T0/AL-T1)and 28(AL-T0/AL-T2)mirNAs and none lincrNAs,while the number of differentially dispersed genes was much *** the set of differentially expressed mirNAs,we identified ssc-mir-148a-3p,ssc-mir-22-3p and ssc-mir-1,which play key roles in the regulation of glucose and lipid ***,co-expression network analyses revealed several mirNAs that putatively interact with mrNAs playing key metabolic roles and that also showed differential expression before and after *** case example was represented by seven mirNAs(ssc-mir-148a-3p,ssc-mir-151-3p,ssc-mir-30a-3p,ssc-mir-30e-3p,ssc-mir-421-5p,ssc-mir-493-5p and ssc-mir-503)which putatively interact with the PDK4 mrNA,one of the master regulators of glucose utilization and fatty acid ***:As a whole,our results evidence that microrNAs are likely to play an important role in the porcine skeletal muscle metabolic adaptation to nutrient availability.

关键词： Co-expression analysis lincrNAs microrNAs Pig regulatory impact factor Skeletal muscle

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Inhaler Technique Questionnaire(InTeQ)in pediatric patients with asthma

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World Journal of Pediatrics 2023年第8期19卷 798-804页

作者： Catalina Lizano‑Barrantes Olatz Garin Alexandra Lelia Dima Karina Mayoral Angels Pont Eva María Ortiz María Araceli Caballero‑rabasco Manuel Praena‑Crespo Laura Valdesoiro‑Navarrete María Teresa Guerra Alberto Bercedo‑Sanz Gimena Hernández Camila Maroni Inés de mir MaríaÁngeles Carrasco Marta Ortega Alberto Servan JoséAntonio Castillo Eva Tato Montse Ferrer Health Services research Group IMIM(Hospital del Mar Medical Research Institute)Barcelona Biomedical Research ParkDoctor Aiguader8808003 BarcelonaSpain Department of Medicine and Life Sciences Universitat Pompeu FabraBarcelonaSpain Department of Pharmaceutical Care and Clinical Pharmacy Faculty of PharmacyUniversidad de Costa RicaSan JoseCosta Rica Centro de Investigación Biomédica en red de Epidemiología y Salud Pública CIBErESP MadridSpain research and Development Unit Institut de Recerca Sant Joan de DéuBarcelonaSpain Department of Pediatrics Obstetrics and Gynaecology and Preventive MedicineUniversitat Autònoma de BarcelonaBarcelonaSpain Pediatric Allergy and Pulmonology Unit Pediatric ServiceHospital del MarBarcelonaSpain Pediatric Allergy and Pulmonology Unit Pediatric ServiceHospital Universitari Parc TaulíSabadellSpain Centro de Salud La Candelaria Servicio Andaluz de SaludSevilleSpain Grupo de Vías respiratorias de La Asociación Española de Pediatras de Atención Primaria(AEPAP) MadridSpain Centro de Salud Jerez Sur Servicio Andaluz de SaludSevilleSpain Centro de Salud La Sagrera BarcelonaSpain Centro de Salud Los Castros CantabriaSpain Facultat de Medicina Universitat Autònoma de BarcelonaBarcelonaSpain Pediatric Allergy and Pulmonology and Cystic Fibrosis UnitHospital Universitari Vall d’HebronBarcelonaSpain Consultorio Sevilla la Nueva MadridSpain Centro de Salud Dos de Mayo MadridSpain Hospital Miguel Servet ZaragozaSpain Hospital Universitario Araba Vitoria-GasteizSpain Departamento de Farmacologia Pediatría y RadiologíaUniversidad de SevillaSevilleSpain Institut d’Investigaciói InnovacióParc Taulí SabadellSpain

Asthma in school-aged children is a major public health problem worldwide[1,2].Inhaled medications are the mainstay of its pharmacological management[2],but only 8%-22%of children with asthma use their inhalers correc... 详细信息

关键词： asthma pediatric patients

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Erratum to “Dietary Quality and Comorbidities of Adults at Medical risk for Diabetes” [Open Journal of Preventive Medicine 4 (2014) 138-144]

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Open Journal of Preventive Medicine 2014年第6期4卷 513-513页

作者： joan A. Vaccaro Daniela Safina Lauren Grunspan Fatma G. Huffman Department of Dietetics and Nutrition Florida International University Miami USA

The original online version of this article (Vaccaro, J.A., Safina, D., Grunspan, L. and Huffman, F.G. (2014) Dietary Quality and Comorbidities of Adults at Medical risk for Diabetes. Open Journal of Preventive Medicine, 4, 138-144. http://***/10.4236/ojpm.2014.44019) unfortunately contains a mistake. The authors wish to correct the errors in Table 4, page 142: P-value for the variable Special diet (yes) should read 0.081. And they added (yes) after Special diet for clarity. They moved race/ethnicity up and presented it without an Or, for clarity.

关键词： Prediabetes Dietary Quality Hypercholesterolemia Obesity Hypertension

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