检索结果-南通市图书馆

Effect of Menopause on Gene Expression Profiles of Circu-lating Monocytes: A Pilot in vivo Microarray Study

Journal of Genetics and Genomics 2007年第11期34卷 974-983页

作者： Dvornyk Volodymyr 刘耀中陆燕沈汇 lappe joan M 雷署丰 Recker Robert R 邓红文湖南师范大学生命科学学院分子与统计遗传学研究室克瑞屯大学骨质疏松研究中心

Menopause is one of the key physiological events in the female life and can increase the risk for a number of complex autoimmune, neurodegenerative, metabolic, and cardiovascular disorders. Circulating monocytes can differentiate into various cell types and play an important role in tissue morphogenesis and immune response. We studied gene expression profiles of peripheral blood monocytes in healthy pre- and postmenopausal women using Affymetrix Human U133A GeneChip array that contains probes for -14,500 genes. Comparative analyses between the samples showed that 20 genes were up- and 20 were down-regulated. Of these genes, 28 were classified into six major GO categories relevant to such biological processes as the cell proliferation, immune response, cellular metabolism, and the others. The remaining 12 genes have yet unidentified biological functions. Our results support the hypothesis that functional state of circulating monocytes is indeed affected by menopause, and resulting changes may be determined through the genomewide gene expression profiling. Several differentially expressed genes identified in this study may be candidates for further studies of menopause-associated systemic autoimmune, neurodegenerative, and cardiovascular disorders. Our study is only the first attempt in this direction, but it lays a basis for further research.

关键词： menopause monocytes, microarrays differential gene expression

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

补钙结果可能不宜泛化

引用

英国医学杂志中文版 2008年第2期11卷 126-127页

作者： joan M lappe Robert P Heaney Creighton University 何小魁(译) 汪谋岳(校) 护理学和内科学教授 John A Creighton大学内科学教授 Omaha NE68178USA 不详

Bolland等研究表明，给健康的绝经后妇女补钙，血管事件的危险性有所增加，特别是心肌梗死事件。我们最近完成了一项类似研究，采用主要的骨骼终点。我们根据自己的资料对Bolland等报道的主要的不良事件提出质疑。

关键词：补钙绝经后妇女心肌梗死不良事件

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Comprehensive analysis of the association of EGFR,CALM3 and SMARCD1 gene polymorphisms with BMD in white women

Comprehensive analysis of the association of EGFR,CALM3 and ...

引用

第十二届全国青年药学工作者最新科研成果交流会

作者： Qiu-Hong Zhou Lan-Juan Zhao Ping Wang Rhamee Badr Xiao-Jing Xu Feng-Xiao Bu joan lappe Robert Recker Yu Zhou An Ye Bo-Ting Zhou Department of Endocrinology Xiangya HospitalCentral South University Osteoporosis Research Center Creighton University Medical Center Creighton University 601 N3Oth STSuite 4820OmahaNE 68131USA Department of Biostatistics & Bioinformatics School of Public Health and Tropical MedicineTulane UniversityNew OrleansLA 70112USA Department of Pharmacy Xiangya HospitalCentral South University Tulane University School of Medicine New OrleansLA 70112USA

Three genes EGFR（epidermal growth factor receptor）,CALM3（calmodulin 3,calcium-modulated protein 3,） and SMARCD1（SWI/SNF related,matrix associated,actin dependent regulator of charomatin,subfamily d,member 1） in white women played different roles in bone and/or fat *** the population-based investigation of 870 unrelated postmenopausal white women,CALM3 polymorphisms were significantly associated with femoral neck bone mineral density（FNK BMD）,hip BMD and spine *** and tobacco status also affected these BMD levels and hence were corrected for in our statistical ***:EGFR,CALM3 and SMARCD1 play their part in bone and/or fat metabolism,however,the correlation between polymorphisms of these three genes and body composition levels including BMD is yet *** and Methods:A population-based investigation of 870 white women was conducted.44 SNPs（Single Nucleotide Polymorphisms） in EGFR,CALM3 and SMARCD1 were chosen by the software of Haploview version 4.2,including those with potential functional *** candidate SNPs were genotyped by KASPar assay for association analysis with body composition levels at different *** correlation analysis was performed by the Pearson’s product-moment and Spearman rank-order test,and the family-wise error was corrected using the Wald test implement in ***:rsl2461917 in the 3’-flanking region of CALM3 gene was significantly associated wim FNK BMD（P=0.001）,hip BMD（PO.001） and spine BMD（P=0.001）;rsll083838 in the 5’-flanking region of CALM3 gene was associated with spine BMD（P=0.009）.After adjusting for multiple comparisons,rsl2461917 remained significant（Padjusted=0.033 for FNK BMD,Padjusted=0.006 for hip BMD and Padjusted=0.018 for spine BMD）.Conclusions:Our data shows that polymorphisms of the CALM3 gene in white women could contribute to variation in BMD at the hip,spine,and femoral neck.

关键词： osteoporosis bone mineral density genetics single nucleotide polymorphisms EGFR CALM3 SMARCDl

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

人骨髓间充质干细胞来源的脂肪细胞在成骨细胞发生过程中抑制作用机制的研究

人骨髓间充质干细胞来源的脂肪细胞在成骨细胞发生过程中抑制作用...

引用

中华医学会第三次骨质疏松和骨矿盐疾病中青年学术会议

作者：逯伟达 Hengwei Zhang Yingchun Zhao Rui Cao Peijing Rong Weikuan Gu Jing Xiao Dengshun Miao joan lappe Robert Recker 高海青 Gary Guishan Xiao 山东大学齐鲁医院老年病科 Genomics & Functional Proteomics Laboratory Osteoporosis Research CenterCreighton UniversityMedical CenterU.S.A 中国中医科学院 Department of Orthopaedic Surgery-Campbell Clinic University of TennesseeU.S.A 南京医科大学解剖教研室

目的:近年来研究发现骨质疏松患者骨髓中有大量脂肪细胞浸润且与骨量减少与骨丢失是成正比的。本实验旨在研究骨髓间充质干细胞来源的脂肪细胞在成骨细胞发生分化及骨形成过程中的抑制作用机制。方法:Ⅰ.细胞共培养使用三种不用浓度的... 详细信息

目的:近年来研究发现骨质疏松患者骨髓中有大量脂肪细胞浸润且与骨量减少与骨丢失是成正比的。本实验旨在研究骨髓间充质干细胞来源的脂肪细胞在成骨细胞发生分化及骨形成过程中的抑制作用机制。方法:Ⅰ.细胞共培养使用三种不用浓度的脂肪细胞诱导剂诱导骨髓间充质干细胞向脂肪细胞方向分化,14天后与骨髓间充质干细胞共培养,同时向成骨细胞分化方向诱导。共培养有两种模式:脂肪细胞和干细胞直接接触(ModeA)和非直接接触(Mode B)。Ⅱ.形态学分析细胞向成骨细胞分化方向诱导14天后进行碱性磷酸酶(ALP)染色,28天后进行钙化结节染色。Ⅲ.mRNA microarray分别收集Mode B 3组下层细胞提取RNA进行比较分析。ⅣTwo-dimensional Gel Electro-

关键词：脂肪细胞浸润成骨细胞发生过程骨质疏松抑制机制

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：