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Integrative Zoology 2024年第3期19卷 417-423页

作者： joão pedro rueda furlan Micaela Santana RAMoS Fábio Parra SELLERA Irys Hany Lima GoNZALEZ Patrícia Locosque RAMoS Eliana Guedes STEHLING Department of Clinical Analyses Toxicology and Food ScienceSchool of Pharmaceutical Sciences of Ribeirão PretoUniversity of São PauloRibeirão PretoBrazil Department of Internal Medicine School of Veterinary Medicine and Animal ScienceUniversity of São PauloSão PauloBrazil School of Veterinary Medicine Metropolitan University of SantosSantosBrazil Coordination of Wild Fauna Secretary of EnvironmentInfrastructure and LogisticsSão PauloBrazil

Antimicrobial resistance(AMR)is a growing phenomenon that poses a major public health challenge worldwide(Dhingra et al.2020;Antimicrobial Resistance Collaborators 2022).The selective pressure exerted in clinical settings has contributed to the longlasting successful dissemination of multidrug-resistant(MDR)Gram-negative bacteria in these environments(Chia et al.2020).

关键词： multidrug clinical

Genomic characterization of multidrug-resistant extraintestinal pathogenic Escherichia coli isolated from grain culture soils

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Pedosphere 2022年第3期32卷 495-502页

作者： joão pedro rueda furlan Inara Fernanda Lage GALLo Eliana Guedes STEHLING Departamento de Análises Clínicas Toxicológicas e BromatológicasFaculdade de Ciências Farmacêuticas de Ribeirão PretoUniversidade de São PauloRibeirão PretoSão Paulo 14040-903(Brazil)

Multidrug-resistant(MDR)Escherichia coli,mainly extraintestinal pathogenic ***(ExPEC),has been widely reported in infections *** agricultural soils,manure is a hotspot for the dissemination of antimicrobial resistance genes(ARGs)and pathogenic bacteria;however,MDR bacteria have also been reported in soils with no history of manure *** addition,cross-resistance and co-resistance have been described as responsible for the metal-driven selection of bacteria resistant to ***,the aim of this study was to analyze three MDR *** isolates obtained from Brazilian grain culture soil samples with no history of manure use by whole-genome *** MDR *** isolates were recovered from soils from corn and coffee fields,and presented resistance toβ-lactams,quinolones,aminoglycosides,tetracyclines,sulphonamides,and dihydrofolate reductase *** analysis showed ARGs to several antimicrobials(i.e.,β-lactams,tetracyclines,aminoglycosides,sulphonamides,trimethoprim,phenicols,fosfomycin,and macrolides)as well as several metal resistance genes and antibacterial biocide resistance *** addition,known mutations in quinolone-resistance-determining regions of GyrA(Ser83Leu and Asp87Asn),ParE(Ser458Thr),and ParC(Ser80Ile)were also *** analysis showed the presence of virulence genes(lpfA,mcmA,gad,mchF,iroN,cma,and iss)associated with ***-resistant ExPEC isolates were assigned to phylogenetic group *** presence of MDR B1-ExPEC in soil samples shows the ability of these isolates to survive in *** study reports for the first time some sequence types(i.e.,ST345,ST448,and ST1146)of MDR *** in Brazilian ***,these findings contribute to the monitoring of antimicrobial resistance and surveillance studies based on whole-genome sequencing worldwide.

关键词： antimicrobial resistance gene metal resistance gene molecular typing whole-genome sequencing

Molecular characterization of an extensively drug-resistant Acinetobacter baumannii isolated from a corn culture soil

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Pedosphere 2021年第6期31卷 973-976页

作者： joao pedro rueda furlan Eliana Guedes STEHLING Departamento de Analises Clinicas Toxicologicas e BromatologicasFaculdade de CienciasFarmaceuticas de Ribeirao PretoUniversidade de Sao Paulo(USP)Ribeirao Preto SP 14040-903(Brazil)

Dear Editor,Soil is the most biodiverse habitat on Earth and it has been estimated that one gram of soil can contain up to one billion bacterial cells(Fierer,2017).The microorganisms of soil produce a wide variety of ... 详细信息

关键词： soil corn culture

Presence of blaoXA-48-carrying IncF plasmid in an Escherichia fergusonii strain isolated from a sugarcane soil

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Pedosphere 2020年第2期30卷 293-294页

作者： Jessica Aparecida Silva MoRETTo joao pedro rueda furlan Vania Santos BRAZ Tamires Aparecida BITENCoURT Eliana Guedes STEHLING Departamento de Analises Clinicas Toxicologicas e BromatologicasFaculdade de Ciencias Farmaceuticas de Ribeirao PretoUniversidade de Sao PauloRibeirao Preto SP 14040-903(Brazil Departamento de Genetica Faculdade de Medicina de Ribeirao PretoUniversidade de Sao PauloRibeirao Preto SP 14040-903(Brazil

Dear Editor,A large number of antimicrobial agents used for the treatment of humans and animals reach water sources and agricultural soils in different ways,including animal ***,antimicrobial-resistant bacteria,carrying important antimicrobial resistance genes(ARGs),circulate between humans,animals,and the environment,giving attention to the concept of one Health(Topp,2017;Tiedje et al.,2019).

关键词： soil soils sugarcane

Determination of critical state line(CSL)for silty-sandy iron ore tailings subjected to low-high confining pressures

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journal of Rock Mechanics and Geotechnical Engineering 2024年第5期16卷 1684-1695页

作者： Nilo Cesar Consoli joão Vítor de Azambuja Carvalho Alexia Cindy Wagner Hugo Carlos Scheuermann Filho Inácio Carvalho pedro Pazzoto Cacciari joão Paulo de Sousa Silva Civil Engineering Universidade Federal do Rio Grande do SulPorto AlegreRS90035-190Brazil Exploration and Mineral Projects-Mineral Development Centre VALE S.A.Santa LuziaMG33040-900Brazil Department of Civil Geological and Mining EngineeringPolytechnique MontrealQuebecH3C 3A7Canada

The disposal of filtered tailings in high dry stacks can induce particle breakage,changing the material's behaviour during the structure's *** grading changes influence material properties at the critical state,and this is not mature for intermediate artificial soils(tailings)in a broad range of confining *** this paper,it aims to describe the behaviour of iron ore tailings in a spectrum of confining pressures broader than the reported in previous studies.A series of consolidated drained(CD)triaxial tests was carried out with confining pressures ranging from 0.075 MPa to 120 *** results show that the amount of breakage plays an essential role in the response of iron ore *** existence of curved critical state line(CSL)in both specific volume(ν)-logarithm of mean effective stress(p′)and deviatoric stress(q)-mean effective stress(p′)planes,and different responses in the deviatoric stress-axial strain-volumetric strain curves were *** inverse S-shaped equation was proposed to represent the silty-sandy tailings'behaviour up to high pressures onν-lnp′*** proposed equation provides a basis for enhancing constitutive models and considers the evolution of the grading up to severe loading *** adjustment considered three regions with different responses associated with particle breakage at different pressure levels.

关键词： Tailings Iron ore tailings dry stacking Silty-sandy material Critical state soil mechanics High confining pressures Particle breakage

Comparison of Surgical Techniques Used in Treating Acromioclavicular Dislocation in Patients Participating in Sports: A Systematic Review

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open journal of orthopedics 2024年第1期14卷 41-52页

作者： Walter Hugo Brandão Nascimento Paulo Renan Matos Sucupira Cunha joão pedro Pimentel Abreu Lethycia Pereira Rosa Kamilly Iêda Silva Veigas Rodrigo Martins Silva Caetano orthopedic and Trauma Surgeon Brazilian Army Rio de Janeiro Brazil orthopedic and Trauma Surgeon S&atildeo Luís Brazil Scholarship Holder National Council for Scientific and Technological Development (CNPq) S&atildeo Luís Brazil School of Medicine Federal University of Maranh&atildeo (UFMA) S&atildeo Luís Brazil orthopedic and Trauma Surgeon Shoulder and Elbow Surgery Specialist University Hospital of the Federal University of Maranh&atildeo (HUUFMA/EBSERH) S&atildeo Luís Brazil

Introduction: The acromioclavicular joint is a superficial diarthrodial joint that surrounds the medial articular facet of the acromion and the distal portion of the clavicle. Due to its anatomy and biomechanics, it is highly susceptible to trauma and in young men who play contact sports, acromioclavicular dislocation is common. This article aimed to systematically review the literature and compare the surgical techniques used in the treatment of acromioclavicular dislocation in patients who practice sports. Methods: This systematic review was conducted according to the International Preferred Reporting Items for Systematic Review and Meta-Analyses guidelines. Eligible studies for this systematic review included articles in English or Spanish published between 2013 and 2023, which mention the occurrence of acromioclavicular dislocation during sports practices. Additionally, only studies that addressed the surgical treatment of acromion-clavicular dislocation and contained original data on the topic were included. Results: We found 144 eligible studies after searching the LILACS and PubMed databases. Based on the inclusion and exclusion criteria and the reviewers’ consensus, we selected four studies for the systematic review. 133 patients with AC joint displacement were evaluated. Mean Age: approximately 31.90 years. 81.92 of these injuries occurred during sports practice. Surgical Procedures Used: titanium plates fixation (49 patients), arthroscopy (24), single tunnel technique (30) and coracoid sling technique (30). The results of the visual analog scale and Constant-Murley scores varied between the techniques used. Twenty-two complications after surgical treatment were identified. Conclusion: A significant variability of operative techniques can be used in the surgical approach of acromioclavicular dislocation, such as arthroscopy, single tunnel, coracoid sling and titanium plates. Although it presented excellent functional results compared to the other three tech

关键词： Acromioclavicular joint Shoulder Dislocation Surgical Procedure Postoperative Complications Postoperative Care

Antioxidants Enzymes Activities and No Levels in Hemodialysis and Continuous Ambulatory Peritoneal Dialysis—A Comparative Study

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journal of Biosciences and Medicines 2024年第2期12卷 50-59页

作者： Natalia Cristina Aparecida Ribeiro e Ribeiro Anderson Martins Tavares Jaslana Hainfellner da Silva joão Paulo Lima Daher Salim Kanaan Luciene de Carvalho Cardoso-Weide Laboratory of Neuroplasticity Neurobiology Department Biology Institute Federal Fluminense University Rio de Janeiro Brazil Postgraduate Program in Medical Sciences School of Medicine Federal Fluminense University Rio de Janeiro Brazil School of Biology UNIVERSO—Salgado de Oliveira University Rio de Janeiro Brazil Department of Pathology Faculty of Medicine Federal Fluminense University Rio de Janeiro Brazil

It consists of a retrospective study with twenty-two individuals diagnosed with DRC: fourteen HD and eight CAPD, followed up in the dialysis center of Antonio pedro University Hospital (HUAP) of Fluminense Federal University (UFF), between 2015 and 2017. Fifteen healthy control (HC) subjects were included, without diagnosed CKD. Patients with HIV positive, hepatitis A, B, and C, pregnant, cancer, smokers, alcoholics and those exposed to X-rays in the last 3 months, were excluded. objectives: As oxidative stress and endothelial dysfunction may be linked to the higher prevalence of CVD in CKD patients, we measured the activities of the antioxidants enzymes: SoD and GPx and total No levels in the plasma and serum of end-stage CKD patients undergoing dialysis therapy, comparing with the HC group. Methods: Quantification of No levels was performed by fluorometric kit, while activities of SoD and GPx were determined using kinetic methods. Results: We found higher plasma SoD activity in HD (8.58 U/ml) and CAPD (10.14 U/ml), compared to C (3.73 U/ml) group, while GPx activity was decreased in HD (115.38 nmol/h/ml) and CAPD (122.76 nmol/h/ml) groups compared to HC group (275.83 nmol/h/ml). Total serum No concentration was decreased in HD (14.09 pmol/μl) and CAPD (10.26 pmol/μl), compared to non-CKD patients (49.65 pmol/μl). Conclusion: Decreased total serum No and GPx activities may lead to endothelial dysfunction and consequently a higher prevalence of CVD in CKD patients.

关键词： Hemodialysis CAPD SoD GPx No

Bilateral hip heterotopic ossification with sciatic nerve compression on a paediatric patient–An individualized surgical approach: A case report

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World journal of orthopedics 2022年第8期13卷 768-774页

作者： joão pedro Gouveia Nóbrega pedro jordão joana Arcângelo Department of orthopaedics Hospital Dr.Nélio MendonçaFunchal 9000-243Portugal Department of orthopaedics Hospital Dona EstefâniaLisboa 1169-045Portugal

BACKGRoUND Neurogenic heterotopic ossification is an acquired serious complication described in patients with central nervous system disorders and defined by bone formation in non-osseous *** SUMMARY We present an unusual case of a 13-yr-old boy presenting with hip pain and severe gait impairment 5 mo after the diagnosis of hemiplegia following a spontaneous intracerebral *** tomography revealed bilateral heterotopic ossification of both the paretic and the non-paretic limbs,with entrapment of the sciatic *** choice of surgical or nonsurgical management of such patients depends on the timing of diagnosis,the symptoms,and the extent of maturation of the ossified *** resection remains the only treatment with proven,evidence-based *** choice of surgical approach largely depends on the location of the ossified *** We believe the plane of dissection presented is a satisfactory option for resection of a posteromedial mass and sciatic nerve release.

关键词： Hip Heterotopic ossification Pediatrics orthopedics Ressection surgery Case report

Best practices for electrochemical characterization of supercapacitors

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journal of Energy Chemistry 2023年第5期80卷 265-283,I0007页

作者： joão pedro Aguiar dos Santos Fernando Cesar Rufino joǎo IYutaka ota Rodolfo CFernandes Rafael Vicentini Cesar JBPagan Leonardo Morais Da Silva Hudson Zanin Brazilian Renewable Energies Center for Innovation on New EnergiesSchool of Electrical and Computer EngineeringUniversity of CampinasSP 13083-852Brazil School of Electrical and Computer Engineering University of CampinasSP 13083-852Brazil Department of Chemistry Laboratory of Fundamental and Applied ElectrochemistryFederal University of Jequitinhonha e Mucuri's ValleyRodovia MGT 367km 5835000Alto da Jacuba39100-000 DiamantinaMGBrazil

We discuss here essential aspects of the experimental supercapacitors characterization by a series of well-known electrochemical *** are motivated by a considerable number of publications that misreport procedures and *** often conceal or neglect essential information about the electrochemical analytical apparatus used and its *** lack of such information may lead researchers,especially inexperienced ones,to misunderstand the procedures and ***,the misled electrochemical equipment configuration favors misinterpretation of data and low reproducibility *** paper aims to highlight these issues and clarify *** explain fundamental concepts of some electrochemical analytical methods,such as cyclic voltammetry,galvanostatic charge-discharge,single potential step chronoamperometry,and electrochemical impedance spectroscopy,focusing on the supercapacitor *** configurations of electrical parameters are presented and discussed to highlight the effects of incorrect setup and uncover misleading *** discuss how the electrochemical setup and data analyses matter in reliable data results for the supercapacitor.

关键词： Supercapacitor Electrochemical instrumentation Electrochemical methods Electrochemistry

Trustworthy evidence- based versus untrustworthy guidelines: detecting the difference

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Family Medicine and Community Health 2023年第4期11卷 85-90页

作者： joão pedro Lima Wimonchat Tangamornsuksan Gordon H Guyatt Department of Health Research Methods Evidenceand ImpactMcMaster UniversityHamiltonOntarioCanada

Guidelines are essential tools in healthcare *** guidelines inform clinicians not only on the direction(against or in favour)and strength(strong or weak/conditional)of recommendations but also on the certainty of the underlying *** trustworthy guidelines requires panellists with clinical and methodological expertise who consider patients’values and *** to trustworthiness standards remains variable;clinicians should,therefore,be able to distinguish trustworthy from untrustworthy *** this paper,we offer eight domains of disparities between trustworthy evidence-based guidelines and less trustworthy guidelines.

关键词： worthy trust lists