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Antarctic Macroalgae palmaria decipiens (Rhodophyta, palmariales) Extracts present Antioxidant and Antitumor Activity against Colorectal Cancer

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Advances in Biological Chemistry 2022年第6期12卷 274-291页

作者： Marina Della Giustina Rafaele Frassini Sidnei Moura Fernando joel Scariot Sérgio Echeverrigaray Vanessa Sayuri osaki Franciane M. pellizari pio Colepicolo joão antonio pêgas henriques Janaina da Silva Crespo mariana roes.h-ely Laboratory of Applied Toxicology and Bioproducts University of Caxias do Sul Rio Grande do Sul RS Brazil Laboratory of Biosynthetic and Natural products University of Caxias do Sul Rio Grande do Sul RS Brazil Laboratory of Applied Microbiology Biotechnology Institute University of Caxias do Sul Rio Grande do Sul RS Brazil Laboratory of phytology and Quality of Marine Water Paraná State University Paranaguá Campus PR Brazil Laboratory of Biochemistry and Molecular Biology of Algae University of S&#227 o paulo S&#227 o paulo SP Brazil Exact Sciences and Engineering Area University of Caxias do Sul Rio Grande do Sul RS Brazil

Despite therapeutic advances in recent decades, colorectal cancer is still the third most frequent neoplasm worldwide, with significant morbidity and mortality in young and middle-aged adults. Therefore, advance in treatment options for patients who are afflicted with tumor subtypes without effective therapies is needed. Antarctica macroalgae are substances-producing organisms with important biological activities, in which antitumor properties are investigated, showing promising cytotoxic results. There are no reports so far showing antitumor activity of macroalgae palmaria decipiens extracts against colorectal tumors. This study aims to evaluate the effect of macroalgae p. decipiens extract from Antarctic on tumor cell hCT-116 and non-tumor cell haCaT lines. The phenolic compounds present were identified by high performance liquid chromatography. The antioxidant activity of the extracts was determined by the Dpph radical inhibition method and cytotoxicity was evaluated through MTT assay. Cell death events were identified using dual staining with acridine orange/ethidium bromide and flow cytometry. The quantification of phenolic compounds present in the extracts identified the presence of three main compounds among them is kaempferol. The metanolic extract showed inhibition within 72 h of treatment in hCT-116 and potential antioxidant activity. The results presented in this study point out an imbalance in the redox metabolism and also a loss of mitochondrial membrane potential integrity, most likely inducing cell death mechanisms after 72 h exposure to treatment with metanolic extract. These events could be observed by penetration of propidium iodide through membrane damage. The results indicate that the extract of the Antarctic macroalgae p. decipiens interferes in the mechanisms of action of colorectal cancer tumor cells, acting as a potential antitumor and antioxidant agent.

关键词： Antarctic Seaweeds Antitumor Activity Colorectal Cancer Antioxidant Activity

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Desmarestia anceps Montagne (phaeophyceae) against Colorectal Cancer Cells: Cytotoxic Activity and proapoptotic Effects

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Advances in Biological Chemistry 2022年第6期12卷 228-245页

作者： Rafaele Frassini Daniela Steffens Sidnei Moura Cesar Aguzzoli Aline paternostro Martins pio Colepicolo Mutue Toyota Fujii Nair S. Yokoya Cláudio Martin pereira De pereira Ana Cláudia phillipus Miriam De Barcellos Falkenberg joão antonio pêgas.henriques.mariana roes.h-ely Laboratory of Applied Toxicology and Bioproducts University of Caxias do Sul RS Brazil Laboratory of Biosynthetic and Natural products of Caxias do Sul RS Brazil Area of Exact Sciences and Engineering University of Caxias do Sul RS Brazil Laboratory of Biochemistry and Molecular Biology of Algae University of S&#227 o paulo S&#227 o paulo SP Brazil Nucleus Research phycology Institute of Botany S&#227 o paulo SP Brazil Laboratory of Lipidomics and Biorganic-LipidomicaBio Federal University of Pelotas Pelotas RS Brazil Study Group on Natural and Synthetic products (GEpRoNAS) Federal University of Santa Catarina SC Brazil

Impairment of apoptosis promotes abnormal cellular proliferation and accumulation of genetic alterations. Identifying bioactive compounds extracted from seaweeds that induce apoptosis in cancer cells may be explored as new agents for cancer chemoprevention and/or chemotherapy. The present study aimed to determine the chemical composition and biological activity of the chloroform crude extract and fractions from Antarctic seaweed Desmarestia anceps. The chloroform extract was obtained by three consecutive macerations and the fractionation by vacuum liquid chromatography. The chemical characterization of the extract and fractions was performed through gas Chromatography. The cytotoxicity of the crude extract and fractions was evaluated by the MTT assay. Cell death and cell cycle evaluation after 24 hours of exposure to chloroform extract were performed by flow cytometry. A total of 48 compounds were identified. The results indicate that chloroform extract and its fractions presented cytotoxic activity against hCT 116 cell line in a dose dependent-manner. proapoptotic events were observed after chloroform extract exposition, which promoted an increase of multinucleated cells and reduced cell viability. This study was the first to explore cytotoxic potential of seaweed D. anceps fractions against hCT colorectal cancer cell line, suggesting that these macroalgae may be a promising candidate against anticancer activity.

关键词： Antarctic Seaweeds Antitumor Activity Apoptosis proapoptotic Effect Colorectal Cancer

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