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RBP-Jk involved in neointema formation in Chronic Kidney Disease-Induced AVF Failure through regulating FSP-1 expression

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RBP-Jk involved in neointema formation in Chronic Kidney Dis...

Rosuvastatin‑Eluting Gold‑Nanoparticle‑Loaded Perivascular Wrap for Enhanced Arteriovenous Fistula Maturation in a Murine Model

细胞—生命的基础——中国细胞生物学学会2013年全国学术大会·武汉

作者： Yun Wang Anlin Liang William EMitch jizhong cheng Department of Cell Biology Third Military Medical University Division of Nephrology Baylor College of Medicine Department of Orthopaedics First Affiliated Hospital of Chongqing Medical University

The most favored vascular access for hemodialysis of patients with chronic kidney disease(CKD) is an arteriovenous fistula(AVF).Failure of an AVF occurs mainly because of vascular stenosis which reflects neointimal *** previous studies have found that Notch pathway involved in the neointimal formation in CKD induced AVF *** it has been reported Notch pathway is

关键词： Notch signaling neointema formation chronic kidney disease AVF FSP-1

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Advanced Fiber Materials 2023年第6期5卷 1986-2001页

作者： Carleigh Klusman Benjamin Martin Joy Vanessa D.Perez Allan John R.Barcena Marvin R.Bernardino Erin Marie D.San Valentin Jossana A.Damasco Huckie C.Del Mundo Karem A.Court Biana Godin Gino Martin Canlas Natalie Fowlkes Richard Bouchard jizhong cheng Steven Y.Huang Marites P.Melancon Department of Interventional Radiology The University of Texas MD Anderson Cancer CenterHouston 77030USA Baylor College of Medicine Houston 77030USA College of Medicine University of the Philippines Manila1000 ManilaPhilippines Department of Nanomedicine Houston Methodist Research InstituteHouston 77030USA Department of Chemistry Lamar UniversityBeaumont 77710USA Department of Veterinary Medicine and Surgery The University of Texas MD Anderson Cancer CenterHouston 77030USA Department of Imaging Physics The University of Texas MD Anderson Cancer CenterHouston 77030USA Section of Nephrology Department of MedicineSelzman Institute for Kidney HealthBaylor College of MedicineHouston 77030USA The University of Texas MD Anderson Cancer Center UTHealth Houston Graduate School of Biomedical Sciences Houston 77030USA

Arteriovenous fistulas(AVFs)are a vital form of AV access for patients requiring hemodialysis,but they link to overall morbidity and mortality when they fail to *** most common cause of AVF non-maturation is neointimal hyperplasia(NIH).To minimize the deleterious effects of NIH,a perivascular wrap composed of polycaprolactone(PCL),rosuvastatin(ROSU),and gold nanoparticles(AUNPs)was *** study assessed the impact of ROSU-eluting,radiopaque resorbable perivascular wraps on pathologic NIH in a chronic kidney disease(CKD)rodent model of *** PCL wraps containing AuNPs and/or ROSU were monitored for in vitro tensile strength,AuNP release,ROSU elution,and effect on cellular *** wraps were then implanted around an AVF in a CKD rodent model for in vivo ultrasound(US)and micro-computed tomography(mCT)*** specimens were collected for histological *** viability was preserved in the presence of both AuNP-and ROSU-containing *** vitro release of ROSU and AuNPs correlated with in vivo findings of decreasing radiopacity on mCT over ***-loaded wraps had higher radiopacity(1270.0-1412.0 HU at week 2)compared with other wraps(103.5-456.0 HU),which decreased over *** addition of ROSU decreased US and histologic measurements of *** reduced NIH seen with ROSU-loaded perivascular wraps suggests a synergistic effect between mechanical support and anti-hyperplasia ***,AuNP loading increased wrap ***,our results show that AuNP-and ROSU-loaded PCL wraps induce AVF maturation and suppress NIH while facilitating optimal implanted device visualization.

关键词： Arteriovenous fistula Chronic kidney disease Nanoparticles Polycaprolactone Radiopacity Rosuvastatin

Expression of Foreign Gene in Mycobacterium Regulated by Human Mycobacterium Tuberculosis Heat Shock Protein 70 Promoter

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Journal of Huazhong University of Science and Technology(Medical Sciences) 1997年第4期17卷 193-199页

作者：程继忠皇甫永穆冯作化梁驹卿肖红 Department of Medical Molecular Biology Research Center of Experimental Medicine Tongji Medical University Wuhan

The DNA fragments of 150bp length promoter 0f human Mycobacterium(M.) tuberculosis heat shock protein (hsp)7O and 650bp length foreign gene, the Schistosoma japonicum glutathione S-transferase(Sj26GST)gene,were obtained by amplification with polymerase chain reaction. And the 150p DNA sequence upstream initiation codon ATG of the human M. tuberculosis hsp7O promoter that contains the sequence TTGAG and ATCATA which consensus with E. coli promoter's -35 and-10 region respectively, as well as ribosome binding site GGAGG at position-12-8 upstream the ATG were determined by SangerDideoxyribonucleotide-mediated chain-termination method-Then, the human M. tuberculosis hsp70 promoter and Sj26GST cDNA were cloned into E. coli-mycobacteria shuttle plasmid pBCG-2000 to construct E. coli-Mycobacterium expression shuttle plasmid pBCG- Sj26 that can express Sj26GST *** M. smegmatis were electroporated and the positivecolonies were selected by kanamycin-The *** containing the vector pBCG-Sj26 can be induced by heating and hydrogen peroxide (H2O2) to express GST. The molecular weight of the recombinant GST(rGST) was 26000. The rGST contents that were about 10 percent of the total bacterial protein were analyzed by density scanning after running SDS-PAGE. This study would provide scientific evidences for application of hsp70 promoter in expressing foreign gene in mycobacterium and development of mycobacterium as multiple-valent vectoral vaccine.

关键词： mycobacterium heat shock protein promoter shuttle plasmid gene expression

人结核杆菌热休克蛋白70启动子对外源基因在分枝杆菌中表达的影响

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中华微生物学和免疫学杂志 1997年第6期17卷 410-415页

作者：程继忠皇甫永穆冯作化梁驹卿肖红同济医科大学实验医学研究中心医学分子生物学研究室

利用聚合酶链反应技术（Ｐｏｌｙｍｅｒａｓｅｃｈａｉｎｒｅａｃｔｉｏｎ，ＰＣＲ）扩增得到１５０ｂｐ的人结核杆菌热休克蛋白（Ｈｅａｔｓｈｏｃｋｐｒｏｔｅｉｎ，ＨＳＰ）７０启动子和约６５０ｂｐ的外源基因日本血吸虫谷胱甘肽硫... 详细信息

利用聚合酶链反应技术（Ｐｏｌｙｍｅｒａｓｅｃｈａｉｎｒｅａｃｔｉｏｎ，ＰＣＲ）扩增得到１５０ｂｐ的人结核杆菌热休克蛋白（Ｈｅａｔｓｈｏｃｋｐｒｏｔｅｉｎ，ＨＳＰ）７０启动子和约６５０ｂｐ的外源基因日本血吸虫谷胱甘肽硫转移酶（ＧｌｕｔａｔｈｉｏｎｅＳ－ｔｒａｎｓｆｅｒａｓｅ，ＧＳＴ）的ｃＤＮＡ片段。并采用Ｓａｎｇｅｒ双脱氧链终止法测定了人结核杆菌ＨＳＰ７０起始密码ＡＴＧ上游１５０ｂｐ的启动子序列。在这一区域可见与大肠杆菌（Ｅ．ｃｏｌｉ）－３５和－１０区同源的碱基顺序ＴＴＧＡＧ和ＡＴＣＡＴＡ，以及ＡＴＧ上游－１２～－８处的核糖体结合位点ＧＧＡＧＧ。然后将人结核杆菌ＨＳＰ７０启动子和日本血吸虫ＧＳＴ编码基因克隆到Ｅ．ｃｏｌｉ－分枝杆菌穿梭质粒ｐＢＣＧ－２０００中，构成能表达日本血吸虫ＧＳＴ基因的Ｅ．ｃｏｌｉ－分枝杆菌穿梭表达载体ｐＢＣＧ－Ｓｊ２６。电转化耻垢分枝杆菌后，用卡那霉素筛选出阳性重组子。含有ｐＢＣＧ－Ｓｊ２６的耻垢分枝杆菌在热和过氧化氢的作用下均诱导出ＧＳＴ的表达。其表达产物分子量为２６×１０３，经聚丙烯酰胺凝胶（ＳＤＳ－ＰＡＧＥ）后，扫描分析，表明重组ＧＳＴ（ｒＧＳＴ）占菌体总蛋白的１０％。本研究为Ｈ?

关键词：结核分枝杆菌热休克蛋白启动子基因表达

基于IPv6网与ZigBee无线传感网的协议转换网关研究

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科技传播 2010年第11期2卷 115-116,118页

作者：成继中彭真湖南人文科技学院通信与控制工程系湖南娄底417000 涟源钢铁集团有限公司质量检测中心湖南娄底417000

本文通过对无线传感网,ZigBee协议,IPV6技术的研究和分析,针对传感网处理速度慢,存储容量小等特点,对常用TCP/IPV6、ZigBee协议和协议栈进行了改变和简化,实现IPv6网与基于ZigBee协议的无线传感网的互联,课题的主要思路是如下3个方面:1... 详细信息

本文通过对无线传感网,ZigBee协议,IPV6技术的研究和分析,针对传感网处理速度慢,存储容量小等特点,对常用TCP/IPV6、ZigBee协议和协议栈进行了改变和简化,实现IPv6网与基于ZigBee协议的无线传感网的互联,课题的主要思路是如下3个方面:1)把无线传感网虚拟成一个IPv6网,实现传感节点与IPv6网逻辑上的互联;2)网关的协议转换模型设计,实现ZigBee分组与IPv6分组的转换和传送;3)面向安全思想的设计,解决无线传感网常规安全问题。测试结果表明所设计的转换协议栈适合于各种嵌入式设备,是一种解决ZigBee无线传感网接入IPV6网络的可行方案。

关键词： IPV6 ZigBee 无线传感网虚拟IPv6网

Mechanochemistry of catch bonds between integrins and ligands

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Mechanochemistry of catch bonds between integrins and ligand...

第九届全国生物力学学术会议

作者： Fang Kong~1,Wei Chen~1,jizhong Lou,cheng Zhu~(1.3*)(1,Woodruff School of Mechanical Engineering,2 Institute for Bioengineering and Bioscience,and 3,Coulter Department of Biomedical Engineering Georgia Institute of Technology, Atlanta,GA 30332-0363cheng)

Integrins are a large family of adhesion molecules broadly expressed on the surface of a wide variety of cells as *** of integrins to ligands provides anchorage and signals for the cell,making them prime candidates for mechanosensing *** elucidate how force regulates integrin/ligand dissociation,we used molecular mechanics experiments to measure lifetimes of single bonds of or L integrins interacting with their respective ligands,fi-

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外源基因在耻垢分枝杆菌中表达效率的研究

生物化学与生物物理进展 1997年第3期24卷 249-253页

作者：程继忠皇甫永穆海涛同济医科大学实验医学研究中心武汉430030 同济医科大学职业医学研究所

将外源基因———日本血吸虫 2 6K抗原 (Schistosomajaponicum 2 6Kantigen ,Sj2 6GST)基因克隆到大肠杆菌分枝杆菌穿梭质粒 pBCG 2 0 0 0中 ,构建四个不同的表达载体 ,研究了它们在耻垢分枝杆菌中的表达效率 .首先将含人结核杆菌热休... 详细信息

将外源基因———日本血吸虫 2 6K抗原 (Schistosomajaponicum 2 6Kantigen ,Sj2 6GST)基因克隆到大肠杆菌分枝杆菌穿梭质粒 pBCG 2 0 0 0中 ,构建四个不同的表达载体 ,研究了它们在耻垢分枝杆菌中的表达效率 .首先将含人结核杆菌热休克蛋白 70 (heatshockprotein ,hsp70 )启动子的质粒pMT 70用NcoⅠ切 ,进行两种不同的修饰 ,得到不同的SD序列 ,将Sj2 6GST基因克隆进去 ;再将含hsp70启动子和Sj2 6GST的基因片段克隆到 pBCG 2 0 0 0中 ,筛选出不同SD序列、不同方向和不同拷贝数的分枝杆菌表达载体四个 .所表达的天然重组Sj2 6GST在SDS PAGE上分子质量为 2 6ku处可见明显的表达蛋白带 .通过薄层扫描分析 ,发现表达质粒中 ,双拷贝启动子外源基因组合表达效率最高 ,是单拷贝组合的 1 6倍 .而不同的克隆方向和不同的SD序列 (两者相差 3个碱基 )对表达效率的影响不明显 .

关键词：分枝杆菌表达效率外源基因疫苗

北京广安门内燕蓟古城遗址出土数万枚战国刀币布币

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中国钱币 2009年第2期 30-32页

作者：程纪中梁学义

一、出土古币概况今北京西南地区之宣武区,广安门内大街是贯穿该地区的主干道。广义街5号院地处广安门内大街西端北侧,原是北京义利食品公司厂址,2006年7月,该地改建广益大厦的施工挖槽中,出土窖藏两陶罐,内藏战国中晚期燕、赵、魏、韩... 详细信息

一、出土古币概况今北京西南地区之宣武区,广安门内大街是贯穿该地区的主干道。广义街5号院地处广安门内大街西端北侧,原是北京义利食品公司厂址,2006年7月,该地改建广益大厦的施工挖槽中,出土窖藏两陶罐,内藏战国中晚期燕、赵、魏、韩、周等国古钱币约三万枚,其中一罐藏方足、（共4页)

关键词：古城遗址广安北京出土布币刀币战国西南地区

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行星保护标准体系研究

同济大学学报（自然科学版） 2024年第8期52卷 1199-1208页

作者：刘继忠陈杨王丰李洋徐航程旋徐孟娇深空探测实验室安徽合肥230000 国家航天局探月与航天工程中心北京100190 同济大学电子与信息工程学院上海201804

我国行星探测工程火星取样返回任务属于国际行星保护政策中的V类限制性返回任务。需开展严格的行星保护工作,避免地球和火星之间出现交叉生物污染,并进而推动取得重大科学发现。依据天问三号任务行星保护设计规范,结合工程任务特点,利... 详细信息

我国行星探测工程火星取样返回任务属于国际行星保护政策中的V类限制性返回任务。需开展严格的行星保护工作,避免地球和火星之间出现交叉生物污染,并进而推动取得重大科学发现。依据天问三号任务行星保护设计规范,结合工程任务特点,利用系统工程方法论,进行行星保护工作标准化研究和需求分析,开展行星保护标准体系顶层设计,从纵向和横向两个维度构建了天问三号任务行星保护标准体系框架,制定了天问三号任务行星保护标准规范明细表,支撑了天问三号任务行星保护标准规范体系化建设。该研究成果和经验对提升我国深空探测行星保护工作的标准化水平具有重要借鉴和实践意义。

关键词：标准化标准体系行星保护火星取样返回任务深空探测