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Effect of myosin heavy chain composition of muscles on meat quality in Laiwu pigs and Duroc

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Science China(Life Sciences) 2008年第2期51卷 127-132页

作者： HU HongMei, WANG jiying, zhu RongSheng, GUO JianFeng & WU Ying Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, China 1. Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences Jinan 250100 China

In order to explain the mechanism of high meat quality in Laiwu pigs and investigate the relation between myosin heavy chains (MyHC) composition and meat quality, meat quality analysis was conducted and mRNA expression of MyHC I, IIa, IIx, IIb was quantified by real-time fluorescence PCR in longissimus muscle (LM) and semimembranous muscle of Laiwu pigs and Duroc. The result indicated that, compared with Duroc, mRNA expression of MyHC IIa, IIx in LM and semimembranous muscle of Laiwu pigs was significantly increased, mRNA expression of MyHC IIb was dramatically decreased. However, the expression of MyHC I was not significantly affected by breeds. The correlation between mRNA expression of MyHC I, IIa, IIx in LM and meat color, pH value, marbling, intramuscular fat content was positive, but shear value of LM was negative. The relation between MyHC IIb mRNA expression and marbling, intramuscular fat content was dramatically negative, whereas shear value was strikingly positive, as well as fiber diameter, but without reaching statistical significance. Therefore, the composition of MyHC I, IIa, IIx, IIb affected meat quality, furthermore, expression of MyHC I, IIa, IIx, IIb mRNA prominently influenced meat characteristics, especially edible quality of muscle, suggesting that mRNA expression level of MyHC I, IIa, IIx, IIb can exactly and impersonally estimate meat quality.

关键词： Laiwu pigs myosin heavy chain real-time PCR meat quality

A novel in vitro prognostic model of bladder cancer based on urine-derived living tumor cells

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Genes & Diseases 2023年第6期10卷 2586-2596页

作者： Jiaqi Wang jiying zhu Junchi Hu Ziruoyu Wang Xiaobo Wang Jianbo Pan Yiwei Chu Zengxia Li Wei Jiang Chunmin Liang Jun Hou Jianming Guo Yongjun Dang Shuai Jiang Key Laboratory of Metabolism and Molecular Medicine Ministry of Education and Department of Biochemistry and Molecular BiologySchool of Basic Medical SciencesFudan UniversityShanghai 200032China Department of Immunology School of Basic Medical SciencesFudan UniversityShanghai 200032China Laboratory of Tumor Immunology Department of AnatomyHistologyand EmbryologySchool of Basic Medical SciencesFudan UniversityShanghai 200032China Center for Novel Target and Therapeutic Intervention Chongqing Medical UniversityChongqing 400016China Department of Pathology Zhongshan HospitalFudan UniversityShanghai 200032China Department of Urology Zhongshan HospitalFudan UniversityShanghai 200032China Department of Urology Zhongshan Hospital Wusong BranchFudan UniversityShanghai 200940China

Bladder cancer(BLCA)remains a difficult malignancy to manage because of its high recurrence,intense follow-up,and invasive diagnostic and treatment *** checkpoint inhibitors(ICIs)have forged a new direction for the treatment of BLCA,but it is currently challenging to predict whether an individual patient will be sensitive to *** collected 43 urine/tumor samples from BLCA patients for primary bladder cancer cells(BCCs)culturing using our previously reported BCC culture *** used flow cytometry(FCM)to measure the expression levels of Programmed Death-Ligand 1(PD-L1)on BCCs before and after interferon-gamma(IFN-γ)treatment and found that PD-L1 expression and the sensitivities to IFN-γvaried among ***-sequencing,western blotting,and programmed death-1(PD-1)binding assays confirmed that the BCC FCM-based PD-L1 detection platform(BC-PD-L1)was reliable and was not hindered by the glycosylation of *** the subsequent retrospective study,we found that IFN-γ-stimulated PD-L1(sPD-L1)expression on BCCs detected by BC-PD-L1 could predict the prognosis of BLCA ***,the prognostic value was similar or even better in urine-derived BC-PD-L1(UBC-PD-L1).Transcriptome analysis showed that BCCs with high sPD-L1 tended to enrich genes associated with the collagen-containing extracellular matrix,cell–cell adhesion,and positive regulation of the immune *** addition,the UBC-PD-L1 also exhibited predictive value for ICI response in BLCA *** conclusion,as a novel personalized urine-detection method,UBC-PD-L1 may provide a rapid,accurate,and non-invasive tool for monitoring tumor progression,predicting therapeutic responses,and helping improve BLCA clinical treatment in future.

关键词： Bladder cancer Immune checkpoint inhibitors Predictive model Primary cells Urine

Integrative multi-omics and drug-response characterization of patient-derived prostate cancer primary cells

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Signal Transduction and Targeted Therapy 2023年第6期8卷 3013-3028页

作者： Ziruoyu Wang Yanan Li Wensi Zhao Shuai Jiang Yuqi Huang Jun Hou Xuelu Zhang Zhaoyu Zhai Chen Yang Jiaqi Wang jiying zhu Jianbo Pan Wei Jiang Zengxia Li Mingliang Ye Minjia Tan Haowen Jiang Yongjun Dang Key Laboratory of Metabolism and Molecular Medicine The Ministry of EducationDepartment of Biochemistry and Molecular BiologySchool of Basic Medical SciencesShanghai Medical CollegeFudan University200032ShanghaiChina CAS Key Lab of Separation Sciences for Analytical Chemistry National Chromatographic Research and Analysis CenterDalian Institute of Chemical PhysicsChinese Academy of Sciences116023DalianChina The Chemical Proteomics Center and State Key Laboratory of Drug Research Shanghai Institute of Materia MedicaChinese Academy of Sciences201203ShanghaiChina University of Chinese Academy of Sciences 100049BeijingChina Department of Urology Zhongshan HospitalFudan University200032ShanghaiChina Department of Urology Zhongshan Hospital Wusong BranchFudan University200032ShanghaiChina Center for Novel Target and Therapeutic Intervention Chongqing Medical University400016ChongqingChina Department of Urology Huashan HospitalFudan University200040ShanghaiChina

Prostate cancer(PCa)is the second most prevalent malignancy in males across the world.A greater knowledge of the relationship between protein abundance and drug responses would benefit precision treatment for ***,we establish 35 Chinese PCa primary cell models to capture specific characteristics among PCa patients,including gene mutations,mRNA/protein/surface protein distributions,and pharmaceutical *** multi-omics analyses identify Anterior Gradient 2(AGR2)as a pre-operative prognostic biomarker in *** the drug library screening,we describe crizotinib as a selective compound for malignant PCa primary *** further perform the pharmacoproteome analysis and identify 14,372 significant protein-drug ***,the diminished AGR2 enhances the inhibition activity of crizotinib via ALK/c-MET-AKT axis activation which is validated by PC3 and xenograft *** integrated multi-omics approach yields a comprehensive understanding of PCa biomarkers and pharmacological responses,allowing for more precise diagnosis and therapies.

关键词： drug PC3 diagnosis operative

CT Perfusion Imaging Predicts One-Month Outcome in Patients with Acute Spontaneous Hypertensive Intracerebral Hemorrhage

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Advances in Computed Tomography 2013年第3期2卷 107-111页

作者： Huazhi Xu Weijian Chen Meihao Wang Guoquan Cao Yuxia Duan jiying zhu Department of Radiology The Affiliated Hospital of Wenzhou Medical College Wenzhou China.

Purpose: Little is known about the relationship between perihematomal perfusion parameters in acute spontaneous hypertensive intracerebral hemorrhage patients and recent outcome. The purpose of this study was to evaluate the relationship between the perfusion parameters of the perihematomal brain tissue and the recent prognosis of patients with acute spontaneous hypertensive intracerebral hemorrhage (shICH) using CT perfusion (CTP) imaging. Methods: Twenty-six patients with clinical and CT diagnosed supratentorial shICH received CTP scanning within 8 - 19 h after symptom onset. At the maximum levels of the hematoma, cerebral blood flow (CBF), cerebral blood volume (CBV) and mean transit time (MTT) of perihematomal area (isodense within 1cm rim of perilesion area on plain CT) and contralateral mirrored hemisphere were measured, and rCBF, rCBV, rMTT were calculated (ipsilateral/contralateral). The one-month follow-up in accordance with daily living table (Barthel index, BI) by telephone was recorded. Results: The CBV, CBF, and MTT values of perihematoma area were (1.61 ± 1.53) ml·100 g-1, (16.48 ± 12.58) ml·100 g-1·min-1, and (9.12 ± 2.57) s, respectively. (For more information,please refer to the PDF)

关键词： Cerebral Hemorrhage X-Ray Computed Tomography Perfusion Imaging Outcome

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双孢蘑菇采后贮藏品质预测模型

农业机械学报 2010年第6期41卷 103-107,112页

作者：王相友朱继英李霞山东理工大学农业工程与食品科学学院淄博255049

通过双孢蘑菇贮藏试验,研究不同温度和相对湿度对双孢蘑菇采后贮藏品质的影响,建立了动力学模型。当相对湿度为95%时,不同贮藏温度下双孢蘑菇失重率和硬度衰减率的回归模型分别为M=(1.394 9+0.351 9T)t,F=(2.415 3-0.020 4T+0.022 5T2)... 详细信息

通过双孢蘑菇贮藏试验,研究不同温度和相对湿度对双孢蘑菇采后贮藏品质的影响,建立了动力学模型。当相对湿度为95%时,不同贮藏温度下双孢蘑菇失重率和硬度衰减率的回归模型分别为M=(1.394 9+0.351 9T)t,F=(2.415 3-0.020 4T+0.022 5T2)t。贮藏温度为2℃时,不同相对湿度环境下双孢蘑菇失重率和硬度衰减率回归模型分别为Mr=(61.060 5-124.810 5RH+63.985R2H)t,Fr=(9.657 9-11.753 0RH+4.265 1R2H)t。结果表明,所得模型基本能预测双孢蘑菇在贮藏过程中的失重率和硬度衰减率的变化。经试验验证,模型预测值与试验值吻合良好。

关键词：双孢蘑菇贮藏品质预测模型

Synergistic effect of vermiculite and submerged plants on lake sediments

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Water Biology and Security 2023年第3期2卷 57-65页

作者： Rou Wang Yunli Liu Feng Luo Guoliang Bai Yadong Tang Qingjun Fang jiying zhu Beining Li Zisen Liu Feng He Qiaohong Zhou Zhenbin Wu Yi Zhang State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyChinese Academy of SciencesWuhan430072China University of Chinese Academy of Sciences Beijing100049China School of Environmental Studies China University of GeosciencesWuhan430074China School of Resources and Environmental Engineering Wuhan University of TechnologyWuhan430070China

The synergistic effect of vermiculite and the submerged macrophytes Vallisneria spiralis and Hydrilla verticillata on lake sediment was studied using diffusive gradients in thin films(DGT)*** dynamics of phosphorus(P)fractions in sediment,the labile-P and labile-S in the water-sediment continuum,and the microbial community in the rhizosphere were *** effectively promoted reproduction of microorganisms in the sediments Microbial abundance in treatments containing *** with sediments containing 10%added vermiculite,and *** containing 50%added vermiculite being 1.7 and 3.5 times higher than the controls which contained no added *** and Proteobacteria populations,which are both beneficial for the sediment microenvironment,were higher in treatment groups containing *** bioavailable-P in treatment groups containing added vermiculite was lower at the sediment-water interface,with a correlating decrease of TP by between 63%and 91%in the overlying *** suggests that vermiculite can affect the release of labile P and facilitate the assimilation of nutrients by macrophyte ***,vermiculite can improve the Oxidation-Reduction potential and further reduce sulfide toxicity to *** results provide theoretical guidance and technical support for the application of vermiculite combined with submerged plants for the remediation of eutrophic lakes.

关键词： Diffusive gradients in thin films(DGT) technique Physical and chemical properties Vermiculite Sediment microenvironment Submerged plant growth

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接种物耐酸驯化对菌糠厌氧干发酵产气的影响

农业工程学报 2020年第5期36卷 249-254页

作者：朱继英钟慧陆玉张岐山东理工大学农业工程与食品科学学院淄博255000

为了提高厌氧干发酵体系运行稳定性,使中间产物酸及时转化为甲烷,避免出现酸抑制现象,该研究采用逐步提高乙酸浓度降低pH值的方式对接种物进行驯化,得到了在乙酸浓度10200mg/kg、pH值6.0条件下仍能快速产气的耐酸接种物,并以易水解酸化... 详细信息

为了提高厌氧干发酵体系运行稳定性,使中间产物酸及时转化为甲烷,避免出现酸抑制现象,该研究采用逐步提高乙酸浓度降低pH值的方式对接种物进行驯化,得到了在乙酸浓度10200mg/kg、pH值6.0条件下仍能快速产气的耐酸接种物,并以易水解酸化的菌糠为原料进行了厌氧干发酵试验。结果表明,驯化过程中产甲烷古菌类群的多样性下降,乙酸营养型甲烷八叠球菌丰度大幅提高,乙酸转化率和甲烷浓度逐渐提高;耐酸接种物的脱氢酶活性下降,辅酶F420和纤维素酶活性升高;添加耐酸接种物可以加快菌糠厌氧干发酵启动速度,避免酸抑制现象的发生;接种物含有75%耐酸接种物的试验组甲烷产量提高了56.1%。该研究成果能够为有效解决厌氧干发酵过程酸抑制现象提供一定的理论指导。

关键词：甲烷发酵生物质

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贮藏温度对双孢蘑菇采后生理和品质的影响

农业机械学报 2005年第11期36卷 92-94,97页

作者：朱继英王相友许英超山东理工大学轻工与农业工程学院

研究了不同贮藏温度及贮藏过程中温度波动对双孢蘑菇采后生理和品质的影响。结果表明,低温可以抑制双孢蘑菇采后开伞、呼吸强度、PPO活性和褐变;而贮藏过程中温度波动将导致双孢蘑菇采后开伞率、呼吸强度提高,PPO活性和褐变度增加,对其... 详细信息

研究了不同贮藏温度及贮藏过程中温度波动对双孢蘑菇采后生理和品质的影响。结果表明,低温可以抑制双孢蘑菇采后开伞、呼吸强度、PPO活性和褐变;而贮藏过程中温度波动将导致双孢蘑菇采后开伞率、呼吸强度提高,PPO活性和褐变度增加,对其采后生理和品质造成严重影响。

关键词：双孢蘑菇贮藏温度波动采后生理

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气调包装下果蔬呼吸速率研究进展

农业机械学报 2008年第8期39卷 94-100,78页

作者：王相友李霞王娟朱继英山东理工大学轻工与农业工程学院

在气调包装(MAP)系统中,果蔬的呼吸作用和气体通过包装薄膜进行交换维持果蔬的微环境气体组成,而使果蔬的货架期延长。在果蔬的MAP系统设计中,果蔬的呼吸速率模型是成功的关键。本文综述了近几十年来国内外果蔬MAP中呼吸速率的研究进展... 详细信息

在气调包装(MAP)系统中,果蔬的呼吸作用和气体通过包装薄膜进行交换维持果蔬的微环境气体组成,而使果蔬的货架期延长。在果蔬的MAP系统设计中,果蔬的呼吸速率模型是成功的关键。本文综述了近几十年来国内外果蔬MAP中呼吸速率的研究进展,包括果蔬MAP中呼吸速率的测定方法,影响呼吸速率的因素和果蔬呼吸速率模型的研究动态。并在此基础上提出了目前果蔬MAP呼吸速率研究中存在的问题和进一步研究方向。

关键词：贮藏果蔬气调包装呼吸速率模型