检索结果-南通市图书馆

Pan-cancer analysis identifies RNA helicase DDX1 as a prognostic marker

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Phenomics 2022年第1期2卷 33-49页

作者： Baocai Gao Xiangnan li Shujie li Sen Wang Jiaxue Wu jixi li State Key Laboratory of Genetic Engineering School of Life SciencesMOE Engineering Research Center of Gene TechnologyShanghai Engineering Research Center of Industrial MicroorganismsFudan UniversityShanghai 200438China Kunming Institute of Physics Kunming 650223China

The DEAD-box RNA helicase(DDX)family plays a critical role in the growth and development of multiple ***1 is involved in mRNA/rRNA processing and mature,virus replication and transcription,hormone metabolism,tumo-rigenesis,and tumor ***,how DDX1 functions in various cancers remains ***,we explored the potential oncogenic roles of DDX1 across 33 tumors with The Cancer Genome Atlas(TCGA)and the Genotype-Tissue Expression(GTEx)***1 is highly expressed in breast cancer(BRCA),cholangiocarcinoma(CHOL),and colon adenocarcinoma(COAD),but it is lowly expressed in renal cancers,including kidney renal clear cell carcinoma(KIRC),kidney chromophobe(KICH),and kidney renal papillary cell carcinoma(KIRP).Low expression of DDX1 in KIRC is cor-related with a good prognosis of overall survival(OS)and disease-free survival(DFS).Highly expressed DDX1 is linked to a poor prognosis of OS for adrenocortical carcinoma(ACC),bladder urothelial carcinoma(BLCA),KICH,and liver hepatocellular carcinoma(liHC).Also,the residue Ser481 of DDX1 had an enhanced phosphorylation level in BRCA and ovarian cancer(OV)but decreased in *** infiltration analysis exhibited that DDX1 expression affected CD8+T cells,and it was significantly associated with MSI(microsatellite instability),TMB(tumor mutational burden),and ICT(immune checkpoint blockade therapy)in *** addition,the depletion of DDX1 dramatically affected the cell viability of human tumor-derived cell ***1 could affect the DNA repair pathway and the RNA transport/DNA replication processes during tumorigenesis by analyzing the CancerSEA ***,our pan-cancer analysis revealed that DDX1 had complicated impacts on different cancers and might act as a prognostic marker for cancers such as renal cancer.

关键词： RNA helicase DDX1 Pan-cancer analysis Survival analysis Prognostic marker

Phase separation of Gasdermin D regulates pyroptotic cell death

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Phase separation of Gasdermin D regulates pyroptotic cell de...

Crystal structure of a GH39 β-xylosidase Cm Xyn B1 from a deep-sea bacterium Croceicoccus marinus E4A9T

2021年全国生物相分离和相变学术研讨会

作者： Wenqing Gao Yuanyuan li jixi li State Key Laboratory of Genetic Engineering School of Life SciencesFudan University

Gasdermin-D（GSDMD） translocates to plasma membrane and forms pore-like structures upon stimulation by activated inflammasomes and bacterial lipid polysaccharide（LPS）.We have shown that TRIM promotes GSDMD to form high-order oligomers and positively regulates cell *** formed liquid-like phase separation upon stimulation by *** promoted GSDMD phase separation to regulate cell *** separation of GSDMD was mediated by the N-terminal domain and regulated by the C-terminal *** metal ions enhanced cell pyroptosis by promoting GSDMD phase *** results demonstrated that TRIM induced phase separation of GSDMD regulates pyroptotic cell death.

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Crystal structure of a GH39 β-xylosidase Cm Xyn B1 from a d...

Enzymatic characterization and crystal structure of a novel carboxylesterase E8

中国生物化学与分子生物学会2016年全国学术会议

作者： Yanfang Shen Jing Huang Ying-Yi Huo Jun Zheng Xue-Wei Xu jixi li State Key Laboratory of Genetic Engineering Collaborative Innovation Center of Genetics and Development School of Life Sciences Fudan University Key Laboratory of Marine Ecosystem and Biogeochemistry Second Institute of OceanographyState Oceanic Administration

β-xylosidases are glycosidase hydrolases that hydrolyze short xylooligosaccharides into xylose units,which contribute to the final breakdown of plant cell-wall ***,we determined the three-dimensional structure of a GH39 β-xylosidases CmX ynB 1 from a marine bacterium Croceicoccus marinus,which is isolated from the East Pacific polymetallic

关键词： β-xylosidases 3-D structure mechanism

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Enzymatic characterization and crystal structure of a novel ...

大肠杆菌GhoS的纯化及X射线衍射分析（英文）

中国生物化学与分子生物学会2016年全国学术会议

作者： Jun Zheng Ying-Yi Huo Yanfang Shen Jing Huang Chaoneng Ji Xue-Wei Xu jixi li State Key Laboratory of Genetic Engineering Collaborative Innovation Center of Genetics and Development School of Life Sciences Fudan University Key Laboratory of Marine Ecosystem and Biogeochemistry Second Institute of OceanographyState Oceanic Administration

A carboxylesterase(E8)has been identified in a marine bacterium,Croceicoccus marinus from the East Pacific and has been cloned and overexpressed in Escherichia coli.E8 is a novel esterase that doesn’t belong to any known *** characterization revealed that E8 has a preference for p-nitrophenyl esters with shortchain acyl esters(shorter than 10 carbon

关键词： carboxylesterase crystal structure catalytic mechanism

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

复旦学报（自然科学版） 2018年第1期57卷 30-35页

作者：程云珊李继喜甘建华复旦大学生命科学学院生理学与生物物理学系上海200438

Toxin-antitoxin体系在细菌中发挥着许多关键性的作用.作为唯一的typeⅤTA系统的代表,GhoS能够剪切GhoT蛋白的mRNA,进而抑制GhoT的毒性.GhoS在溶液中有单体和二体两种不同的存在形式,而且两种形式均具有催化活性.尽管目前有一个GhoS的NM... 详细信息

Toxin-antitoxin体系在细菌中发挥着许多关键性的作用.作为唯一的typeⅤTA系统的代表,GhoS能够剪切GhoT蛋白的mRNA,进而抑制GhoT的毒性.GhoS在溶液中有单体和二体两种不同的存在形式,而且两种形式均具有催化活性.尽管目前有一个GhoS的NMR溶液结构已经报道,GhoS是如何二聚以及如何识别和降解底物的机制还不明确.本文中我们报道了GhoS F14A突变体的纯化和初步晶体学研究.GhoS晶体的衍射分辨率为1.5.晶体属于P21空间群,晶胞参数为a=51.0,b=37.3,c=52.3,α=90.0°,β=119.6°,γ=90.0°.晶体的溶剂含量为35%,每个不对称单元中含有两个蛋白分子.

关键词：毒素/抗毒素 GhoS mRNA切割干扰酶核酸内切酶

High-precision alkali-atom density measurement and control methods using light absorption for dual-beam SERF magnetometers

在线全文

学校读者我要写书评

暂无评论

Chinese Optics Letters 2024年第5期22卷 41-46页

作者：刘子傲陆吉玺胡朝晖李晓昱闫一凡詹迪李建利 Key Laboratory of Ultra-Weak Magnetic Field Measurement Technology Ministry of EducationSchool of Instrumentation and Optoelectronic EngineeringBeihang UniversityBeijing 100191China Zhejiang Provincial Key Laboratory of Ultra-Weak Magnetic-Field Space and Applied Technology Hangzhou Innovation InstituteBeihang UniversityHangzhou 310051China Hefei National Laboratory Hefei 230088China Shen Yuan Honors College Beihang UniversityBeijing 100191China

The alkali-atom density measurement method based on light absorption is highly suitable for a spin-exchange relaxationfree(SERF)atomic magnetometer because of its high-precision measurement and complete nonmagnetic *** this study,the optical rotation angle detection system based on polarization balance detection is utilized to realize the alkali-atom density real-time measurement without affecting magnetic field *** discovered that there exists an optimal frequency detuning of the probe light,which offers the highest sensitivity in alkali-atom density measurement and the lowest susceptibility to temperature fluctuations in terms of the scale *** contrast to conventional light absorption measurements based on pump light,this method demonstrated a threefold improvement in alkali-atom density measurement sensitivity while remaining immune to ambient magnetic fields and incident light intensity ***,we utilized this method to achieve closed-loop temperature control with an accuracy of 0.04℃.

关键词： light absorption temperature measurement and control dual-beam SERF magnetometer

Thermoplasma volcanium来源β-葡萄糖苷酶的酶学性质分析

在线全文

学校读者我要写书评

暂无评论

复旦学报（自然科学版） 2021年第5期60卷 636-645页

作者：刘阳王丹纪锐顾少华李继喜季朝能复旦大学生命科学学院上海200438 复旦大学遗传工程国家重点实验室上海200438

β-葡萄糖苷酶(BGL)是催化纤维素产生葡萄糖过程中的关键酶,在生物能源利用和食品领域发挥重要作用.本研究克隆了来源于Thermoplasma volcanium的β-葡萄糖苷酶(BGL)基因,并在大肠杆菌中进行表达,经纯化获得了Tv-BGL.酶学性质分析发现,T... 详细信息

β-葡萄糖苷酶(BGL)是催化纤维素产生葡萄糖过程中的关键酶,在生物能源利用和食品领域发挥重要作用.本研究克隆了来源于Thermoplasma volcanium的β-葡萄糖苷酶(BGL)基因,并在大肠杆菌中进行表达,经纯化获得了Tv-BGL.酶学性质分析发现,Tv-BGL在pH6.0和80℃时发挥最佳催化活力,以对硝基苯基-β-D-吡喃葡萄糖苷(pNPGlu)为底物时的K_(m)和V_(max)分别为0.7254 mmol/L和614.60 U/mg,K_(cat)/K_(m)=7.79×10^(5) mol^(-1)·L·s^(-1),以纤维二糖(Cellobiose)为底物时的K_(m)和V_(max)分别为55.63 mmol/L和636.06 U/mg,Kcat/Km=1.06×10^(4) mol^(-1)·L·s^(-1).另外,该酶具有较好的热稳定性,有机溶剂、离子以及葡萄糖耐受性.

关键词： β-葡萄糖苷酶糖苷水解酶热稳定葡萄糖耐受工业应用

非小细胞肺癌免疫治疗抗药物抗体对临床疗效及不良反应的影响

在线全文

学校读者我要写书评

暂无评论

第三军医大学学报 2021年第17期43卷 1673-1678页

作者：赵先兰李吉茜杨峤李世勋余永新李奉李梦侠孙建国陆军军医大学(第三军医大学)第二附属医院肿瘤科重庆400037 解放军第941医院超声诊断科西宁810007 陆军特色医学中心肿瘤科重庆400042

目的探索非小细胞肺癌(non-small cell lung cancer,NSCLC)患者接受免疫检查点抑制剂(immune checkpoint inhibitors,ICIs)治疗后产生的抗药物抗体(anti-drug antibody,ADA)对患者临床疗效及不良反应的影响。方法从陆军军医大学第二附... 详细信息

目的探索非小细胞肺癌(non-small cell lung cancer,NSCLC)患者接受免疫检查点抑制剂(immune checkpoint inhibitors,ICIs)治疗后产生的抗药物抗体(anti-drug antibody,ADA)对患者临床疗效及不良反应的影响。方法从陆军军医大学第二附属医院及陆军特色医疗中心收集2016年4月至2019年9月使用帕博利珠单抗或纳武利尤单抗的Ⅲ/Ⅳ期NSCLC患者共26例,在首次输注后2个月左右采集血样,使用酶联免疫法检测血浆中ADA水平,并分析ADA对ICIs的临床疗效、疾病进展及免疫相关不良反应(immune-related adverse events,irAEs)的影响。结果12例接受纳武利尤单抗治疗的患者,ADA检测结果均为阴性,最佳总体反应率(best overall response,bOR)为25.0%,中位无进展生存期(median progression-free survival,mPFS)为4.1(1.9~26.9)个月,中位总生存期(median overall survival,mOS)为12.4个(4.8~26.9)月。14例接受帕博利珠单抗治疗的患者,bOR为50.0%,mPFS为10.9(2.2~15.3)个月,mOS为19.0(3.5~29.6)个月;其中3例ADA阳性,11例ADA阴性,ADA阳性患者的mPFS(9.9 vs 12.4个月,P=0.280)、mOS(19.0 vs 29.6个月,P=0.874)以及采血后至出现影像进展的中位时间(7.5 vs 8.6个月,P=0.365)均短于ADA阴性患者;ADA阳性和阴性患者2级以上irAEs的发生率分别为66.7%和23.7%。结论ADA监测对于接受ICIs治疗的NSCLC患者有一定临床价值。帕博利珠单抗ADA阳性患者生存时间较短,但ADA状态对其bOR和irAEs的影响不显著。

关键词：非小细胞肺癌抗药物抗体免疫检查点抑制剂免疫相关不良反应

人源RNA解旋酶DDX1的表达、纯化及结构性质研究

在线全文

学校读者我要写书评

暂无评论

复旦学报（自然科学版） 2021年第4期60卷 427-436页

作者：高宝才段树燕苏晓琴纪锐李继喜复旦大学生命科学学院上海200438 复旦大学遗传工程国家重点实验室上海200438

RNA解旋酶DEAD-box(DDX)蛋白家族是目前已知的最大解旋酶家族,可以解旋RNA或者解离蛋白RNA复合物;其中DDX1蛋白参与多种细胞功能,在mRNA/rRNA加工及衰减、病毒复制及转录、激素代谢以及肿瘤发生发展等各方面发挥着重要作用.但目前有关D... 详细信息

RNA解旋酶DEAD-box(DDX)蛋白家族是目前已知的最大解旋酶家族,可以解旋RNA或者解离蛋白RNA复合物;其中DDX1蛋白参与多种细胞功能,在mRNA/rRNA加工及衰减、病毒复制及转录、激素代谢以及肿瘤发生发展等各方面发挥着重要作用.但目前有关DDX1如何识别、结合和解旋RNA,如何在各种生理病理过程中发挥功能的分子基础仍然不清楚,其核心功能区的结构尚未解析.本研究成功地在大肠杆菌中重组表达和纯化了人源DDX1蛋白,获得了全长DDX1及多个不同长度截短体(或结构域)蛋白.结合动态光散射(DLS)分析发现DDX1的N端解旋酶ATP结合区结构稳定,状态均一;C末端结构域可能介导与其他蛋白相互作用,具动态变化并容易聚集.X光小角散射实验表明,溶液中DDX1全长蛋白为单体且构象均一.二级结构预测和三维结构模型构建表明DDX1具有经典的DEAD解旋酶家族特征.

关键词： RNA解旋酶 DEAD-box蛋白1 动态光散射 X射线小角度散射同源建模