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Proteomics Profiling of Host Cell Response via Protein Expression and Phosphorylation upon Dengue Virus Infection

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Virologica Sinica 2019年第5期34卷 549-562页

作者： Meng Miao Fei Yu Danya Wang Yongjia Tong Liuting Yang jiuyue xu Yang Qiu Xi Zhou Xiaolu Zhao State Key Laboratory of Virology College of Life SciencesWuhan UniversityWuhan 430072China Hubei Key Laboratory of Cell Homeostasis College of Life SciencesWuhan UniversityWuhan 430072China Laboratory of RNA Virology Wuhan Institute of VirologyChinese Academy of ScienceWuhan 430071China

Dengue virus(DENV)infection is a worldwide public health *** date,the knowledge about the pathogenesis and progression of DENV infection is still *** global profiling based on proteomic analysis together with functional verification analysis is a powerful strategy to investigate the interplay between the virus and host *** the present study,quantitative proteomics has been applied to evaluate host responses(as indicated by altered proteins and modifications)in human cells(using K562 cell line)upon DENV-2 infection,as DENV-2 spreads most widely among all DENV *** analysis was performed to define differentially expressed proteins in the infected cells compared to the mock-control,and it revealed critical pathogen-induced changes covering a broad spectrum of host cellular compartments and *** also discovered more dramatic changes(>20%,160 regulated phosphoproteins)in protein phosphorylation compared to protein expression(14%,321 regulated proteins).Most of these proteins/phosphoproteins were involved in transcription regulation,RNA splicing and processing,immune system,cellular response to stimulus,and macromolecule *** blot analysis was also performed to confirm the proteomic *** roles of these altered proteins were *** present study provides valuable large-scale protein-related information for elucidating the functional emphasis of host cell proteins and their post-translational modifications in virus infection,and also provides insight and protein evidence for understanding the general pathogenesis and pathology of DENV.

关键词： Dengue virus(DENV) Quantitative proteomics Virus-host interaction Protein expression Protein phosphorylation

SARS-CoV-2-encoded nucleocapsid protein acts as a viral suppressor of RNA interference in cells

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Science China(Life Sciences) 2020年第9期63卷 1413-1416页

作者： Jingfang Mu jiuyue xu Leike Zhang Ting Shu Di Wu Muhan Huang Yujie Ren xufang Li Qing Geng Yi xu Yang Qiu Xi Zhou State Key Laboratory of Virology Wuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of Sciences(CAS)Wuhan 430071China Joint Laboratory of Infectious Diseases and Health Wuhan Institute of Virology&Wuhan Jinyintan HospitalCASWuhan 430023China The University of Chinese Academy of Sciences Beijing 100049China Center for Translational Medicine Wuhan Jinyintan HospitalWuhan 430040China Center for Precision Translational Medicine of Wuhan Institute of Virology and Guangzhou Women and Children’s Medical Center Guangzhou Women and Children’s Medical CenterGuangzhou 510120China Department of Thoracic Surgery Renmin Hospital of Wuhan UniversityWuhan 430060China

Dear Editor,Coronaviruses (Co Vs) are large enveloped non-segmented positive-strand RNA viruses that broadly distribute among humans and other animal species, including bats, mice and birds. SARS-Co V-2 infections can cause diseases, named the 2019 novel coronavirus disease (COVID-19). The symptoms of COVID-19 range from mild symptoms to severe respiratory syndromes, including pneumonia, and even death(Chen et al., 2020b;Jiang and Shi, 2020;Xia et al., 2020).

关键词： al. suppressor diseases

Genetic Architecture of Natural Variation in Rice Chlorophyll Content Revealed by a Genome-Wide Association Study

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Molecular Plant 2015年第6期8卷 946-957页

作者： Quanxiu. Wang Weibo Xie Hongkun Xing Ju Yan Xiangzhou Meng Xinglei Li Xiangkui Fu jiuyue xu Xingming Lian Sibin Yu Yongzhong Xing Gongwei Wang National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) Huazhong Agricultural University Wuhan 430070China

Chlorophyll content is one of the most important physiological traits as it is closely related to leaf photo- synthesis and crop yield potential. So far, few genes have been reported to be involved in natural variation of chlorophyll content in rice （Oryza sativa） and the extent of variations explored is very limited. We con- ducted a genome-wide association study （GWAS） using a diverse worldwide collection of 529 O. sativa accessions. A total of 46 significant association loci were identified. Three F2 mapping populations with parents selected from the association panel were tested for validation of GWAS signals. We clearly demon- strated that Grain number, plant height, andheading date7 （GhdT） was a major locus for natural variation of chlorophyll content at the heading stage by combining evidence from near-isogenic lines and transgenic plants. The enhanced expression of Ghd7 decreased the chlorophyll content, mainly through down- regulating the expression of genes involved in the biosynthesis of chlorophyll and chloroplast. In addition, Narrow leaf1 （NAL1） corresponded to one significant association region repeatedly detected over two years. We revealed a high degree of polymorphism in the 5＇ UTR and four non-synonymous SNPs in the cod- ing region of NAL1, and observed diverse effects of the major haplotypes. The loci or candidate genes iden- tified would help to fine-tune and optimize the antenna size of canopies in rice breeding.

关键词： chlorophyll GWAS Ghd7 NAL 1 pleiotropic effect Oryza sativa

内化到外化:中国共产党推进精神生活共同富裕的演进理路

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重庆理工大学学报（社会科学） 2024年第3期38卷 9-18页

作者：侯爱萍许九悦山东农业大学马克思主义学院山东泰安271018

精神生活共同富裕是一个国家或民族精神文明程度的重要标识,是立足于人民的精神需要与社会主义精神文明建设的现实条件,其时代意蕴在满足人民美好生活需要的基本追求中得到体现,在实现中华民族伟大复兴中得以推进,在创造人类文明新形态... 详细信息

精神生活共同富裕是一个国家或民族精神文明程度的重要标识,是立足于人民的精神需要与社会主义精神文明建设的现实条件,其时代意蕴在满足人民美好生活需要的基本追求中得到体现,在实现中华民族伟大复兴中得以推进,在创造人类文明新形态中加以升华,为中国共产党团结带领人民在价值引领、舆论引导、产品供给、共享机制和数字赋能等层面接续实现共同富裕的总体目标赋予经久不息的原动力和安如磐石的内聚力。中国共产党在推进精神生活共同富裕的道路中由内化到外化,由理论到实践,按照“内在生成—关系联结—实践外延”的演进逻辑,展现出中国共产党向往精神生活共同富裕的多重维度、追求向度和践行力度。

关键词：中国共产党精神生活共同富裕精神文明演进理路

“第二个结合”:何以必要、何以可能与何以可为

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理论与现代化 2024年第5期 96-104页

作者：马旭许九悦山东大学马克思主义学院中国地质大学(武汉)马克思主义学院

“第二个结合”是中国共产党科学审视马克思主义和中华优秀传统文化关系而提出具有原创性的理论命题。坚持马克思主义基本原理同中华优秀传统文化相结合能够不断开辟马克思主义中国化时代化新境界,推进中华优秀传统文化创造性转化、创... 详细信息

“第二个结合”是中国共产党科学审视马克思主义和中华优秀传统文化关系而提出具有原创性的理论命题。坚持马克思主义基本原理同中华优秀传统文化相结合能够不断开辟马克思主义中国化时代化新境界,推进中华优秀传统文化创造性转化、创新性发展,实现中华民族伟大复兴中国梦。“第二个结合”绝非偶然,马克思主义与中华优秀传统文化在社会理想上都体现了共同的价值追求,在内在秉性上都秉承着开放包容的文明特质,在价值取向上都展现出人民主体的价值立场,彰显了二者之间的高度耦合性。新时代新征程,唯有始终坚持以马克思主义为根本指导、传承好弘扬好中华优秀传统文化并扎根以人民为中心的生动实践,才能推进二者结合的深化发展、走深走实,不断丰富和发展人类文明新形态。

关键词：中国共产党马克思主义基本原理中华优秀传统文化

历史主动精神:中国共产党百年奋进的精神密钥

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大连海事大学学报（社会科学版） 2023年第5期22卷 120-127页

作者：侯爱萍许九悦马旭山东农业大学马克思主义学院山东泰安271018

中国共产党持续奋进的精神密钥在于始终坚持发扬历史主动精神,在时代前进的潮流中把握主动、赢得未来。中国共产党历史主动精神孕育于中华文明沃土,激发于社会主义蓬勃态势,实践于中国共产党波澜壮阔的百年历程。历史主动精神肯定了党... 详细信息

中国共产党持续奋进的精神密钥在于始终坚持发扬历史主动精神,在时代前进的潮流中把握主动、赢得未来。中国共产党历史主动精神孕育于中华文明沃土,激发于社会主义蓬勃态势,实践于中国共产党波澜壮阔的百年历程。历史主动精神肯定了党践行初心使命的人民性,体现了党推动理论守正创新的科学性,表征着中国式现代化之路的发展性,确证了中国助力人类文明进步的创新性。新时代充分发扬伟大的历史主动精神,必须坚定人民立场、强化理论武装、保持忧患意识、顺应历史规律,才能满足人民美好生活需要,弘扬历史主动精神品质,掌握时势发展的主动权,开创伟大事业崭新局面。

关键词：中国共产党历史主动精神中国式现代化

A stretchable fabric as strain sensor integrating electromagnetic shielding and electrochemical energy storage

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Nano Research 2023年第11期16卷 12753-12761页

作者： xu Li Xiaohui Sun jiuyue Zhang Song xue Linjie Zhi Advanced Chemical Engineering and Energy Materials Research Center China University of Petroleum(East China)Qingdao 266580China School of Materials Science and Engineering China University of Petroleum(East China)Qingdao 266580China College of New Energy China University of Petroleum(East China)Qingdao 266580China

Multifunctional intelligent fabric plays an integral role in health management,human–machine interaction,wireless energy storage and conversion,and many other artificial intelligence ***,we demonstrate a newly developed MXene/polyaniline(PANI)multifunctional fabric integrated with strain sensing,electrochemical energy storage,and electromagnetic shielding *** multifunctional fabric-based strain sensor possesses a real-time signal response at a sizeable tensile strain of 100%with a minute strain of 0.5%,maintaining a stable and consistent signal response even after 3000 stretch–release *** addition,the multifunctional fabric exhibits excellent electromagnetic shielding capabilities,achieving a total shielding effectiveness value of up to 43 dB,and in the meantime shows attractive electrochemical energy storage performance as an electrode in a supercapacitor,offering a maximum specific capacity and energy density of 522.5 mF·cm^(−2)and 18.16μWh·cm^(−2),*** a multifunctional intelligent fabric offers versatile opportunities to develop smart clothes for various artificial intelligent applications.

关键词： strain sensor MXene polyaniline(PANI) multifunctional intelligent fabric electromagnetic interference(EMI)shielding electrochemical energy storage

夹岩水利枢纽工程白甫河高大跨管桥拱圈锚碇结构设计与应用

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水利水电快报 2021年第7期42卷 34-39页

作者：任明武徐青松谭九月贵州省水利投资(集团)有限责任公司贵州贵阳520001 中国葛洲坝集团路桥工程有限公司湖北宜昌443002

在夹岩水利枢纽工程白甫河高大跨管桥拱圈施工过程中,为了给拱圈悬臂浇筑节段提供充足的锚固力,又可降低工程施工成本,根据两岸桩基、镇墩等永久构筑物布置,结合施工现场的实际地形、地质条件,采用了混合式锚碇结构和新型预应力锚碇结... 详细信息

在夹岩水利枢纽工程白甫河高大跨管桥拱圈施工过程中,为了给拱圈悬臂浇筑节段提供充足的锚固力,又可降低工程施工成本,根据两岸桩基、镇墩等永久构筑物布置,结合施工现场的实际地形、地质条件,采用了混合式锚碇结构和新型预应力锚碇结构。目前管桥拱圈已安全顺利合龙,施工过程中的监测数据表明锚碇结构和管桥拱圈状态正常,说明混合式锚碇结构和新型预应力锚碇结构结构设计合理,施工质量良好,结构安全可靠,并保证了拱圈的施工安全、进度和质量。

关键词：混合式锚碇结构预应力锚碇结构高大跨管桥拱圈夹岩水利枢纽工程

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带行走轴的机器人离线程序与校准方法

汽车工艺与材料 2022年第5期 67-70页

作者：王亭力魏国兴吴政勋刘久月徐海博潘彤一汽模具制造有限公司长春130013

为了提高带行走轴的机器人的现场调试效率,缩短调整周期,降低成本,基于KUKA机器人系统,应用Tecnomatix软件对带行走轴的机器人进行虚拟模拟仿真,通过多点法对其离线程序进行精度校准。实践证明,基于多点法拟合校准之后的离线路径有效提... 详细信息

为了提高带行走轴的机器人的现场调试效率,缩短调整周期,降低成本,基于KUKA机器人系统,应用Tecnomatix软件对带行走轴的机器人进行虚拟模拟仿真,通过多点法对其离线程序进行精度校准。实践证明,基于多点法拟合校准之后的离线路径有效提高了带行走轴的机器人的调试效率,缩短了调试时间。

关键词： Tecnomatix 行走轴KUKA 机器人系统离线程序

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